Objective to investigate the situation of occupational health of nurses,so as to provide a reference for protecting the health of nurses. Methods Cluster sampling method was employed in this study. totally 1 204 nurses of two three-level general hospital and a cancer hospital in Bei-jing were randomly surveyed with homemade health of nurses′cross-sectional questionnaire,and a nurse medical examination report was access at the same time. Results there was statistically significant difference in the incidence of nurses work pressure and work fatigue in different hospitals and different departments(P < 0.05). 95.18% of the nurses undergo regular medical examinations,of which 56.97% were found abnormal. the dif-ferences in the incidence rate of abnormal medical examination results in nurses were statistically significant among different hospital and different de-partments(P < 0.05). Conclusion Great job stress and work fatigue are observed in nurses,and they have health problems. It is necessary for the hospital to take appropriate interventions to improve physical and mental health nurses.

Carcinoma, Renal Cell ; pathology ; Diagnosis, Differential ; Digestive System Neoplasms ; pathology ; Female ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Kidney Neoplasms ; pathology ; Leiomyoma ; pathology ; Male ; Melanoma ; pathology ; Perivascular Epithelioid Cell Neoplasms ; pathology ; Sarcoma, Clear Cell ; pathology ; Sarcoma, Endometrial Stromal ; pathology ; Skin Neoplasms ; pathology ; Soft Tissue Neoplasms ; pathology ; Uterine Neoplasms ; pathology

Acupuncture Points ; Aged ; Aged, 80 and over ; Bloodletting ; Female ; Humans ; Middle Aged ; Pruritus ; therapy

Adolescent ; Adult ; Breast Neoplasms ; pathology ; Carcinoma ; pathology ; Diagnosis, Differential ; Endothelium, Vascular ; pathology ; Fasciitis ; pathology ; Female ; Hemangiosarcoma ; pathology ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Hyperplasia ; pathology ; Leiomyoma ; pathology ; Leiomyosarcoma ; pathology ; Middle Aged ; Soft Tissue Neoplasms ; pathology ; Uterine Neoplasms ; pathology ; Vascular Diseases ; pathology

Cell Differentiation ; Child ; Dermatofibrosarcoma ; metabolism ; pathology ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Infant ; Lipoblastoma ; metabolism ; pathology ; Liver Neoplasms ; metabolism ; pathology ; Mesenchymoma ; metabolism ; pathology ; Neoplasms, Germ Cell and Embryonal ; metabolism ; pathology ; Sarcoma ; metabolism ; pathology ; Skin Neoplasms ; metabolism ; pathology ; Soft Tissue Neoplasms ; metabolism ; pathology

Aconitum ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Kidney Diseases ; drug therapy ; Medicine, Chinese Traditional ; Rhizome

Aim To investigate the influence of the overexpression of CRMP2 on neural cell apoptosis after ischemia reperfusion injury in rats and its possible mechanism. Methods A total of 192 male adult SD rats were divided into four groups: sham group, cere-bral ischemia/reperfusion group( MCAO group) , cere-bral ischemia with blank plasmid control group( MCAO+GFP group ) , cerebral ischemia with CRMP2 eu-karyotic plasmid group ( MCAO + CRMP2/GFP group) . One day after injecting eukaryotic plasmid, the rats were operated for 120-min ischemia through MCA occlusion and reperfused. At 48 h and 1 wk, the expression of CRMP2 , p53 , Caspase-3 , Caspase-8 and BCL2 in brain tissue was tested by RT-PCR and West-ern blot. Apoptotic cells were observed by TUNEL test. TTC staining was use to detect cerebral infarction volume. The neural function of the rats were also eval-uated. Results Compared with the sham group, the expression levels of CRMP2 and BCL2 in MCAO group and MCAO +GFP group were significantly decreased ( P <0. 01 ) , while p53 , Caspase-3 , Caspase-8 and TUNEL positive cells were elevated(P<0. 01). Inter-vention of CRMP2 eukaryotic plasmid resulted in the increased expression of CRMP2 and BCL2 ( P<0. 01 ) and the decreased p53 , Caspase-3 and Caspase-8 ex-pression. In TUNEL test, overexpression of CRMP2 obviously decreased the number of TUNEL positive cells(P<0. 01). The expression of BDNF was upregu-lated after cerebral ischemic injury ( P<0. 01 ) , while overexpression of CRMP2 increased BDNF more signif-icantly ( P <0. 01 ) . TTC staining showed cerebral in-farction Volume of MCAO + CRMP2/GFP group was obviously decreased ( P <0. 01 ) , and neurologic defi-cits were significantly improved ( P <0. 01 ) . Conclu-sion The overexpression of CRMP2 reduces nerve cell apoptosis possibly by regulating the mitochondrial ap-optosis pathway after cerebral ischemia/reperfusion in-jury to protect nervous system.

Objective: To observe the therapeutic effect of intravenous thrombolysis of recombinant streptokinase (r SK) on acute myocardial infarction (AMI) and its safety. Methods: Twenty patients with AMI received r SK for thrombolysis.The reopening rate of infarct related artery,side effect and the fatality rate in hospital were observed. Results: The reopening rate of infarct related artery was 75%. The incidence of slight hemorrhage, fever, low blood pressure,which could be corrected in short time, was 5%, 15%, 15%, respectively. The fatality rate 5 weeks after AMI was 10%. Conclusion: The therapeutic effect of r SK in the thrombolytic therapy of AMI is definite and the safety is fine.

Objective To screen melanocortin 4 receptor (Mc4R) gene mutation by direct DNA sequencing in order to explore the mutation situation of Mc4R gene in simple obese children in Nanjing.Methods One hundred and five simple obese children(obesity group) and 127 healthy children(healthy control group) were examined for mutations of Mc4R gene.Body mass index(BMI)cutoff points for overweight and obesity adopted Chinese children and adolescents,recommended by China Working Group of Obesity,and all children had no other hereditary and metabolic abnormality.Touch-down PCR was performed to amplify the full length Mc4R gene,then direct DNA sequencing was used to analyze the Mc4R gene.The differences of biochemical index levels between obesity group and healthy control group were analyzed ,including alanine transaminase,aspartate transaminas,total protein,albumin,globulin,albumin/globulin,triglyceride,cholesterol,high density lipoprotein,cortisol,insulin and C peptide.Results There were significant differences of biochemical index levels between obesity group and healthy control group,including triglyceride,insulin level after dining 2 h,C peptide and BMI(Pa