OBJECTIVE:To establish the quality standard of Tangkang capsule. METHODS:Momordica charantia L. and Euonymus alatus(Thunb.)Sieb.,the main ingredients in Tangkang capsule including were identified by TLC, meanwhile, the content of total Momordica charantian saponin was determined by UV-Vis. RESULTS: The TLC spots were clear and the separation degree was good. The linear range of Ginsenoside Rg1 was 72～252 ?g(r=0.999 7) with average recovery rate at 100.68% (RSD=1.43%,n=6). CONCLUSION: The established quality standard can be used for the quality control of this Tangkang capsule.

OBJECTIVE:To compare the clinical efficacy and safety of rivaroxaban and low molecular weight heparin calcium (LMWHA)in the prevention of deep venous thrombosis(DVT)after total hip arthroplasty(THA). METHODS:A total of 100 THA patients selected from orthopedics department of our hospital as research objects were divided into control group and observa-tion group according to random number table,with 50 cases in each group. Control group was treated with LMWHA injection 0.4 mL subcutaneously,qd;observation group was given Rivaroxaban tablet 10 mg orally,qd. Both groups received treatment on the first day after surgery,for consecutive 14 d. Coagulation indexes(PT,APTT,Fib,TT,D-D),VAS score,the incidence of DVT and PE were observed in 2 groups. The postoperative bleeding volume and ADR as hematoma and gastrointestinal bleeding were compared between 2 groups. RESULTS:Before treatment,there was no statistical significance in coagulation indexes or VAS scores between 2 groups(P>0.05). After treatment,PT,APTT,TT and D-D levels,VAS scores of 2 groups were decreased sig-nificantly,while Fib levels were increased significantly;VAS score of observation group was significantly lower than that of con-trol group,with statistical significance(P<0.05). There was no statistical significance in coagulation indexes between 2 groups (P>0.05). The incidence of DVT and PE in observation group were 8.00% and 7.50% ,which were significantly lower than 12.00% and 4.00% of control group,with no statistical significance(P>0.05). The postoperative bleeding volume of observation group was(298.31±52.18)mL,which was significantly lower than(327.40±54.20)mL of control group,with statistical signifi-cance(P<0.05). There was no statistical significance in the incidence of hematoma or gastrointestinal bleeding between 2 groups (P>0.05). CONCLUSIONS:Rivaroxaban and LMWHA can significantly improve coagulation state,prevent the generation of DVT after THA. While rivaroxaban is better in shortening pain time without increasing the risk of ADR.

It is discussed in this paper the origins and pathogenesis of treating senile disease from spleen in TCM,and the relationship between various methods of invigorating the spleen and senile deficiency syndromes.It has been proposed that the therapy principles of invigorating qi and spleen,stimulating the appetite to awake the splee,primering blood to go back to the spleen,dehumidification to activate spleen-energy,warming the kidney and spleen,dispersing the depressed liver-energy to belifit the spleen,going on dieting to belifit the spleen and clearing away heart-fire to support spleen etc.are used to prevent and cure senile diseases.It also listed the corresponding treatment prescriptions for the elderly patients.It provides a theoretical basis for treatment of senile diseases from the governance of spleen.And it provided a realistic basis and the implementation of the method for the prevention and treatment of elderly patient's deficiency aging.

Objective To study the effect of extracorporeal shock wave therapy with percutaneous autogenous bone marrow grafting in the mannagement of long bone nonunion.Methods After success in marrow aspiration from patients' iliac crest,19 cases of patients accepted the treatment of bone marrow grafting and ESWT.Then,it's therapeutic effect was compared with 23 cases of single ESWT group,such as average rate of bone union and average healing time during follow-up period.The data of this study was analyzed by independent-samples t test,and a P value of

Objective:To discuss the result of treating valgus or varus knee deformity with fixator assist nailing (FAN)technique and distal femur osteotomy(DFO).Methods:In this retrospective study,14 patients with 17 limbs,12 valgus and 5 varus knee deformity were treated with DFO and FAN.The aver-age age of the patients was 23 (17-44)years .The unilateral external fixator was mounted on the lateral side of femur,the minimal invasive distal femoral osteotomy was performed.After the deformity was cor-rected,the intramedully nail was inserted to fix the femur.None of the patients need bone autograft.The preoperation and postoperation lateral distal femur angle (LDFA ),mechanical axis deviation (MAD ) and range of motion (ROM)were measured and analyzed.Results:All the 14 patients were followed-up for 12 -72 months (average 34 months).The osteotomy site united in 3 -5 months (average 3.5 months)post-operatively.According to Paley’s functional scores,12 patients were excellent,2 patients were good.We achieved desired postoperative MAD (from medial 15 mm to lateral 10 mm)in 15 limbs of 12 patients.The LDFA in 1 1 limbs was corrected to normal (84°-90°).The ROM was not signifi-cantly changed before and after operation in the both groups.No infection or neuro-vascular injury oc-curred.Conclusion:The FAN technique is an effective method to treat valgus knee or varus knee de-formity in young patients with DFO.

Objective To explore the application value of thromboelastography(TEG)in perioperative evaluation of benign pros-tatic hyperplasia(BPH) patients treated with anticoagulants .Methods To retrospective analyze the intraoperative bleeding ,postop-erative bleeding and transfusion treatment of 120 patients with BPH surgery ,who were treated with anticoagulants .The results of TEG detection and coagulation function test were compared .Results There was no patients with significant intraoperative bleed-ing ,including 40 cases of bleeding after surgery ,80 cases of patients with no bleeding .In patients of bleeding ,there were 32 benign prostatic hyperplasia patients(80 .00% )with low coagulation function detected by TEG detection ,and 18(45 .00% ) with low coagu-lation function detected by routine coagulation function test .The difference of detection rates was statistical significant between the two methods(χ2 =12 .709 ,P=0 .002) .In patients of non-bleeding ,there were 76 benign prostatic hyperplasia patients(95 .00% ) with non-low coagulation function detected by TEG detection ,and 67(83 .75% )with non-low coagulation function detected by rou-tine coagulation function test .The difference in detection rate was significant between the two methods(χ2 =6 .679 ,P=0 .022) . Conclusion TEG could effectively evaluated postoperative bleeding in BPH patients ,guide postoperative transfusion therapy ,which is better than routine coagulation function test .

Objective To investigate the susceptibility ofepidermal cells to ultraviolet A(UVA)-induced apoptosis in dopachrome tautomerase knockout Dct~(-/-) mice versus wildtype C57BL/6J mice.Methods High titer of anti-Ro/SSA-positive sera collected from three patients with SLE and typical cutaneous phntosensitivity were intraperitoneally injected into both Dct~(-/-) and wildtype mice,which were then chronically exposed to UVA irradiation at a single dose of 10 J/cm~2 three times a week for two weeks.Then,UVA-irradiated tail skin was excised from each mouse,embedded with paraffin,cut into 4 to 5-μm sections followed by hematoxylin/eosin staining and terminal deoxynucleotidyl transferase nick end labeling(TUNEL),respectively,for the counting of sunburn cells(SBC) and apoptotie cells.Results After chronic UVA exposure,the number of SBC and TUNEL-positive cells per 100 epithelial cells was significantly higher in serum-injected Dct~(-/-) mice than in serum-injected wildtype mice(14±1.0 vs 7±-0.6,62±2.7 vs 30 ±1.6,both P<0.05).A significant decrease was also observed in the number of SBC (6 ±0.9 per 1 00 epithelial cells)and TUNEL-positive cells (42±2.5 per 100 epithelial cells)in uninjected Dct~(-/-) mice compared with those of serum-injcoted Dct~(-/-) mice(both P<0.05).Conclusions The deficiency of Dct gene increases the susceptibility of epidermal cells to UVA-induced apoptosis under the presence of anti-Ro/SSA antibody,which potentially contributes to the develop-ment of anti-Ro/SSA antibody-mediated photosensitivity in SLE.

Caenorhabditis elegans (C.elegans), has developed into an useful model for biomedical research, particularly in the targets of novel drugs and the related mechanism.C.elegans, combining with cost-effective cultivation, simple biological structure, make for high-throughput screenings in vivo model.In this review, we explore the feasibility of C.elegans in drug activity evaluation of complex systems.Because of the body independence of C.elegans, it provided good platform for the efficacy and mechanism of research on new drugs as well as Chinese herbal extract in vivo, showing a good prospect.

Objective To analyze the clinical value of X ray examination, CT examination and MRI examination in the diagnosis of giant cell tumor of bone.Methods 50 patients with giant cell tumor of bone confirmed by postoperative pathology were selected.The patients' X ray, CT scan and MRI imaging examination data were retrospectively analyzed, and the imaging features of giant cell tumor of bone were discussed and summarized.Results The rate of CT examination and MRI examination in the diagnosis of bone destruction was higher than X ray examination, but the difference was not statistically significant (x2=4.181,P>0.05).CT examination and MRI examination in the soap bubble sign, bone crest, liquid plane and soft tissue mass in the diagnostic rate compared with X ray examination was significantly higher, the difference was statistically significant (x2=5.333,22.941,30.000,38.400,64.000,14.166,6.102,all P<0.05).Conclusion The diagnostic accuracy of CT and MRI in giant cell tumor of bone is higher than X ray, but the value of MRI examination in the diagnosis of edema near the tumor is better.