1.Study on indications regularity of analogous decoctions of Erchen Decoction
Shuhui HUANG ; Weiwei ZENG ; Bin XING
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
To explore indications regularity of analogous decoctions of Erchen Decoction.323 analogous decoctions of Erchen Decoction were found in Concise Thesaurus of TCM Formula.Diseases and symptoms of TCM were analyzed.22 diseases and 118 symptoms of TCM were found.The main indication of analogous decoctions of Erchen Decoction included spleen-stomach and lung system,other symptoms were broadly involved.Erchen Decoction was the most appropriate for phlegm.
2.Changes of brain - derived neurotrophic factor expression in gene modified bone marrow mesenchymal stem cells
Mao-Song, XIE ; Guo-Xing, XU ; Li-Bin, HUANG
International Eye Science 2016;16(10):1816-1819
AIM: To study the changes of brain - derived neurotrophic factor ( BDNF ) expression in gene modified bone marrow mesenchymal stem cells ( BMSC) .
●METHODS:BMSC were divided into blank control group ( without transfected BMSC ) , negative control group ( empty vector without BDNF gene transfected BMSC) and experimental group ( BDNF gene transfected BMSC) . The expression of BDNF mRNA in BMSC was measured by Realtime PCR, and the expression of BDNF in BMSC was measured by ELlSA.
●RESULTS:The BDNF mRNA expressions of 3, 4, 5, 6, 7 and 8-generation BMSC cells in the experimental group were higher than those in the blank control group and negative control group. The differences were statistically significant (P3: F=491. 788, P<0. 05; P4: F=380. 112, P<0. 05;P5:F=1854. 929, P<0. 05; P6: F=224. 540, P<0. 05;P7:F=619. 155, P<0. 05; P8: F=10. 092, P<0. 05). As the BMSC cells in the experimental group passaging, the BDNF mRNA expressions in the experimental group decreased. The difference of BDNF mRNA expression among different passage cells was statistically significant (F=298. 603, P<0. 05). The BDNF secretion of 3, 4, 5, 6, 7 and 8-generation BMSC cells in the experimental group were higher than those in the blank control group and negative control group. The differences were statistically significant (P3: F=520. 609, P<0. 05; P4: F=734. 520, P<0. 05;P5:F=152. 847, P<0. 05;P6:F=80. 372, P<0. 05; P7:F=96. 083, P<0. 05;P8:F=38. 532, P<0. 05). As the BMSC cells in the experimental group passaging, the BDNF secretion decreased. The difference of BDNF secretion among different passage cells was statistically significant (F=230. 084, P<0. 05).
●CONCLUSION:Long-term expression of BDNF in BMSC can be enhanced by genetic engineering.
3.Relationship between serum uric acid and carotid atherosclerosis in male patients with type 2 diabetes
Qian HUANG ; Yao XU ; Bin GAO ; Pangmin LI ; Jie MING ; Ying XING ; Qiuhe JI
Chinese Journal of Endocrinology and Metabolism 2011;27(7):566-569
Objective To investigate the relationship between the level of serum uric acid(SUA) and carotid atherosclerosis in male patients with type 2 diabetes mellitus(T2DM). Methods A collection of 579 male T2DM patients with or without carotid atherosclerosis were grouped based on quartiles of SUA. Age, SUA, smokers, duration, body mass index(BMI), blood pressure, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), blood urea nitrogen(BUN), creatinine(Cr), and HbA1C were determined in all subjects. The plaques in carotid arteries and intima-media thickness(IMT) were measured with high-resolution ultrasound. Results BMI, systolic and diastolic blood pressures, TG, and Cr showed a gradual increase, while HDL-C and HbA1C showed a gradual decrease according to the higher SUA quartiles in male T2DM(P<0.05). Nevertheless, the detectable rates of smokers, duration, age, TC, LDL-C, and BUN had no relationships with the SUA quartiles(P>0.05). The detectable rate of carotid atherosclerosis and the thickness of carotid plaque were positively associated with the levels of SUA in male patients with T2DM(P<0.05). However, intima-media thickness of carotid arteries did not illustrate the correlation with the levels of SUA in male T2DM patients(P>0.05). Age, HbA1C, and SUA were independent factors of carotid atherosclerosis in these patients by logistic regression(P<0.05). Conclusion The levels of SUA seems to be associated with the occurrence and development of carotid atherosclerosis in male patients with T2DM.
4.Protective effect of asiatic acid from Potentilla chinensis on alcohol hepatic injury in rats.
Gan ZHAO ; Shu-juan LV ; Gang WEI ; Jin-bin WEI ; Xing LIN ; Quan-fang HUANG
China Journal of Chinese Materia Medica 2015;40(14):2866-2870
To study the protective effect and the mechanism of asiatic acid (AA) from Potentilla chinensis on alcohol hepatic injury in rats. Male Wistar rats were randomly divided into six groups: the normal control group, the AA control group (8 mg · kg(-1) AA), the model group (5.0-9.0 g · kg(-1) alcohol) and high, medium and low-dose AA-treated groups (alcohol + 8, 4, 2 mg · kg(-1) AA). Each group was orally administered with the corresponding drugs once a day for 24 weeks. Approximately 1. 5 hours after the final administration, all rats were killed, and their blood samples and hepatic tissues were collected. The AST and ALT in rat serum and the contents of MPO, TNF-α, IL-1β, SOD, GSH-Px, GSH-Rd and MDA in hepatic tissues were detected. The expressions of NF-κB, TLR4, CD14, MyD88, TRIF and protein expression in hepatic tissues were measured by western blot. The pathological changes in liver tissues were observed by histological examination. The results showed that compared with the model group, the AA-treated groups showed significant decreases in serum ALT, AST and MDA and increases in the activities of SOD, GSH-Px, GSH-Rd and MPO. Moreover, AA markedly inhibited the expressions of TNF-α, IL-1β, TLR4, CD14, MyD88 and NF-κB. The histological examination showed alleviated hepatic issue ijury to varying degrees. In short, asiatic acid (AA) from P. chinensis could protect alcohol-induced hepatic injury in rats. Its mechanism may be related to the inhibition of NF-κB inactivation and the reduction of inflammatory response.
Animals
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Liver
;
drug effects
;
pathology
;
Liver Diseases, Alcoholic
;
prevention & control
;
Male
;
NF-kappa B
;
physiology
;
Pentacyclic Triterpenes
;
pharmacology
;
Potentilla
;
chemistry
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Protective Agents
;
pharmacology
;
Rats
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Rats, Wistar
;
Toll-Like Receptor 4
;
antagonists & inhibitors
5.Evaluation of therapy effect of 131Ⅰ treatment of nasopharyngeal carcinoma xenograft mediated by hNIS gene transfection in vivo using diffusion weighted MR
Xing ZHONG ; Changzheng SHI ; Jian GONG ; Bin GUO ; Li HUANG ; Hao XU
Chinese Journal of Radiology 2012;46(10):937-942
ObjectiveTo investigate the imaging characteristic of DW1,tumor cell apoptosis and proliferation of nasopharyngeal carcinoma (NPC) xenograft transfected hNIS gene after 131Ⅰ treatment.MethodsThe NPC xenograft models were established with CNE-2-hNIS cells(experimental group) and CNE-2 cells(control group) respectively.After i.p.injections 131Ⅰ in the experimental group and control group,the changes of xenograft tumor volume and ADC value were observed in 3,6,12,18,24 days by MR scan.The correlations of changes of ADC with pathological TUNEL,Caspase-3 immunohistochemistry of apoptosis,and Ki-67 proliferation detection were further investigated.Independent samples t-test were compared between the two groups and Pearson linear correlation analysis was used.ResultsThe tumor volume of the experimental group was significantly reduced compared with that of the control group after 131 Ⅰ treatment (t values:8.27-19.46,P <0.05 ).DW-MRI showed that in the 3th day after 131Ⅰ treatment,ADC values increased in the experimental group,and ADC values reached the peak in the 6th and 12th day after 131Ⅰ treatment,then ADC values were decreased. ADC values after treatment was positively correlated with apoptotic index ( r =0.72,P < 0.05 ) and caspase-3 positive rate ( r =0.65,P < 0.05) and there was a negative correlation with Ki-67 proliferation index ( r =- 0.71,P < 0.05 ).ConclusionADC values can reflect growth inhibition of NPC xenograft transfected hNIS gene after 131 I treatment.It is possible that DWMRI techniques can be used in early non-invasively monitor the therapy effect of 131Ⅰ treatment of NPC xenografts transfected hNIS gene.
6.Bartter's syndrome:report of 12 cases
Xing-Duan HUANG ; Bin YAO ; Zhi-Min HUANG ; Zhi-Hong LIAO ; Jian-Ping WENG ; Guo-Liang HU ; Yan-Bing LI ;
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Twelve cases of Bartter's syndrome were reported and reviewed retrospectively.Usually vomiting was the first sympton in children,while fatigue was common in adults.Bartter's syndrome was characteristic of hypokalemia,metabolic alkalosis,elevations of plasma renin activity,serum angiotersinⅡand aldosterone and juxtaglomerular apperatus hyperplasia.Supplementation of potassium choloride was the main manner of therapy.
7.Analysis on body impairment assessment upon 447 thoracolumbar, spinal injury cases in traffic accidents.
Sheng-yan LI ; Si-xing HUANG ; Xing-yao SHAN ; Bin KONG ; Kuan-lin LIU
Journal of Forensic Medicine 2006;22(1):61-64
OBJECTIVE:
To discuss the relation between degree of body impairment and that of thoracolumbar spinal injuries resulting from road traffic accidents, and sum up the experiences in body impairment assessment and its regularity.
METHODS:
For comprehensive body impairment assessment, 477 cases of thoracolumbar spinal injuries in road accidents have been sorted out, reassessed and rediagnosed. In addition, analyses have been undertaken about their treatment, the assessment of the degree of their thoracolumbar dysfunction,nerve dysfunction and the relations between injuries and sequelaes.
RESULTS:
The analyses show that the degree of thoracolumbar dysfunction and that of the post-injury nerve dysfunction don't necessarily depend on the quantity and degree of spinal injuries. However, the position suffering from the thoracolumbar spinal injuries has an immense impact on the thoracolumbar dysfunction, and the nerve impairment result mainly from the T1-1L spinal injuries. The research also shows that there has been a high misdiagnosis rate in hospital about the spinal injuries.
CONCLUSION
In body impairment assessment, the cause and effect relations between the injury and degree of injury extent should be analyzed, the injury extent should be employed as principal evidence, and the degree of spinal dysfunction should be taken into greater consideration.
Accidents, Traffic
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Adolescent
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Adult
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Age Distribution
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Aged
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Aged, 80 and over
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Disability Evaluation
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Female
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Humans
;
Injury Severity Score
;
Lumbar Vertebrae/physiology*
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Male
;
Middle Aged
;
Nervous System/physiopathology*
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Retrospective Studies
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Spinal Injuries/physiopathology*
;
Thoracic Vertebrae/physiology*
;
Young Adult
8.Forensic identification of floating shoulder injury.
Sheng-Ya LI ; Si-Xing HUANG ; Xing-Bin ZHAO ; Xiao-Hong ZHENG ; Yi ZHU
Journal of Forensic Medicine 2006;22(5):355-358
OBJECTIVE:
To discuss forensic identification of floating shoulder injury (FSI).
METHODS:
To analyze fifteen cases of FSI which were accepted from Jan. 1993 to Jan. 2006, including 15 shoulder neck fracture, 13 clavide stem fracture and 2 distal end clavide fracture, the function of shoulder joint was evaluated six months after injure considering the following three aspects: result of forensic examination such as X-ray photograph, CT and MRI, the injurers' symptom, objective sign and joint function, shoulder joint territory, degree of pain and local muscle power.
RESULTS:
Basing on the curative effect standard of Herscovic, all cases were divided into good. Modest, worst, which included 2, 4, 9 cases respectively; referring the standard of GA35-92, GB18667-2002, all cases were divided into six, seven, eight, nine and ten degree, which included 2,9,2,1,1 cases respectively.
CONCLUSION
As a special powerful injure, FSI always companied with concurrent and multiple injure, and characterized by missed, incorrect and delayed diagnosis and infelicitous treatment, which lead to the high frequency and degree of injure. To prevent missed and incorrect forensic identification, we should have a full realization of the particularity of FSI, and evaluate the function of shoulder all-sidely, objectively and synseticaly.
Accidents, Traffic
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Adolescent
;
Adult
;
Clavicle/surgery*
;
Female
;
Forensic Dentistry/methods*
;
Fractures, Bone/surgery*
;
Humans
;
Male
;
Middle Aged
;
Pain/pathology*
;
Range of Motion, Articular
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Retrospective Studies
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Scapula/surgery*
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Shoulder Injuries
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Shoulder Joint/physiopathology*
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Tomography, X-Ray Computed
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Wounds and Injuries/surgery*
;
Young Adult
9.Forensic application of brainstem auditory evoked potential in patients with brain concussion.
Xing-Bin ZHENG ; Sheng-Yan LI ; Si-Xing HUANG ; Ke-Xin MA
Journal of Forensic Medicine 2008;24(6):433-441
OBJECTIVE:
To investigate changes of brainstem auditory evoked potential (BAEP) in patients with brain concussion.
METHODS:
Nineteen patients with brain concussion were studied with BAEP examination. The data was compared to the healthy persons reported in literatures.
RESULTS:
The abnormal rate of BAEP for patients with brain concussion was 89.5%. There was a statistically significant difference between the abnormal rate of patients and that of healthy persons (P<0.05). The abnormal rate of BAEP in the brainstem pathway for patients with brain concussion was 73.7%, indicating dysfunction of the brainstem in those patients.
CONCLUSION
BAEP might be helpful in forensic diagnosis of brain concussion.
Accidents, Traffic
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Adolescent
;
Adult
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Brain Concussion/physiopathology*
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Child
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Evoked Potentials, Auditory, Brain Stem
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Female
;
Forensic Medicine
;
Humans
;
Male
;
Middle Aged
;
Young Adult
10.Growth hormone enhances regeneration of nNOS-containing nerve fibers in the aged rat penis.
Xing HUANG ; Li-Quan HU ; Bin-Qun TIAN
National Journal of Andrology 2005;11(2):98-100
OBJECTIVETo investigate the effects of growth hormone (GH) on the erectile function and the number of neuronal nitric oxide synthase (nNOS) -containing nerve fibers in the penis of aged rats.
METHODSTwenty-four aged male SD rats (18 months) were randomized into 2 groups: GH intervention group and control group. After four and eight weeks, a half of each group were selected and tested for erectile function after apomorphine (APO) injection and then sacrificed for the detection of nNOS-containing nerve fibers in the penis by streptavidin-peroxidase conjugated method (SP method).
RESULTSAfter four weeks, the erectile function and the number of nNOS-containing nerve fibers showed no significant difference between the GH intervention group and the control group (P > 0.05). After eight weeks, the erection frequency was significantly different (P < 0.05) between the two groups, while the erection rate was not. The number of nNOS-containing nerve fibers in the GH intervention group was significantly larger than that in the control group (P < 0.01).
CONCLUSIONGH enhances the regeneration of nNOS-containing nerve fibers in the penis and improves the erectile function of the aged rat.
Animals ; Apomorphine ; pharmacology ; Human Growth Hormone ; pharmacology ; Immunohistochemistry ; Male ; Nerve Fibers ; enzymology ; physiology ; Nerve Regeneration ; drug effects ; Nitric Oxide Synthase Type I ; analysis ; Penile Erection ; drug effects ; Penis ; enzymology ; innervation ; Random Allocation ; Rats ; Rats, Sprague-Dawley