1.Three cases of trigeminal neuralgia caused by basilar artery twist.
Wei-dong QI ; Ming LI ; Xing-qiang GAO ; Zhao-xin MA
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(3):219-220
Aged
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Aged, 80 and over
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Basilar Artery
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abnormalities
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Female
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Humans
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Male
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Middle Aged
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Trigeminal Neuralgia
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etiology
2.Expression and clinical significance of osteopotin in calcified breast tissue
Jinliang HUAN ; Qiang WANG ; Li XING ; Xianju QIN ; Wangkou MA ; Yubing PENG ; Zhiguang GAO
Chinese Journal of General Surgery 1997;0(04):-
Objective To investigate the expression of osteopontin(OPN) mRNA in breast tissues containing microcalcifications and the significance of OPN in tumor pathogenesis and metastasis of breast cancer.Methods The expression of OPNmRNA in 128 samples of tissues of breast lesions and adjacent breast tissues and 9 samples of metastatic lymph nodes were examined.Results The expression of OPNmRNA was highest in(calcified) foci of breast cancer tissues and in metastatic lynph nodes,lower in calcified foci of benign breast tissues and lowest in breast tissues adjacent to breast cancer and in benign tissues without calcification.The differences between the defferent tissues were significant(all P
4.The Application of Heating Effect in Breeding of Microorganism
Xing-Qiang GAO ; Yun-Hong HUANG ; Fei DAI ; Xue-Qin FU ; Zhong-Er LONG ;
Microbiology 2008;0(10):-
Advances in mechanism and application of the heating effect in breeding of microorganism are reviewed in this paper. Heat produces mutagenesis effect and screening effect. Heating mutagenesis effect is occurred through the substitution of G-C base pair induced by heat, and heating screening effect produces higher forward mutation rate induced by other mutagens.
5.A phytochemical and pharmacological advance on medicinal plant Litsea cubeba (Lauraceae).
Shui-Ying ZHANG ; Qiang GUO ; Xiao-Li GAO ; Zhi-Qin GUO ; Yun-Fang ZHAO ; Xing-Yun CHAI ; Peng-Fei TU
China Journal of Chinese Materia Medica 2014;39(5):769-776
Litsea cubeba is one of aromatic medicinal plant belonging to family Lauraceae. The roots, stems and fruits of L. cubeba have been widely applied as folk medicines in some districts in China for relieving rheumatism and cold, regulating Qi (meridian) to alleviate pain. Previous studies revealed that this species contains major alkaloids, in specific aporphines, and minor flavonoids, lignans as well. Related pharmacological investigations demonstrated its activities and clinical applications on cardiovascular diseases, anti-cancer, against rheumatoid arthritis, relieving asthma and anti-allergic effects, as anti-oxidants, and so on. As an effort for further exploration of this bioactive ingredients and potential drug development, this paper summarizes most phytochemical and pharmacological results. Further, future prospects are also included.
Animals
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Drug Therapy
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Humans
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Litsea
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chemistry
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Molecular Structure
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Plant Extracts
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chemistry
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pharmacology
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Plants, Medicinal
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chemistry
6.Alkaloids from roots and stems of Litsea cubeba.
Shui-Ying ZHANG ; Qiang GUO ; Yuan CAO ; Yuan ZHANG ; Xiao-Li GAO ; Peng-Fei TU ; Xing-Yun CHAI
China Journal of Chinese Materia Medica 2014;39(20):3964-3968
A phytochemical investigation on the roots and stems of Litsea cubeba led to the isolation of seven isoquinolone alkaloids. By spectroscopic analysis and comparison of their 1H and 13C-NMR data with those in literatures, these alkaloids were identified as (+)-norboldine (1), (+)-boldine (2), (+)-reticuline (3), (+)-laurotetanine (4), (+)-isoboldine (5), (+)-N-methyl-laurotetanine (6), and berberine (7), respectively. Among them, 7 was isolated from the genus for the first time. The evaluation of these compounds showed weak anti-inflammatory activity against NO production in RAW 267.4 and BV-2 cells.
Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Litsea
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chemistry
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Molecular Structure
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Plant Roots
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chemistry
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Plant Stems
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chemistry
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Spectrometry, Mass, Electrospray Ionization
7.Consistency analysis of Keratograph and traditional methods to evaluate tear film function
Pei-Yang, SHEN ; Hai-Bo, CHEN ; Hong-Shan, LIU ; Ming-Bing, ZENG ; Xiong-Gao, HUANG ; Jian-Qiang, XING ; Xing-Wu, ZHONG
International Eye Science 2015;(5):846-849
?AlM: To investigate repeatability and accuracy of a latest Keratograph for evaluating the tear film stability and to compare its measurements with that of traditional examination methods.
?METHODS: The results of noninvasive tear film break-up time ( Nl-BUT ) including the first tear film break-up time ( BUT-f ) and the average tear film break-up time ( BUT - ave ) were measured by Keratograph. The repeatability of the measurements was evaluated by coefficient of variation ( CV ) and intraclass correlation coefficient ( lCC) . Wilcoxon Signed-Rank test was used to compare Nl-BUT with fluorescein tear film break-up time ( FBUT) to confirm the correlation between Nl-BUT and FBUT, Schirmer l test values. Bland-Altman analysis was used to evaluate consistency.
?RESULTS: The study recruited 48 subjects ( 48 eyes ) (mean age 38. 7±15. 2 years). The CV and lCC of BUT-f were respectively 12. 6% and 0. 95, those of BUT-ave were 9. 8% and 0. 96. The value of BUT-f was lower than that of FBUT. The difference had statistical significance ( 6. 16±2. 46s vs 7. 46±1. 92s, P<0. 01). There was significant positive correlation between Nl-BUT and FBUT, Schirmer l test values ( P< 0. 01 ). The scope of 95% limits of agreement (LoA) was 4. 46s in BUT-f and FBUT, while the scope of LoA was 3. 64s in BUT-ave and FBUT.
?CONCLUSlON: Keratograph can provide Nl-BUT data that has a better repeatability and reliability, which has great application prospects in diagnosis and treatment of dry eye and refractive corneal surgery.
8.Saikosaponin-b regulates the proliferation and apoptosis of HepG2 cells by targeting the MACC1/c-Met/Akt signaling pathway
LYU XING-ZHI ; LI RUI-FANG ; GAO ZI-HAN ; WANG HONG-WEI ; LI SANG-QIANG ; WANG JIAN-GANG
Chinese Journal of Pharmacology and Toxicology 2017;31(10):965-966
OBJECTIVE Metastasis-associated in colon cancer-1 (MACC1) is an oncogene that has been newly identified. It promotes tumor proliferation and invasion via the MET pathway. Our study investigated the effects of Saikosaponin-b(SS-b) on the proliferation and apoptosis of HepG2 cells and its regulation on MACC1/c-Met/Akt signaling pathway. METHODS HepG2 cells were treated with SS-b (10-800 g·L-1) for 48 h in vitro. The CCK-8 assay was used to assess cell proliferation, and cell apoptosis was determined by Hoechst33258 staining, AnnexinⅤ/PI staining and caspase 3 assay. RT-PCR was used to examine the expression of MACC1, c- MET and hepatocyte growth factor (HGF) mRNA. MACC1 protein was detected by Western blot and immunohistochemistry. The protein expressions of p-c-MET, c-MET, p-AKT, AKT, p-BAD, BAD were measured by Western blot. RESULTS SS-b inhibited the growth of HepG2 cells in dose-dependent way and induced cell apoptosis significantly. HepG2 cells showed karyopyknosis, fragmentation and fluorescence highlight in SS-b treatment group. FCM results showed that apoptosis rate of HepG2 cells increased with SS- b concentration. The immunofluores?cence results showed that the MACC1 expression decreased significantly in HepG2 cells treated with SS-b. The expression levels of MACC1, c-MET and HGF mRNA in HepG2 cells were significantly inhibited by SS-b. SS-b also significantly decreased the protein expressions of MACC1, p-c-MET and p-AKT while increased the expression of p-BAD and caspase 3 in HepG2 cells(P<0.05). CONCLUSION SS-b inhibited the proliferation and induced the apoptosis of HepG2 cells by targeting the MACC1/c-Met/Akt signaling pathway.
9.Surgical treatment of trigeminal neuralgia with microvascular decompression sensory root of trigeminal nerve combing.
Wei-dong QI ; Xing-qiang GAO ; Ming LI ; Jian-ning ZHANG ; Yi CAO ; Zhao-xin MA
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2007;42(10):731-734
OBJECTIVETo evaluate the short-term and long-term curative effect of microvascular decompression sensory root of trigeminal nerve combing (MVD + SRTNC) by post-sigmoid approach as well as the complications in these operations.
METHODSThe data from 138 idiopathic trigeminal neuralgia (TN) patients treated with MVD + STRNC that followed up 33 months to 58 months was analyzed.
RESULTSImmediate relief from pain occurred in 136 patients (98.6%). On Kaplan-Meier analysis, cumulative proportion effectiveness at the end of 25 months decreased to 91.4%, and 85.8% from 49 months to 58 months. There were cases of 63.83% and 19.15% with slight and midrange hypoesthesia after surgery but majority recovered at the end of 3-58 months (P < 0.01). No serious hypoesthesia occurred. The complications including leakage of cerebrospinal fluid, herpes and headache caused by high intracranial pressure, were observed in 4 cases (2.90%), 36 cases (26.09%) and 3 cases (2.17%), respectively. Two cases (1.43%) died of cerebral hemorrhage.
CONCLUSIONSThe proposed surgical strategy of standard MVD plus sensory root of trigeminal nerve combing was a good strategy option for TN, but It was important for surgeons to think highly of the risks of the surgery.
Adult ; Aged ; Aged, 80 and over ; Decompression, Surgical ; Female ; Humans ; Male ; Microcirculation ; Middle Aged ; Treatment Outcome ; Trigeminal Nerve ; blood supply ; surgery ; Trigeminal Neuralgia ; surgery ; Young Adult
10.A study on eating disorders among overweight and obese adolescents in Bengbu
HUANG Qing, FANG Li, GAO Xing, ZHANG Qiang, HAN Hui
Chinese Journal of School Health 2019;40(7):1021-1023
Objective:
To investigate the status of eating disorders among overweight and obese adolescents as well as provide the basis for developing obesity precautionary and eating disorders.
Methods:
910 adolescents of grade 4 to 6 in primary schools and grade 1 to 3 in junior middle schools were recruited in Bengbu by cluster random sampling to examine the association between obesity and eating disorders.
Results:
A total of 203 children (22.3%) were overweight and obese, 547 children (60.1%) were normal weight and 160 children (17.6%) were low weight. The prevalence of overweight and obese in boys were significantly higher than those in girls (30.6% vs. 13.0%; χ2=40.55, P<0.01). Compared with normal weight group and low weight group, the scores of drive for thinness (DT), body dissatisfaction (BD), low self-esteem (LSE) and eating disorder risk composite (EDRC) were significantly higher in overweight and obese group (P<0.05). There was a positive association between the scores of DT(r=0.19), BD(r=0.30), LSE(r=0.09), interceptive deficits (ID) (r=0.08) and EDRC (rs=0.11) with body mass index (BMI)(P<0.05).
Conclusion
Eating disorders were associated with overweight and obesity of adolescents. A targeted strategies and measures should be conducted.