Objective To investigate the proliferation inhibitory effect and mechanism of Ginkgo Biloba extract (EGB) on non-small cell lung cancer (NSCLC) PC-9 cells. Methods Concentrations of EGB were set as 70 mg/L, 105 mg/L, 140 mg/L, 210 mg/L, and 280 mg/L, and were used to culture PC-9 cells in vitro for 24 h, 48 h, and 72 h. Tetramethylazo thiazole blue staining (MTT) method was applied to detect cell inhibition rate. Hoechst33258 fluorescent staining was employed to observe the nucleus. SOD activity and MDA content were detected by ELISA. The protein expressions of Caspase-3, Caspase-9, Cyt-C, and AIF were detected by Western blot. Results After incubated for 24 h, 48 h, and 72 h, EGB inhibited the proliferation of PC-9 cells, IC50 was 195.45 mg/L, 179.63 mg/L, 142.23 mg/L, respectively). 105 mg/L, 140 mg/L, 210 mg/L EGB could induce apoptosis of PC-9 cells, cause the nucleus pycnosis, produce apoptotic bodies, improve SOD activity and decrease MDA content (P<0.05, P<0.01, P<0.001). Western blot results showed that, compared with the control group, EGB can obviously increased the protein expressions of Caspase-3, Caspase-9, Cyt-C, and AIF, with statistical significance (P<0.05, P<0.01, P<0.001). Conclusion EGB can effectively restrain proliferation of non-small cell lung cancer PC-9 cells, the mechanism may be realized by inducing PC-9 cell apoptosis through pathway of mitochondria apoptosis, reducing oxidation, and clearing free radicals.

Objective To investigate the effects of ligustilide on the proliferation of human lung adenocarcinoma A549 cells and its mechanism. Methods CCK-8 method was used to detect the effects of ligustilide on activity of A549 cells. Apoptosis rate was measured by TUNEL. Nuclear morphological changes of A549 cells were observed by fluorescence microscope after staining by Hoechst 33258. ELISA was used to detect the VEGF levels after incubation with ligustilide. Western blot was used to detect the expression of NF-κB, Bcl-2, Bax and the protein expression of phosphorylation of JNK. Results A549 cell activity was significantly inhibited after incubated with 15, 30, 60, 120, 180μmol/L ligustilide for 12, 24, 48 h (P<0.05, P<0.01, P<0.001) in a dose and time-dependent manner (P<0.05, P<0.01);Apoptosis rate increased by 30, 60, 120 μmol/L ligustilide for 12, 24, 48 h in a dose and time-dependent manner;A549 cells treated with ligustilide for 48 h appeared the apoptosis forms, including nuclear shrinkage, beating, strong blue fluorescence staining, and the chromatin divided producing apoptotic body;30, 60, 120μmol/L ligustilide increased the expression of p65 subunit of NF-κB protein in the nuclei and the phosphorylation levels of JNK protein, reduce the expression of suppression apoptosis protein Bcl-2, and raised the expression of pro-apoptotic protein Bax (P<0.05, P<0.01, P<0.001). Conclusion By inhibiting the expression of VEGF, ligustilide can inhibit the formation of tumor angiogenesis. By regulating the expression of NF-κB and the phosphorylation levels of JNK protein and reducing the ratio of Bcl-2/Bax, ligustilide can promote tumor cell apoptosis.

OBJECTIVE:To investigate inhibitory effects of matrine on the proliferation of human bladder cancer BIU-87 cells and its mechanism. METHODS:The cell viability was detected by MTT assay and inhibitory rate of cell proliferation was calculat-ed after human bladder cancer BIU-87 cells were treated with 0(negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 24,48 and 72 h,respectively. After treated with 0 (negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 48 h,the cell cycle and apoptotic rate were detected by flow cytometry;the expression of Survivin,Caspase-3 and Caspase-7 protein were detected by Western blot assay. RESULTS:Compared with negative control,the proliferation of BIU-87 cells were significantly inhibited after incubated with 1.0-4.0 mg/ml matrine for 24,48 and 72 h(P<0.05 or P<0.01),and inhibitory rate of cell proliferation increased in concentration and time-dependant manner;after treated for 48 h,the percentage of G0/G1 phase cells and apoptotic rate in-creased,while the percentage of cells at S phase and G2/M phase were decreased (P<0.05 or P<0.01);the expression of Cas-pase-3 and Caspase-7 protein increased,while the expression of survivin protein decreased after incubated with 0.5-4.0 mg/ml ma-trine for 48 h. CONCLUSIONS:Matrine can inhibit the proliferation of human bladder cancer BIU-87 cells,block the cell cycle and induce apoptosis;its mechanism may be related to the expression regulation of Survivin,Caspase-3 and Caspase-7.

AIM: To investigate the effects of Liucha extract on the growth of tumor cells in vitro and its possible mechanism. METHODS: The capability of colony forming of human leukemia K562 cell in vitro and the cells metabolism were studied by semi-solid agar culture and MTT staining. Then , the changes in morphology in the tumor cells were examined using electronic microscope. RESULTS: Semi-solid agar culture and MTT colorimetric analysis showed that Liucha extrats could significantly inhibit the growth of the tumor cells and their capability of colony forming. Also,under the electronic microscope,it was found that the tumor K562 cell had a narrower perinuclear space,condensation of chromatin and an enlarged mitochondria , in which the cristase disappeared. CONCLUSION: The extract from Liucha possesses an inhibitory effect on K562 cell growth in vitro through affecting the metabolism of the tumor cells.

Objective To evaluate whether the forced expiratory volume in sixth scend(FEV6) was the optimal surrogate for forced vital capacity (FVC) in the diagnosis for chronic obstructive pulmonary disease (COPD).Methods Retrospectively analyzed the spirometric data of 142 COPD patients (7 cases with mild COPD,60 cases with moderate COPD,48 cases with severe COPD,27 cases with extremely severe COPD) admitted to Hebei Chest Hospital from October 2011 to October 2012 who had FEV6 data.FEV6,FEV5,FEV4 and FEV3 were measured on volume-time curves and the diagnostic value was analyzed.Results FEV6,FEV5,FEV4 and FEV3 were highly correlated to FVC (r =0.994,0.939,0.935 and 0.923 respectively,P ＜0.001).Assuming =70％ as the diagnostic standard for obstruction,FEV1/FEV6 had a diagnostic rate of 92.96％ with a false negative rate of 7.04％.FEV1/FEV5,FEV1/FEV4 and FEV1/FEV3 had higher false negative rates (9.86％,13.38％ and 27.46％ respectively) than FEV6.The value of FEV1/FEV6-FEV1/FVC had no statistical significance between the mild-moderate and the severe-extremely severe COPD groups(t =1.376,P =0.171).Conclusion There is a strong correlation between FEV6 and FVC.FEV6 may be the best surrogate for FVC in the diagnosis of chronic obstructive pulmonary disease.Elevating diagnostic critical value can reduce the false negative rate.

Objective:To explore the effect of Jiudaiqingyuan oral liquid in strengthening immunologic function of children with RRI.Methods:100 children with RRI were randomly divided into two groups:the routine treatment group(RTG) and the group treated with Jiudaiqingyuan oral liquid (JDG).Venous blood was drawn before and after treatment to detect T lymphocyte subsets(SP method),NK cell activity (MTT method),sIL 2R(ELISA method),IgG,IgA,IgM(transmission turbidimetric method).Results:CD3 +,CD4 +,CD4 +/CD8 +,NK cell activity and IgG,IgA of the children with RRI were notably lower and CD8 +、IgM、sIL 6R markly higher before treatment.These indexes recovered to some extent after routine treatment but were still significantly different from those of normal controls(P0 05).Conclusion:Jiudaiqingyuan oral liquid has strong effect on immunologic regulation.It may markly enhance cellular and humoral function of children with RRI.

Objective The purpose of this study was to determine the efficacy of forced expiratory volume in six seconds(FEV6) as an alternative for forced vital capacity(FVC)and the fixed eat-off points for FEV1/FEV6 in the diagnostic screening for chronic obstructive pulmonary disease (COPD).Methods From August 2007 to December 2008,a total of 1210 spirometric examinations in were analyzed,FEV6 was measured on volume-time curves.The correlation between FEV1/FVC and FEV1/FEV6 was evaluated by the Kendall correlation test.Considering FEV1/FVC

ObjectiveTo investigate the clinical therapeutic effect and safety of solifenacin suceinate in the treatment of overactive bladder symptom after benign prostatic hyperplasia (BPH)operation.MethodsAmong 115 patients receiving BPH surgery,58 cases were given solifenacin at bedtime 4 d after surgery for 20 d (treatment group),and 57 cases were given anisodamine after surgery (control group).The urination of patients before and after pulling out catheter were observed,and urodynamic examination,international prostatic symptoms score (IPSS) and overactive bladder symptom score (OABSS) were used to evaluate urination status.ResultsIn treatment group,IPSS and OABSS decreased from 28.3 to 11.3 score and from (14.2±1.2) to (2.9±0.7) score before and after treatment,respectively (P＜0.01).In control group,IPSS and OABSS decreased from 27.3 to 11.8 score (P＜0.01) and from (14.2±1.6) to (11.3±1.1) score before and after treatment,respectively (P＞0.05).ConclusionsThe proper use of solifenacin after prostate operation may release bladder distress and facilitate rehabilitation in patients with overactive bladder symptom.

Objective To study seasonal variation of blood pressure (BP) in patients with essential hypertension (EH) and its association with meteorological elements. Methods In total, 1800 patients with essential hypertension were recruited by cluster sampling from three townships and towns in Qingzhou,Shandong province, 1155 men and 645 women, with an average age of 61.3 years (ranging from 29 to 85years). BP was measured for all of them in sitting position at 8:00 in the morning every seven to 14 days during July 2008 to June 2009. Local meteorological data were collected accordingly such as temperature,humidity and atmospheric pressure, and multivariate regression analysis was performed to show their association. Results In general, BP in patients with EH presented a decreasing trend during the first half year of observation, with the lowest in the summer ( May to August). From September to October, their BP began to increase gradually, and reached the peak in the winter ( November to February nest year). There was significant difference in systolic BP (SBP) and diastolic BP (DBP) between various seasons (Pspring-summer = 0.002 and 0.000, Pwinter-spring = 0.001 and 0.000, Psummer-autumn = 0.045 and 0.000,Psummer-winter =0. 000 and 0. 000, Pautumn-winter =0. 000 and 0. 000, respectively), except for those between the spring and autumn. Both SBP and DBP reversely associated with outdoor air temperature and room temperature(beta= -0.08, t = -2.39, P ＜0.05 and beta = -0.24, t = -6.21, P ＜0.01,respectively), (beta = -0.08, t = -2.39, P ＜0.05 and beta = -0.24, t = -6.21, P ＜0.01,respectively). Conclusions BP in patients with EH reveals seasonal variation, with the lowest in the summer and the highest in the winter and evident fluctuation in the spring and autumn, especially in SBP,which associates with room temperature and their blood vessel elasticity.

Objective To explore the antibacterial effect and mechanisms of platelets (PLT)on Staphylococcus epidermidis (SE).Methods Built infectious model of SE in vitro,whose final concentration was 105 CFU/ml in the reaction.Separately cocultured these models with PLT (the final concentration 400 × 109/L)-plasma (positive control)and BHI medium 12 hours later,and detected the antibacterial effect of PLT by making a liquid thinner and spreading counting method,and draw-ing antibacterial curve.Meanwhile,observed the bacterial structure by transmission electron microscope (TEM),and initially explored the antibacterial mechanism of PLT.Results The study showed the antibacterial effect of PLT on SE was very ob-vious,which appeared later than the plasma (M)group,but enduring.The images of TEM showed an electronlight region appeared in the centre of bacterium,contained condensed DNA molecules and leaded to slower fission.Conclusion PLT can damage the DNA structure of SE,and then affect the fission of SE,finally inhibit the proliferation of SE.