3.Experimental study on NK cells promoting donor marrow engraftment and hematopoietic reconstitution after MHC haploidentical BMT in mice.
Chun-yan WANG ; Kun-yuan GUO ; Bing-yi WU ; Lan-xiao WU ; Zhen-yu JIANG ; Xing-hua PAN
Chinese Journal of Hematology 2004;25(5):290-292
OBJECTIVETo explore the effect of donor-derived NK cells added to pretreatment conditioning regimen on hematopoietic reconstitution after MHC haplotype-mismatched BMT in mice.
METHODSMurine model of MHC haplotype-mismatched BMT was established by using BALB/c(H-2d) x C57BL/6(H-2b) (CB6F(1)(H-2d/b)) mouse as recipient, and C57BL/6(H-2b) mouse as donor. Fifty recipient mice were divided into 5 groups. The mice in the first three groups were each infused 1 x 10(6), 5 x 10(5), 2 x 10(5)/mouse donor-derived NK cells, respectively before TBI ((60)Co, 9.0 Gy) and then conditioned with TBI, followed by infusion of C57BL/6(H-2b) mice bone marrow cells four hours later. The mice in the fourth group received TBI only, and in the fifth group, TBI and BMT at the some doses as the first three groups. Hematopoietic reconstitution, survival time, body weight, histopathology of the recipients were followed up.
RESULTS(1) Survival time was (5.15 +/- 0.66) days for the fourth group, and > 30 days for the other 4 groups. (2) Leukocyte and platelet counts at day 10 after BMT were (0.99 +/- 0.22) x 10(9)/L and (61.0 +/- 7.27) x 10(9)/L respectively for the fifth group and (2.01 +/- 0.21) x 10(9)/L, (101.50 +/- 16.34) x 10(9)/L; (1.98 +/- 0.29) x 10(9)/L, (99.50 +/- 16.41) x 10(9)/L and (1.97 +/- 0.21) x 10(9)/L, (98.0 +/- 16.19) x 10(9)/L for the first three groups, respectively. Histopathology displayed no GVHD in all the groups.
CONCLUSIONDonor-derived NK cells could promote hematopoietic reconstitution after MHC haplotype-mismatched BMT in mice.
Animals ; Bone Marrow Transplantation ; Female ; Graft Survival ; Graft vs Host Disease ; immunology ; prevention & control ; Haplotypes ; Histocompatibility Testing ; Killer Cells, Natural ; cytology ; immunology ; Lymphocyte Transfusion ; Major Histocompatibility Complex ; immunology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Transplantation Conditioning ; methods ; Transplantation, Homologous
4.Study on the metabolite of stilbene glucoside in mice by liquid chromatography tandem-mass spectrometry.
Jiang-hao SUN ; Lan-tong ZHANG ; Chun-ying WANG ; Zhi-fang YUAN ; Xing-hong LU
Acta Pharmaceutica Sinica 2003;38(12):968-970
AIMTo study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry.
METHODSThe stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C18 column (250 mm x 4.6 mm ID, 5 microns). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15:18:66:1) for ES+, acetonitrile-methanol-water (15:18:67) for ES-. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L.h-1. The ion source temperature was 150 degrees C.
RESULTSThe stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity.
CONCLUSIONIt was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.
Animals ; Chromatography, Liquid ; methods ; Female ; Glucosides ; blood ; isolation & purification ; metabolism ; Male ; Mice ; Plants, Medicinal ; chemistry ; Polygonum ; chemistry ; Spectrometry, Mass, Electrospray Ionization ; methods ; Stilbenes ; blood ; chemistry ; isolation & purification ; metabolism
5.The regulatory effects of arginine on hepatic insulin-like growth factor-1 secretion in rats and its implication.
Xiu-ling MA ; Chang-Jiang GUO ; Zhan-hui GENG ; Ji-jun YANG ; Jing-yu WEI ; Lan-xing GAO
Chinese Journal of Burns 2005;21(4):255-258
OBJECTIVETo explore the regulatory effect of arginine on the secretion of hepatic insulin-like growth factor-1 (IGF-I), and the mechanism of enhancing the immune function by arginine.
METHODSWistar rats were randomly divided into normal control (NC), wound control (WC), and wound with arginine (Arg) groups, with 8 rats in each group. The rats in WC and Arg groups were inflicted with soft tissue trauma on the back. The rats in Arg group were fed a diet supplemented with 5% arginine for one week, while those in NC and WC groups were fed with glycine. The serum contents of arginine, ornithine, growth factor (GH), NO and IGF-I were determined 7 days after feeding. T cell proliferation and IGF-I mRNA expression in hepatic tissue were also measured. Meanwhile, the rat hepatocytes were cultured in serum-free medium containing different concentrations of arginine. The supernatant was collected for the determination of IGF-I level.
RESULTS1). There was no obvious difference of the serum level of arginine and ornithine between NC and WC groups (P > 0.05), but the contents of them were obviously higher in the Arg group compared with other two groups (P < 0.01). 2). No difference in the serum GH level was found among all the groups (P > 0.05), but the serum NO content in WC and Arg groups was significantly lower than that in NC group (P < 0.01), and the serum IGF-I content in WC group decreased obviously compared with that in NC group (P < 0.01). 3). The thymocyte proliferation rate in WC group was also markedly lower than that in NC group (P < 0.01), but that in Arg group was improved compared with WC group (P < 0.01). 4). The expression of hepatic IGF-I mRNA: The relative value of IGF-I mRNA was 1.19 +/- 0.06, 1.08 +/- 0.06 and 1.29 +/- 0.06 in NC, WC and Arg, respectively, while the value in WC was lower than that in NC (P < 0.05) group, and that in Arg group was much higher than that in WC group (P < 0.01). 5). The IGF-I level in the supernatant of cultured hepatocytes: When Arg concentration was 0.0750, 0.7500, 7.5000 mmol/L in the culture medium, the IGF-I level in the supernatant of hepatic cell medi-um was obviously higher than that in the medium without arginine (P < 0.01). Although IGF-I level decreased in the culture medium with arginine in the dose of 37.5000 mmol/L, it was still obviously higher than that in the medium without arginine (P < 0.01).
CONCLUSIONArginine could also produce the immune enhancing effect by stimulating hepatic IGF-I secretion.
Animals ; Arginine ; pharmacology ; Enteral Nutrition ; Insulin-Like Growth Factor I ; metabolism ; Liver ; drug effects ; secretion ; Male ; Rats ; Rats, Wistar ; Soft Tissue Injuries ; metabolism ; therapy
6. Analysis of the cardiovascular effects on workers expose to occupational benzene-toluene-xylene and noise in a painting workshop of an automobile company
Hailin ZHOU ; Lan JIANG ; Shiqi MAI ; Jinwei ZHANG ; Zhi WANG ; Xing RONG ; Yimin LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2019;37(4):280-283
Objective:
To learn about the cardiovascular health effects of workers expose to benzene-toluene-xylene and noise in painting workshop of automobile manufacturing enterprises, and to provide intervention measures and strategies for the health of workers.
Methods:
The effects of noise exposure, benzene-toluene-xylene exposure and combined exposure on workers' blood pressure and electrocardiogram were analyzed through epidemiological investigation, workplace monitoring and occupational health examination in several automobile enterprises which had carried out occupational hazard factors monitoring and occupational health examination in our hospital from April to October 2017.
Results:
There were differences in age, sex, working years, smoking, drinking and physical exercise among workers in different exposure groups (
7.Quality of life and nursing service need in eldly low vision patients
Hui-Lan ZHENG ; Xiu-Lan LU ; Xiao-Yan ZHENG ; Mo-Jun CHENG ; Wen-Jing JIANG ; Guo-Xing XU
International Eye Science 2018;18(1):137-139
AIM:To investigate the quality of life of elderly patients with low vision and its nursing service demand,in order to improve the quality of life of elderly low vision group and provide theory basis.METHODS:A cross-sectional study method,in a tertiary hospital in Fuzhou city elderly patients with low vision 200 cases were as the research object,USES the Chinese version of quality of life scale (CLVQOL),low vision care service demand questionnaire investigation were taken.RESULTS:In elderly patients with low vision life quality score was 54.92±12.11,the nursing service demand score 30.34± 8.93,medium and high demand was of 88%.Quality of life was negatively correlated with nursing service demand value (r=-0.266,P<0.01).CONCLUSION:The quality of life of elderly patients with low vision was low,nursing service demand is high,especially on visual function recovery training of nursing service requirements.Aging brings important social problems in China,attaches great importance to the elderly care service demand,discussion with precise visual nursing care intervention in low vision group,it is necessary to improve the quality of life.
8.Effect of community intervention on recovery of patients with lumbar degenerative disease
Fei WANG ; Li SUN ; Jia-Cheng QIAN ; Xing-Lan JIANG ; Jia-Yan GE ; Jie-Sha YANG ; Ruo-Lan FU
Chinese Journal of Modern Nursing 2013;48(34):4217-4220
Objective To discuss the effect of community intervention on visual analogue scale ( VAS ) , Oswestry disability index ( ODI ) and frequency of drug use in patients with lumbar degenerative disease ( LDD) .Methods Forty-one patients with LDD were applied with 3 months of community intervention , self-made DV on health guide and handbook on rehabilitation training were handed out , community lecture was carried out and telephone interview was conducted .VAS and ODI were used to evaluate patients ’ pain and function of lumbar vertebra , and the frequency of drug use was collected 3 months before and after the intervention.Results The correct rate of training on lumbar posture and dorsal muscles was 92.68% after the intervention, higher than 17.07% before the intervention, and the difference was statistically significant (χ2 =47.33,P<0.001).The score of VAS on lumbar and VAS on lower limbs , the frequency of drug use and the score of ODI was respectively (2.65 ±0.82),(2.60 ±1.01),(1.51 ±0.71),(10.95 ±4.52) before intervention, and (2.05 ±0.69),(1.94 ±0.87),(0.98 ±0.57),(8.46 ±3.49)after the intervention, with statistically significant differences (t=6.82,7.07,6.81,9.87, respectively; P <0.00).Conclusions Community intervention can promote the improvement of symptoms and recovery of body function for patients with LDD .
9.Effects of amiloride on potassium and calcium currents in guinea pig ventricular myocytes.
Lan CHENG ; Fang WANG ; Hong-Yi ZHOU ; Wei-Xing YAO ; Guo-Jin XIA ; Ming-Xing JIANG
Acta Pharmaceutica Sinica 2004;39(7):509-513
AIMTo elucidate the possible mechanisms underlying antiarrhythmia of the non-selective Na+/H+ exchanger inhibitor--amiloride.
METHODSSingle ventricular cells were isolated using a double-enzyme method. Effects of amiloride on voltage-dependent potassium and calcium currents in isolated guinea pig ventricular myocyte were recorded by using whole-cell patch clamp techniques.
RESULTSExposure to amiloride (10 -100 micromol x L(-1)), the L-type and T-type calcium currents were depressed. Amiloride resulted in a concentration-dependent inhibition of peak (Ca,L), But amiloride did not change the shape of their I - V curves. It only decreased the amplitudes of the currents of the two types. When myocytes were incubated with 100 micromol x L(-1) amiloride, I(Kr) was slightly depressed and I(Ks) did not change. Amiloride (1 - 100 micromol x L(-10) depressed I(K1) in a concentration-dependent manner.
CONCLUSIONAmiloride depressed potassium and calcium currents, which may give support to its uses in some diseases of the cardiovascular system.
Amiloride ; pharmacology ; Animals ; Anti-Arrhythmia Agents ; pharmacology ; Calcium Channels, L-Type ; drug effects ; Calcium Channels, T-Type ; drug effects ; Cell Separation ; Female ; Guinea Pigs ; Heart Ventricles ; cytology ; Male ; Myocytes, Cardiac ; drug effects ; Patch-Clamp Techniques ; Potassium Channels, Inwardly Rectifying ; drug effects ; Potassium Channels, Voltage-Gated ; drug effects ; Sodium-Hydrogen Exchangers ; antagonists & inhibitors
10.A prospective cohort study on the risk of HBV infection in preschool children without immune response to hepatitis B vaccine
Hui-sen HUANG ; Na WEI ; Mei-dan LAN ; Zuo-jie CEN ; Xing-xing MENG ; Jin-tai JIANG ; Lu YANG ; Zhi-chun XIE
Chinese Journal of Disease Control & Prevention 2019;23(1):15-18
Objective To evaluate the risk of hepatitis B virus(HBV) infection among preschool children who were the non-responders to hepatitis B vaccine in future. Methods A prospective cohort study was conducted. Children aged 2 to 5 years were selected from 64 kindergartens.These children were inoculated three doses of hepatitis b vaccine at 0, 1 and 6 months after birth. Hepatitis B surface antigen (HBsAg)and Hepatitis B surface antibody (anti-HBs)were detected during the period from March to May 2015. The children who were HBsAg negative were enrolled in the study. The subjects were divided into exposure group (anti-HBs negative) and control group (anti-HBs positive) . The follow-up began on June 1, 2015 and ended on June 1, 2016. Serum HBsAg of children in the cohort was then collected and detected from June 1 to 30, 2016. At the end of the study, the HBsAg positive rates between two groups were compared. Results 83 children who received hepatitis B vaccine again during the follow-up period were excluded from 1 907 non-responders. The actual number in non-responders group was 1 824. 151 children were lost at the end of the study. The actual number of follow-up was 1 673 and 5 children were found to be positive for HBsAg and the infection rate was 0.30% (5/1673). In the respondent goup, 2 054 were enrolled and followed. Finally, 140 children were lost and none of the remaining 1 914 people were HBsAg positive at the end of the study. HBsAg positive rate was higher in the non-responder group than in the responder group (P=0.023). Conclusion There is a risk of HBV infection in the children who are non-responders to hepatitis B vaccine in future.