Adult ; Animals ; China ; epidemiology ; Female ; Hemorrhagic Fever with Renal Syndrome ; epidemiology ; virology ; Humans ; Male ; Middle Aged ; Rodentia ; virology

The traditional Chinese medicine (Tripterygium wilfordiiHook.f.,TWH) has been clinically used to treat primary and secondary renal diseases and proteinuria for nearly 40 years.However,there is a rare literature about the effect of triptolide (the main active ingredient of TWH) on the expression of oxidative carbonyl protein (OCP) in diabetic nephropathy (DN).This study aimed to provide experimental evidence for triptolide treatment on DN through its effect on the expression of OCP,in order to investigate the effects of triptolide on the expression of OCP in rats with DN.Sixty SD rats were randomly divided into five groups:control group,high-dose triptolide (Th) group,low-dose triptolide (T1) group,DN model group,and positive control (benazepril) group.The DN model was established using streptozotocin.Urinary protein excretion,fasting blood glucose (FBG),superoxide dismutase (SOD) in renal homogenate,malondialdehyde (MDA) in renal homogenate and renal nitrotyrosine by immunohistochemistry,and the expression of OCP by oxyblotimmune blotting were detected.In the DN model group,rat urinary protein excretion and renal MDA were significantly increased,while renal SOD significantly decreased and nitrotyrosine expression was obviously upregulated in the kidney.After triptolide treatment,24-h urinary protein excretion (61.96±19.00 vs.18.32±4.78 mg/day,P＜0.001),renal MDA (8.09±0.79 vs.5.45±0.68 nmol/L,P＜0.001),and nitrotyrosine expression were decreased.Furthermore,renal OCP significantly decreased,while renal SOD (82.50±19.10 vs.124.00±20.52 U/L,P＜0.001) was elevated.This study revealed that triptolide can down-regulate the expression of OCP in the renal cortex of DN rats.

To develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of epirubicin hydrochloride (EPI) in rat plasma, daunorubicin hydrochloride was used as internal standard. The plasma samples were deproteinated with methanol, and separation was performed on a reversed-phase CAPCELL PAK C18 column (3.0 mm x 50 mm, 3 microm). The mobile phase contained methanol-0.1% formic acid (80:20). Detection was carried out by multiple reaction monitoring on a HP1200-6410 QQQ LC/MS system. Different preparations of EPI solution, EPI-LIP (EPI-liposome) and EPI-LTSL (EPI-thermosensitive liposome) was administered in rats by i.v with the same dosage (12 mg kg(-1)). The pharmacokinetic model and parameters were fitted and calculated by the DAS ver2.0 software. The calibration curve was linear in the range of 0.01-50 microg mL(-1). The limit of quantification was 0.01 microg mL(-1). RSDs of intra- and interbatch precisions were all less than 11.9%. The average extract recovery was 89.3% and 92.1%, respectively. The pharmacokinetics of EPI in rats with all preparations were fitted to three compartments, which all fast distributed and slowly eliminated. The t1/2 alpha, t1/2 beta, t1/2 gamma, AUC(0-infinity), and MRT(0-infinity) of EPI-LTSL group were 7.5, 1.3, 12.6, 12.9, 3.7 times those of EPI solution group; and 1.6, 1.4, 12.3, 2.9, 2.6 times those of EPI-LIP group. Moreover, the CL of the latter two groups was about 13.4 times of the former EPI-LTSL group. EPI-LTSL can significantly improve AUC and prolong the circulation time of EPI in rat plasma.
Animals ; Antibiotics, Antineoplastic ; administration & dosage ; blood ; pharmacokinetics ; Area Under Curve ; Chromatography, Liquid ; Drug Carriers ; Epirubicin ; administration & dosage ; blood ; pharmacokinetics ; Liposomes ; blood ; pharmacokinetics ; Male ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results ; Sensitivity and Specificity ; Tandem Mass Spectrometry

OBJECTIVETo investigate the effect of Si' an Capsule combined Enalapril on hypertensive trough/peak ratio (T/P) and circadian rhythm of blood pressure in patients with essential hypertension (EH).

METHODSSixty patients with EH of stage II, III were randomly divided into two groups, the 30 patients in the treated group were treated with Si' an capsule combined Enalapril, and the 30 patients in the control group were treated with Enalapril alone. All patients were given 24-hour ambulatory blood pressure monitoring before and after 4 weeks treatment. T/P of systolic and diastolic blood pressure (SBP & DBP) of each group were calculated. Circadian rhythm of blood pressure was observed at the same time.

RESULTST/P of SBP and DBP in the treated group was 62.4 +/- 7.0% and 66.3 +/- 8.1% respectively, which was obviously higher than that in the control group, 53.3 +/- 6.7% and 60.1 +/- 7.2%, respectively (P < 0.05). The blood pressure circadian rhythm recovering rate in the treated group was 73.3% and in the control group 50%, the difference was insignificant.

CONCLUSIONThe combination therapy of Si' an capsule and Enalapril could lower the blood pressure smoothly and restore the circadian rhythm of blood pressure in EH patients.

Aged ; Antihypertensive Agents ; therapeutic use ; Blood Pressure Monitoring, Ambulatory ; Capsules ; Circadian Rhythm ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Enalapril ; therapeutic use ; Female ; Humans ; Hypertension ; drug therapy ; Male ; Middle Aged ; Phytotherapy

Antigens, Neoplasm ; Brain ; metabolism ; pathology ; Humans ; Infant, Newborn ; Lung ; metabolism ; pathology ; Male ; Melanoma-Specific Antigens ; Melanosis ; complications ; congenital ; metabolism ; pathology ; Neoplasm Proteins ; metabolism ; Neurocutaneous Syndromes ; complications ; congenital ; metabolism ; pathology ; S100 Proteins ; metabolism ; Skin ; metabolism ; pathology

Objective To study the effect of intensive trunk muscle training on balance and walking in pa- tients with hemiplegia caused by stroke.Methods A total of 90 stroke patients were recruited and randomly divid- ed into a treatment group(45 cases)and a control group(45 cases).All the patients were given conventional reha- bilitation training.Meanwhile,intensive trunk muscle training was also administered for those in the treatment group as well.The trunk control function,balance ability and walking ability were assessed by using the trunk control test, Berg Balance Scale and the balance subscale of the Fugl-Meyer physical performance test,and Holden's functional ambulation classification,respectively,before and after 6 weeks of training.Results It was found that all the pa- tients scored better with the trunk control test,Berg's balance scale,the balance subscale of the Fugl-Meyer physical performance test and Holden's ambulation classification after treatment,and there were significant differences between the two groups after treatment(P

Objective To explore the usage of Shi Jie Yin (Monosialotetrahexosylganglioside) with early comprehensive nursing intervention on the potential development of premature children.Methods 320 cases of premature children were randomly divided into intervention group and control group.The intervention group with 160 cases was given Shi Jie Yin with early rich environment intervention and comprehensive rehabilitation functional training in addition to conventional treatment.Control group only received conventional treatment,parental guidance and assessments.After discharging,children in both groups were followed up till 5 years old according to the 0 ～3 years outline of intellectual development in children and education programs.Results Scores of intervention group were significantly higher than the control group in behavioral and general condition according to NBNA [ (9.32 ± 0.33) and (5.94 ± 0.83 ) for intervention group,(6.35 ± 1.35 ) and (4.39 ± 0.42) for the control group; t =9.573,3.590,respectively; P ＜ 0.0 1 ].In the month with completed neurological and psychological developments,children in control group showed significantly less raising head at the supine position,upright of head,turn over,conscious pronouncing and laughing,grabbing toys with fingers,sitting steady with free hands and crawling than those of the intervention group (t =3.29,4.27,3.62,2.98,4.62,4.52,4.51,respectively ;P ＜0.01 ).The CDCC scale monitoring of the two groups included development index (MDI) and physical development index (PDI).Intervention group had higher scores in both indexes than the control group[ (95.46 ± 12.35 ) and ( 98.87 ± 12.20) vs ( 79.12 ± 1 4.71 ) and ( 84.03 ± 1 3.37 ) ; t =5.761,6.832,respectively;P ＜ 0.01 ].Comparing two groups of the development of intelligent energy region and the total developmental quotient,the intervention group was significantly higher than the control group ( P ＜0.01 ).For retardation and the incidence of complications,the intervention group was significantly lower than the control group ( P ＜ 0.05 ).Conclusions Application of Shi Jie Yin with early comprehensive nursing intervention in the potential development of premature children,together with comprehensive system of rehabilitation functional training,adequate treatment and follow-up treatment could reduce the incidence of cerebral palsy and mental retardation and complications,promote the overall development potential of premature children,and improve the quality of survival of premature children and population quality.

AIM To establish the HPLC fingerprints of Qingfeiyucuo Pills (an agent for the management of acne,containing Scutellariae Radix,Eriobotryae Folium,Paeoniae Radix Rubra,etc.) and to determine the contents of four constituents.METHODS The analysis of methanol extract of Qingfeiyucuo Pills was carried out on a 35 ℃ thermostatic Agilent HC-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.4％ phosphoric acid flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Eleven common peaks with the similarities of more than 0.9 were observed in the HPLC fingerprints of ten batches of samples.Paeoniflorin,hesperidin,baicalin and salvianolic acid B showed good linear relationships within the ranges of 0.08-0.25 μg,0.07-0.22 μg,0.2-0.60 μg and 0.06-0.18 μg,whose average recoveries were 98.7％,96.9％,97.4％ and 98.2％ with the RSDs of 0.98％,1.09％,0.82％ and 1.66％,respectively.CONCLUSION This sensitive and specific method can be used for the quality control of Qingfeiyucuo Pills.

Objective To evaluate therapeutic effects of oseltamivir treatment in patients with mild novel influenza A ( H1N1) virus infection through a retrospective analysis.Methods Clinical data of 308 patients with influenza A (H1N1) was collected from 3 infectious diseases hospitals during May-August 2009,after exclusion of non-eligible cases,289 patients were divided into control group and oseltamivir treatment group,the duration of fever and viral load were compared between the 2 groups.Results Influenza A (H1N1) mostly affected 10 to 29-year-old persons,fever was the most common symptom which was low or moderate-level( ＜ 39 ℃ ),other symptoms involved sore throat,cough,headache,and rhinorrhea,a few patients had vomiting (1.3％)and diarrhea( 3.9％ ).Finally,there was no significant difference in duration of virus shedding from illness onset or beginning of therapy among the 2 groups(P =0.32,0.93 ).Conclusion The adolescents were main target of influenza A (H1N1) virus,most patients appeared to experience mild disease with better prognosis.For mild patients,oseltamivir could not shorten duration of fever and virus shedding effectively,and the virus could be cleared rapidly by immune system.Oseltamivir treatment had no clinical significance for mild influenza patients.

Baculoviridae ; genetics ; metabolism ; Phylogeny ; Viral Proteins ; chemistry ; classification ; genetics ; metabolism