1.Hedgehog signal regulates the chondrogenesis from bone marrow mesenchymal stem cells:controlling methods and cross-talking relationship with other signals need further studies
Chinese Journal of Tissue Engineering Research 2014;(37):6040-6045
BACKGROUND:The hedgehog pathway has paid an important role in the progress of chondrogenesis from bone marrow mesenchymal stem cells. However, the definite signal transduction pathway and cross-talking relationship with other common signal pathways are stil poorly understood and the researches related to this field is to continue as a hotspot in the future study. OBJECTIVE:To investigate the research progress of hedgehog signal pathway on the regulation of the chondrogenesis from bone marrow mesenchymal stem cells and the relationship between hedgehog and other signal pathways in the process. METHODS:A computer-based online search in CNKI, PubMed and Google Scholar databases was performed using key words of“Hedgehog, IHH, SHH, bone marrow mesenchymal stem cells, cartilage, chondrogenesis”in English and Chinese, respectively. Literatures related to the process of chondrogenesis from bone marrow mesenchymal stem cells were included and 36 articles were extensively summarized for review. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells are currently accepted optimal cellseeds for the cartilage tissue engineering, and hedgehog is a critical signal molecule in the development of skeletal system. The IHH and SHH in hedgehog signal closely participate in control ing the processes of bone marrow mesenchymal stem cellproliferation and chondrogenesis, chondrocyte phenotype maintenance and cooperation with other common single pathways. However, the specific signal transduction mechanism and cross-talking contact with other signal pathways stil need to be further studied, and it stands for the future research directions.
2.Study on the Legal and Ethical Issues in the Disposal of Frozen Embryos
Lidan AO ; Xing XING ; Changcui WU ; Xin LIU
Chinese Medical Ethics 2017;30(2):202-205,210
Along with the application and development of the assisted reproductive technology in vitro fertilization and embryo transfer,the emergence of frozen embryos in the law,medicine,and ethics area are gradually increased.Two-child policy makes more older couples choose assisted reproductive technology,which will cause more disputes about frozen embryos.There is no unified conclusion of the legal status of human embryos,and there exists three main views of embryos namely property,person and intermediate state.Defined the legal status of frozen embryos as ethical substance,this paper analyzed the main position and possible legal and ethical issues involved in the disposal of frozen embryos through three typical cases.
3.Study on the Anti-inflammatory,Antalgic Effects and Irritation of Fuyan Suppository
Wenbo WU ; Lichen SUN ; Xing LIU
China Pharmacy 2015;(25):3504-3506
OBJECTIVE:To study the anti-inflammatory,antalgic effects and irritation of Fuyan suppository. METHODS:Mouse auricle swelling test(Fuyan suppository 4.5,3.0 and 1.5 g/kg for administration)and mouse vola pedis swelling test(5 min and 0.5,1,4,6 h)were employed to study the anti-inflammatory effect of Fuyan suppository. Mouse writhing response test(Fuy-an suppository 4.5,3.0 and 1.5 g/kg) was used to study the antalgic effect. Rabbit vagina mucosa irritation test was adopted to study the vaginal irritation(Fuyan suppository 1.0 g/rabbit,once daily for one week). RESULTS:Fuyan suppository at a dose of 4.5,3.0 or 1.5 g/kg could inhibit auricle swelling of mice and reduce the times of mice’s writhing response. 3.0 g/kg Fuyan suppos-itory could,at 0.5-6 h from administration,inhibit vola pedis swelling of mice and be nonirritating to the rabbit’s vagina. CON-CLUSIONS:Fuyan suppository has better anti-inflammatory and antalgic effects and is nonirritating to the rabbit’s vagina. Theoreti-cally,vaginal administration of Fuyan suppository can be considered as safe.
4.Curriculum reform of urogenital system integration and reflections on the curriculum integration
Xiangmei WU ; Huarong YU ; Liu TU ; Juan QIAO ; Xing LIU
Chinese Journal of Medical Education Research 2021;20(1):22-25
Current reform of higher medical education focuses on curriculum integration and corresponding reconstruction of teaching system. Chongqing Medical University has carried out the reform of medical personnel training mode from basic to clinical, which has achieved certain results. Three aspects including teaching content, teaching methods and appraisal system of the urogenital system curriculum integration are introduced in the paper. In the teaching content, the parts related to urogenital system in basic subjects and clinical subjects are extracted and integrated. In the process of teaching implementation, a teaching team is set up across departments and multidisciplinary joint teaching is carried out. In order to arouse the enthusiasm of students, inspire and cultivate students' scientific research thinking by improving teaching methods, several teaching methods such as case introduction teaching method, question discussion and debate meeting are used. Scientific evaluation system is used to observe and record the whole process of students' learning. Meanwhile, some existing problems and solutions of curriculum integration are discussed in the paper to provide references for peers in relevant colleges and universities.
5.Cloning, expression,purification and immunological identification of Der f 11, paramyosin,a recombinant allergen of dust mite
Congli JIANG ; Yulan WU ; Peng XING ; Pingchang YANG ; Zhigang LIU
Chinese Journal of Immunology 2014;(6):736-739
Objective: To clone , express and purify of Dermatophagoides farinae ( Der f 11 ) , and then test its immunogenicity.Methods:The gene coding for Der f 11 was synthesized ,and was then linked with the pET-32a vector.The expression plasmid pET32a(+)-Der f 11 was induced by IPTG.After purification of recombinant allergens Der 11 proteins through the Ni +affinity chromatography ,immunological allergic patients serum as the Primary antibody.Results: We obtain high purity recombinant Der f 11 protein.The results of SDS-PAGE show that the expression product is about 118 KD.Recombinant allergen Der f 11 test 15 dust mites allergic patients serum specific IgE , positive rate was 20%.Conclusion: Recombinant allergen Der f 11 obtained has the similar immunologic activity to natural Der f 11 protein.It can lay the foundation for the specific diagnosis ,treatment and further experimental studies of the dust mite allergy disease.
6.Effects of Qinggan Huoxue Recipe and its separated recipes on the expression of tumor necrosis factor-alpha in rats with alcoholic liver injury.
Tao WU ; Tao LIU ; Peiyong ZHENG ; Lianjun XING ; Guang JI
Journal of Integrative Medicine 2008;6(11):1145-51
To study the effects of Qinggan Huoxue Recipe (QGHXR), the compound traditional Chinese herbal medicine, and its separated recipes on the expression of tumor necrosis factor-alpha (TNF-alpha) mRNA and serum TNF-alpha content in rats with alcoholic liver injury (ALI).
7.The use of new teaching method in practical teaching of the digestive system medical imaging
Wu XING ; Xiaoyi WANG ; Jinkang LIU ; Lihong OU
Chinese Journal of Medical Education Research 2011;10(9):1105-1106
The authors use the method of combination of PBL teaching and clinic practicing in practical teaching of the digestive system medical imaging.The process is divided in four steps.This method can greatly improve the learning efficiency of the students and get good teaching result.
8.Role of angiotensin Ⅱ in the regulation of platelet-derived growth factor receptor β subunit of vascular smooth muscle
Dongqi XING ; Hua BAI ; Yinping SUN ; Jie LIU ; Liling WU
Chinese Journal of Pathophysiology 2001;17(6):485-488
AIM: To investigate the crosstalk between angiotensin Ⅱ (AngⅡ)-mediated and platelet-derived growth factor (PDGF)-mediated signal transduction in vascular smooth muscle proliferation.METHODS: A model of renal hypertension was made by two kidney/one-clip operation. Level of PDGF receptor β subunit of aorta was measured by Western Blot analysis. The effect of Ang Ⅱ on PDGF receptor β subunit expression was investigated in culture rat aortic vascular smooth muscle cells (VSMC).RESULTS: Systolic blood pressure obviously increased at 8th week after operation, whereas the level of PDGF receptor β subunit of aorta significantly increased by 126.6% (P<0.05) in 2K1C rats compared with control group. The expression of PDGF receptor β subunit in cultured VSMC stimulated by AngⅡ was higher than that of control by 192.74%(P<0.01). The effect of AngⅡ was inhibited remarkably by pretreated with losartan, a kind of specific AngⅡ receptor 1 (AT1) subtype antagonist and U73122, a kind of phospholipase C inhibitor. The effect was partly blocked by PD98059, which inhibit the activity of mitogen-activated, ERK-activating kinase (MEK).CONCLUSION: AngⅡ-induced PDGF receptor β subunit expression is regulated by the AT1 and its downstream signal molecule-PLC and ERK, might participate in the intracellular signal transduction pathway.
9.SFE-CGC DETERMINATION OF TANSHINONE IN SALVIA MILTIORRHIZA BUNGE
Bin CHEN ; Mei ZHU ; Wongxing XING ; Lili LIU ; Yutian WU
Acta Pharmaceutica Sinica 2001;36(1):55-57
AIM To analyze tanshinone IIA in Salvia miltiorrhiza Bunge by supercritical fluid extraction (SFE) coupled with capillary gas chromatography (CGC). METHODS The solution pressure was predicted by solubility parameter theory, the extraction conditions were confirmed by orthogonal design in which temperature, volume of modifier and dynamic extracting volume had been studied in three levels. RESULTS This method is quick, convenient, precise and accurate, added sample recovery is 95.3%, RSD=4.27%. CONCLUSION Compared with the method of ultrasonic extraction, significantly better qualitative and quantitative result could be obtained by SFE-CGC and expend less organic solvent then make less pollution.
10.Experimental study of the effects of olfactory lamina propria transplantation and ganglioside GMI treatment on spinal curd injury
Zhonghao LIU ; Mingzhi GONG ; Shiqing WU ; Deguo XING ; Ruoyi WANG
Chinese Journal of Geriatrics 2009;28(5):418-420
Objective To observe the effects of olfactory lamina propria (OLP) transplantation and ganglioside GM1 treatment on spinal cord injury (SCI) in rats.Methods Totally 50 healthy pure breed female Sprague-Dawley (SD) rats after spinal cord hemiseetion were randomly divided into 5 groups and were given different treatments: (OLP + GM1) treatment group (group A), GM1 treatment group (group B), OLP treatment group (group C), spinal cord injury but without treatment group (group D) and healthy control group (group E). The recovery of neurological function was evaluated by somatosensory evoked potential (SEP) and pathological examination after surgery. Results In group A, in some rats an escaping response in right hind leg occurred, but in other groups, the motor function was not significantly improved. Histological examination showed that transplanted olfactory lamina propria survived in the transplantation area and expanded on certain routes. NF positive nerve fibers passed through the transplantation area. Compared with group B, C, D, the N1-wave latency was(4.71±0. 72)ms 4 weeks after operation(P<0. 01), and the NF density was(7. 31±0. 26) ×104/mm28 weeks after operation in group A(P<0. 05). Conclusions Olfactory lamina propria (OLP) transplantation and ganglioside GM1 treatment have a synergistic effect on SCI.