Child ; Child Behavior ; Child Behavior Disorders ; diagnosis ; Child Development ; Developmental Disabilities ; diagnosis ; Humans ; Infant ; Intellectual Disability ; diagnosis ; Pediatrics ; Severity of Illness Index ; Terminology as Topic

Behavioral Medicine ; Child ; Child Behavior ; Child Behavior Disorders ; diagnosis ; prevention & control ; psychology ; Child Development ; Child Health Services ; trends ; China ; Developmental Disabilities ; diagnosis ; prevention & control ; History, 20th Century ; History, 21st Century ; Humans ; Pediatrics ; history ; trends ; Prospective Studies ; Psychology, Child

Objective:To explore the effects of antisense oligonucleotide(ASODN)of connective tissue growth factor (CTGF)on the apoptosis of human hypertrophic scar fibroblasts(HSF)and the related mechanisms.Methods:The hypertrophic scar fibroblasts were cultured in vitro and were divided into the following 3 groups:CTGF-ASODN-treated group, liposome control group and blank control group.The distribution of CTGF-ASODN in HSF of different phases was observed under fluorescent microscope in 3 groups.Flow cytometer was employed to detect the apoptotic rate of HSF and the expression of Fas.The expression of CTGF,Fas and bcl-2 mRNA in HSF was assessed by RT-PCR.Results:24 h after transfection,large quantity of yellow-green fluorescence was seen in the HSF cytoplasm of CTGF-ASDON group in a scattered or doted manner, but not found in that of the other 2 groups.The apoptosis rate of HSF in CTGF-ASDON group was significantly higher than those in the other 2 groups(P

Attention Deficit Disorder with Hyperactivity ; diagnosis ; Humans

The doctor-patient relationship is always the focus in the society. The relationship seems more complex and sensitive in the pediatric surgical ward because its patients are kids. However, the pediatric surgical residents' ability of doctor-patient communication is lack of systemic and norma-tive training, which is not good for the mutual understanding between doctors and patients. This study explores how to enhance the residents' doctor-patient communication skills from the perspective of awareness, professional knowledge and some specific skills. In the meantime, it discusses the need to train the teachers and set up a regular examination-assessment system.

The development in Chinese child health care demands the communication with the society of education.Bio-education is a new promising subject integrating bio-science with education.It is the evidence-based science that combines biology,neuro-physiology,neuro-psychology,brain science with education to study the educational phenomenon of mankind and its principles.It is a bridge through which bio-medicine communicates with education.This new subject will be helpful to build up the new model of integrating medicine with education.It will accelerate both of the basic and applicable research in children's growth and development,learning ability,social adaptive ability and health improvement.Hence,either the field of bio-medicine or education will thrive on the newly founded subject.

Attention Deficit Disorder with Hyperactivity ; Child ; Child Behavior Disorders ; Child Development Disorders, Pervasive ; Developmental Disabilities ; Humans ; Pediatrics ; education ; methods ; Societies, Medical

AIM:The ultrastructure,the levels of NO - 2/NO - 3 were investigated in the myocardium of rats with non-insulin dependent diabetes mellitus. METHODS: In order to establish a model of non-insulin dependent diabetes mellitus (NIDDM), we injected rats with small dose streptozocoi (iv). Two weeks after the injection, the rats developed impaired glucose tolerance. Then, they were fed with high energy diet for eight weeks to form NIDDM. RESULTS: (1) The ultrastructures of cardiac myocytes of NIDDM rats were changed evidently, with swelled mitochondrion and widened intercalated-disk gap; (2) The levels of NO - 2/NO - 3 in the heart tissue of NIDDM rats were significantly lower than those of normal rats( P

AIM: To study the effect of oligonucleotides containing unmethylated CpG motif (CpG ODN) on mouse bone marrow-derived dendritic cells (BMDCs) in anti-HcaF cytotoxicity. METHODS: BMDCs were stimulated by CpG ODN in combination with tumor antigen (TAg). The expression of CD80 on BMDC surface was analyzed by FCAS. Level of IL-12 (p70) in supernatants of BMDC culture was detected by ELISA. The proliferation of T cells was examined by MTT assay. Cytotoxicity of CTL induced by CpG ODN combining with TAg was detected by MTT assay. RESULTS: CpG ODN combining or not combining with TAg up-regulated the expression of CD80 on BMDC surface and stimulated BMDCs to produce a high level of IL-12. CpG ODN-activated BMDC promoted the proliferation of T cells. CTL induced by CpG ODN in combination with TAg appeared strong specific cytotoxicity on Hca-F cells. CONCLUSION: CpG ODN may effectively induce the functional maturation of mouse BMDC in vitro. CpG ODN in combination with TAg can enhance the anti-HcaF cytotoxicity of CTL. [

Objective To investigate the protective effect and mechanism of betaxolol on optic nerves after experimental retinal ischemia-reperfused injury. Methods Retinal ischemia was induced in SD rats by increasing intraocular pressure through intracameral infusion. Sixty-eight rats were randomly divided into 3 groups: normal control (eight rats), 0.25% betaxolol treatment (thirty rats) and saline control group (thirty rats). The latter two groups were subdivided into group 1 day, 3 and 7 days after reperfusion, respectively, with 10 rats in each group. Betaxolol and normal saline was applied to the right eyes of the rats in the treatment group and to the ones in normal saline control group, respectively. The amplitude of b-wave of electron retinograph (ERG) was observed and the histological and ultrastructural changes were detected by light and electron microscopy. The expression of neural nitrogen oxide synthase (nNOS) was detected by immunohistochemistry. The content of malonyldialdehyde (MDA) and the superoxide dismutase (SOD) activity were measured by spectrophotometer. Results [WTBZ]Began from the first day after reperfusion, in saline control group, the amplitudes of ERG b-wave reduced continuously, the histopathological damages of retina were aggravating, the expression of nNOS increased, MDA level increased and SOD level decreased persistently, which significantly differed from the normal control group (P0.01). Conclusion Betaxolol, by reducing intracellular overfreight of Ca~(2+),inhibiting production of NO and elevating the ability of anti-oxidation in rat retina, can protect retinal neurons from ischemia-reperfused injury.