The traditional method of separation and screening is complicated,time-consuming,poor selectivity,as well as that the active ingredient is easy to lose,seriously restricting the rapid discovery of active ingredients from Chinese herbal medicine.With the development of analytical techniques,various chromatographic methods were used for screening the active compounds from complex mixtures,this article will summarize the recent advances in the chromatographic methods used for tyrosine inhibitors screening from traditional Chinese medicines.

Objective To establish a HPLC-MS/MS method for simultaneous determination of the concentration of simvastatin, simvastatin acid and rivaroxaban in rats plasma. Methods The plasma samples were determined after secondary extraction with ethyl acetate. Ticagrelor and lovastatin were used as internal standards. Simvastatin, simvastatin acid and rivaroxaban in rats plasma were separated by Waters C18 (150. 0 mm × 4. 6 mm, 3. 5 μm) with mobile phase consisting of acetonitrile?ammonium (75: 25, pH = 4. 5) . Electrospray ionization source was applied and operated in positive multiple reaction monitoring mode. Polarlity switch (negative-positive mode) was performed. The detector was operated in multiple reaction-monitoring mode at m/z 436. 0→144. 9 for simvastatin, m/z 452. 9 → 162. 3 for simvastatin acid, m/z 324. 1 →127. 2 for rivaroxaban, m/z 521. 1→361. 2 for Ticagrelor and m/z 405. 3→199. 0 for lovastatin. The specificity, standard curve and lower limit ofquantification, precision and recovery, stability and matrix effect of the method were investigated. Results The linear ranges of simvastatin, simvastatin acid and rivastatin in plasma were 1-100, 5-400 and 1-100 ng·m L-1, standard curve were y = 0. 78 x + 7. 00 × 10-4 (r = 0. 992 8) , y = 0. 59 x + 2. 57 × 10-2 (r = 0. 997 2) and y = 1. 09 x + 1. 79 ×10-2 (r = 0. 996 4) , and the lower limit of quantitation were 1, 5 and 1 ng·m L-1, respectively. The intra-day and inter-day standard deviation were less than 15％, and the recovery rate reached more than 70％. The plasma samples had a good stability with freezing-thawing for three times, -80 ℃ for 5 days and 4 ℃ for 24 h. And there was no obvious matrix effect. Conclusion The method was simple, specific and sensitive, suitable to detect a number of samples for pharmacokinetic study.

After one-month of oral treatment with traditional Chinese medicine decoction, without using other drugs, the lung inflammatory exudate, pulmonary fibrosis and quality of life of a 61-year-old female patient with corona virus disease 2019 (COVID-19) were significantly improved. No recurrence or deterioration of the patient's condition was found within seven weeks of treatment and follow-up, and no adverse events occurred, indicating that oral Chinese medicine decoction was able to improve the pulmonary inflammation and fibrosis in a patient recovering from COVID-19, but further research is still needed.
Administration, Oral ; COVID-19/virology* ; Drugs, Chinese Herbal/therapeutic use* ; Exudates and Transudates ; Female ; Humans ; Inflammation/etiology* ; Lung/pathology* ; Magnoliopsida ; Medicine, Chinese Traditional ; Middle Aged ; Phytotherapy ; Pulmonary Fibrosis/etiology* ; SARS-CoV-2

Orthodontically induced tooth root resorption (OIRR) is a serious complication during orthodontic treatment. Stimulating cementum repair is the fundamental approach for the treatment of OIRR. Parathyroid hormone (PTH) might be a potential therapeutic agent for OIRR, but its effects still lack direct evidence, and the underlying mechanisms remain unclear. This study aims to explore the potential involvement of long noncoding RNAs (lncRNAs) in mediating the anabolic effects of intermittent PTH and contributing to cementum repair, as identifying lncRNA-disease associations can provide valuable insights for disease diagnosis and treatment. Here, we showed that intermittent PTH regulates cell proliferation and mineralization in immortalized murine cementoblast OCCM-30 via the regulation of the Wnt pathway. In vivo, daily administration of PTH is sufficient to accelerate root regeneration by locally inhibiting Wnt/β-catenin signaling. Through RNA microarray analysis, lncRNA LITTIP (LGR6 intergenic transcript under intermittent PTH) is identified as a key regulator of cementogenesis under intermittent PTH. Chromatin isolation by RNA purification (ChIRP) and RNA immunoprecipitation (RIP) assays revealed that LITTIP binds to mRNA of leucine-rich repeat-containing G-protein coupled receptor 6 (LGR6) and heterogeneous nuclear ribonucleoprotein K (HnRNPK) protein. Further co-transfection experiments confirmed that LITTIP plays a structural role in the formation of the LITTIP/Lgr6/HnRNPK complex. Moreover, LITTIP is able to promote the expression of LGR6 via the RNA-binding protein HnRNPK. Collectively, our results indicate that the intermittent PTH administration accelerates root regeneration via inhibiting Wnt pathway. The lncRNA LITTIP is identified to negatively regulate cementogenesis, which activates Wnt/β-catenin signaling via high expression of LGR6 promoted by HnRNPK.
Mice ; Animals ; Cementogenesis ; Wnt Signaling Pathway ; beta Catenin/metabolism* ; Heterogeneous-Nuclear Ribonucleoprotein K/metabolism* ; RNA, Long Noncoding/genetics* ; Parathyroid Hormone ; Receptors, G-Protein-Coupled/metabolism*

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.