Urokinase-type plasminogen activator plays an important role in the dissolution of clot and the treatment of thrombosis diseases. Presently, more attention has been paid to urokinase metabolism in human body and the relationship between urokinase fibrolysis effect and its receptor in endothelial cell.Cultured endothelial cell was used in the experiment to react with labelled urokinase in the presence or absence of unlabelled urokinase. It was found that the specific bindind of labelled urokinase with endothelial cell increased with the increasing concentration of labelled urokinase and reached the saturation point from 10 to 32 ?g labelled urokinase/ ml. The maximal binding sites with urokinase per cell were 1.4?10?, and Kd value 7.5? 10~(-12)M, Meanwhile, PMA-treated endothelial cell was used to react with ~(125)I-uPA at the same conditions as discribed above. Compared with the specific binding of DMSO-treated endothelial ceil, the maximal binding sites per PMA-treated cell increased from 1.4?10~6 Per untreated cell to 2.2?10~6, and the Kd value from 7.5?10~(-12)M to 9?10~(-12)M.These results showed that urokinase receptor exists on the surface of endothelial cell, and that the number of receptor per cell could be modulated by PMA. It is possible that the urokinase binding with endothelial cell can play an important role in the very short half-life of urokinase in human body.

The development of equipment techniques of CT is comprehensively reviewed,including detectors, tube and data processing system. The clinical application of CT are analyzed. Several problems are put forward which must be dealt with urgently.

Objective To explore the difference of the curative effect between incision-thread-drawing procedure and traditional incision drainage in the treatment of perianal abscess.Methods A total of 150 patients diagnosed with perianal abscess and treated at the first time from June 2015 to June 2016 in our department were selected as the research object in this experiment,who were divided into the observation group(75 cases) and control group(75 cases) according to the surgical method.Patients in the observation group were performed with incision-thread-drawing procedure,while the control group were treated by traditional incision drainage.The overall treatment effect,operation time,postoperative recovery time,hospital stay,hospital costs,wound healing time and postoperative complications between the two groups were observed and compared.Results The difference of the overall treatment effects between two groups were not statistically significant(P>0.05).The operation time,the postoperative recovery time,the hospitalization time and the cost,the time of wound healing of observation group were significantly better than the ones of the control group,the differences were statistically significant(P<0.05).The recurrence rates of anal fistula,abscess and other complications in patients of observation group were significantly lower than the ones of control group.The differences were statistically significant(P<0.05).Conclusion Compared with traditional incision drainage,the incision-thread-drawing procedure is more effective with less complications and lower recurrence rate,which should be the first choice for the treatment of perianal abscess.

The aim of this study was to obtain the alginate gels which could have proper compressive strength and excellent permeability for cell proliferation and could have more promising potentials in the application of tissue engineering. Through the reaction of the carboxyl of the alginate and the amino of methacrylic acid, methylacrylic was generated into alginate long chain which could be enhanced by the polymerization of double bond under thermal reaction condition. And then alginate gel beads were prepared using the mixture of calcium chloride and barium chloride solution as cationic crosslinker, and the compressive modulus and permeability of the prepared alginate gel beads were investigated. When the ratio of barium ions to calcium ions was 5:5, the compression modulus was 189.7 kPa, and it showed the best permeability for trypsin with molecular weight of 24 kDa and entrapment effect for bovine serum albumin with molecular weight of 67 kDa. Compared to compositions of other ratios, the alginate gel beads made in 5:5 mixture indicated excellent compressive modulus and permeability. These results indicated that the alginate hydrogel beads with the ratio of barium ions to calcium ions being 5:5 have a potential application in tissue engineering as a support material and encapsulating materials in cell culture.
Alginates ; chemistry ; Barium Compounds ; chemistry ; Calcium Chloride ; chemistry ; Cations ; Cell Culture Techniques ; Cell Proliferation ; Chlorides ; chemistry ; Cross-Linking Reagents ; chemistry ; Gels ; chemistry ; Metals ; Polymethacrylic Acids ; chemistry ; Tissue Engineering

Autophagy is a highly conservative biological behavior in eukaryotic cells .This dynamic process involves wrappingcytoplasmic components and combining with lysosomes in cells for catabolism .Auto-phagy can not only take part in maintaining homeostasis ,but also be closely related with tumor development and resistant.Therefore,autophagy is a potential target for antitumor drug resistant .Specific inhibition of autophagy in cancer cells combined with chemotherapy is expected to be an effective cancer treatment strategy .

Objective To propose a decision tree-based early warning model so as to predict the medical equipment quantity and quality under special conditions to quantize maintenance and detection staffs allocation,components supply,emergency planning and etc.Methods A data set was established based on the verification report,data on performance parameters detection and daily management log,and then underwent pretreatment.A decision tree came into being with the algorithms of ID3,CHAID and etc.The data rule corresponding to the decision tree was transformed into a series of early warning information.The model was verified by applying it to medical equipment usage management.Results The decision tree developed was applied to analyzing a vehicle-mounted water purifying device maintenance and usage records in some logistics support vehicle,and it's found the main factors contributing to the failures included migration distance along non-paved road,ambient temperature and service time.Conclusion The decision tree-based early warning model for medical equipment quantity and quality gains high feasibility and practical values.

Molecular technologies are sensitive, fast, and cheap in the study on microbial ecology. However, these methods do not provide information about morphology, number, and spatial distribution of the microorganisms. In contrast, Fluorescence in situ hybridization (FISH) combines the precision of molecular biology with the visual information from microscope, and permits visualization and identification of individual miciobial cells within their natural habitat. FISH not only allows the detection of slow growing microorganisms, but also of yet-to-be cultured. It is useful for many applications in diagnosis and assessment of the population structure of complex microbial communities, and is a powerful tool for molecular ecology studies in microbiology, In this paper, major techniques and progresses of FTSH were described. Its application in microbial ecology, as well as problems , pitfalls, and perspectives of FISH are discussed.

In general,the students in Chinese medical universities have technophobia in nuclear medicine experiment,which gives reduction to the cognition and learning of the students.Based on the analysis of the students' psychology and learning,special countermeasures in teaching,therefore,should be used in the conquest of the students' psychological obstacle in order to improve teaching quality of nuclear medicine experiment.

Objective To compare the advantages and disadvantages of the four DNA extraction methods according to the endophytic diversity in the roots, stems, and leaves of mulberry analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) , and by taking the yield, purity and PCR amplification as indexes. Methods Four common methods, i.e., cetyltrimethyl ammonium bromide ( CTAB) , sterile phosphate buffered saline (SPBS) vibration, liquid nitrogen grinding (LNG) , and KIT methods, were used to extract the total DNA from different tissues of mulberry, and then were compared based on the diversity analysis results for endophyte by PCR-DGGE. Results From the roots and stems of mulberry, we got the highest concentration of DNA by LNG extraction method, and got the lowest concentration by SPBS extraction method. But for the leaves of mulberry, the results of the four extraction methods were completely opposite to those for the roots and stems. For different tissues of mulberry, the purity of DNA extracted by KIT method was the best. According to the endophytic bacteria diversity analyzed by 16S rDNA PCR-DGGE, the appropriate method for extraction of DNA was LNG or CTAB, but was not KIT. And according to the results of endophytic fungi diversity analyzed by ITS PCR-DGGE, the best extraction method was KIT, and the unsuited methods varied from the tissues of mulberry. Conclusion The optimum DNA extraction method for mulberry varies from the tissues of mulberry and endophtic bacteria.

Objective To study the value of spiral CT in diagnosing renal cell carcinoma(RCC) and to evaluate the correlation of CT findings with histopathologie features in RCC. Methods 279 cases with RCC proven by surgery and pathology underwent plain and contrast-enhanced CT examinations. The clinic characteristics and CT findings were analyzed statistically and compared with surgical results. Results 90.32% lesions showed medium or obvious enhancement on contrast-enhanced CT,with the exception of granule cells for enhanced uniform, the other cell type of RCC showed heterogeneous enhancement. 32.26% of tumors was of more or less short-burres (χ~2 = 38.2,P<0.01) ,and the there was significant relationship between pathological signs of short burr and the renal capsule involved by tumors. CT qualitative diagnostic rate was 91.40%. CT staging and pathologic staging were of the correla-tive coefficient of 0. 84, while there was not obviously correlation between CT classification of RCC and cancer cell types(P>0.05). Conclusion Spiral CT can better show characteristics of RCC, that can improve the diagnostic accuracy and staging aility for RCC.