OBJECTIVE:To study the antitumor activity and antioxidative action of glycosaminoglycan from scallop skirt(SS-GAG).METHODS:The effects of different concentration of SS-GAG on the proliferation of four tumor cell lines(HeLa,LOVO,A549,and U251)which had been cultured in vitro were observed by MTT.The effect of SS-GAG on mice within 30 d after innoculation of sarcoma 180 was observed.Multi-kind of biochemistry methods including xanthine oxidase method and thiobarbituric acid colorimetric method were used to study the effect of SS-GAG on total antioxidation activity,activity of SOD,and level of MDA in tumor-bearing mice,which were compared with those in control group.RESULTS:As compared with control group,SS-GAG significantly inhibited the growth of HeLa and U251 cell lines,inhibited the growth of LOVO and A549 cell lines to some degree,significantly prolonged the survival time of the mice with S180 ascites tumor,enhanced the total antioxidant activity and the SOD activity and decreased MDA content in serum(P

OBJECTIVETo explore the acupoints selection rules on peptic ulcer treated with acupuncture and moxibustion,so as to provide references for acupoints clinical selecting.

METHODSLiterature on clinical report and clinical study for peptic ulcer treated with acupuncture and moxibustion was retrieved in CNKI, WANFANG, VIP, CBM, PubMed, Web of Science and other databases from January 1, 2003 to August 31, 2014,and acupoints selecting rules were summarized according to syndromes and symptoms.

RESULTSOne hundred and ten articles were acquired, and acupoints the first to the fifth used were Zhongwan (CV 12), Zusanli (ST 36), Weishu (BL 21), Pishu (BL 20) and Neiguan (PC 6). The main acupoints were Zhongwan (CV 12) and Zusanli (ST 36). As to acupoints mainly selected in accordance with different syndromes: (1)Weishu (BL 21), Pishu (BL 20), Neiguan (PC 6) were matched for the syndrome of deficiency cold in the spleen and the stomach. (2) Weishu (BL 21), Pishu (BL 20), Neiguan (PC 6), Geshu (BL 17) were combined for the syndrome of qi stagnation and blood stasis. (3) Weishu (BL, 21), Pishu (BL 20), Qimen (LR 14) for the syndrome of qi stagnation induced by liver depression. (4) Neiguan (PC 6) and Taichong (LR 3) for the syndrome of liver qi invading the stomach. (5) Weishu (BL 21) and Pishu (BL 20) for the syndrome of weakness of the spleen and the stomach. (6) Weishu (BL 21), Pishu (BL 20), Neiguan (PC 6), Gongsun (SP 4) for the syndrome of cold in the stomach. (7) Weishu (BL 21), Pishu (BL 20), Neiguan (PC 6), San yinjiao (SP 6) for the syndrome of yin deficiency in the stomach. (8) Weishu (BL 21), Pishu (BL 20), Neiguan (PC 6), Yinlingquan (SP 9), Ganshu (BL 18) for the syndrome of phlegm and dampness stagnation. As for acupoints majorly selected according to different symptoms, (1) Weishu (BL 21), Pishu (BL 20), Neiguan (PC 6), Taichong (LR 3) were combined for acid regurgitation. (2) Weishu (BL 21), Neiguan (PC 6), Tianshu (ST 25), Gongsun (SP 4) were selected for abdominal distension. (3) Weishu (BL 21), Neiguan (PC 6), Liangqiu (ST 34), Gongsun (SP 4) for unbearable stomach pain. (4) Weishu (BL 21), Pishu (BL 20), Neiguan (PC 6), Qihai (CV 6), Gongsun (SP 4) for lacking in strength.

CONCLUSIONWe treating peptic ulcer with acupuncture and moxibustion mainly choose Zhongwan (CV 12) and Zusanli (ST 36), and attach importance to acupoints selection based on syndrome and symptom differentiation.

Objective To explore effect on the proliferation and apoptosis of Hela cells in vitro by using vascular endothelial growth factor-C(VEGF-C) antisense oligonucleotides (ASODN). Methods VEGF-C ASODN was transfected into Hela cells by liposome-mediated; the cells transfected with the oligodeoxynuclecotide (SODN) and saline were used as control groups. The efficiency of transfection was detected by fluorescence microscope. The inhibitive rate of cell growth was detected with MTT methods. Apoptosis and cell cycle were evaluated using FCM. The mRNA expression of VEGF-C was determined by RT-PCR, and the expression of VEGF-C was determined by western blotting. Results VEGF-C ASODN had been transfected into Hela cells by liposome-mediated. The index of apoptosis after transfection 48 h was (19.39±1.81)%. G2/M phase cell increased after transfection, meanwhile the expression of VEGF-C degraded on the level of mRNA and protein (P<0.05). Conclusion Transfeeted with VEGF-C ASODN could down-regulate the expression of VEGF on the level of mRNA and protein, block the cell circle, inhibit cell proliferation and induce apoptosis in Hela cells in vitro.

Objective Elevated plasma homocysteine(Hcy) level has been associated with the increased risk for cardiovascular disease.The level of plasma Hcy is related to methylene tetrahydrofolate reductase(MTHFR) gene C677T polymorphism.The aim of this study is to evaluate the relationships between MTHFR gene C677T polymorphism and hypertension,cardiac structure and function.Methods Genotype of MTHFR gene C677T polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis and decided by electrophoresis and sequencing in 695 patients with essential hypertension and 509 age-matched normal controls.Echocardiography was performed in patients with essential hypertension.Results In hypertension group,the frequencies of TT genotype and T allele were significantly higher than those in control group(26.5% vs 20.6%,48.7% vs 42.4%;P=0.015,0.002).The essential hypertension odds ratio(OR) associated with MTHFR 677T allele was 1.300(95%CI:1.166 to 1.450).Logistic regression analysis indicated that MTHFR gene C677T polymorphism was an independent variable of hypertension(P=0.027).End diastolic dimension(EDD),interventricular septal thickness(IVST),left ventricular posterior wall thickness(LVPWT),left ventricular mass(LVM) and left ventricular mass index(LVMI) were higher in hypertensive patients with T allele than that in those with CC genotype,but all had no statistical significance(P=0.428,0.118,0.064,0.077,0.054).Conclusion The 677T allele in MTHFR gene may be a risk factor for hypertension.Hypertensive patients with MTHFR 677T had a trend of hypertrophy,but the relationship has no statistical significance.The relationship between MTHFR gene C677T polymorphism and left ventricular hypertrophy need to be further studied.

Aged, 80 and over ; Humans ; Laryngeal Diseases ; Male ; Neck ; Pharynx

Adolescent ; Adult ; Breast Neoplasms ; pathology ; Carcinoma ; pathology ; Diagnosis, Differential ; Endothelium, Vascular ; pathology ; Fasciitis ; pathology ; Female ; Hemangiosarcoma ; pathology ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Hyperplasia ; pathology ; Leiomyoma ; pathology ; Leiomyosarcoma ; pathology ; Middle Aged ; Soft Tissue Neoplasms ; pathology ; Uterine Neoplasms ; pathology ; Vascular Diseases ; pathology

Cell Differentiation ; Child ; Dermatofibrosarcoma ; metabolism ; pathology ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Infant ; Lipoblastoma ; metabolism ; pathology ; Liver Neoplasms ; metabolism ; pathology ; Mesenchymoma ; metabolism ; pathology ; Neoplasms, Germ Cell and Embryonal ; metabolism ; pathology ; Sarcoma ; metabolism ; pathology ; Skin Neoplasms ; metabolism ; pathology ; Soft Tissue Neoplasms ; metabolism ; pathology

Objective To explore the best time interval to extubate catheter for the postoperative gastric cancer patients,and provide the basis for early extubation after operation.Methods To collect 108 cases with gastric cancer in Department of Forensic Surgery of Yuncheng Central Hospital from March 2013 to February 2014 with indwelling catheter preoperatively.According to the admission sequence,they were divided into the observation group and the control group with 54 cases in each group,the observation group began clipping catheter in 10-15 hours after operation,extubating catheter within 24 hours after operation;the control group began clipping catheter on the second day after operation,and extubating catheter during 48-72 hours after operation.The time of extubation,urinary tract irritation symptoms,resetting catheters and the incidence of early postoperative ambulation in patients of two groups were observed.Results The incidence of urinary tract irritation was lower in the observation group than that of the control group [3.7％ (2/54) vs 16.7％(9/54)],the difference was statistically significant (x2=4.960,P＜0.05).Early ambulation compliance of the observation group was higher than that of the control group (complete compliance 52 vs.40,partial compliance 2 vs.8,no compliance 0 vs.6),the difference was statistically significant (x2 =10.565,P ＜0.05).Conclusions The method of extubation catheter within 24 hours after gastric canter operation can reduce the incidence of urinary tract irritation and improve early ambulation compliance.

BACKGROUND:Obturation with warm gutta-percha can achieve perfect three-dimensional root canal obturation effect. Due to specific structure of oval canal, particularly in the bypass preparation process, excessive cutting of local tooth tissue may lead to tooth tissue thinning and irregular shape. The separation equipment is the metal with good heat conduction, so the heat caused by the bypassing warm gutta-percha obturation would burn periodontal tissue remains controversial. OBJECTIVE:To investigate the changes of root surface temperature produced by two bypassing warm gutta-percha obturation for the separation instrument in oval canalin vitro by using infrared thermography. METHODS:Forty mandibular first premolars were harvested from oval canals, and were separated using stainless steel 15# K file at apical 3 mm, with the length of 3 mm. The root canal bypassing was prepared using TF nickel-titanium file under operating microscope. The teeth were randomly divided into two groups, with 20 teeth in each group, and were obturated with the Thermafil warm gutter-percha and E&Q plus warm gutta-percha vertical compaction, respectively. An infrared thermoviewer was used to measure the external root surface temperature before and after the obturation. RESULTS AND CONCLUSION:Two warm gutta-percha obturation methods used for separating instruments bypassing in the oval root canal, had both caused the rise of root surface temperature, which was less than 10℃. In the Thermafil group, the temperature changes were 3.2-8.1℃ and the average change was 4.97℃. In the vertical condensation group, the temperature changes were 5.5-9.8℃ and the average change was 7.35℃. There were significant differences in the change of root surface temperature between the two groups (P < 0.05). Thermafil warm gutter-percha obturation would increase the root surface temperature, but cannot damage periodontal tissues. It is more secure than warm gutta-percha vertical condensation.