Objective To establish the quality control standard for Shuxiong soft capsule. Method Radix et Rhizoma Notoginseng, Rhizoma Chuanxiong were identified by TLC, and the content of Ginsenoside Rg1 were detdrmined by HPLC. Result The TLC sports developed was fairly clear. The HPLC method showed good repeatability, and the average recovery of quercitrin was 97.21%, RSD=0.65%. Conclusion The method is simple, accurate and can effectively control the quality of Shuxiong soft capsule.

Purpose:To explore the rational operative approach in patients with carcinoma of the middle third of the esophagus by T and N status as determined by preoperative CT scan. Methods:87 cases with carcinoma of the middle third of the esophagus chosen for the operative approach by CT findings entered the observational group, 118 cases with carcinoma of the middle third of the esophagus who were not scanned by CT were put into the control group. The observational group adopted different operative approaches by the relationship between the primary tumour and the aorta,and the relationship between the primary tumour and the azygos vein,and by the metastasis of regional lymph nodes. The operative approaches were chosen as follows:if the aorta was not involved, the operative approaches were chosen by the metastastatic status of the regional lymph nodes; left thoracoabdominal approaches were chosen,if the aorta was involved,and Ivor-Lewis operation was involved if the azygos vein was involved.The control group adopted the Ivor-Lewis operation. The rate of resection between two groups and the difference in positivity rates of the resected regional lymph node were analysed by chi-square test and T test. Results:Sensitivity of N staging by CT scan was 74.7%(65/87), and specificity of N staging was 60%(39/65). Sensitivity of diagnosis of the aorta involved by tumour by CT scan was 21.8%(19/87), and specificity was (26.3%)(5/19). The rate of resection in the observational group was 92%, and the rate of resection in the control group was'81.4%. Both were markedly different,P

Objective To established a bacteriemia model of BALB/c mice after infection with a ST-239 methicillin-resistant staphylococcus aureus(MRSA) strain, which was isolated and identified from Shanghai Huashan Hospital. Methods We monitored the clinical signs and gross observations of MRSA-infected mice, and examined the histopathology among different groups. Results This isolated MRSA strain ST-239 can induced a typical bacteriemia in BALB/c mice, including the severe mortality and extensive histopathologic injury. However, higher survival rate and slight inflammatory injury were observed in vancomycin-treated mices. Conclusion The solid results obatined in this model will benefit us to study the pathogenic characteristics and patholgenesis in MRSA-induced bacteriemia, and propeled us to seek a safety cure approaches in the future.

Objective To explore the clinical character,diagnosis and treatment principals of pyeloureteritis glandularis and cystitis glandularis.Methods The clinical manifestation,character of imageology and endoscopy images of the case for one year follow-up were analyzed and the relevant literatures were reviewed.Results During one year follow-up,there was no clinical symptoms and any progress on imageology.The patient is still in close following-up.Conclusions According to literatures,cystitis glandularis is not common in clinical,and it should be paid more attention by clinicians.There were a few reports about the ureteritis glandularis.Pelvis,ureter and bladder have the same epithelial tissue,pyelitis glandularis is very rare,up to now there is no efficient treatment ways.

Objective To investigate the functional and structural changes of connectivity of default mode network (DMN) in relapsing-remitting multiple sclerosis (RRMS) by functional MRI (fMRI) and diffusion tensor imaging (DTI) and to study the relationship between them.Methods Twenty-seven RRMS patients(clinically diagnosed as RRMS)and 27 healthy volunteers (matched to the patients in age and gender) were selected to participate in this study.All the subjects underwent 3.0 T MR scanning.Softwares such as DPARSF,MICA,TrackVis were used for data post-processing.Medial prefrontal cortex (mPFC) and posterior cingulate cortex(PCC) of DMN were chosen as ROIs,and functional and structural changes of DMN and the relationship between them were analyzed.Values of r reflecting the functional connectivity and fractional anisotropy(FA) of two groups were obtained.Then statistical analysis was performed by using student t test and Pearson correlation analysis.Results The DMN space pattern of the RRMS patients was mostly identical to that of controls,but with specific difference in the connectivity strength with respect to that of controls.Particularly,the patient group showed increased DMN connectivity in the mPFC,but decreased connectivity in the inferior parietal lobule (IPL) and PCC.Compared with controls,the r value (0.695 ±0.151 vs.0.796±0.085),FA value(0.261 ±0.012 vs.0.285 ±0.017) between mPFC and PCC in RRMS patients were decreased (t =-3.020,-6.206,P ＜ 0.05).In addition,functional connectivity (r value) was found to correlate with structural connectivity(FA value) between the PCC and mPFC (r =0.704,0.735,P ＜ 0.01).The number of MS lesion,had no correlation with r value or FA value (P ＞ 0.05).Conclusions The functional connectivity and structural connectivity of DMN change in RRMS patients.The functional connectivity and structural connectivity between mPFC and PCC are both decreased in RRMS patients compared with the controls.The injury of structural connectivity may be the structural basis of the decrease of functional connectivity.

Objective Adenosine receptor agonist NECA has a certain myocardial protection, but the specific mechanism is not clear.This paper aimed to study the effect and mechanism of NECA inhibiting endoplasmic reticulum stress to against myocardial ischemia reperfusion injury in rats.Methods 56 Wistar rats of SPF grade were selected and divided into Sham group, I/R group, NECA group and TUDCA group through random number table method.We established the isolated rat heart ischemia reperfusion model by using the Langendorff device.Sham group: heart threaded but not ligated, Kerb-Henseleit buffer continuous infusion 170min;I/R group: heart stability 20min, ischemia 30min, reperfusion 2h;NECA group and TUDCA group: heart stability 20min, ischemia 30min, reperfusion 2h, perfusion solutions containing 0.1μmol/L NECA and 30μmol/L TUDCA were respectively given at 5min before reperfusion, and ended at 30min after reperfusion.Transmission electron microscope was used to evaluate alterations of the myocardial ultrastructures.Western blot analysis was used to detected the expression levels of endoplasmic reticulum stress IREl-XBPl signaling pathway marker protein IRE1α, XBP1s.Immunohistochemical staining was used to detect the expression of IRE1α.Results The results of transmission electron microscopy showed that most of the myofilament ruptured, sarcomere contracture deformation, visible mitochondrial vacuoles degeneration in I/R group, and injury in NECA group and TUDCA group were less than the I/R group, appeared as the filaments arranged more neat, sarcoma only had mild contracture.Immunohistochemical results showed that IRE1αpositive staining was not found in the sham group, and the area of positive staining of IRE1α in I/R group was significantly increased, while the NECA group and TUDCA group were significantly decreased.Compared to the Sham group, the expression level of IRE1α and XBP1s was significantly increased in I/R group(P<0.05);but compared with the expression level of IREα and XBP1s in I/R group(1.72±0.27, 0.97±0.19), the NECA group(1.14±0.16, 0.6±0.13) and the TUDCA(1.07±0.27, 0.58±0.15) group were significantly decreased(P<0.05).Conclusion NECA can reduce endoplasmic reticulum stress through inhibiting IREl-XBPl pathway to protect the myocardium.

At present, there is a relative shortage of multimedia classroom resources of medical schools. Yan'an Hospital of Kunming Medical College proposed the new model of teaching methods of portable multimedia classrooms composed of MP5. According to the teaching practice and the results from the questionnaire, it is approved by the teachers and students.

Objective To compare the diagnostic ability of triple-phase CT and multiple-phase dynamic MR for patients with suspected hepatocellular carcinoma (HCC). Methods Triple-phase CT and multiple-phase dynamic MR scan were performed in 60 patients. Fifty-nine HCC lesions were confirmed in 39 patients. MR was performed with LAVA technique, the images included masks, dual-artery phases, dual-portal phases, dual-venous phases and delayed phase. Three observers separately evaluated the CT and MR imaging, and the results were compared with alternative-free-response ROC(AFROC)curve, the area under ROC (Az) was calculated to compare the diagnostic ability. Results The mean Az value of CT for the diagnosis of HCC was 0.8120±0.0118, of MR was 0.9093±0.0072 (P>0.05). In the group of HCC less than 1 cm in the diameter, the sensitivity of CT and MR was 63.89% and 80.55%(P＝0.013). In the groups HCCs of 1-3 cm and >3 cm, the sensitivity of CT and MR appeared no significant difference (P>0.05). Of all HCCs, the sensitivity of CT was lower than MR (83.62% vs 88.70%), but the difference was not significant (P>0.05). The positive predict value (PPV) of CT was also lower than MR (93.07% vs 96.31%, P>0.05). Conclusion The diagnostic ability of multiple-phase dynamic-enhancement MR scan for HCCs is similar to that of triple-phase enhancement CT. For HCC less than 1 cm in diameter, dynamic-enhancement MR is superior to that of contrast-enhancement CT scan, while for the larger ones, the difference is not significant.

Objective To evaluate the detectability of dual-arterial phase of MRI and single-artery phase of CT scan for hepatocellular carcinoma (HCC). Methods A total of 39 patients with HCC underwent CT and MR scan, and 59 lesions of HCC were confirmed definitely. According to lesion size, the lesions were divided into 3 groups: >3 cm group (n=20), 1-3 cm group (n=27) and <1 cm group (n=12). CT was performed with 25 seconds delaying for artery phase. MR imaging was performed with liver accelerate volume acquisition (LAVA) technique, dual-artery phases included early artery phase of 17 seconds delaying and a mid-artery phase of 24 seconds delaying. The detectability of dual-artery phase of MR was compared with that of single-artery phase of CT. Results In <1 cm group, the sensitivity of CT single-artery phase images and MR dual-artery phase images in detecting HCC lesions was 50.00% (6/12) and 75.00% (9/12), respectively;the later showed a higher sensitivity (P=0.04). In groups of 1-3 cm and ＞3 cm, the sensitivity of the two technique had no statistical difference (66.67% vs 81.48% and 95.00% vs 95.00%). Conclusion For the detection of <1 cm HCC, dual-artery phase MRI has higher detectability than single artery phase enhancement CT.