Animals ; Disease Models, Animal ; Female ; Fibrosis ; metabolism ; pathology ; Hydroxyproline ; metabolism ; Lung ; drug effects ; metabolism ; pathology ; Mice ; Thrombospondin 1 ; pharmacology

Objective To observe activated cerebral areas during the task of writing in second language by early and late acquisition bilinguals and explore the neuropsychological mechanism. Methods Twelve subjects were divided into group of early acquisition high proficiency bilinguals(EAHP) and group of late acquisition high proficiency bilinguals(LAHP). Each subject carried out the task of pseudo-writing and English writing. After functional imaging,statistical parametric mapping was used to compare data of the two tasks through paired- t test in both two groups. A conjunction analysis was conducted between the two groups also. Areas where the metabolism of glucose changed were analyzed. Then, the corresponding images about activated cerebral regions were obtained.Results Cerebral areas where the metabolism of glucose increased in EAHP: bilateral middle frontal gyrus( Z=4.35/2. 01 ), bilateral cerebellum ( Z = 2.97/2.53 ) and left head of caudate nucleus ( Z = 3.07 ), etc. Cerebral areas where the metabolism of glucose increased in LAHP: right superior frontal gyrus(Z=4.06), right cerebellum( Z=4.63), left putamen(Z=2.70) ,etc. Cerebral areas where the metabolism of glucose increased in compared data of LAHP with data of EAHP: right frontal gyrus ( Z = 2.92 ), right temporal lobe( Z = 2.31 ), etc. Conclusion Extensive cerebral cortex and subcortical structures participate the process of writing in the second language. Age of bilingual acquisition is one of the factors affecting cerebral localization of writing in second language.

Objective To study the sonographic features of papillary thyroid carcinoma (PTC) associated with cervical lymph nodes metastasis for early diagnosis and prediction of the invaded cervical lymph nodes. Methods The sonographic features of 170 patients with pathologically confirmed PTC in First Afifliated Hospital of Harbin Medical University between 2011 and 2013 were retrospectively reviewed. There were 59 cases with neck lymph nodes metastases and 111 cases without neck lymph nodes metastases. Receiver operating characteristic (ROC) curve was aaplied to analyze the cut-off values of resistance index (RI) and peak systolic velocity (PSV) for judging the presence or absence of cercical lymph node metastasis. The Chi-square test and rank sum test were used to compare the different sonographic features between each group. The Logistic regression analysis was used to obtain the relevant factors of PTCs with cervical lymph node metastasis. Results ROC curve analysis showed that the cut-off values of RI and PSV were 0.735,13.95 cm/s. The primary tumor diameter, the existence of halo, the involvement of thyroid upper pole, the microcalciifcation, the blood suply classiifcation and the RI, PSV were statistically signiifcantly different between PTCs with and without cervical lymph node metastasis, whereas no statistical signiifcance was detected between the primary tumor echo pattern, boundary and the longitudinal/transveral ratio between the metastatic and nonmetastatic group. Logistic regression analysis showed that the PTC primary tumor diameter and PSV were independent factors coorelated with cervical lymph node metastasis. Conclusion Some sonographic features of PTC are closely correlated with lymph nodes metastasis, which are valuable in predicting the cervical lymph nodes metastasis in patients with PTC pre-operatively.

Objective To analyze the influencing factors of quality of life of patients with occupational pneumoconiosis in Methods Chongqing using a structural equation model. A total of 1 181 pneumoconiosis patients in Chongqing were selected - as the research subjects using convenient sampling method. The Chinese version of the SF 36 Scale was used to investigate the quality of life of the patients. Amos 24.0 software was used to construct a structural equation model reflecting the quality of life of Results ， （ ），（ ） （ ）， patients. The scores of physical health mental health and quality of life were 61.7±18.5 68.7±20.5 and 64.7±16.8 respectively. The scores of the eight dimensions of the quality of life of the research subjects were lower than that of the （ P ） Chongqing City municipal and national norms all <0.01 . Multiple linear regression results showed that personal monthly ， ， ， income pneumoconiosis stage complications employment status and smoking were the influencing factors of the quality of life （ P ） ， of pneumoconiosis patients all <0.05 . The results of the structural equation model showed that the stage of pneumoconiosis ， ， complications personal monthly income and pulmonary rehabilitation treatment could directly affect the physical and mental （ P ） ， health of the research subjects all <0.05 . In addition the stage of pneumoconiosis and complications indirectly affected the （ P ）Conclusion physical and mental health of patients by affecting pulmonary rehabilitation treatment all <0.05 . The structural equation model can be used to verify the influencing factors of the quality of life of patients with occupational pneumoconiosis. ， Complications personal monthly income and pneumoconiosis stage are the main factors affecting the quality of life of patients with occupational pneumoconiosis.

Objective To observe clinical effects of treating subarachnoid hemorrhage with intracranial aneurysm interventional embolization combined with Chinese herbs of removing blood stasis and hemostasis. Methods 45 cases with subarachnoid hemorrhage were randomly reeurited into a control group and a treatment group. The control group was treated with intracranial aneurysm interventional embolization, and the treatment group was treated with intracranial aneurysm interventional embolization combined with Chinese herbs with the functions of removing blood stasis and hemostasis. The clinical effect was observed between the groups. Results The clinical effect in the treatment group was significantly higher than that of the control group (P< 0.05 ). Conclusion The treatment of subarachnoid hemorrhage with intracranial aneurysm interventional embolization combined with Chinese herbs of removing blood stasis and hemostasis is better than intracranial aneurysm interventional embolization exclusively.

AIM:To investigate the roles of ClC-3 chloride channels in the regulation of cell cycle and the re-lationship between ClC-3 chloride channels and the cell cycle regulators , such as cyclin D1, cyclin-dependent kinase (CDK)4, CDK6, P21 and P27 in the HeLa cells.METHODS:ClC-3 genes were silenced by the siRNA technique in the HeLa cells.The transfection efficiency of ClC-3 siRNA was detected by real-time PCR.The cell cycle distribution was ana-lyzed by the flow cytometry .The protein expression of ClC-3, P21, P27, CDK4, CDK6 and cyclin D1 was determined by Western blot .RESULTS:ClC-3 was knocked down by ClC-3 siRNA in the HeLa cells .Transfection of the cells with ClC-3 siRNA arrested the cells at G0/G1 phases, decreased the expression of cyclin D1, CDK4 and CDK6, and increased the expression of P21 and P27.CONCLUSION:ClC-3 plays an important role in the cell cycle of HeLa cells through the G 1-S transition point.ClC-3 may regulate the cell cycle progression by up-regulation of cyclin D1, CDK4 and CDK6 expression and/or by down-regulation of P21 and P27 expression.

Objective To establish a method of fingerprint analysis on Huai Chrysanthemum mori-folium in Henan Province by GC.Methods The GC method was applied with a fiber column(30 m ? 320 ?m,0.25 ?m)coated with HP-5(5%)-diphenypolysiloxane.The detector was FID detector;The temperature of the detector was 180 ℃;Nitrogen was used as the carrier gas with its flow rate 2.3 mL/min;The flow rate of atmosphere was 300 mL/min,and the flow rate of hydrogen was 30 mL/min in temperature programmer.The fingerprint of Huai C.morifolium in Henan Province was established.Results Twenty-seven common peaks were found in GC fingerprint of Huai C.morifolium in Henan Province and good similarities with correlation coefficients over 0.9 were found in the fingerprints compared between the fingerprint of Huai C.morifolium in Henan Province from different sources and standard fingerprint.Conclusion The method is accuate,simple,and can be used for the quality control of Huai C.morifoliumin Henan Province.

Objective To detect the levels of cytokine IL-2,IL-12,IFN-?and IL-4 secreted by peripheral CD8~+ T lymphocytes in patients with condyloma acuminatum (CA).Methods Flow cytometry was employed to study the expression of cytokines IL-2,-12,INF-?and IL-4 in CD8~+ T lymphocytes in the peripheral blood of 60 patients with CA and 20 healthy controls.Results The percentage of CD8~+ T lym- phocytes producing IL-2,IL-12 and IFN-?were significantly lower in CA patients than that in healthy con- trols (P

Objective: To investigate the absorption and transepithelial transport characteristics of scutellarin and scutellarein in the human colonic adenocarcinoma cell (Caco-2) monolayer model. The influence factors on these two compounds' absorption were investigated, such as buffer solution, duration of culture, and inhibitors of multidrug resistance-associated protein 2 (MRP(2)), breast cancer drug resistance protein (BCRP) and P-glycoprotein (P-gp). Methods: By using Caco-2 monolayer as an intestinal epithelial cell model, the transport process was studied from apical (AP) side to basolateral (BL) side or from BL to AP. The two compounds were determined by high-performance liquid chromatography coupled with diode-array-detector detection. Transport parameters and apparent permeability coeffients (P(app)) were calculated. Results: The P(app) values of scutellarin and scutellarein were different in two buffer solutions, respectively. In phosphate buffered saline, scutellarin had no absorption from AP to BL, while its P(app) value was 0.74×10(-6) to 1.58×10(-6) cm/s from BL to AP. The P(app) values of scutellarein were 4.33×10(-6) to 6.79×10(-6) cm/s and 1.32×10(-6) to 2.56×10(-6) cm/s from AP to BL and from BL to AP, respectively. The P(app) value gradually decreased with time. The absorption of scutellarein was better than that of scutellarin. The scutellarin absorption was improved by verapamil, MK-571 and reserpine. The scutellarein absorption was improved by verapamil whereas its excretion was improved by MK-571. Conclusion: Absorption of scutellarin is difficult in Caco-2 monolayer cells, which contributes to its low bioavailability. Scutellarein absorption is better than scutellarin absorption. Scutellarein transepithelial transport is passive diffusion. The inhibitor of P-gp can improve scutellarin and scutellarein transportation. The inhibitors of MRP(2) and BCRP can promote transportation of scutellarin. The inhibitor of MRP(2) can promote efflux of scutellarein. The multidrug resistance-associated protein may be the second reason for low bioavailability of scutellarin.

Objective To observe and analyze the MRI manifestations of Klippel-Trenaunay syndrome(KTS).Methods Thirty-one cases with diagnosed KTS underwent MRI on a 1.5 T MR system. MRI,MR venography(MRV),MR angiography(MRA)and X-ray venography(XRV)were performed.The pathological changes of the limbs and their veins were observed.Results MRI found soft tissue hemangiomas in 12 cases,soft tissue swelling of the extremities in 27 cases,superficial varicosities in 21 cases,and malformation of the veins in 27 cases.In twenty patients who underwent both MRV and XRV, superficial varicosities in 17 cases and persistent sciatic veins in 11 cases were found with both techniques. The increase of tributary veins was found in 10 cases with XRV,while found in 15 cases with MRV.The erratic venous course was found in 4 cases with MRV.The abnormalities of deep veins were found in 8 cases with MRV,while found in 7 cases with XRV.Conclusion MRI is an efficient and reliable imaging method for diagnosis of KTS.