1.The changes of calmodulin and cAMP in rabbit myocardium during ischemia and reperfusion
Chinese Journal of Pathophysiology 1986;0(01):-
This study was performed on rabbits to examine the changes of calmodulin (CAM) and cAMP in the myocardium during ischemia and reperfusion by radioimmunoassay method. The result showed the levels of CaM and cAMP increased at 20 min of ischemia. Although these two parmeters were unchanged at 40 min of ischemia as compared with the changes at 20 min, they were changed during reperfusion at different times of ischemia. At 20 min ischemia following 10 min reperfusion, the content of CaM decreased, the content of cAMP increased. On the basis of 40 min ischemia, reperfusion induced opposite changes: CaM increased, cAMP decreased. The possible mechanism of these changes was discussed.
2.Obstructive sleep-disordered breathing in infants.
Xiao-hong CAI ; Liang-xing WANG
Chinese Journal of Pediatrics 2013;51(10):753-755
Continuous Positive Airway Pressure
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Facial Bones
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abnormalities
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Humans
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Infant
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Larynx
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pathology
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Oxygen Inhalation Therapy
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Pharynx
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pathology
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Polysomnography
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Respiration
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Risk Factors
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Sleep Apnea Syndromes
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diagnosis
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etiology
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therapy
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Sleep Apnea, Obstructive
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diagnosis
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etiology
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therapy
4.Effect of selective cycloxygenase-2 inhibitor NS-398 on IL-1α and TNF-α expression in HaCaT cells induced by ultraviolet rays
Yinghua SONG ; Hong LIANG ; Xing DAI ; Xiaoming LIU ; Shahshan WANG
Chinese Journal of Medical Aesthetics and Cosmetology 2012;18(1):52-55
Objective To study the effect of selective COX-2 inhibitor NS-398 on IL-1α and TNF a expression in HaCaT cells induced by UVA/UVB,and further to explore the mechanism on anti human skin photo damage. Methods The subcuhured HaCaT cells were divided into three groups:simple illuminated group was exposed to UVA/UVB directly,NS-398 interfered group was exposed to UVA/UVB after being treated with NS-398 in different concentrations,and the control group was cultured in normal without any treatment.The expression of IL -1α and TNF-α in supernarant was detected by ELISA kit.Results The level of IL-1α and TNF-α expression in supernatant from simple illuminated group was remarkably higher than that in the control group,NS-398 interfered group showed much lower level than the simple illuminated one,and the expression of IL-1α and TNF-α was dependent on the concentration of NS-398.Conclusions NS-398 can reduce IL-1α and TNF-α expression in HaCaT cells induced by ultraviolet rays,suggesting the possibility of anti human skin photo- damage effect.
5.Effect of selective cycloxygenase-2 inhibitor NS-398 on IL-10 and IFN-γ mRNA expression in hunman keratinocytes induced by long-wave ultraviolet
Shanshan WANG ; Hong LIANG ; Xing DAI ; Xiaoming LIU ; Yinhua SHONG
Chinese Journal of Medical Aesthetics and Cosmetology 2012;18(3):204-207
Objective To study the effect of selective COX-2 inhibitor NS398 on IL-10 and IFN-γexpression in human keratinocytes induced by UVA,and further to explore the effect on anti human skin photoaging.Methods HaCaT cells were divided into three groups:simple UV exposure group,NS398-intervene group and blank group.For UV radiation,simple UV exposure group and NS398-intervene group were irradiated with UVA at a dose of 30 J/cm2.In order to assess the effect of NS398,HaCat cells were treated with NS398 at the dose of 0,20,40,80μmol/L respectively and then incubated without irradiation for 2h,substrate changed and cultured for 24h,and then the expression of IL-10 and IFN-γ mRNA was detected via RT-PCR.Results Simple UV exposure group had obviously higher expression of IL-10 mRNA and lower expression of IFN-γ mRNA than that of control group,while NS398-intervened group had significantly lower expression of IL-10 mRNA and higher expression of IFN-γ mRNA than that of simple UV exposure group,and dose-dependency existed.Conclusions NS398 may delay photo-damage of human skin via inhibiting the expression of IL-10 mRNA and up-regulating the expression of IFN-γ mRNA in human keractinocytes.
6.Pesticide Degrading Microorganisms
Fu-Xing ZHU ; Mo WANG ; Jian-Hong LI ;
Microbiology 1992;0(05):-
This paper was mainly on pesticide degrading microorganisms. The major pesticide degrading bacteria were Pseudomonas, Bacillus, Flavobacterium, Alcaligenes, Arthrobacter, etc. The major pesticide degrading fungi were Aspergillus,Pinicielium, Rhizopus, Trichoderma, Fusarium, etc. The major pesticide degrading actinomycetes were Nocardia, Streptomyces, etc.
7.High-level Secretion Expression of Human ScFv Against Botulinum Neurotoxin A in Pichia pastoris*
Hui WANG ; Jun YIN ; Xiao-Jun HOU ; Hong-Guang XING ;
Microbiology 1992;0(02):-
The specific ScFv gene against botulinum neurotoxin A (BoNTa)was cloned into pPIC9k. Positive integrators were screened by increasing the dose of G418 in culture and expressed in Pichia pastoris GS115. As a result, engineered recombinant clone were obtained. 26 kD product of interest was seen easily in SDS-PAGE. Expression of human ScFv got the highest level 15% of total secreted proteins during 72~84 h after 1% methanol inducing. Purification of ScFv was finished by two steps: gel filter and ion exchange. Competing ELISA showed that recombinant ScFv could compete with antiserum to specific bind BoNTa.
8.Surgical treatment of complex humeral nonunion
Ping ZHEN ; Xing-Yan LIU ; Hong-Dong WANG ;
Chinese Journal of Orthopaedic Trauma 2004;0(09):-
Objective To discuss the effects and methods of the surgical treatment of complex humeral nonunion.Methods Ten cases of scleroid humeral nonunion were treated by compressive interlocking medullary nailing and autografting of iliac bone.Fifteen cases of humeral nonunion combined with osteomyelitis or osteoporosis were treated with grafting of free vascularized fibular flaps.Results All the cases were followed up for six months to six years.They all obtained bone union.The average time of bone union was 3.1 months for the free vascularized fibular grafting group and 3.8 months for the interlocking medullary nail group.Conclusions Compressive interlocking medullary nailing and autografting of iliac bone is effective for scleroid humeral nonunion,while grafting of free vascu- larized fibular flaps is effective for humeral nonunion combined with osteomyelitis or osteoporosis because the free vas- cularized fibular grafting can well reconstruct the defective humerus bone with osteoporosis or osteomyelitis.
9.Studies on twelve cytokines in serum of coronary heart disease patients
Xing-Wang JIA ; Ya-Ping TIAN ; Guang-Hong GUO ;
Chinese Journal of Laboratory Medicine 2003;0(09):-
Objective The significance of serum cytokine profile in coronary heart disease patients have been evaluated by observing the variation cytokines Methods The serum IL-1 a,IL-1 ?,IL-2,IL-4, IL-6 ,IL-8,IL-10,VEGF,IFN-?,,TNF-?,MCP-1 and EGF in 76 patients and 26 matched healthy volunteers have been observed.Serum cytokines had been measured by protein chips methods in EVIDENCE 180 automatic biochips analyzer and cytokine profile were got by bioinformatics.Results The serum level of IL-1?,IL-6,IL-8,and TNF-? were (4.32?8.14) ng/L ,(13.16?16.81) rig/L,(375.53?292.14) ng/L,(15.46?15.38) ng/L respectively,which increased obviously in patients (P
10.The effect of window setting technique on measuring colon disease in CT virtual colonoscopy
Xing-Wang WU ; Bin LIU ; Hong ZHAO ; Yong-Qiang YU ;
Chinese Journal of Radiology 2001;0(03):-
Objective To study the value of lung window and soft tissue window in measuring colon diseases with experimental CTVC examination.Methods Twenty-four artificial polypoid diseases were placed with glue in a thoroughly cleaned porcine colon(overall length,150 era)that was distented with air and submerged in a water phantom and scanned 10 times on a 64-MSCT(GE Light speed)with identical scanning parameters.The data were reconstructed every 0.625 mm and reviewed using 2D images, navigation,MPR and 3D volume-rendered images on a GE AW 4.2 workstation.The maximum diameters of the artificial polypoids were measured by two radiologists with more than 10 years working experience in lung window(W = 1000,L =-700)and soft tissue window(W =400,L =40),respectively.The average value of maximum diameters of the artificial polypoids were measured for 10 times and were compared with the actual diameter.Results Twenty-four artificial polypoid diseases and their shape,size,loeation and relation of diseases with bowel wall were all showed excellently.The maximum diameter measured was close to the actual diameter.The average value was mostly less than actual diameter and a few of them more than the actual diameter.The maximum diameter measured in lung window was close to the actual diameter,and there was no difference between the two data sets(t =0.431,P =0.669).There was difference between the maximum diameter measured in soft tissue window and the virtual diameter(t = 2.691,P = 0.010). Conclusion In screening the colon diseases,CTVC is a good method of choice with no insult or less insult, and higher repeatability.The lung window should be chosen in measuring colon diseases in CTVC examination.