1.Progress in the research of insulin-like growth factor family in lung cancer
Liangkun YOU ; Yongde LIAO ; Shengling FU ; Sheng JU ; Guang CHEN ; Xin XING
Tumor 2010;(4):356-360
Insulin-like growth factors (IGF), regulated by their receptors and binding proteins, play a pivotal role in human cell proliferation, differentiation and apoptosis. Increasing evidence has revealed that IGF system is involved in the genesis and progress of various malignancies including lung cancer. Recent studies in regard to IGF axis expression in the lung cancer cell lines, pulmonary tissue samples and blood circulation of lung cancer patients have shown that the IGF axis may contribute to the transformation and progression of lung cancer. Several researches have shown that a number of drugs targeting the IGF receptor are being investigated in clinical trials and suggest a potential therapeutic efficacy. This article reviews the updates and progress in the research of IGF axis in lung cancer.
2.Effects of Qinggan Huoxue Recipe and its separated recipes on urokinase-type plasminogen activator and plasminogen activator inhibitor-1 fibrinolytic system in rats with alcoholic liver fibrosis.
Junming CHEN ; Lei WANG ; Tao LIU ; Lianjun XING ; Peiyong ZHENG ; Guang JI
Journal of Integrative Medicine 2009;7(7):642-50
To study the action mechanisms of Qinggan Huoxue Recipe (QGHXR), a compound traditional Chinese herbal medicine, and its separated recipes by observing their effects on expressions of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) in rats with alcoholic liver fibrosis (ALF).
3.Three Cases of Drug-drug Interaction Intervened by Therapeutic Drug Monitoring for Voriconazole
Jingjie YU ; Sitao YANH ; Xing ZHOU ; Guiying CHEN ; Guang DU ; Dong LIU ; Yang ZHANG
Herald of Medicine 2017;36(8):879-883
Objective To discuss the role of therapeutic drug monitoring (TDM) in pharmaceutical care by successful intervention of severe drug-drug interaction in 3 patients with hematological disease treated with voriconazole and rifampin.Methods Three patients with hematological disease were monitored for the plasma concentration of voriconazole before,during,and after the concomitant use of rifampin.The severity of this drug interaction was revealed,risks for developing invasive fungal infection and tuberculosis dissemination after chemotherapy were evaluated based on the TDM results,and alternative regimens were recommended.Results Voriconazole plasma concentration was normal at baseline but significantly depressed after combination with rifampin in all 3 cases.Concomnitant use of rifampin leads to a rapid decline in plasma concentration of voriconazole in 2-3 days,and withdraw of this enzyme induction effect takes 8-10 days after discontinuation of rifampin.Conclusion TDM is a helpful tool for providing pharmaceutical care,it helps to objectively visualize the degree of clinically important drug-drug interactions.Clinical evidence together with TDM results suggests high risk for developing invasive fungal infection and tuberculosis dissemination in hematology patients while using this combination therapy.Discontinuation of rifampin was suggested and accepted.For these patients,combination of voriconazole and rifampin should be avoided.
4.Application of serious game learning method in Chinese medical history
Youliang HUANG ; Renquan LIU ; Shuangqing ZHAI ; Guang CHEN ; Qiongjie YAO ; Xing ZHAI
Chinese Journal of Medical Education Research 2014;(9):909-911
“Mechanical memory” approach was commonly used in the knowledge learning of traditional Chinese medicine, which makes students often feel that the knowledge is too abstract, and this method is thus ineffective. “Qihuang the way” using Unity 3D game development platform inte-grates the elements of medical knowledge of the Chinese medical history, and combines TCM theoretical knowledge learning with serious games , which can help invoke students' enthusiasm and improve learning efficiency in an entertaining way.
5.Sampling survey of 0—6 years old disabled children in Guiyang
Shi-xing LUO ; Zu-ming LI ; Guang-yong CHEN ; Ying CHEN ; Guilan CAI ; Ziyun LIU ; Guirong ZHANG ; Runshu LUO
Chinese Journal of Rehabilitation Theory and Practice 2004;10(6):340-342
ObjectiveTo survey 0—6 years old disabled children in Guiyang.Methods4 999 children aged 0—6 years old chosen randomly from 9 145 children of 12 blocks were investigated with the disability sampling survey criteria of 1987.ResultsThe rate of disabled children in Guiyang was 1.58%, and was not significantly different compared with that of whole country (1.36%) and other three areas including Siping City (P>0.05),but significantly higher than that of other six districts including Heping district of Tianjin municipality (P<0.05). All kinds of disability found by this survey were separately intellectual disability (0.92%),comprehensive disability (0.28%),limb disability (0.24%),vision disability (0.10%),psychiatric disability (0.03%),and hearing disability (0.02%). Rates of intellectual and vision disabilities were significantly higher than that of whole country ( P>0.05).ConclusionSome diseases are directly relative to occurrence of disability. The poor income of the children family,lower education of his parents and not gotten preschool education were risk factors of disability. The institution based rehabilitation and community based rehabilitation are main rehabilitation forms to disabled children.
6.Chemical constituents of the root of Wrightia pubescens R.Br.
Xing-Xiang CHEN ; Guang-Jie ZHANG ; Hui-Min CUI ; Li CHEN ; Shi-Jun LIU ; Jun-Xing DONG
Journal of International Pharmaceutical Research 2017;44(12):1137-1140,1149
Objective To investigate the chemical constituents of the root of Wrightia pubescens.Methods Compounds were isolated by the combined use of colum chromatography,preparative HPLC and recrystallization,and their structures were identified by their physicochemical and spectroscopic data.Cytotoxic activities of the compounds were evaluated using MTT method in vitro.Re?sults Twelve compounds 1-12 were isolated from the ethyl acetate soluble part from 75% ethanol extract of the root of W.pubescens, and identified as scopoletin(1),coumarin(2),cleomiscosin B(3),mollugin(4),4-hydroxybenzoic acid(5),vanillic acid(6), vanillin(7),4-hydroxymethyl-5-hydroxy-2H-pyran-2-one(8),β-sitosterol(9),β-daucosterol(10),ursonic acid(11),and me?dioresinol(12). Compound 4 showed cytotoxic activity against HepG2 cells in vitro with IC50of 8.0 mg/L. The IC50of compound 11 against MCF-7 and HepG2 cells was 14.7 and 18.2 mg/L,respectively.Conclusion Compounds 1-5,8 and 12 were isolated from the genus Wrightia for the first time. Compounds 6 and 10 were isolated from the title plant for the first time. Compounds 4 and 11 showed cytotoxic activities against tumor cells in vitro.
7.Effects of Wuling mycelia on pentylenetetrazole-induced epilepsy in rats.
Guan-feng CHEN ; Guang-li REN ; Li-san ZHANG ; Xing-yue HU
Journal of Zhejiang University. Medical sciences 2012;41(6):647-652
OBJECTIVETo investigate the effects and mechanisms of Wuling mycelia on seizure development and learning ability induced by pentylenetetrazole-kindling epilepsy in rats.
METHODSSD rats were randomly divided into four groups: pentylenetetrazole-kindling model group (model group), low dose Wuling mycelia (0.3 g*kg(-1)) group (LD-WM group), high dose Wuling mycelia (0.6 g*kg(-1)) group (HD-WM group) and control group. The rats were intraperitoneal injected with a subconvulsive dose (35 mg*kg(-1)) of pentylenetetrazole (saline in control group) every 48 h for 12 times. Wuling mycelia was intragastrically applied 30 min before pentylenetetrazole injection. An 8-arm radial maze ( 4 arms baited) was used to measure the learning ability. Histamine was measured by chemical fluorometric enzyme immunoassay.
RESULTSCompared with the model group, the kindling stage of LD-WM group degraded significantly after 7th injection, the latency to the onset of myoclonic jerks (LTMJ) and the latency to the onset of generalized seizures (LTGS) prolonged after the 6th and 7th injection, respectively (P<0.05). The kindling stage of HD-WM group also degraded markedly after the 6th to 8th injection, and the LTMJ and the LTGS extended after the 8th to 9th and 6th injection, respectively (P<0.05). Compared with the control group, the frequency of working memory error (WME) and reference memory error (RME) of the model group in the 8-arm radial maze increased through 3-d training (P<0.05). The memory tests showed that the impairment induced by pentylenetetrazole was partially reversed by Wuling mycelia. Compared with the control group, brain histamine contents (hippocampus, cortex, thalamus and hypothalamus) were significantly lower in model group (P<0.05). But compared with the model group, hippocampal histamine contents in LD-WM group and hippocampal, thalamic and hypothalamic histamine contents in HD-WM group were elevated (P<0.05).
CONCLUSIONWuling mycelia can delay the kindling and ameliorate the ability of learning in rats with pentylenetetrazole-induced epilepsy and the enhancement of neuronal histamine activity may be one of possible mechanisms.
Animals ; Disease Models, Animal ; Drugs, Chinese Herbal ; therapeutic use ; Epilepsy ; chemically induced ; metabolism ; prevention & control ; Hippocampus ; metabolism ; Histamine ; metabolism ; Kindling, Neurologic ; drug effects ; Learning ; drug effects ; Male ; Pentylenetetrazole ; toxicity ; Rats ; Rats, Sprague-Dawley
8.Apoptosis-related gene expression and its clinical significance of human osteosarcoma.
Xing WU ; Zheng-rong CHEN ; Guang-jian ZHANG
Chinese Journal of Oncology 2004;26(11):678-681
OBJECTIVETo explore the prognostic markers in osteosarcoma.
METHODSExpressions of p53, c-myc, bcl-2 and apoptosis index (AI) in 28 osteosarcoma specimens were detected by ABC immunohistochemistry and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labelling (TUNEL). The relationship between gene expression and apoptosis, and their correlations with pathologic classification and prognostic factors were analyzed.
RESULTSThere was negative correlation between the expressions of p53, c-myc, bcl-2 protein and AI, which was closely related to the long term survival of patients but was not related to pathologic types of the tumor.
CONCLUSIONThe expressions of p53, c-myc, bcl-2 protein and AI can be used as an index for predicting the progression and prognosis of osteosarcoma.
Adolescent ; Adult ; Apoptosis ; Biomarkers, Tumor ; Bone Neoplasms ; metabolism ; pathology ; Disease Progression ; Female ; Follow-Up Studies ; Humans ; Male ; Osteosarcoma ; metabolism ; pathology ; Prognosis ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Survival Rate ; Tumor Suppressor Protein p53 ; metabolism ; bcl-2-Associated X Protein
9.Current status and prospect of translational medicine in nanotechnology.
Guang-yu GAO ; Mei-ling CHEN ; Ming-yuan LI ; Zhen-bo YANG ; Zhi-ping LI ; Xing-guo MEI
Acta Pharmaceutica Sinica 2015;50(8):919-924
Nowadays, nanotechnologies have shown wide application foreground in the biomedical field of medicine laboratory tests, drug delivery, gene therapy and bioremediation. However, in recent years, nanomaterials have been labeled poisonous, because of the disputes and misunderstandings of mainstream views on their safety. Besides, for the barriers of technical issues in preparation like: (1) low efficacy (poor PK & PD and low drug loading), (2) high cost (irreproducibility and difficulty in scale up), little of that research has been successfully translated into commercial products. Currently, along with the new theory of "physical damage is the origin of nanotoxicity", biodegradability and biocompatibility of nanomaterials are listed as the basic principle of safe application of nanomaterials. Combining scientific design based on molecular level with precision control of process engineering will provide a new strategy to overcome the core technical challenges. New turning point of translational medicine in nanotechnology may emerge.
Biocompatible Materials
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Nanostructures
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toxicity
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Nanotechnology
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Translational Medical Research
10.Hypereosinophilic syndrome in a 2 month-old infant.
Xiao-ran AN ; Xing-guo WANG ; Jing-hua CHEN ; Xiao-feng YUAN ; Li CONG ; Ji-fang WANG ; Guang-shen LI
Chinese Journal of Pediatrics 2003;41(5):378-378
Anemia
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etiology
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Erythema
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etiology
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Fatal Outcome
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Female
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Fever
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etiology
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Humans
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Hypereosinophilic Syndrome
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complications
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diagnosis
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Infant