3.STUDIES ON HARMFUL MICROBES IN RECIRCULATING WATER SYSTEM OF HEATING PIPELINE IN TAIYUAN
Xing-Hua WANG ; Jian-Rong HAN ; Xiao-Jun HOU ; Zheng CHEN ;
Microbiology 1992;0(02):-
The microbial counts, type, as well as relationship between microbial counts and the temperature of water in reticulating water system of heating pipeline in Taiyuan were studied, which the main biofouling harmful microbes included slimeforming heterotrophic bacteria, sulfate reducing bacteria, iron bacteria and fungi, respectively. The results showed that the harmful microbes in water system were lower than that of control guideline during heating period, whereas the microbes were higher than that of control guideline, which would result in biofouling of water tube during non- heating period.
4.The effect of light exposure at night on retinal neovascularization in a mouse model of oxygen-induced retinopathy
Rong, SUN ; Chang-zheng, CHEN ; Yi-qiao, XING ; Ling, XU ; Ling-li, WANG ; Xia, ZHOU
Chinese Journal of Experimental Ophthalmology 2012;30(7):593-597
Background Oxygen-induced retinal neovascularization is the main pathological basis for many retinal vascular diseases.Research showed that light exposure at night can suppress retinal neovascularization in oxygen-induced retinopathy(OIR),but there were few reports discussing its effect on ROP.Objective This study aimed to observe the effect of light exposure at night on retinal neovascularization in an OIR mouse model.Methods Sixty-four newborn C57 BL/6J mice were randomly divided into four groups,with 16 mice for each group.OIR models were established by rearing the newborn C57BL/6J mice with their mothers in a(75±2)% oxygen environment from postnatal day 7(P7)to Pl2,and then transferred to room air.In the OIR model group,the environmental illumination level was the same as the normal control group,and the model mice were exposed to 100 lx light at night in the OIR+ light exposure group.In the simple light exposure group,normal mice were reared in room air and were exposed to light at night from P12 to P17.All the mice were sacrificed on P17,and retinal flat mounts were prepared to assess the oxygen-induced changes of retinal vessels using the adenosine diphosphatase(ADPase)histochemical technique.The amount of proliferative neovascularization was quantified by counting the number of endotheliocyte nuclei in new vessels extending from the retinal inner limiting membrane into the vitreous in ocular cross-sections.The expression of the vascular endothelial growth factor(VEGF)protein was detected by immunohistochemistry.Real-time PCR analysis was performed to examine the expression of VEGF mRNA.The rearing and usage of the animals complied with the Statement of ARVO.Results Less free-vascular areas and new blood vessels were seen in the OIR+light exposure group compared with the OIR model group.On day 17 of the mouse life,the number of the endotheliocyte nuclei in new vessels extending from retinal inner limiting membrane were 0.97±0.83,1.00±0.72,38.57±5.01 and 16.92±3.39 in the normal group,simple light exposure group,OIR model group and OIR+light exposure group,respectively,showing significant differences among them(F =78.767,P =0.000).The number of nuclei in the OIR+light exposure group were less than that of the OIR model group(t=20.446,P<0.01).Immunochemistry showed that the expression of VEGF in retina was weaker in the OIR+light exposure group than the OIR model group.The relative expression values of VEGF mRNA were 1.00±0.00,0.94±0.07,2.08±0.50 and 1.43±0.21 in the normal group,simple light exposure group,OIR model group and OIR+light exposure group,respectively,showing a significant difference (F=11.268,P =0.003),where the VEGF mRNA level in the OIR+light exposure group was lower than that of the OIR model group(t =20.163,P<0.05).Conclusions Light exposure at night can weaken retinal neovascularization in OIR mice
5.Comparison of Pattern-pulse and Pattern-reversal Multifocal Visual Evoked Potentials in Normal Individuals.
Rong-ting ZHU ; Xing-ben LIU ; Xiao-ming XU ; Chuan-fei ZHENG
Journal of Forensic Medicine 2016;32(1):35-39
OBJECTIVE:
To compare pattern-pulse multifocal visual evoked potential (PPmfVEP) with pattern-reversal multifocal visual evoked potential (PRmfVEP), and to investigate the symmetry of mfVEP between both eyes in normal individuals.
METHODS:
The multifocal Vision Monitor was used to observe the mfVEP. T-test and ANOVA were used to analyze P1 wave, amplitude and signal noise ratios (SNR) of two mfVEPs.
RESULTS:
The SNR and the P1 amplitude reached the maximum at the central visual field and decreased with the increase of eccentricity, and then decreased slowly. The amplitude of the PPmfVEP was significantly smaller than the PRmfVEP in the central retina, while in the peripheral retina the result was exactly the opposite. SNR and amplitude of the PRmfVEP showed no statistical difference in both eyes (P > 0.05). The variance of the amplitude at the same side of visual field was larger than that at the symmetrical visual quadrant.
CONCLUSION
mfVEP can reflect the visual function in different parts of retina objectively and exactly. PPmfVEP reflect the vision function of the central retina better than PRmfVEP. The stability of PPmfVEP is better than PRmfVEP in the central retina, while the result is opposite in the peripheral retina. The mfVEP is symmetrical in both eyes of the same individual.
Evoked Potentials, Visual/physiology*
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Humans
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Neurologic Examination
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Reference Values
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Retina
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Sensitivity and Specificity
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Visual Fields/physiology*
6.Microtensile bond strengths of three dentin adhesive systems.
Cui HUANG ; Xiang-Rong CHENG ; Tie-Li ZHENG ; Zhi-Xing ZHANG
Chinese Journal of Stomatology 2004;39(6):496-500
OBJECTIVETo evaluate in vitro the microtensile bond strengths of three dentin adhesive systems and their respective fracture modes.
METHODSA total of 15 intact young human premolars extracted for orthodontic reasons were used. The enamel of occlusal surfaces of these premolar teeth was removed and superficial dentine was exposed, finished with wet 600-grit silicon carbide paper. And then these teeth were randomly divided into three groups. A block of composite resin was bonded respectively with three dentin adhesive systems: All-bond 2 (Group AB(2)), Fluoro-Bond (Group FB) and Xeno III (Group Xeno) according to manufacturers' instructions. The bonded teeth were kept in distilled water for 24 h at 37 degrees C. The roots were removed from the remaining crown approximately 1 - 2 mm below the cemento-enamel junction with a slow-speed diamond saw. The teeth were sectioned to obtain bar-shaped specimens, whose bonded surface areas were about 0.8 mm(2). The specimens were stressed at a crosshead speed of 1 mm/min until rupture of the bond. SEM was used to observe the fracture modes. The mean bond strengths were compared using one-way ANOVA and LSD tests. The frequency of fracture modes was compared using Krukal-Wallis and Mann-Whitney U-test.
RESULTSMean microtensile bond strengths were (29.56 +/- 5.47) MPa for Group AB(2), (15.81 +/- 7.67) MPa for Group Xeno, and (14.61 +/- 4.50) MPa for Group FB. The bond strength of Group AB(2) was greater than those of the other two groups (P < 0.01). The bond strengths of Group Xeno and Group FB were not significantly different. SEM examination indicated that the adhesive failure was the most mode of fracture.
CONCLUSIONSThe microtensil bond strengths of three dentin adhesive systems to normal human dentine were different and the total-etching adhesive All-Bond 2 exhibited the greatest bond strength. It was recommended that dentin adhesive agent should be used according to clinical situation.
Adolescent ; Dental Bonding ; Dentin ; ultrastructure ; Dentin-Bonding Agents ; chemistry ; Humans ; In Vitro Techniques ; Materials Testing ; Microscopy, Electron, Scanning ; Tensile Strength ; Young Adult
7.Effect of P13K/AKT signal pathway regulation on expression of XIAP and cIAP2 in ovarian cancer cells.
Na TAN ; Hong ZHENG ; Jia-jia HUANG ; Xiao-rong YANG ; Xing-long WU ; Ying ZHA
Chinese Journal of Pathology 2013;42(9):613-614
Adenocarcinoma
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metabolism
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pathology
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Apoptosis
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drug effects
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Baculoviral IAP Repeat-Containing 3 Protein
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Chromones
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pharmacology
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Enzyme Inhibitors
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pharmacology
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Female
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Humans
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Inhibitor of Apoptosis Proteins
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genetics
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metabolism
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Morpholines
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pharmacology
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Ovarian Neoplasms
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metabolism
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pathology
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Phosphatidylinositol 3-Kinases
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genetics
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metabolism
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Proto-Oncogene Proteins c-akt
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genetics
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metabolism
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RNA, Messenger
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metabolism
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Signal Transduction
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Ubiquitin-Protein Ligases
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X-Linked Inhibitor of Apoptosis Protein
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genetics
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metabolism
8.HCV RNA assessment by PCR technique for screening post-transfusion HCV infection among blood donors.
Wenge XING ; Hongyi XU ; Rong MA ; Huaijing ZHENG
Chinese Journal of Hepatology 2002;10(3):211-212
OBJECTIVETo survey the application of PCR for screening HCV RNA from blood donations within the window period.
METHODSAccording to a standardized method, 12 blood banks organized by the National Center for Clinical Laboratories collected and prepared about ten thousands specimens. The specimens were tested with two different kits.
RESULTSAmong the 7173 specimens A group, 21 were PCR positive for HCV RNA. The positive rate was 0.29%. There were not positive for HCV RNA among 7477 specimens (B group).
CONCLUSIONSIt is feasible to use the PCR screening for the detection of HCV RNA of blood donations but is unnecessary to standardize the specimen collection and the kit selection.
Blood Donors ; Blood Transfusion ; Hepacivirus ; genetics ; isolation & purification ; Hepatitis C ; prevention & control ; Humans ; Mass Screening ; Polymerase Chain Reaction ; RNA, Viral ; analysis
9.Apoptosis-related gene expression and its clinical significance of human osteosarcoma.
Xing WU ; Zheng-rong CHEN ; Guang-jian ZHANG
Chinese Journal of Oncology 2004;26(11):678-681
OBJECTIVETo explore the prognostic markers in osteosarcoma.
METHODSExpressions of p53, c-myc, bcl-2 and apoptosis index (AI) in 28 osteosarcoma specimens were detected by ABC immunohistochemistry and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labelling (TUNEL). The relationship between gene expression and apoptosis, and their correlations with pathologic classification and prognostic factors were analyzed.
RESULTSThere was negative correlation between the expressions of p53, c-myc, bcl-2 protein and AI, which was closely related to the long term survival of patients but was not related to pathologic types of the tumor.
CONCLUSIONThe expressions of p53, c-myc, bcl-2 protein and AI can be used as an index for predicting the progression and prognosis of osteosarcoma.
Adolescent ; Adult ; Apoptosis ; Biomarkers, Tumor ; Bone Neoplasms ; metabolism ; pathology ; Disease Progression ; Female ; Follow-Up Studies ; Humans ; Male ; Osteosarcoma ; metabolism ; pathology ; Prognosis ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Survival Rate ; Tumor Suppressor Protein p53 ; metabolism ; bcl-2-Associated X Protein
10.Diagnostic value of contrast-enhanced fluid-attenuated inversion-recovery MRI for intracranial tumors in comparison with post-contrast T1W spin-echo MRI.
Zheng-rong ZHOU ; Tian-zhen SHEN ; Xing-rong CHEN ; Wei-jun PENG
Chinese Medical Journal 2006;119(6):467-473
BACKGROUNDContrast-enhanced fluid-attenuated inversion-recovery (FLAIR) magnetic resonance imaging (MRI) has been reported to have higher sensitivity for detecting leptomeningeal disease compared with contrast-enhanced T1-weighted MRI (CE T1WI). However, currently there are no studies showing the potential value of clinical applications of contrast-enhanced FLAIR (CE FLAIR) sequence in diagnosing intracranial tumors in a larger group of patients. The purpose of this study was to evaluate the diagnostic value of CE FLAIR in comparison with CE T1WI for intracranial tumors and to provide more information for clinical diagnosis and therapy.
METHODSOne hundred and four consecutive cases of intracranial tumors referred for CE brain MRI were analyzed with regard to FLAIR and T1WI pre- and post-administration of Gd-DTPA. The CE FLAIR and CE T1WI were evaluated independently by two radiologists for the number of examinations with one or more enhanced lesions, the number and location of enhanced lesions per examination, signal-to-noise ratio (SNR) and contrast-enhancement ratio (CER) of lesions, as well as the size and extent of the enhanced lesions.
RESULTSIn 98 of 104 cases, enhanced lesions were seen both on the FLAIR and T1W images. More lesions were seen on CE T1WI (n = 120) than those on CE FLAIR sequence (n = 117), but no differences of statistical significance were found between the two sequences (P > 0.05). Four lesions were revealed only on the CE FLAIR images whereas 7 lesions were only found on CE T1WI. Enhanced lesions located in the cerebral hemisphere or the forth ventricle were revealed much more on CE T1WI than on CE FLAIR images. However, CE FLAIR images may be useful in showing superficial abnormalities and those located in the sulcus or lateral ventricle. The CER and contrast-to-noise ratio (CNR) on CE T1WI was significantly higher (t = 7.10, P = 0.00; t = 9.67, P = 0.00, respectively), but grey matter/white matter contrast was lower (t = 2.46, P = 0.02) than those on CE FLAIR images. The SNR did not show any statistically significant difference between the two sequences (t = 1.1, P = 0.27). The size and extent of lesions on the CE FLAIR images were significantly larger than those on CE T1WI (t = 4.13, P = 0.00).
CONCLUSIONSCE FLAIR and CE T1WI may complement each other in showing intracranial tumors and the CE FLAIR sequence should be selected as a routine MRI sequence.
Adolescent ; Adult ; Aged ; Brain Neoplasms ; diagnosis ; pathology ; Child ; Child, Preschool ; Contrast Media ; Female ; Gadolinium DTPA ; Humans ; Image Enhancement ; Magnetic Resonance Imaging ; methods ; Male ; Middle Aged