Aim A UVA-induced apoptotic model of HaCaT cells was established to investigate the impact of UVA on c-jun/cyclooxygenase-2(COX-2)and explore related molecular mechanism of the polypeptide from Chlamys farreri(PCF)protecting HaCaT cells from UVA-induced apoptosis.Methods Cells were divided into five groups:control group,UVA model group,UVA+5.69 mmol?L-1 PCF group,UVA+2.84 mmol?L-1 PCF group,UVA+1.42 mmol?L-1 PCF group.Expression level of c-jun was assayed by Real-Time PCR and Western blot.RT-PCR and Western blot analysis were used to determine the mRNA and protein levels of COX-2.Using agarose gel electrophoresis,the effects of PCF and COX-2 inhibitor celecoxib on UVA-induced apoptosis were also investigated.Results PCF and celecoxib had inhibitory effect on 8 J?cm-2 UVA-induced apoptosis of HaCaT cells.COX-2 mRNA and protein levels increased after UVA radiation and the discrepancy was significant compared with control group(P

Humans ; Neck ; Solitary Fibrous Tumors

Objective To evaluate the appl ication of CT perfusion in intra-axia l disease. Methods By applying a nuclear medicine data processi ng technique to the time-density data obtained from dynamic CT, cerebral tissue perfusion was p e rformed in 8 cases, 4 with normal findings, 2 with ischemic cerebrovascular dise ase, and 2 with gliomas. Cerebral blood perfusion maps was created and analyzed, absolute regional cerebral blood volume, regional cerebral blood flow, and mean transit time were determined and analyzed as well. Results Wit h regard to var ious degrees of perfusion impairment within the ischemic territory, thus giving rise to a very heterogenous appearance. As a rule, the center of a lesion usuall y representing the core of ischemia showed marked reduction of blood flow. At th e outer margin of a tumor area, the reduction of CBF and CBV was often less pron ounced, indicating some residual flow via collaterals, as was confirmed by the b olus delay on the time to peak time. Conclusion In the near fut ure, functional imaging techniques should have a major impact on therapeutic decision-making i n acute stroke patients, perfusion CT may be recommended even now for emergency applications in daily patient care due to its practicability.

? Choroidal neovascularization is the primary pathogenesis of neovascularage - related macular degeneration ( nAMD ) , and the role of vascular endothelial growth factor ( VEGF ) in neovascularization has been widely recognized. Currently, drugs target different targets of VEGF have been widely used in the treatment of nAMD. As a subtype of VEGF, placental growth factor ( PlGF) has synergistic effects with VEGF-A on promoting angiogenesis, stimulating the migration of endothelial cell proliferation and mediating immune inflammatory response. There is no expression of PlGF in mature blood vessels so PlGF hashigh specificity. ln this paper, the role of PlGF in the pathogenesis and treatment of nAMD is reviewed.

Objective To investigate the relationship between metabolic syndrome (MS) and recurrence of urolithiasis.Methods A retrospective analysis was performed in urinary stone patients from March 2008 to February 2012.Patients were divided into MS group and non-MS group according to the diagnose criteria of metabolic syndrome (2007 version) by the joint committee for developing Chinese guidelines on prevention and treatment of dyslipidemia in adults.The patients were followed up for 24-72months (median 47 months) since operation.The difference of stone recurrence was compared between the 2 groups.Results Two hundred and eighteen patients with urinary stone disease were enrolled.Of them,52 patients were diagnosed with MS.Stone recurrence occurred in 29 patients (55.8％) of MS group,whereas 66 patients (39.8％) of non-MS group suffered stone recurrence.It demonstrated the median recurrence free survival of group MS and non-MS was 36 months and 59 months by Kaplan-Meier analysis,respectively (Log-rank test,P =0.019).Multivariate Cox regression analysis results revealed that MS was significantly associated with stone recurrence (HR 1.817,95％ CI 1.105-2.988,P =0.011),however,the gender (P =0.336),age (P =0.246) and recurrence urolithiasis at first visit (P =0.051) were not associated with stone recurrence.Conclusions MS is an independent risk factor for urinary stone recurrence.It is suggested that the treatment of MS may have a important role in prevention of stone recurrence in MS patients with urolithiasis.

Objective To study the reliability and validity of Chinese version of housebound scale in Chinese elderly people in community.Methods The housebound scale was amended and translated according to the Procedure of the European organization for Research and Treatment of Cancer (EORTC) Quality of Life Group.Include two scales,which is whether is housebound and the reason of housebound.The reliability and validity of Chinese version of housebound scale were tested in a sample of 860 Chinese elderly people in two communities among 127 communities in Tangshan from October 2012 to March 2013.Reliability was evaluated using the splithalf reliability and internal consistency.Validity was assessed by content validity,discriminate validity and construct validity.Results The Cronbach’s α coefficient of the first subscales was 0.743,the split-half reliability was 0.856,the convergent validity ratio was 0.960,and the scale had good discriminate validity (P ＜ 0.01).The Cronbach's αcoefficient of the second subscales was 0.853,the split-half reliability was 0.816,the convergent validity ratio was 0.910.Four factors body and mental factors,environmental factors,psychological and social factors,listening factors were extracted by a factor analysis,which explained 48.37％ of the total variance.The loading value of each items in their respective common factor ranged from 0.42 to 0.82.Conclusion The housebound scale has better reliability and validity,which is available for the evaluation of housebound amnong elderly people.

Objective To investigate the efficacy and safety of combination of transcatheter arterial chemoembolization (TACE)and percutaneous radiofrequency ablation (RFA)in treatment of hepatocellular carcinoma (PHC).Methods 70 cases of PHC were divided into combined group (TACE +RFA,n =37) and control group (only TACE,n =33).Patients were followed up for1 to 2 years,and the therapeutic effects and side effects were compared between the two groups.Results The patients with tumor completely necrosis and AFP level lower than >50% in combined group were significantly more than those in control group (P <0.05);the half of year,one year and two years survival rate in combined group were greatly higher than those of control group;no severe side effect was observed in two groups.Conclusions TACE+RFA is effective and safe in treatment of PHC of more than 5 cm in diameter.

Objective To summarize surgical results of secondary tunnel-like subaortic stenosis( STSS) after congenital heart disease( CHD) operations, analyze the pathogenesis of STSS, evaluate the operative effect and prognosis.Methods We analyzed clinical data of 25 patients who underwent surgical repair for STSS in Fuwai Hospital from January 2009 to December 2015.There were 17 males and 8 females.CHD types included double outlet right ventricle, ventricular septal defect and par-tial atrioventricular septal defect, et al.The median age of the patients when they received their first CHD operations was 1 year (1 month to 42 years).The median age of the patients when they received TSS repair was 5 years and 8 months(2 years and 10 months to 48 years) .The surgical types contained modified Konno procedure , Konno procedure, resection of the conal sep-tum, resection of fibromuscular ridge and myectomy, removal and reconstruction of the intraventricular baffle.Results All the patients successfully received their TSS repair .There was no surgical death in this study .The preoperative gradient of aortic valve pressure was 81(43 to 159)mmHg(1 mmHg=0.133 kPa), and the postoperative gradient was 8.2(4.0 to 46.2) mm-Hg.Ⅲ degree atrioventricular block was occurred in 2 patients after operation and both 2 patients needed to set permanent pacemaker.All the patients were followed up after discharge for a median duration of 2 years( 4 months to 6 years) .During follow-up, none of the patients had any clinical symptom or subaortic restenosis , and there was no late death .Conclusion The pathogenesis of STSS is perhaps related to turbulent flow and shear stress to the left ventricular outflow tract , resulting in the proliferation of fibromuscular marterials and stenosis in the left ventricular outflow tract .The modified Konno procedure and resection of the conal septum are safe and effective.

OBJECTIVE:To study the curative effects of Shenyanqing tablet.METHODS:1 200 patients with chronic glomerulonephritis,acute glomerulonephritis,latent glomerulonephritis and purpura glomerulonephritis were randomized into the treatment group and control group,and separately studied on curative effects and reduction of adverse reaction of glucocorticosteroid.In the study of curative effects,the control group received no special treatment except that on reducing pressure and diuresis,and the treatment group was given Shenyanqing tablet,8 tablets? d-1,tid,in addition to receiving same treatment as that for the control group.In the study of reducing adverse reaction of glucocorticosteroid,the control group(the treatment group was the same as the method above)was given normal glucocorticosteroid treatment for 3 months and 2 months respectively.RESULTS:It has significant difference(P