Plasma concentrations of cyclic GMP and cyclic AMP were measured by radioimmunoassay in 114 patients with chronic atrial fibrillation, 15 patients with paroxysmal atrial fibrillation and 10 patients with paroxysmal supraventricular tachycardia. The results showed that the plasma cyclic GMP concent rations in all these three groups were significantly higher than those in the control group (21. 45 ?. 01, 23. 10?1. 66 and 20. 91?0. 92nmol/L vs 6. 23?0. 23nmol/L, respectively, P

Objective:To investigate whether plasma lipoprotein-associated phospholipase A_(2)(Lp-PLA_(2)) activity correlates with severity and stability of coronary atherosclerosis and other established cardiovascular risk factors.Methods: Coronary angiography(CAG) was performed in 180 hospitalized patients who were suspected as having coronary heart disease(CHD).The severity of pathological changes of the coronary artery was assessed by the number of diseased coronary branches and Gensini′s score.According to the results of CAG,the 180 patients were divided into 2 groups: CHD group(n=112) and non-CHD group(n=68).The CHD patients were further divided into subgroups according to the clinical types,the number of diseased coronary branches and Gensini′s score.Lp-PLA_(2) activity,white blood cell(WBC) count,high sensitive C reactive protein(hsCRP),lipids,blood pressure and body mass index were measured.The age,sex and prior medical histories including hypertension,diabetes mellitus and smoking status were obtained before CAG in all patients.Lp-PLA_(2),WBC count and hsCRP were compared statistically between the subgroups,and correlation coefficients of Lp-PLA_(2) activity with WBC count,hsCRP and other conventional risk factors for CHD were calculated.Results: Plasma Lp-PLA_(2) activity in CHD patients was significantly higher than that in controls(P

Objective: To study the related factors of artery occlusion induced cardiac infarction. Methods: The coronary angiography data of 56 patients with total artery occlusion were retrospectively studied. The patients were divided into 2 groups: Group A with cardiac infarction, Group B without cardiac infarction, and the clinical features of the 2 groups were compared. Results: It was found that diabetes mellitus, anginal pectoris, hypercholesterolemia, high and low density lipoprotein, smoking, long term medication, collateral circulation, and changes of electrocardiogram presentation were all related factors of infarction( P 6 months), long term medication, well controlled blood glucose and lipoprotein, and non smoking, probably because these factors can improve the collateral circulation and induce ischemia preconditioning.

Objective:To analyze the clinical characteristics and laboratory examinations of hospitalized children with influenza, and improve medical personal awareness of severe influenza.Methods:Data of inpatients with influenza from November 2018 to February 2019 at Shenzhen Children′s Hospital was collected, and the clinical characteristics and laboratory examinations were statistical analyzed.Results:Of the 702 cases, 464(66.1%) cases were mild influenza, and 238 cases(33.9%)were severe influenza; the median age was 3.8 years, and 457(65.1%) cases were 0 to 5 years old.The number of patients with basic diseases and wheezing symptoms in the severe group was more than those in the mild group, with statistically significant differences(all P<0.05). The white blood cell count, percentage of neutrophils, neutrophils to lymphocytes ratio(NLR), C-reactive protein and procalcitonin in the severe group were higher than those in the mild group, while the percentage of lymphocytes was lower than that in the mild group(all P<0.05). Logistic multivariate regression analysis showed that underlying diseases, wheezing and NLR were risk factors for severe influenza.The area under the curve of NLR for severe influenza was 0.574, whose best cutoff value was 1.43. Conclusion:Influenza virus infection among children is mainly influenza A, and mostly affected children are under 5 years old.The blood routine of children with severe influenza mostly showes increased white blood cell, mainly neutrophils increased significantly, and increased C-reactive protein and procalcitonin.Children with underlying diseases, wheezing symptoms during the course of the disease, and elevated NLR are more likely to progress to severe cases, and NLR cannot be used as a independent predictor of severe influenza.

Cone-beam computed tomography(CBCT) was used to analyze the anatomic morphology of maxillary canine fossa of different sagittal skeletal patterns patients;implants of various dimensions were virtually placed into the maxillary premolars region.The relationships among maxillary canine fossa and implant were analyzed.The depth of the maxillary canine fossa was(4.33±0.73),(3.77±0.58),(5.18±0.93)mm in the 1st premolar region of class Ⅰ,Ⅱ and Ⅲ respectively.The depth of the maxillary canine fossa was(3.20±0.63),(2.81±0.58),(3.90±0.79)mm in the 2nd premolar region of class Ⅰ,Ⅱ and Ⅲ respectively.There were no significant statistical differences in genders,sides,and loss of tooth.And there were significant statistical differences in three classes(P<0.05).The length of the implants was (10.30±1.70)mm(d=3.3 mm) and (8.77±1.58)mm(d=4.1 mm) in the 1st premolar region and (8.09±1.51)mm(d=3.3 mm),(6.69±1.35)mm(d=4.1 mm) in the 2nd premolar region in patients with teeth and buccal perforation;the length of the implants was (8.98±1.54)mm(d=3.3 mm) and (7.67±1.52)mm(d=4.1 mm) in the 1st premolar region and(7.09±1.59)mm(d=3.3 mm) and (5.79±1.34)mm(d=4.1 mm) in the 2nd premolar region in patients with tooth loss,respectively.There were significant statistical differences in loss of tooth(P<0.05).Analyzing of spatial relationships among maxillary canine fossa and implants of different sagittal skeletal patterns patients especially class Ⅲ patients,by using CBCT has important significance in guiding dental implants.

Aim To investigate the antidepressant effect of imperatorin(IMP) and explore its mechanism. Methods The model of depression was established by prenatal stress (PS) on late pregnant mother, then male offspring rats were randomly divided into five groups: control, PS model, fluoxetine group(5 mg • kg"1), IMP( 15, 30 mg • kg"1). Sucrose preference test, forced swimming test, and open field test were used to evaluate the behavioral changes. 5- hydroxytryptamine ( 5-HT ) concentration, serotonin transporters(5-HTT) and 5-HT,A receptor(5-HT,AR) mRNA expression in the hippocampus and frontal cortex were measured using an enzyme-linked immunosorbent assay and real-time PCR. Results Compared with PS group, the percentage of sucrose preference and the number of total crossings, center crossings, rearing, grooming and 5-IIT concentration significantly increased in IMP group(/> <0. 05 ,/> <0. 01). The im-mobility time was significantly reduced in IMP group compared with PS group ( P < 0. 01). The 5-HT concentration and 5-HT1A R mRNA expressions of PS offspring rats significantly increased by the administration ofIMP (P<0.05,P<0.01). And, the increased 5- HTT mRNA markedly decreased in IMP group compared with PS group (P < 0. 05 , P < 0. 01 ). Conclusions IMP can obviously improve rat behavior and show anti-depressant effect, which may relate to the 5- HT concentration, 5-HTT and 5-HT,AR mRNA expression in hippocampus and prefrontal cortex.

Objective To survey the status of the clinical competence and meta-cognitive ability of college nursing students, to explore the correlation between the two factors, and analyze the influencing factors of baccalaureate nursing students′ clinical skills, then explore the approach of improving the clinical competence of nursing students. Methods Totally 184 nursing students from Taishan Medical University were investigated by General Information Questionnaire, Clinical Competence Test Scale, University Students Meta Cognitive Ability Scale. Results Nursing students′ average score of meta cognition was 80.48 ± 11.97, the average score of clinical competence was 121.22 ± 22.74. The meta cognitive ability and clinical competence of nursing undergraduate students both maintained at the middle level. Meta cognitive ability was positively correlated with clinical competence. To some extent, enhancing the meta cognitive ability could help students improve the clinical competence. Conclusions To understand the status and correlation between meta cognitive ability and clinical competence can guide students to learn effectively, can help teachers play a good role in teaching effectively.

BACKGROUND:Diabetic lower limb ischemia is prone to involve distal lower limb arteries, and a conventional treatment is often unable to obtain the ideal effect. OBJECTIVE:To investigate the effect and safety of umbilical cord blood stem cel transplantation in the treatment of diabetic lower limb ischemia. METHODS: A diabetic rat model of lower limb ischemia was established, and along the femoral artery, five points
were selected for injection of human umbilical cord mesenchymal stem cel suspension, 20 μL per point. At 1, 2, 4 weeks after transplantation, transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region, and incidence of adverse reactions were recorded. RESULTS AND CONCLUSION:At 1, 2 and 4 weeks after transplantation, the transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region were found increasing, which were significantly different from those before transplantation (P < 0.05). At different time after transplantation, al animals had no inflammatory reactions such as skin bleeding and dermatitis, and local red, sweling, hot, pain, and had no tumor-like growth in organs. These findings indicate that umbilical cord blood stem cel transplantation can safely and significantly improve symptoms of diabetic lower limb ischemia, which has certain application feasibility. Cite this article:Xie LH, Xing L, Zheng H. Feasibility of umbilical cord blood stem cel transplantation for the treatment of diabetic lower limb ischemia. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):78-82.

Objective To analyze the diagnostic and therapeutic process of 3 cases of relapsing polychondritis(RP) and explore strategies for early diagnosis and effective therapy.Methods With retrospective study on three relapsing polychondritis patients in our hospital,we observed their major manifestations at admissions and summarized therapeutic experiences.Results All three patients had symptoms of otalgia,hoarse voice and nasal bridge pit,while their entry causes at admission were anemia,renal function failure and chest pain respectively.Only one of them was insensitive to prednisone but sensitive to methotrexate.Conclusions Because PR being easily misdiagnosed,clinical doctors should improve their caution of diagnostic criteria of RP.Prednisone is the best choice for therapy,but for those insensitive ones methotrexate is also alternative.

Objective To study the characteristics of optical coherence tomography (OCT) in idiopathic macular epiretinal membranes (IMEM) and the relationship between the thickness of fovea and the vision of affected eyes. Methods Total of 67 cases (73 eyes) with clinical diagnosis of IMEM by direct, indirect ophthalmoscopy, three mirror contact lens, fundus color photography or fundus fluorescein angiography(FFA) were examined with OCT. Results Epiretinal membranes(ERMs) with macular edema were found in 32 eyes, proliferative ERMs in 20 eyes, ERMs with macular pseudoholes in 14 eyes and ERMs with laminar macular holes in 7 eyes. Based on OCT, the ERMs were clearly and partially seperated from the retina (27 eyes, 38.36%), the retinal thickness of the fovea was the thickest in proliferative ERMs and the thinnest in ERMs with laminar macular holes. The statistical analysis showed there was a negative correlation between the thickness of fovea and visual acuity (r=-0.454, P= 0.000 ). Conclusion There were four types of image of OCT in IMEM: ERMs with macular edema, proliferative ERMs, ERMs with macular pseudohole and ERMs with laminar macular hole;and the thicker the fovea under the OCT, the poorer the visual acnity in the affected eyes with ERMs.