Objective To observe and analyze the clinical effect of laparoscopic assisted vaginal hysterectomy and vaginal hysterectomy for non prolapse of uterus.Methods From March 2015 to April 2016,126 cases of uterus benign lesions received surgical treatment in our hospital were selected and divided into observation group and control group.The observation group was given laparoscopic assisted vaginal hysterectomy resection,while the control group was given routine vaginal hysterectomy.The blood loss,operative time,hospitalization time and complications incidence were compared between two groups.Results The operation time and the amount of bleeding in the observation group were significantly higher than that in the control group,the difference was statistically significant (P ＜ 0.05).The length of stay in the observation group was significantly shorter than that in the control group,the difference was statistically significant (P ＜ 0.05).There was no significant difference in the incidence of surgical complications between the two groups (P ＞ 0.05).The quality of life of the two groups was compared with the total score,the observation group was higher (230.79 + 9.54),P ＜ 0.05,the difference was statistically significant.Concltsion Laparoscopic assisted vaginal hysterectomy and vaginal hysterectomy in the treatment of non prolapse uterus has a good clinical effect,should be chosen and applied based on the actual situation of patients.

Quantum dots( QDs) as a new type of nanostructured luminescent materials have been widely used in biology, materials science, and physical optics. QD-based biosensors can rapidly and accurately detect biological macromolecules or inorganic molecules both in vivo and in vitro. The surface of QDs, prior to their biological detection applications, needed to be modified to enhance their fluorescence properties and lower their biological toxicity. Currently, the surface modification technologies mainly include polymer modification, thiol compounds modification, mercapto propionic acid compounds modification and organic groups modification. Various biosensors can be developed by adopting different modification techniques, which have been adopted to locate and track a variety of biological molecules in vivo. Although a large number of literatures have reported the biological applications of QD-based biosensors, rare systematic reviews of surface modification technologies on QDs have been witnessed. In this paper, we reviewed the surface modification technologies of QDs in biosensors and their medical applications.

Objective To investigate the expression situation of thyroid cancer related gene-1 (TC-1),CyclinD1 andβ-catenin in the tissues of non-small cell lung cancer(NSCLC)and their relationship with the clinical pathologic characteristics,to analyze their relationship with the regulation of Wnt/β-catenin signal pathway to provide the basis for studying the role of TC-1 in NSCLC. Methods The expressions of TC-1,CyclinD1 and β-catenin in 48 patients with NSCLC were detected by immunohistochemical SP method and analyzed by combining the clinical pathological features.Results The expression levels of TC-1,CyclinD1 andβ-catenin in the NSCLC tissue were significantly higher than those in the normal lung tissue;in which,the expression of TC-1 in NSCLC tis-sue was associated with lymph node metastasis and TNM stages;the expression ofβ-catenin in NSCLC tissue was related with the pathological types and tissue differentiation degree.Conclusion The expressions of TC-1,CyclinD1 andβ-catenin show the up-regu-lating trend in NSCLC and may play an important role in the development of lung cancer;TC-1 may be involved in the regulation of Wnt/β-catenin signaling pathway,which provide the new research thought of the NSCLC targeted therapy.

Parathyroid hormone ( PTH) is an important hormone secreted by parathyroid cells , and regulates the metabolism of calcium and phosphorus in the body .In recent years , the toxic effect of PTH on myocardium has been re-ported.Calcium-sensing receptor (CaSR), a member of G protein-coupled receptor family, can feel the subtle change of extracellular calcium concentration and regulate intracellular calcium concentration through multifarious ways in order to control the secretion of PTH .The expression of CaSR is observed in parathyroid cells , renal tubular epithelial cells , myo-cardial cells, etc.Intracellular calcium, as a second messenger, participates in various cell functions , such as excitation-contraction coupling , fertilization and so on .The injury of myocardial cells is intimately linked with high concentrations of PTH and intracellular calcium , and high expression of CaSR .

To explore the relationship between HPRE mutations and noncytolytic anti-HBV infection, the objective eukaryotic expression vectors were constructed by molecular cloning and PCR-based site-directed mutagensis in vitro, and identification was performed using PCR and sequencing analysis. The results showed that eukaryotic expression vectors containing HPRE segment and mutating point were constructed successfully as confirmed by sequencing analysis. The activity of CAT gene obviously increased in the T to C mutation at nt 1504 of HPRE and no alteration in the C to T(G) at nt 1508. The mutation at nt 1508 of HPRE may escape the suppression role of IFN-?on HPRE. These results suggested that the mutation of HPRE might be affected the function of HPRE and influence the regulative function of IFN-? on HPRE, but not of 1FN-? nor of TNF-?.

Animals ; Burns ; immunology ; Humans

Animals ; Burns ; immunology ; Mice

Adult ; Epistaxis ; therapy ; Humans ; Male ; Medicine, Chinese Traditional ; Phlebotomy ; methods ; Young Adult

Objective To explore diagnosis, treatment and prevention of remnant gallbladder from laparoscopic cholecystectomy (LC). Methods A retrospective analysis was performed for the clinical data of 12 patients treated in our hospital for remnant gallbladder caused by LC from December 1994 to December 2007. Results The occurrent rate of the remnant gallbladder in LC was 0.46%(12/2609). All the 12 patients with remnant gallbladder received surgical operations and 3 of them had remnant gallbladder with stones. Remnant gallbladders in the 12 cases were removed by surgical operations and were cured. Conclusion Ultrosonic or MRI examination is preferable and surgery is the effective treatment for this disease. For avoiding the occurrence of the remnant gallbladder in LC,it is essential to adhere to indications of LC before operation and standards of the surgical procedures.

Objective To discuss the influence of drug compatibility on the components of the volatile oil in Flos Lonicerae and Fructus Forsythiae. Methods GC-MS system was used to analyze the volatile oil in Flos Lonicerae, Fructas Forsythiae and their combination. The effect of compatibility on the components and content of volatile oil was evaluated. Results The results of GC-MS showed that gerani]o, tetradecanoic acid methyl ester, hexadecanoic acid ethyl ester, fluoranthene, 9, 12, 15-Octadeeatrienoic acid ethyl ester and linoleic acid was detectable in Flos Lonicerae but was undetectable in the drug pair; camphene, alpha-terpinene, perillaalcohol, 2-beta-pinene, beta-hexadecanal, phellandrene was de-tectable in Fructus Forsythiae but was undetectable in the drug pair; limonene, isolongifolene, nonadecane was detectable in the drug pair but was undetectable in the two single drugs. Conclusion The compatibility of the drug pair has an effect on components and contents in the volatile oil of Flos Lonicerae and Fructus Forsythiae.