BACKGROUND:Extracorporeal shock wave (ESW) therapy is using principles of liquid-electric conversion and transfer to produce energy gradient difference and torsional tension in tissues with varied density.It is a matured technique in treating urinary and digestive system calculosis.However,the applications of ESW in treating nonunion or delayed osseous union are few.OBJECTIVE:To explore the therapeutic effects of ESW in treating nonunion or delayed osseous union by animal experiment and clinical verification.METHODS:The effect of ESW on periosteal metabolism was explored by focusing extracorporeal wave on bone tissues,and the autoradiography of H~(3-)TdR labeling rate was observed by animal experiment.ESW was used to treat nonunion patients (n=16) or delayed osseous union patients (n=16),and the follow-uP results were evaluated.RESULTS AND CONCLUSION:By autoradiography,the H~(3-)TdR labeling rate was greater in the experimental sides than that of the control sides at 1 and 2 weeks after operation (P＜0.05-0.01),which confirmed that the mitotic activity of periosteal osteoblasts was increased,namely,endosteal osteogenesis was enhanced.Clinical verification results demonstrated that,at 16 weeks after operation,16 patients with delayed union were all cured,13 were cured among the 16 cases of nonunion,and the remained 3 cases had notably callus formation.No adverse events occurred in all cases.The results suggested that it is reliable to treat nonunion or delayed osseous union using ESW therapy.

Objective To investigate the therapeutic effect of Xiaoxuan Kangfu capsule in treating psoriasis vulgaris. Methods All patients were divided into two groups randomly (60 patients in a control group and 60 patients in a treatment group). In the control group complex Qingdai capsule was given to the patients, with 4 pills for each time and 3 times daily. While in treatment group Xiaoxuan Kangfu capsule was given to the patients with 6 pills for each time and twice daily. 30 days consisted one treatment period for both groups. The patients undertook one to three treatment period according to the patients' pathogenetic condition. The rates of recovery and improvement and were compared. Results In the treatment group, 34 patients were cured (42.5%), and the total effective rate was 87.5%; while in the control group 14 patients were cured (7.5%), and the total effective rate was 72.5%. There was statistic difference between the two groups (P＜0.05). The average heal time of the treatment group was shorter than that of the control group, showing statistic difference (P＜0.05).Conclusion Xiaoxuan Kangfu capsule showed higher recovery rate, shorter healing time and lower recurrence rate in treating psoriasis vulgaris than complex Qingdai capsule. The treatment is deserving clinical spread.

ObjectiveTo identify the chemical composition of stem, leaf and peel ofCynanchumchinese R.Br..MethodsFourier IR spectra, the second derivative spectrum and two-dimensional correlation spectroscopy were adopted to identify IR of different parts ofCynanchumchinese R.Br..ResultsIt was found that the IR spectra of the leaf was similar to that of the peel but was different from the stem. What’s more, in the second derivative spectra of the leaf, it showed that the absorption peak was strong at the position of 1 543 cm-1, 1 515 cm-1, 1 499 cm-1 and 1 467 cm-1, respectively and there existed carbonyl absorption peak at 1 738 cm-1 and 1 659 cm-1 in addition. Therefore, it was inferred that flavonoids were the major components while less in the stem and peel.ConclusionsThe differences of the three parts aboutCynanchumchinese R.Br. were studied through IR spectrum macroscopically, which provided reference for exploring the constituents and clinical medication.

Objective:To make the isolation and 2-DE analysis of mitochondria,in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation,and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable,which provides a basis for the following proteomic study on mitochondria.

Objective:To evaluate the results of treating Perthes disease by pelvic osteotomy and internal fixation with self-reinforced absorbable (SR-PLLA) rods. Methods:50 cases of Perthes disease(53 hips) were involved. According to Herring classification, there were 16 hips of type B ?5 hips of type B/C and 32 hips of type C. All patients were treated by pelvic osteotomy(Chiari)and internal fixation with self-reinforced absorbable (SR-PLLA) rods. Results:All cases were followed up for 2 months to 18 months, with an average of 6 months. All cutting bone was healed. There was no bacterial wound infection or sterile inflammatory foreign-body reaction. Failure of fixation was only occurred in one case. Bone union was seen 6 weeks to 12 weeks, with an average of 8 weeks. All patients restored normal hip joint function and had no pain. Conclusion:This operative technique is simple and can achieve reliable fixation. It can improve bone healing with a reduced healing time and avoid secondary a removal operation. This surgical procedure is an effective way to treat cutting pelvis in Perthes disease.

Objective:To compare characters of urethral healing after different surgical procedures to provide theoretical support for preventing and curing urethral scaring stricture after hypospadias repair.Methods:Hypospadiac animal model was established and three surgical procedures were adopted to repair urethra.The dynamic changes of urethral healing and scar were observed,the expressions of ?-SMactin and TGF-?1 were examined.Results:Mathieu and Onlay island flap(Onlay) had notable collagenous proliferation,which was significantly stronger than that of Tubularized Incised Plate urethroplasty(TIPU)(P

0.05).The positive expression rate of CXCR4 was 96% in cancer cells in regional metastasized lymph nodes.And its expression was lower than that in primary tumors(P

Objective:To investigate the role of TACE in the mechanism of sciatica due to disc herniation.Methods:Autologous nucleus pulposus was applied to the peripheral of the L4 and L5 nerve roots and DRGs to construst animal model.Then Von Frey filaments and a thermal testing device(RTX-1 type)were used to quantify behavior test.Immunocytochemistry and RT-PCR were used to detect the ex-pression changes of TNF-?and TACE to analyze their correlation with the nerve behavior change.Results:The mRNA expression of TNF-?and TACE increased significantly(P

According to various advantages of telemedicine, its applications expanded. In the information age background, the telemedicine is gradually applied in the ordinary armed officers and soldiers in medical and health care, and has played a more and more important role. This article briefly described the overview of the development of telemedicine, analyzed the telemedicine necessity of health insurance and elaborates the role of telemedicine in health service to the ordinary armed officers and soldiers. The prospects and expectations of telemedicine systems are also given.

Four test methods of HEPA filter such as D.O.P test, sodium flame test, oil mist test and particle counting test,are introduced briefly.Furthermore the numerical relations of the above four methods are analyzed. The differences between efficiency of test and practical efficiency and the aspects needed to be considered when testing filter for bio-protection are discussed at the end.