OBJECTIVE To strengthen occupational safety and health(OSH) management of medical service. METHODS To analyze actuality of OSH management and internal and international laws and regulations for OSH management,and give the proposition of its construction. RESULTS On the basis of the requirement,a set of OSH management scheme was established. CONCLUSIONS It is imperative that construct occupational safety and health management laws and regulations and system in medical service.They are pacing factors for physical and mental health of medical members.

OBJECTIVE To select applicable air disinfectors that can be used at indoor dynamic state to disinfect air in hospital.METHODS Some different air disinfectors were installed different volumetric rooms.To detect indoor air bacterial content before and after running the air disinfectors at quiet state and dynamic state.To evaluate the disinfection effect of these air disinfectors according to the eliminateion rates.RESULTS At quiet state,the indoor bacteria eliminateion rate exceed 90% after the air disinfectors running 0.5h.So the disinfection were qualified.At dynamic state,these air disinfectors showed good lasting disinfection effect.CONCLUSIONS The air disinfectors solve the problem of indoor air disinfection at dynamic state.It is imperative that select and use exactly the air(disinfectors) to get good disinfection effect.

OBJECTIVE To give experimental basis for further investigation on Staphylococcus epidermidis biofilm resistance,we investigated the erythromycin repression of the planktonic,resuspended and biofilm cells.METHODS log Reduction of the planktonic,resuspended and biofilm cells of S.epidermidis strain 1457 and wild isolate S 68 was tested under 100 MIC erythromycin.The cells inside biofilm were observed by transmission electron microscope.RESULTS log Reduction of the planktonic and resuspended cells of S.epidermidis 1457 were 5.56?0.11 and 5.28?0.08,respectively,after 4h under 100 MIC erythromycin,which was higher than that of biofilm cells(P0.05).The tendency of the log reduction of isolate S 68 was the same as that of strain 1457.The cells of biofilm without erythromycin were spherical and of even distributed,but the swelling,liquefied cells and cell debris were observed in the biofilm with erythromycin after 12h.The arrangement of cells inside biofilm with erythromycin was looser than that without erythromycin.CONCLUSIONS The erythromycin resistance of biofilm cells is higher.Biofilm can give protection for the bacteria to avoid the killing of action by antibiotics.

OBJECTIVE To confirm the clinical disinfection effect of acidity electrolyzed oxidizing water(AEOW).METHODS According to the standards of disinfection techniques supplied by Health Ministry of China,the disinfection effect of health care worker,object surfaces and medical apparatus and instrument were investigated.RESULTS The log reduction of the bacteria on the hands,object surfaces and medical instruments were more than 1.00.The number of the bacteria on the endoscopes were 0-2 CFU per piece on average.No pathogenic bacteria were detected out.The disinfection effect of each experimental group was qualified.CONCLUSIONS The field disinfection effect of AEOW is accepted.

The traditional Chinese medicine (Tripterygium wilfordiiHook.f.,TWH) has been clinically used to treat primary and secondary renal diseases and proteinuria for nearly 40 years.However,there is a rare literature about the effect of triptolide (the main active ingredient of TWH) on the expression of oxidative carbonyl protein (OCP) in diabetic nephropathy (DN).This study aimed to provide experimental evidence for triptolide treatment on DN through its effect on the expression of OCP,in order to investigate the effects of triptolide on the expression of OCP in rats with DN.Sixty SD rats were randomly divided into five groups:control group,high-dose triptolide (Th) group,low-dose triptolide (T1) group,DN model group,and positive control (benazepril) group.The DN model was established using streptozotocin.Urinary protein excretion,fasting blood glucose (FBG),superoxide dismutase (SOD) in renal homogenate,malondialdehyde (MDA) in renal homogenate and renal nitrotyrosine by immunohistochemistry,and the expression of OCP by oxyblotimmune blotting were detected.In the DN model group,rat urinary protein excretion and renal MDA were significantly increased,while renal SOD significantly decreased and nitrotyrosine expression was obviously upregulated in the kidney.After triptolide treatment,24-h urinary protein excretion (61.96±19.00 vs.18.32±4.78 mg/day,P＜0.001),renal MDA (8.09±0.79 vs.5.45±0.68 nmol/L,P＜0.001),and nitrotyrosine expression were decreased.Furthermore,renal OCP significantly decreased,while renal SOD (82.50±19.10 vs.124.00±20.52 U/L,P＜0.001) was elevated.This study revealed that triptolide can down-regulate the expression of OCP in the renal cortex of DN rats.

Bacterial disinfectant resistance is the phenomenon that minimal inhibitory concentration or minimal bactericidal concentration of a certain disinfectant increases after a certain bacterium contacts with it many times. It exists widespread. Many species of bacteria are may resistant to a certain disinfectant, and a species of bacterium is may resistant to many disinfectant Disinfectant selectivity pressure is the extrinsic agent of bacterial disinfectant resistance. Resistance mechanisms include bacterial biochemistry structure, genetics pathway and enzymology pathway. There is relationship in disinfectant resistance and drug resistance. We should strengthen study and monitoring, enact unified standard and application specification to reduce bacterial disinfectant resistance.

The effects of surfactants on the production of cellulase by Trichoderma viride in liquid substrate fermentation process were investigated. Straw was used as the sole carbon source and the surfactants were biosurfactant rhamnolipid from Pseudomonas aeruginosa and Tween 80. The changes of FPA,CMCase,Avicelase and surface tension with time were analyzed under different concentrations of the two surfactants. The results showed that the surfactants can enhance the enzyme activity of Trichoderma viride. The FPA,CMCase,Avicelase were promoted 1.08,1.6 and 1.03 times higher than the controls by rhamnolipid. The enhancement of the enzyme activity by rhamnolipid was much higher than that of Tween 80. At the same time,rhamnolipid was not degraded prior to other substrate.

Objective:To explore the clinical features and prognosis of urachal carcinoma.Methods:The clinical data of 35 patients with urachal carcinoma admitted to the First Affiliated Hospital of Zhengzhou University from August 2011 to November 2019 were analyzed retrospectively. There were 23 males and 12 females, with a male to female ratio of 1.92∶1. The average age was (52.1±13.9) years old, and the median age was 55 years old. There were 8 patients with a history of smoking and 3 patients with a history of drinking. There were 5 cases of hypertension, 5 cases of diabetes, 2 cases of coronary heart disease, and 1 case of cerebral infarction. The first symptoms were hematuria in 25 cases, lower abdominal pain in 4 cases, abdominal mass in 2 cases, umbilical discharge in 1 case, and asymptomatic in 4 cases.Preoperative CT examination showed that the tumor was located on the top wall of the bladder in 24 cases and the front wall of the bladder in 11 cases. There were 25 solid tumors and 10 cystic tumors. The maximum diameter of the tumor was 1.5 to 11.0 cm, and the median maximum diameter of the tumor was 4.0 cm. Preoperative cystoscopy detected masses on the anterior or top wall of the bladder and urachal carcinoma was suspected in 35 cases. All 35 cases underwent enlarged partial cystectomy (conventional resection of the umbilical part), and 3 cases underwent pelvic lymph node dissection at the same time. Among them, 19 cases underwent open surgery, 14 cases underwent laparoscopic surgery, and 2 cases underwent Da Vinci robot assisted laparoscopic surgery.Results:According to Mayo staging, there were 10 cases of stage Ⅰ, 18 cases of stage Ⅱ, 1 case of stage Ⅲ, and 6 cases of stage Ⅳ. The overall follow-up rate was 91.4% (32/35), and the median follow-up time was 41 (3-103) months. The 1-year survival rate was 82.5%, the 3-year survival rate was 59.3%, and the 5-year survival rate was 53.9%. Univariate analysis showed that age ( P=0.033), maximum tumor diameter ( P=0.011), lymph node metastasis ( P=0.002), distant metastasis ( P=0.011), pathological grade ( P=0.001), Mayo staging ( P=0.026) were ralated prognostic factors (all P<0.05). Cox multivariate analysis showed that the pathologically poor differentiation ( HR=1.640, 95% CI 1.112-2.418, P=0.013), and the largest tumor diameter ≥4cm were ( HR=5.000, 95% CI 1.099-22.755, P=0.037). Independent factors affecting patient prognosis. Conclusions:Urachal carcinoma is a malignant bladder tumor with insidient onset. Most of the first diagnosis symptoms are hematuria. When diagnosed, most patients are in the middle and late stages of clinical grading, and the prognosis is poor.Pathological grade and maximum tumor diameter are independent factors that affect the prognosis of patients with urachal carcinoma. The higher was the pathological grade, and the larger was the maximum tumor diameter, the worse was the prognosis.

The effect of magnetic Fe3O4 particles on cellulase in the enzymatic hydrolysis of sunflower seed hull was studied in different adding ways and additive amount. In the process of enzymatic hydrolysis of sunflower seed hull, the variations of cellulase activity, reducing sugar concentration and cellulose conversion were evaluated. After the reaction, the analysis of pH and surface tension of hydrolysate were also used to determine the mechanisms of cellulase by the magnetic effect. The results indicated that after adding magnetic Fe3O4, the cellulase activity, reducing sugar concentration and conversion of cellulose had an increased between the 0.5 g/L and 2.0 g/L cases after 48 h. When the additive amount of magnetic Fe3O4 was 2 g/L, the cellulase activity at 60 h was improved significantly by 25.9%. It was found that the concentration of reducing sugar was increased from 6.950 mg/mL to 8.775 mg/mL with magnetic Fe3O4 1.5 g/L. Simultaneously, compared with the blank, which the conversion of cellulose was 47.932%, the maximum celluloseconversion of samples with adding magnetic Fe3O4 was 60.531%. Besides, the stability of cellulase activity adding in times was better than in one time. After the reaction, the final surface tension of hydrolysate with 1.5 g/L magnetic Fe3O4 was the lowest in comparison with the blank. However, no significant differences were observed in the final pH of the hydrolysate.

OBJECTIVE To probe into the new method to improve medical environment,and reduce hospital infection causing by the environmental factors.METHODS Using the nanometer photocatalysis technology clinically,to analyze the bacteriostatic effect of medical environment.RESULTS After use of the nanometer photocatalysis spraying material the surface sterilization effect was obvious on the three kinds of environmental objects.The withering away rate of bacterium on the object with total surface was 23.75-38.30%;the vertical surface bacterium withers away rate was 48.77-87.97%,the horizontal surface bacterium withers away rate was 12.50-77.20%,the air bacterium withering away rate was 1.52-60.00%.CONCLUSIONS The nanometer photocatalysis technology to sterilize and purify the medical environment has very good perspectives.