1.Effects of Transcription Factor MZF-1 on Transcriptive Regulation of Acute Monocytic Leukemia-related Gene MLAA-34.
Bo LEI ; Wang-Gang ZHANG ; Ai-Li HE ; Yin-Xia CHEN ; Xing-Meim CAO ; Peng-Yu ZHANG ; Wan-Hong ZHAO ; Jian-Li WANG ; Jie LIU ; Xiao-Rong MA ; Yan-Ping ZHANG ; Hui ZHANG
Journal of Experimental Hematology 2019;27(5):1463-1468
OBJECTIVE:
To investigate the transcriptional regulation of transcription factor MZF-1 on acute monocytic leukemia-related gene MLAA-34.
METHODS:
The effect of MZF-1 on the transcriptional activity of MLAA-34 gene promoter was analyzed by luciferase reporter gene detection system and site-directed mutation technique. The EMSA and ChIP assay were used to verify whether MZF-1 directly and specifically binds to the core region of MLAA-34 promoter. The over-expression vector and interference vector of MZF-1 were constructed to transfect U937 cells, and RT-PCR and Western blot were used to detect the transcription and expression changes of MLAA-34 gene.
RESULTS:
The transcription factor MZF-1 had a regulatory effect on MLAA-34 gene expression, and the relative luciferase activity was decreased after MZF-1 binding point mutation (P<0.01). EMSA and ChIP experiments demonstrated that MZF-1 could directly bind to MLAA-34 promoter and play a regulatory role. In the over-expression test, the increase of MZF-1 could up-regulate the expression of MLAA-34 (P<0.05). In the interference test, the decrease of MZF-1 could down-regulate the expression of MLAA-34 (P<0.05).
CONCLUSION
Transcription factor MZF-1 can bind to the transcriptional regulatory region on the promoter of MLAA-34 gene and promote the transcription of MLAA-34 gene in acute monocytic leukemia.
Antigens, Neoplasm
;
genetics
;
Apoptosis Regulatory Proteins
;
genetics
;
Gene Expression Regulation, Neoplastic
;
Genes, Reporter
;
Hepatocyte Nuclear Factor 1-alpha
;
Humans
;
Kruppel-Like Transcription Factors
;
metabolism
;
Leukemia, Monocytic, Acute
;
Promoter Regions, Genetic
;
Transcription, Genetic