1.Cell culture and identification of pericytes in rat brain.
Qiang LI ; Wen-yan LI ; Bo LI ; Yu-xing HUANG ; An-yong YU ; Hong-fei GE ; Yun-feng YANG ; Rong HU ; Huai-zhen RUAN ; Guo-cai WU ; Wei-hua TANG ; Hua FENG
Chinese Journal of Pathology 2013;42(8):551-553
2.Characterization of atherosclerotic plaque in patients with unstable angina pectoris and stable angina pectoris by optical coherence tomography.
Bu-xing CHEN ; Feng-yun MA ; Wei LUO ; Jian-hong RUAN ; Xi-zhe ZHAO ; Wen-li XIE ; Shu-hong SUN ; Xu-mei GUO ; Feng WANG ; Ting TIAN ; Xiao-wen CHU
Chinese Journal of Cardiology 2009;37(5):422-425
OBJECTIVETo compare the characterization of coronary atherosclerotic plaques in patients with unstable angina pectoris (UAP) and stable angina pectoris (SAP) by optical coherence tomography (OCT).
METHODSOCT was performed in 47 patients (23 UAP and 24 SAP) undergoing coronary angiography. Lipid-rich plaque (defined by > or = 2 quadrants of the cross-section area), thin cap fibroatheroma (TCFA), thickness of fibrous cap, plaque rupture, calcification and thrombus visualized by OCT were compared between UAP and SAP patients.
RESULTSOCT imaging was successfully in 44 out of 47 patients (22 UAP, 22 SAP). Proportion of lipid-rich plaques was similar between UAP and SAP groups [91% (20/22) vs. 73% (16/22), P = 0.741]. The minimum thickness of fibrous cap in the UAP group was significantly thinner than that in SAP group [(69.5 +/- 34.7) microm vs. (141.1 +/- 68.5) microm, P = 0.000] and the rate of fibrous cap erosion in the UAP group was significantly higher than that in the SAP group [59% (13/22) vs. 9% (2/22), P = 0.000]. Percents of TCFA [73% (16/22) vs. 14% (3/22), P = 0.000] and plaque rupture [50% (11/22) vs. 9% (2/22), P = 0.003] were significantly higher in UAP group compared those in SAP group. Incidence of thrombus and calcification were similar between two groups.
CONCLUSIONSOCT imaging can clearly define plaque characterization of coronary atherosclerosis. UAP patients have thinner fibrous cap, higher incidences of fibrous cap erosion, plaque rupture and TCFA compared patients with SAP.
Aged ; Angina Pectoris ; diagnostic imaging ; Angina, Unstable ; diagnostic imaging ; Coronary Artery Disease ; diagnostic imaging ; Female ; Humans ; Male ; Middle Aged ; Radiography ; Retrospective Studies ; Tomography, Optical Coherence
3.Study on the threshold of HIV-1 drug resistance in Hunan province.
Xi CHEN ; Hui XING ; Jian-mei HE ; Jun ZHENG ; Xiao-bai ZOU ; Yu-hua RUAN ; Bi-yun QIN ; Yi-ming SHAO
Chinese Journal of Epidemiology 2008;29(8):787-789
OBJECTIVETo survey the prevalence of HIV-1 drug resistance in patients newly diagnosed as HIV positive and who were younger than 25 years of age but had not received treatment with antiretroviral treatment (ART) in Hunan province.
METHODSSerum specimens of HIV infected individuals were collected and HIV-1 pol genetic mutations associated with drug resistance were identified with PCR and interpreted with Stanford HIV Drug Resistance Database.
RESULTSA total number of 69 patients whose HIV sequences were amplified successfully with 2 (2.9%) specimens appeared mutations associated with HIV-1 drug resistance in the reverse transcriptase region, including one as V75M and the other one as K103N and V181C. Data showed that the prevalence of drug resistance in Hunan was 2.9%.
CONCLUSIONThe first line regiments of national ART seemed still effective. Most of the AIDS patients did not need to be tested on drug resistance status before starting ART. However, it is important to start HIV drug resistance surveillance in no time.
Adolescent ; Adult ; Anti-HIV Agents ; pharmacology ; Child ; Child, Preschool ; China ; Cross-Sectional Studies ; Drug Monitoring ; Drug Resistance, Viral ; Female ; HIV Infections ; blood ; drug therapy ; HIV-1 ; drug effects ; Humans ; Male ; Prevalence ; Young Adult
4.A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues.
Jing LI ; Hai-yan ZHAO ; Xing-yun RUAN ; Yong-qing XU ; Wei-zheng MENG ; Kun-peng LI ; Jing-qiang ZHANG
Journal of Zhejiang University. Science. B 2005;6(12):1210-1212
A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fourier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform simultaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction segmentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.
Algorithms
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Anatomy, Cross-Sectional
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methods
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Artificial Intelligence
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Image Enhancement
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methods
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Image Interpretation, Computer-Assisted
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methods
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Imaging, Three-Dimensional
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methods
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Pattern Recognition, Automated
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methods
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Reproducibility of Results
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Sensitivity and Specificity
5.Diagnostic value of 128-slice CT coronary angiography in comparison with invasive coronary angiography.
Bu-Xing CHEN ; Feng-Yun MA ; Zhi-Yong WEN ; Wei LUO ; Xi-Zhe ZHAO ; Feng KANG ; Quan-Hong ZHOU ; Jian-Hong RUAN
Chinese Journal of Cardiology 2008;36(3):223-228
OBJECTIVETo observe the diagnostic value of non-invasive 128-slice computed tomography coronary angiography (CTA) in comparison with invasive coronary angiography.
METHODS128-slice CTA and invasive coronary angiography were performed in 78 unselected consecutive patients (63 patients with suspected coronary artery disease and 15 patients with previous coronary stenting, 56 males, mean age 61 +/- 10 years) and > 50% reduction of minimal lumen diameter was defined as significant coronary stenosis.
RESULTSFifty-eight out of 879 segments (7%) from CTA were not assessable because of irregular rhythm, vessel calcification or tachycardia. Compared with invasive coronary angiography, segment-based analysis from the 821 segments showed the sensitivity by CTA was 87%, specificity 97%, PPV 83% and NPV 97%. Four out of 22 stents implanted in 15 patients were not assessable by CTA because of poor image quality. Compared with invasive coronary angiography, the sensitivity of diagnosing in-stent restenosis by CTA was 100%, specificity 77%, PPV 63% and NPV 100% for the remaining 18 stents.
CONCLUSIONSOne hundred and twenty-eight-slice CTA has a high accuracy for detecting coronary artery disease and in-stent restenosis after coronary stenting and could be considered as a valuable noninvasive technique for screening coronary artery disease in suspected patients.
Adult ; Aged ; Coronary Angiography ; methods ; Coronary Artery Disease ; diagnosis ; diagnostic imaging ; Coronary Stenosis ; diagnosis ; diagnostic imaging ; Female ; Humans ; Male ; Middle Aged ; Tomography, Spiral Computed ; methods
6.Epidemiology, clinical and laboratory characteristics of currently alive HIV-1 infected former blood donors naive to antiretroviral therapy in Anhui Province, China.
Jian-qing XU ; Jian-jun WANG ; Li-feng HAN ; Chen XU ; Yu-hua RUAN ; Zhen-hou XU ; Xi CHEN ; Zhen-dong LIU ; Jun WANG ; Bing SU ; Xin-ping DING ; Bing GAO ; Yong-bin GU ; Xiao-yun CAO ; Hui XING ; Kun-xue HONG ; Hong PENG ; Quan-bi ZHAO ; Lin YUAN ; Yi FENG ; Gui-yun ZHANG ; Li-ying MA ; Lan WU ; Yi-ming SHAO
Chinese Medical Journal 2006;119(23):1941-1948
BACKGROUNDUnregulated commercial blood/plasma collection among farmers occurred between 1992 and 1995 in central China and caused the second major epidemic of human immunodeficiency virus type 1 (HIV-1) infection in China. It is important to characterize HIV-1-infected former blood donors and to study characteristics associated with disease progression for future clinical intervention and vaccine development.
METHODSA cross-sectional study was performed on HIV-1-infected former blood donors (FBDs) and age-matched HIV-seronegative local residents. Demographic, epidemiologic, clinical and key laboratory data were collected from all study participants. Both unadjusted and adjusted multivariate linear regressions were employed to analyze the association of the decrease of CD4(+) T-cell counts with other characteristics.
RESULTSTwo hundred and ninety-four HIV-1-infected FBDs and 59 age-matched HIV-seronegative local residents were enrolled in this study. The unregulated blood/plasma collection occurred more than a decade (10.8 - 12.8 years) ago, which caused the rapid spread of HIV-1 infection and the high prevalence of co-infection with hepatitis C virus (HCV, 89.5%); hepatitis B virus (HBV) co-infection was observed in only 11 HIV(+)participants (3.7%). Deterioration in both clinical manifestation and laboratory parameters and increase of viral loads were observed in parallel with the decrease of CD4(+) T-cell counts. The decrease of total lymphocyte counts (P < 0.001) and hemoglobin levels (P < 0.001) and the appearance of dermatosis (P = 0.03) were observed in parallel with the decrease of CD4(+) T-cell counts whereas viral loads (P < 0.001) and CD8(+) T-cell counts (P = 0.01) were inversely associated with CD4(+) T-cell counts.
CONCLUSIONSCo-infection with HCV but not HBV is highly prevalent among HIV-1-infected FBDs. CD4(+) T-cell counts is a reliable indicator for disease progression among FBDs. Total lymphocyte counts, hemoglobin level and appearance of dermatosis were positively associated with CD8(+) T-cell counts and viral loads were inversely associated with the decreased CD4(+) T-cell counts.
Adult ; Aged ; Blood Donors ; China ; epidemiology ; Cross-Sectional Studies ; Female ; HIV Infections ; complications ; epidemiology ; immunology ; HIV-1 ; Hepatitis C ; complications ; Humans ; Male ; Middle Aged
7.Mechanism of Electroacupuncture on Preventing Apoptosis of Hippocampal Neurons in Rats with Acute Sleep Deprivation and Its Effect on Anxiety
Bo-yu JIAO ; Fan-qi MENG ; Xing-ru XIAO ; Jing-yun ZHANG ; Wen-ya PEI ; Jing-wen RUAN
Journal of Sun Yat-sen University(Medical Sciences) 2022;43(6):958-966
ObjectiveTo investigate the mechanism of Sishencong electroacupuncture on preventing the apoptosis of hippocampal neurons in rats with acute sleep deprivation and its effect on anxiety behavior. MethodsAdult Sprague-Dawley (SD) rats were randomly divided into Sham group (control group), SD group (sleep deprivation group), and EA+SD group (eletroacupuncture group). Modified multiple platform method was used to induce sleep deprivation for 4 days. EA+SD group received Sishencong electroacupuncture in each day during sleep deprivation with 20 minutes needling for 4 days. The open field test was used to detect the exploration of rats to evaluate the degree of anxiety, and the co-labeling of NeuN+ and Cleaved Caspase-3 in different regions of the hippocampus was detected by immunofluorescence staining; the astrocyte marker glial fibrillary acidic protein (GFAP) in the hippocampus was labeled by immunofluorescence. GFAP and the morphological changes of astrocytes were analyzed by Sholl analysis, and the co-labeling with C3+ and S100A+ were detected to analyze the A1 and A2 types of astrocytes in the hippocampus. ResultsOpen field test showed that the time spent in the center of rats was increased in EA+SD group, but the movement speed was decreased compared with SD group. Immunofluorescence staining confirmed that the expression of Cleaved caspase-3 on neurons in different regions of the hippocampus (CA1, CA2, CA3, DG1, DG2) in the EA+SD group was lower than that of the SD group (P<0.05). The number of intersections of astrocytes and concentric circles in the EA+SD group at 12 μm,15 μm, 18 μm and 21 μm were higher than those of the SD group. Immunofluorescence showed that electroacupuncture could regulate the proportion of C3+/GFAP+ and S100A+/GFAP+ in the hippocampus of rats during sleep deprivation. ConclusionElectroacupuncture could improve the exploration during sleep deprivation by reducing the apoptosis of neurons in the hippocampus, repairing the morphological damage of astrocytes and balancing the proportion of A1 and A2 astrocytes in the hippocampus.
8.A cohort study on the association between resting heart rate and the risk of new-onset heart failure.
Hong Min LIU ; Shuo Hua CHEN ; Yun Tao WU ; Xiao Ming ZHENG ; Zhe HUANG ; Xing LIU ; Xiao Hong ZHAO ; Hai Yan ZHAO ; Chun Yu RUAN ; Chang Hao ZU ; Yang Yang WANG ; Shou Ling WU
Chinese Journal of Cardiology 2020;48(5):413-419
Objective: To prospectively explore the relationship between resting heart rate (RHR) and risk of new-onset heart failure. Methods: It was a prospective cohort study. People who attended the physical examination of Kailuan Group Company in 2006 and with complete electrocardiography (ECG) recordings were eligible for this study. A total of 88 879 participants aged 18 years old or more who were free of arrhythmia, a prior history of heart failure and were not treated with β-blocker were included. Participants were divided into 5 groups according to the quintiles of RHR at baseline (Q(1) group, 40-60 beats/minutes (n=18 168) ; Q(2) group, 67-70 beats/minutes (n=18 970) ; Q(3) group, 71-74 beats/minutes (n=13 583) ; Q(4) group, 75-80 beats/minutes (n=22 739) ; and Q(5) group,>80 beats/minutes (n=15 419) ) .The general clinical data and laboratory test results were collected. The outcome was the first occurrence of heart failure at the end of follow-up (December 31, 2016) .We used Cox regression model to examine the association between RHR and the risk of new-onset heart failure. Hazard ratio (HR) with 95% confidence intervals (CI) were calculated using Cox regression modeling. Results: Among the included patients 68 411 participants were male, mean age was (51.0±12.3) years old, and RHR was (74±10) beats/minutes. Statistically significant differences among the RHR quintiles were found for the following variables: age, gender, systolic blood pressure, diastolic blood pressure, triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, fasting blood glucose, body mass index, the level of high-sensitivity C-reactive protein, education status, physical activity, smoking status, drinking status, history of diabetes, history of hypertension and history of use antihypertensive drugs (all P<0.01) . Higher RHR was linked with higher prevalence of diabetes, hypertension history, and higher systolic blood pressure, diastolic blood pressure and FBG levels (all P<0.01). After a mean follow-up of 9.5 years, the incidence of new-onset heart failure in Q(1), Q(2), Q(3), Q(4) and Q(5) groups was 1.60%(290/18 168), 1.36%(258/18 970), 1.80%(245/13 583), 1.76%(400/22 739) and 2.35%(362/15 419),respectively (P<0.01) . The person-year incidence of heart failure in Q(1), Q(2), Q(3), Q(4) and Q(5) groups was 1.7, 1.5, 1.9, 1.9 and 2.6 per 1 000 person-years respectively. Compared with the Q(2) group, multivariate analysis with adjustment for major traditional cardiovascular risk factors showed that HRs of Q(3),Q(4),and Q(5) group were 1.23 (95%CI 1.03-1.48, P<0.05) , 1.19 (95%CI 1.01-1.41, P<0.05) , 1.39 (95%CI 1.18-1.65, P<0.01) , respectively. In the absence of hypertension, diabetes, smoking and acute myocardial infarction, the Cox regression model showed that compared with Q(2) group, the HR of new-onset heart failure in Q(5) group was 1.58 (95%CI 1.02-2.45, P<0.05) . Conclusion: Increased RHR is associated with increased risk of new-onset heart failure in this cohort.
Adult
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Blood Pressure
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Cohort Studies
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Female
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Heart Failure
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Heart Rate
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Humans
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Male
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Middle Aged
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Prospective Studies
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Risk Factors
9.Pleiotropic effects of herbs characterized with blood-activating and stasis-resolving functions on angiogenesis.
Li TAO ; Sheng WANG ; Yang ZHAO ; Ai-Yun WANG ; Lei ZHANG ; Jun-Shan RUAN ; Fang-Tian FAN ; Yu-Ping LIU ; Yao LI ; Zhi-Qiang YUE ; Wen-Hui QIAN ; Wen-Xing CHEN ; Yin LU
Chinese journal of integrative medicine 2016;22(10):795-800
Accumulative evidences have underpinned the nature candidates from Chinese medicine (CM), particularly CM served as blood activating and stasis resolving (BASR, Huoxue Huayu in Chinese) by targeting tumor-associated angiogenesis. However, recent experiment research on the therapeutic angiogenesis by BASR-CM attracts wide attention and discussion. This opinion review focused on the underlying link between two indications and anticipated that (1) BASR-CM might emphasize on a balanced multi-cytokines network interaction; (2) BASR-CM might address on the nature of diseases prior to differently affecting physiological and pathological angiogenesis; (3) BASR-CM might mainly act on perivascular cells, either promotes arteriogenesis by increasing arteriogenic factors in ischemic diseases, or simultaneously keep a quiescent vasculature to impede angiogenesis in tumor context.
Angiogenesis Inhibitors
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chemistry
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therapeutic use
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Animals
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Antineoplastic Agents
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therapeutic use
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Drugs, Chinese Herbal
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chemistry
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therapeutic use
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Humans
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Models, Biological
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Neovascularization, Pathologic
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blood
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drug therapy
10.Establish and optimization of real-time fluorescent reverse transcription loop-mediated isothermal amplification for detection of avian influenza H5 hemagglutinin gene.
Yun LIU ; Jin-ming TANG ; Hong TAO ; Jie SUN ; Ti-kang LU ; Li-shan LIAO ; Jian-li LIU ; Shao-ling ZENG ; Chen-fu CAO ; Cai-hong ZHANG ; Zhou-xi RUAN ; Jian-qiang LV ; Jun-xing YANG ; Qun-yi HUA ; Zheng-li CHEN ; Zhi-feng QIN
Chinese Journal of Virology 2013;29(5):488-494
H5 subtype avian influenza (AIV-H5) is a major causative agent of animalloimia a rapid and sensitive molecular biological diagnosis is crucial to the control program of AIV-H5. AIV-H5 real-time fluorescent reverse transcription loop-mediated isothermal amplification (qRT-LAMP) was established by means of heat treatment of the samples. The sensitivity, specificity and repeatability of this method were assessed and the performance of Calcein,SYBR Green I,HNB,SYTO 81 in colorimetric detection was comparatively analyzed to screen the optimum dye. The results showed the sensitivity of this method was 100 times higher than that of standard real-time fluorescent RT-PCR, and the detection limit was one copy of the gene per reaction. This method had no cross-reactivity with other common avian respiratory tract infectious disease-related pathogens such as IBV and NDV. The present study suggested Calcein was the optimum dye. Small-scale tests suggested this method was reliable for survey monitoring of AIV-H5 on the spot, indicating its potential applications in field investigation.
Animals
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Chickens
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Hemagglutinin Glycoproteins, Influenza Virus
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genetics
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Influenza A Virus, H5N1 Subtype
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genetics
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isolation & purification
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Influenza in Birds
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diagnosis
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virology
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Poultry Diseases
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diagnosis
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virology
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Reverse Transcriptase Polymerase Chain Reaction
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instrumentation
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methods
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Sensitivity and Specificity