1.Hypoxia-induced caveolin-1 up-regulation is involved in migration and in-vasion of lung adenocarcinoma A549 cells
Bei ZUO ; Min XING ; Zhengui SUN ; Xianghai WANG ; Xingwu CHEN
Chinese Journal of Pathophysiology 2014;(10):1794-1799
AIM:To investigate the regulatory role of hypoxia mimic reagent cobalt chloride ( CoCl2 ) on cave-olin-1 (Cav-1) generation and the influence of Cav-1 on the abilities of migration and invasion of human lung adenocarcino-ma A549 cells.METHODS:The concentrations of Cav-1 and hypoxia-inducible factor ( HIF)-1αin pleural effusion of the patients with lung cancer ( MPE) or tuberculous pleurisy ( TBPE) were detected, and the correlation was also compared. A549 cells were treated with CoCl2 at different concentrations and time in the presence or absence of HIF-1αinhibitor YC-1.The concentrations of Cav-1 and HIF-1αin the cell supernatants were measured by ELISA.The effects of Cav-1 induced by CoCl2 on the migration and invasion of A549 cells were determined by scratch test and Transwell invasion trial, respec-tively.RESULTS:The levels of Cav-1 and HIF-1αin MPE were significantly higher than those in TBPE.There was a highly positive correlation between Cav-1 and HIF-1αlevels in the pleural effusion.CoCl2 induced the generation of Cav-1 and HIF-1αin A549 cells in a concentration-and time-dependent manner, the peak occurred at 200 μmol/L or 24 h, while the concentration over 200 μmol/L or after treated over 24 h, a concentration-or time-dependent inhibition was ob-served.HIF-1αinhibitor YC-1 concentration-dependently inhibited the generation of HIF-1αand Cav-1 induced by CoCl2 in A549 cells.CoCl2 enhanced A549 cells migration and invasion, with 200 μmol/L played the strongest role, which were down-regulated significantly in the presence of YC-1.CONCLUSION:The alteration of hypoxia-induced Cav-1 generation might be involved in the migration and invasion of A549 cells.A possible role for HIF-1αis indicated in Cav-1 generation.
2.The significance of photopic negative response in early diagnosis of primary optic nerve atrophy.
Changzheng CHEN ; Chengguo ZUO ; Yiqiao XING ; Llianfang YI
Ophthalmology in China 1993;0(04):-
0.05). But the amplitudes of the PhNR were significantly reduced in group of primary optic nerve atrophy (P
3.Expression of CXCL12-CXCR4 and its association with angiogenesis in pancreatic cancer.
Zuo-xing NIU ; Li-ming FEI ; Chang-liang WANG
Chinese Journal of Oncology 2009;31(4):286-287
Adenocarcinoma, Papillary
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blood supply
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metabolism
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pathology
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Adult
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Aged
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Carcinoma, Pancreatic Ductal
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blood supply
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metabolism
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pathology
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Chemokine CXCL12
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metabolism
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Lymph Nodes
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metabolism
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Lymphatic Metastasis
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Male
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Microvessels
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pathology
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Middle Aged
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Neoplasm Staging
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Neovascularization, Pathologic
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metabolism
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pathology
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Pancreas
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metabolism
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Pancreatic Neoplasms
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blood supply
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metabolism
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pathology
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Receptors, CXCR4
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metabolism
4.Effects of Different Dosage of Insulin-Like Growth Factor-1 on Apoptotic Protein of Myocard in Mice with Viral Myocarditis
zuo-jun, WANG ; jian, GU ; xing-yuan, HUANG
Journal of Applied Clinical Pediatrics 2006;0(18):-
Objective To explore the effects of different period of insulin-like growth factor-1(IGF-1)on B cell lymphoma/leukeamia-2 gene(BCL-2)and BCL-2 associated X protein(BAX)of myocard in mice with viral myocarditis.Methods Male Balb/c mice were randomly divided into control group,coxsackie viral B3(CVB3)infection group(infection group),IGF-1-treated group in earlier stage(0-6 d)and acute stage(7-13 d),injected IGF-1 of exogenous in abdominal cavity [30 ?g/(kg?d)].Animals were killed at the 14 day.The immunohistochemical studies and medical imagine analysis system were performed to evaluate the expression of BCL-2 and BAX in myocardial tissue.Results In infection group,the expression of BCL-2 reduced and BAX increased than those of control and treatment group(Pa
5.Rifampicin resistance among elderly patients with pulmonary tuberculosisin Changzhou City
WU Yujiao ; ZUO Xiaoming ; QIN Keyu ; ZHANG Zhixin ; ZHANG Xing
Journal of Preventive Medicine 2024;36(2):162-164,168
Objective:
To investigate the rifampicin resistance status and its influencing factors among elderly patients with pulmonary tuberculosis in Changzhou City, Jiangsu Province, so as to provide the basis for improving drug-resistant pulmonary tuberculosis prevention and control strategies.
Methods:
Patients aged over 60 years with pulmonary tuberculosis treated in tuberculosis designated hospital was selected. Demographic information, history of previous disease, history of diagnosis and treatment and drug sensitivity test were collected. Rifampicin resistance among elderly patients with pulmonary tuberculosis and its influencing factors was analyzed.
Results:
Totally 249 elderly patients with pulmonary tuberculosis were included, with an average of (69.75±4.36) years. There were 147 males and 102 females, with a gender ratio of 1.44∶1. There were 183 treatment-naïve patients (73.49%) and 66 retreated patients (26.51%). Rifampicin resistance was found in 21 cases, with a drug resistance rate of 8.43%. Among them, there were 7 treatment-naïve patients (3.83%), and 14 retreated patients (21.21%). Multivariable logistic regression analysis showed that retreated elderly patients with pulmonary tuberculosis had a higher risk of rifampicin resistance (OR=10.551, 95%CI: 1.344-82.857).
Conclusion
The rifampicin resistance rate of 249 elderly patients with pulmonary tuberculosis was 8.43% and was associated with the type of treatment.
6.CT and MRCP findings of autoimmune pancreatitis and small pancreatic carcinoma
Jiule DING ; Wei XING ; Changjing ZUO ; Shengnan YU ; Jianguo QIU ; Jing SHENG
Chinese Journal of Pancreatology 2012;12(2):79-82
Objective To increase the awareness of autoimmune pancreatitis (AIP) and correct diagnosis rate of AIP by investigating radiologic characteristic of AIP and small pancreatic cancers.Methods The clinical data and radiologic imaging of 24 AIP patients who met the Asia diagnostic criteria of AIP in 2008 and 25 cases of pathologically condirmed small pancreatic cancer were retrospectively reviewed.All imaging findings were reviewed,especially on the shape,enhancement patterns of mass,pancreatic ducts,peripancreatic and extra-pancreatic manifestations. Chi-square test and Fisher test were used for statistical analysis.ResultsThe imaging signs,including location of masses,distal pancreatic atrophy,enhancing mass during portal phase,truncated duct sign of pancreatic duct,capsule-like rim and renal involvement,was significantly different between AIP and small pancreatic cancer ( x2 =9.010,10.506,15.488,8.688,6.292 and 4.966 respectively,P <0.05 ).But only two signs,distal pancreatic atrophy and enhancing mass during arterial and portal phase,were statistically different between local AIP and small pancreatic cancer (P <0.05).ConclusionsThe typical imaging features of diffuse AIP is distinct and helpful for the differential diagnosis of AIP from small pancreatic carcinoma,but there is limited value in the differential diagnosis between local AIP and small pancreatic cancer.
7.Research on building a systematic education system in general practice
Xing HUANG ; Bo WEI ; Haiying ZHANG ; Yanli ZUO ; Ying SHEN ; Hong LI
Journal of Medical Postgraduates 2014;(6):633-635
Academic Health Systemis an effective mode to make a response to the change of medical pattern , and more and more aging population , and continued increasing of health care costs .By introducing the system to the field of general practice , using the systematic theory in three basic functions of high schools , combining medical schools with all levels of health-related institutions , building a systematic and continuity education system in general practice .it can resolve the problems such as lack of motivation , poor cooperation of the administrative department and education department , and lack of teachers and practice bases of general practice .
8.Simultaneous determination of four constituents in Xiaojie'an Capsules by HPLC
Gang BAO ; Yamin ZUO ; Jianjun HUANG ; Tian ZHOU ; Wei PAN ; Qing WU ; Xing LI
Chinese Traditional Patent Medicine 2017;39(3):536-539
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Xiaojie'an Capsules (Forsythiae Fructus,Leonuri Herba,Spatholobi Caulis,etc.).METHODS The analysis of chloroform extract of this drug was carried out on a 30 ℃ thermostatic Diamond C1scolumn(250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile (A)-0.05 mol/L monosodium phosphate (B) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 278 nm.RESULTS Berberine hydrochloride,palmatine chloride,phillyrin and rutin showed good linear relationships within the ranges of 0.033 7-0.337 2 μg (r =0.999 1),0.054 8-0.548 3 μg (r =0.999 0),0.025 9-0.258 8 μg (r=0.999 2) and 0.008 4-0.084 2 μg (r =0.999 6),whose average recoveries were 98.8% (RSD =1.3%),99.8% (RSD =0.7%),98.8% (RSD =1.3%) and 96.8% (RSD =1.0%),respectively.CONCLUSION This sensitive and accurate method can be used for the quality control of Xiaojie'an Capsules.
9.Construction and improvement of medical education system in general medicine
Xing HUANG ; Bo WEI ; Haiying ZHANG ; Yanli ZUO ; Xiaoyan PAN ; Ying SHEN ; Hong LI
Chinese Journal of Medical Education Research 2013;(12):1189-1191
Guangxi Medical University had taken a lot of measures to construct systematic general medicine education system consisting of medical college education, post graduation education and continuing medical education. These measures included constructing the organization, modifying the personnel training program, training rural-order directional medical students, standardizing resi-dent training, providing on-the-job training and postgraduate degree education for general practitioners.
10.Development of spatiotemporal frequency turning in rat lateral geniculate neuron
Xu-hong, ZUO ; Xue-feng, SHI ; Fang, XIE ; Li-min, XU ; Kan-xing, ZHAO
Chinese Journal of Experimental Ophthalmology 2012;30(5):388-391
BackgroundRecent researches suggested that properties of neurons in the lateral geniculate neuron (LGN) may represent an important neural limitation on the development of basic spatial and temporal vision,and even binocular rivalry.However,previous studies on the properties of spatiotemporal frequency tuning of LGN were rather concentrated on a monkey or cat,whereas little is known about rat.ObjectiveThis study was to examine the development of spatiotemporal frequency tuning in rats LGN.MethodsTwenty Wistar rats were collected and divided into 14-16 day,20-22 day,27-30 day and 60 day groups according to the different ages after their eyes opened and 5 rats were assigned for each group.Extracellular single neuron recording was carried out in the rats to study the spatio-temporal receptive field properties of neurons in LGN by sinusoidal gratings visual stimuli.Dynamic changes of the spatio-temporal receptive field properties of neurons in LGN with the development of Wistar rats were evaluated.ResultsThe differences between band-pass and low-pass distribution of temporal frequency or spatial frequency of rat LGN were not statistically significant (x2 =0.68,0.47,P>0.05 ).The optimal temporal frequency of receptive field in rat LGN went up to the maximum value until 60 day in Wistar rats.The mean optimal temporal frequencies of neurons in the four different age groups were ( 2.5 ± 1.3 ),( 2.6± 1.2),(2.6± 1.1 ) and ( 3.6± 1.1 ) Hz with significant differences among the 4 groups (F=4.53,P<0.05 ),and those in the 14-16 day group,20-22 day group,27- 30 day group were significantly lower than in the 60 days group ( q =4.43,4.10,4.03,P < 0.05 ).No significant differences were seen among the 14-16 day group,20-22 day group and 27-30 day group ( P>0.05 ).The optimal spatial frequency values in the four groups were ( 0.04 ± 0.04 ),( 0.04 ± 0.03 ),( 0.05 ± 0.03 ),( 0.05 ± 0.04 ) cpd,respectively without statistical difference among them ( F =0.58,P > 0.05 ).The temporal and spatial bandwidth values in the various age groups were not statistically significant among the four groups ( F =0.37,1.22,P>0.05). Conclusions The development of temporal and spatial frequency characteristics of the rat LGN receptive field may be related to its functional visual pathway.