Advanced hospitals and high-level hospitals are different from grass-root hospitals,since they not only treat difficult diseases but also have the obligation of scientific studies and teaching.Grass-root hospitals mainly focus on treating common diseases,and only conduct a small amount of teaching,thus resulting in the difference between grass-root hospitals and large-scale hospitals in the aspect of purchasing medical equipment.Because the money for grass-root hospitals may only be used as designated and some even comes from loan or collecting,we have to pinch pennies and make the best use of everything so as to recover the cost rapidly and bring the equipment into full play.

Objective: To investigate the effects of immunotherapy on PRA, TGF-β and CD4+CD25+Foxp3+ nTreg cells in women with unexplained recurrent spontaneous abortion. Methods:We detect the level of PRA,TGF-βin serum and the expression of nTreg cells in peripheral blood of pregnant, non pregnant and natural fertility women by enzyme immunoassay and flow cytometry. Results:After immunotherapy,the median level of PRA and TGF-βin non pregnant group was lower than that in pregnant group(27. 5%,107. 7 pg/L vs 68. 75%,189. 9 pg/L,P<0. 01). Compared to pregnant group,the median expression rate of nTreg cells were lower in non pregnant group(5. 05% vs 8. 05%,P<0. 01). Conclusion:The PRA,TGF-βand nTreg cells are critical factors for pregnancy in RSA patients. It is helpful to identify and judge the effect of immunotherapy by detect the level of panel reactive antibodies,TGF-β in serum and the expression rate of nTreg cells in peripheral blood.

Cancer related anemia( CRA)is one of the ancer complications. The incidence rate of CRA is more than 70% in patients after receiving chemotherapy,radiation therapy,or both. CRA has severe clinical symptoms which significantly attenuate effectiveness of cancer treatment and the quality of patients' life. The current treatments for CRA,such as transfusion,recombinant EPO therapy,iron supplement and so on,could correct CRA in some sense. However,the present accepted therapeutic approaches could not be satisfied since there are complexity and diversity factors accounting for CRA. More effective and safety treatments of CRA are required in the near future.

The studies on the antihypoxia mechanism of 18?-SGA were reported. The survival time of hypoxia mice were longer than those of the control after intraperitoneal injections of 14mg/kg or 21mg/kg of 18?-SGA ( Sodium 18?-glycyrrenetic acid). Changes of various physiological indications after administration of the drug were as follows: blood pH was risen; acidosis was relieved; and the partial pressure of oxygen in the mixed blood was decreaed. The blood thyroxine level remain unchanged. It suggests the utilization of oxygen by the tissues was strengthened.

Objective To explore changes in glycogen synthase kinase-3 (GSK-3) levels in the lymphocytes of patients with mild cognition impairment (MCI) and Alzheimer's disease (AD). Methods GSK-3 activity was measured by 32P liquid scintillography in a control group, an MCI group and an AD group. The expressions of GSK-3βat Ser9 and total GSK-3β were determined by western blotting. Results GSK-3 levels were significantly higher in the AD and MCI groups than in the control group. The phosphorylation of GSK-3βat Ser9 was significantly lower in the AD and MCI groups compared with the control group, but there was no difference in the phosphorylation of total GSK-3β among the three groups. Conclusions Higher GSK-3 activity in the lymphocytes might have value in the diagnosis of MCI and early AD.

AIM: To observe the inhibitory effect of interferon-α ( IFN-α) on the growth invasiveness and metastasis of human gastric carcinoma cell line BGC-823, and mechanism of its action. METHODS: We detected the influence of IFN-α on the proliferative ability of BGC-823 in cell culture system, the cell vitality with the MTT colorimetric assay, and the cell cycle with flow cytometer (FCM). The regulatory functions of IFN-α to the expression of E-cadherin and matrix metalloproteinase-2 ( MMP-2) in tumor cells were estimated by immunohistochemical analysis ( S-P). The ultrastructural changes of the junction among the tumor cells were observed under electron microscope. RESULTS : IFN-α can significantly inhibit the growth of human gastric carcinoma cell line BGC-823 in a dose-dependent manner. When the concentration of IFN-α was ≥106 U/L, the cell proliferation can be effectively suppressed,the suppression rate was ≥ 12. 2%, and the blockage appeared at the phase of G1-S of the cell cycle. Under the induction of IFN-α, the expression level of the cell E-cadherin increased while the MMP-2 decreased. The changes on ultrastructure of the cells showed the increased adhesive junctions and the relative compact structure. CONCLUSION: IFN-α can suppress the growth of human gastric carcinoma cell line BGC-823 through its influence on cell cycle. IFN-α can regulate the expression of E-cadherin and MMP-2, make the cell junction closely, so that it has the potential on restricting the invasion and metastasis of gastric carcinoma cells.

Purpose To investigate the expression of IGF-Ⅱand IGFBP-6 in co1orecta1 cancer,and to exp1ore the c1inica1 significance in co1orecta1 cancer. Methods IGF-Ⅱand IGFBP-6 were detected by immunohistochemistry and RT-PCR in 50 cases of co1orecta1 cancer(experimenta1 group)and 50 cases of the adjacent mucosa(contro1 group). Results (1)In the experimenta1 group,the ex-pression 1eve1 of IGF-Ⅱprotein and mRNA was significant1y higher than the contro1 group. The expression 1eve1s of IGF-Ⅱprotein and mRNA in co1orecta1 cancer were significant1y 1ower than the contro1 group.(2)The expression 1eve1s of IGF-Ⅱand IGFBP-6 were sig-nificant1y different between different tumor infi1tration depth,1ymph node metastasis,invasion depth and Duke’s stages( P<0. 05), but no difference between genders,age and the degree of tumor differentiation( P>0. 05). Conclusions There is a obvious corre1a-tion between of IGF-Ⅱ and IGFBP-6 in c1inica1 patho1ogica1 parameters in co1orecta1 cancer. Combined detection of the two markers may be the bio1ogica1 indicators of occurrence and prognosis of co1orecta1 cancer,and provide a new scheme for diagnosis and treatment of co1orecta1 cancer.

Prostatic disorders are common and frequently occur in male,which have caught wide attention in society.At present,the medication,surgery treatment and physical therapy are the main treatments for prostatic disorders.In this article,a new-type apparatus for prostate therapy is introduced.With radio frequency current on affected part,the abnormal tissues' temperature is raised so that the accelerated blood circulation can kill the pathogenic bacteria for cure.At the same time,there is a shaking device and a magnetostatic field in its probe,which can hasten the excretion of putrescence and desquamative tissues in the pathological parts with other normal tissues unaffected.It is worth popularizing for its safety and effectiveness.

AIM:To observe the inhibitory effect of interferon-?(IFN-?)on the growth invasiveness and metastasis of human gastric carcinoma cell line BGC-823,and mechanism of its action.METHODS:We detected the influence of IFN-? on the proliferative ability of BGC-823 in cell culture system,the cell vitality with the MTT colorimetric assay,and the cell cycle with flow cytometer(FCM).The regulatory functions of IFN-? to the expression of E-cadherin and matrix metalloproteinase-2(MMP-2)in tumor cells were estimated by immunohistochemical analysis(S-P).The ultrastructural changes of the junction among the tumor cells were observed under electron microscope.RESULTS:IFN-? can significantly inhibit the growth of human gastric carcinoma cell line BGC-823 in a dose-dependent manner.When the concentration of IFN-? was ≥106 U/L,the cell proliferation can be effectively suppressed,the suppression rate was ≥12.2%,and the blockage appeared at the phase of G_1-S of the cell cycle.Under the induction of IFN-?,the expression level of the cell E-cadherin increased while the MMP-2 decreased.The changes on ultrastructure of the cells showed the increased adhesive junctions and the relative compact structure.CONCLUSION:IFN-? can suppress the growth of human gastric carcinoma cell line BGC-823 through its influence on cell cycle.IFN-? can regulate the expression of E-cadherin and MMP-2,make the cell junction closely,so that it has the potential on restricting the invasion and metastasis of gastric carcinoma cells.