Objective:To analyze the characteristics of adults (non-pregnant women) infected with Listeria monocytogenes and to provide evidence for diagnosis and treatment of this disease. Methods:Patients admitted in West China Hospital of Sichuan University between January 2008 and April 2020 with Listeriadisease were enrolled in this study. The clinical data of patients including age, symptoms, underlying diseases and prognosis, as well as the laboratory results of WBC, neutrophil ratio (N%), procalcitonin (PCT), interleukin-6 (IL-6) and C-reactive protein (CRP), were obtained for analyzing the clinical infection and prognosis characteristics of the patients. Results:A total of 57 patients were included, patients were 18-83 (52.8±15.9) years old, percent of over 60 years old patients accounted for 35.09% (20/57). The incidence rate in summer season was the highest (45.61%, 26/57). There were 48 cases with comorbidities, sepsis occurred in 31 cases and meningitis in 18 cases. The time of diagnosis of Listeriosis was (6.1±2.9) days. After diagnosis, the target therapy was applied: such as carbapenem (26.32%, 15/57) and penicillins (22.81%, 13/57). The levels of WBC (6.77 [2.99, 9.54]×10 9/L vs 10.23 [6.71, 16.55]×10 9/L), NLR (3.07 [1.66, 8.16] vs 11.26 [5.66, 20.08]) and CRP (40.7 [16.9, 91.9] g/L vs 92.8 [59.9, 142.7] g/L) were significantly lower after treatment than those before treatment in 47 hospitalized patients (all P<0.05). Thirty-eight patients were treated according to the guidelines, 33 cases improved, 2 cases had poor prognosis and 2 cases died. Conclusion:The main risk factors of Listeria monocytogenes infection in adults (non-pregnant women) are underlying diseases, especially autoimmune diseases and pulmonary infections. Penicillins can be used as the first choice for empirical therapy. Carbapenems and erythromycin serve as the combination medications during the full course of treatment.

Objective To evaluate the nucleated red blood cell (NRBC)count of Sysmex XN-9000 automatic hematology anal-ysis lines comparing with manual method,and verify the accuracy of the analyzer results.Methods 60 blood samples with more than 1% of NRBCs detected by XN-9000 were counted NRBCs by traditional manual microscopy in blood smears,and verified the analyzer results.Results According to the reliability analysis,the results of total 60 samples were all within the range of the reliability;correlation analysis showed that correlation coefficient (r)of group NRBC (%)1~10 and>10 were 0.972 1 and 0.996 2,respectively.There were no significant differences between them (P>0.05).Conclusion Compared with manual method,the results of NRBC count of XN-9000 were within the range of reliability,and showed good correla-tion.The analyzer test results of NRBC were accurate and reliable and could be applied to the detection of clinical samples.

Objective:To study the impact of perioperative intestinal microecological intervention on abdominal infection after liver transplantation.Method:Fifty patients who underwent liver transplantation at the Department of Hepatobiliary Surgery, Ningbo Medical Center Lihuili Hospital from June 2017 to June 2019 were retrospectively studied. There were 34 males and 16 females. The average age was 47 years. These patients were divided into two groups using the method of non-randomized concurrent control. One group formed the perioperative intestinal microbiological intervention group, while the other group served as the conventional treatment control group. There were 25 patients in each group. The preoperative and postoperative intestinal flora and the occurrence of postoperative abdominal infection of the two groups were compared.Results:After operation in both groups, enterococci increased, enterobacteriaceae decreased, while non-fermenting bacteria and candida showed no significant changes. In the intervention group, the counts of enterobacter, enterococcus and candida were significantly lower than the control group on day 15 after operation ( P<0.05). The contents of bifidobacteria and lactobacillus in the intervention group were significantly higher than that in the control group on days 7 and 15 after operation. On the other hand, the contents of enterobacter on day 7 and enterococcus on day 15 after operation in the intervention group were significantly lower than the control group ( P<0.05). The intestinal flora of the two groups were imbalanced before liver transplantation. The ratio of bifidobacterium / enterobacteriaceae in the intervention group was significantly higher than the control group on day 7 and 15 after operation ( P<0.05). The incidence of intestinal bacterial translocation in the control group was 32.0% (8/25), with 6 patients diagnosed to have abdominal infection caused by intestinal bacterial translocation. The incidence of intestinal bacterial translocation was 8% (2/25) in the intervention group, with 1 patient diagnosed to have abdominal infection caused by intestinal bacterial translocation. The incidence of bacterial translocation in the intervention group was significantly lower than the control group ( P<0.05). There were no significant differences in the incidences in the intestinal bacterial translocation and abdominal infection between the two groups ( P>0.05). Conclusions:Probiotics intervention in the perioperative period of liver transplantation improved the imbalance of intestinal flora, reduced the incidence of intestinal bacterial translocation, and played a positive role in reducing abdominal infection after liver transplantation.