Objective To assess the efficacy of the laryngeal mask airway i-gel (LMA i-gel) in patients undergoing neuro-interventional surgery. Methods Forty ASA Ⅰ or Ⅱ patients of both sexes, aged 20-60 yr,body mass index ＜ 30 kg/m2, undergoing elective neuro-interventional surgery, were randomized into 2 groups (n = 20 each):LMA i-gel group (group I) and LMA ProSeal group (group P). After induction of anesthesia with TCI of propofol and remifentanil, LMA i-gel and LMA ProSeal were inserted in group I and P respectively. The patients were mechanically ventilated and PETCO2 was maintented at 35-40 mm Hg,SpO2 at 99%-100%. BP and HR were monitored and recorded before induction, immediately after induction, at 1, 3 and 5 min after insertion of LMA and immediately after removal of LMA. The success rate, LMA placement time, leak pressure, peak airway pressure and complications were recorded. Results There was no significant difference in the success rate, the LMA platcement time and peak airway pressure between the two groups (P ＞ 0.05). BP and HR were within the normal range during operation in both groups. The leak pressure and incidence of blood stain and sore throat were significantly lower in group I than in group P (P ＜ 0.05). Conclusion LMA i-gel can provide adequate ventilation during operation with less complications and can be used effectively for neuro-interventional surgery.

Chinese materia medica (CMM) has double complexity in bioactive ingredient and its mechanism. It is difficult to explain by the modern biomedicine theory So it seriously restricts the modernization of CMM The modern CMM should have the high quality standard to meet the needs of international standard It can be guaranteed by spreading the GAP for Chinese medicinal materials and GMP for standard production The mechanism depends on using the DNA microarray to set up “the gene expression difference chart”, to study on the combination of CMM and gene expression difference chart Meanwhile, we can establish a totally new method of screening modern CMM based on the gene expression difference chart, it can really make the modernization and internationalization of CMM

OBJECTIVE: To examine AMCase mRNA expression levels in nasal mucosa of allergic rhinitis SD rats. METHOD: Thirty SD rats were chosen and randomly divided into the experimental group and the control group. 20 in experimental group and 10 in the control group. AR model rats were established through repeated intraperitoneal shot of ovalbumin (OVA) for 2 weeks and consequently confirmed by local challenge with OVA for 1 week. The control group was treated by the same method with Physiological saline water instead of OVA. After the last excitation allergic rhinitis was diagnosed according to the accumulation score about nasal symptom. The septal mucosa of all rats were used to diagnose pathologically by HE dyeing. AMCase mRNA in nasal mucosa, obtained from the bilateral nasal mucosa in two groups, were used to do reverse transcriptive polymerase chain reaction. Real-time polymerase chain reaction was used to examine AMCase mRNA expression levels. RESULT: (1) The results showed definitely that there were positive expression of AMCase mRNA in normal nasal mucosa. This expression increase significantly during nasal allergy (P < 0.05). (2) The increased expression of AMCase mRNA in allergic rhinitis are related with the nasal symptoms score (r = 0.411, P < 0.05). CONCLUSION Increased expression of AMCase mRNA in nasal mucosa in allergic rhinitis model might play roles in the pathogenesis of AR, and Restrain the enzyme activity could become new treatment targets of allergic rhinitis.
Animals ; Chitinases ; metabolism ; Nasal Mucosa ; metabolism ; Ovalbumin ; RNA, Messenger ; metabolism ; Rats ; Rats, Sprague-Dawley ; Rhinitis, Allergic ; enzymology ; metabolism

Objective To evaluate the applicability of formula R0, R1, R2 of ECToolbox software in measurement of left ventricular ejection fraction (LVEF) with gated myocardial imaging. Methods Rest gated myocardial SPECT with ~(99m)Tc-MIBI and rest gated blood-pool SPECT with ~(99m)Tc-RBC were performed on 64 patients (44 with CHD, 20 with hypertension). LVEFs were separately calculated with formula R0, R1, R2 of ECToolbox software, and the results were recorded as R0 LVEF, R1 LVEF and R2 LVEF, respectively, and compared with LVEFs from gated blood-pool imaging (GBPI LVEF). Results The LVEFs from formula R0, R1 and R2 were all significantly correlated with GBPI LVEF (r=0.905, 0.905 and 0.903, P<0.05, respectively). The accuracy within ±15% deviation from the GBPI LVEF was 54.30%, 71.40% and 22.90%, and within ±30% was 81.40%, 88.60%,74.30%, respectively. The differences among R0 LVEF, R2 LVEF and GBPI LVEF were statistically significant (P<0.05), while between R1 LVEF and GBPI LVEF was not (P>0.05). Conclusion The formula R1 of ECToolbox software is most applicable in measuring LVEF with gated myocardial imaging.

Aim To investigate the effects of ginaton on tubular cell apoptosis in a rat model of renal ischemia/reperfusion(I/R) injury.Methods The model of bilateral post-ischemic renal injury was established by clamping renal pedicles for 45 minutes.To explore the effects of ginaton on activation and expression of JNK and c-Jun,p-JNK1/2,JNK,p-c-Jun,c-Jun were examined by Western blotting.Apoptosis of renal tubular epithelial cells was assessed by terminal deoxynucleotidyl transferase mediated dUTPnick end labeling(TUNEL) and flow cytometric analysis.Results Phosphorylation levels of the JNK and the c-Jun were significantly increased at 20 min after renal I/R injury.This corresponds to a significant increase of apoptotic tubular cells induced by renal I/R injury.Ginaton attenuated the increased phosphorylation of p-JUK at 20 min(vs I/R 20 min,P

Objective To investigate the significance of EGF in the in vitro maturation, fertilization, and pre-implantation development of the immature oocytes. Methods The immature oocytes were isolated from the ovaries of the rats. The germinal vesicle (GV) oocytes were cultured in the EGF solutions at concentrations of 0(control), 0.5, 1, 2.5, 5 and 10 ng/ml Ham’s F10 culture media. The status of germinal vesicle break-down(GVBD) and the extrusion of the first polar body (PB1) were observed. The achieved MⅡoocytes were then inseminated in vitro; and the pronuclei (PN) and the pre-implantaon development were observed. The gradeⅠ-Ⅱ4-cell embryos were selected and implanted into the fallopian tube of the rats with the 1 day pseudogestation. Follow-up observations were made till the delivery. Results After GV oocytes were treated with EGF solutions at concentration of 1, 2.5, 5 and 10 ng/ml for 12, 16, 20, and 24 h, the GVBD in the GV cumulus-oocyte complexs(COC) have better outcomes than that of the control group(P

Objective:To study the expression of aromatasecy P450 and receptor of estrogen and progesterone in the tissue of endometrial hyperplasia and endometrial cancinoma,analysis the correlation of the endometrial carcinoma pathogenesis,clinical stage and cell differentiation.Methods:The immunohistochemiscal S-P method techniques were performed to study the expression of aromatasecy P450,estrogen receptor(ER) and progesterone receptor(PR) in the tissue of endometrium from 226 cases,including 42 cases of normal endometrium,52 cases of simple endometrial hyperplasia,38 cases of complex endometrial hyperplasia,36 cases of endometrial atypical hyperplasia and 58 cases of endometrial carcinoma.Results:There was no the positive expression in the normal endometrial tissues.But the positive expressions of aromatasecy P450 were found in the tissue of endometrial hyperplasia(simple endometrial hyperplasia,complex endometrial,atypical hyperplasia)and endometrial carcinoma.The rates of the positive expression were 40.4%,47.4%,69.4%,72.4% respectively.There were significant relationships among fifth groups(P

ObjectiveTo investigate the effects of the asphyxia as a Second Hit on renal function during early stage after birth in small for gestational age (SGA) infants.MethodsThe infants who were hospitalized within 24 hours after birth in Peking University Third Hospital between January 2013 and March 2015 were retrospectively enrolled, and divided into different groups depending on gestational age and asphyxia history. There were 40 preterm non-asphyxia SGA infants and 80 controls who were preterm non-asphyxia appropriate for gestational age (AGA) infants; 11 preterm asphyxia SGA infants and an equal number of preterm asphyxia AGA infants as controls; 33 term non-asphyxia SGA infants and 33 term non-asphyxia AGA infants as controls; and four term asphyxia SGA infants and 13 term asphyxia AGA infants as controls. Blood urea nitrogen (BUN), serum creatinine (SCr), and estimate glomerular filtration rate (eGFR) were tested within 48 h after admission and the incidence of abnormal indexes was compared between groups byt-test and Fisher exact test.Results(1) Compared with preterm non-asphyxia AGA group, BUN level significantly decreased in preterm non-asphyxia SGA group [(3.99±1.69) vs (5.11±2.08) mmol/L,t=2.948, P=0.004]. Compared with term non-asphyxia AGA group, term non-asphyxia SGA group had higher SCr level [(72.03±10.29) vs (62.58±12.27)μmol/L,t=3.390,P=0.001] and lower eGFR level [(25.19±4.07) vs (33.99±8.75) ml/(min·1.73 m2),t=5.238,P=0.000]. (2) Compared with preterm non-asphyxia AGA infants, preterm asphyxia AGA infants had higher BUN [(6.96±3.09) vs (5.11±2.08) mmol/L,t=2.602,P=0.011] and SCr [(76.45±10.11) vs (66.70±13.18)μmol/L,t=2.357,P=0.021] and lower eGFR level [(15.86±2.31) vs (19.54±5.08) ml/(min·1.73 m2),t=2.361,P=0.020]. Compared with preterm non-asphyxia SGA group, there was a significant increase in BUN level [(6.70±3.37) vs (3.99±1.69) mmol/L,t=2.581,P=0.025] and decrease in eGFR level [(14.80±4.67) vs (18.66±5.03) ml/(min·1.73 m2),t=2.285,P=0.027] in preterm asphyxia SGA group. Changes in term infants were similar to preterm infants. (3) Compared with asphyxia AGA group, asphyxia SGA group showed a higher frequency of abnormal eGFR in term infants (4/4 vs 4/13, Fisher exact test,P=0.029). ConclusionsAsphyxia as a probable Second Hit can influence the renal function during early stage in both preterm and term infants, especially in SGA infants.