2.Determination of Nitrate-N in Water by 2-Isopropyl-5-methyphenol Spectrophotometry
Shiping SUN ; Darong XING ; Lan ZHANG
Journal of Environment and Health 1992;0(04):-
Objective To develop a rapid determination method of nitrate-N in water.Methods In concentrated sulfuric acid,2-isopropyl-5-methyphenol reacts on nitrate and produces the nitro-compound which may turn into the yellow compound in the alkaline solution for the moleculars rearrange.The content of nitrate-N in water can be calculated according to the absorption value at the wave length of 415 nm.Results A good linear relation was found between the concentrations of nitrate-N and the absorption values in the range of 0-15.0 mg/L.The lowest determination concentration was 0.25 mg/L,the recovery rates were 96%-108% and RSD was 1.6%-4.0%.The results were consistent with those determined by UV spectrophotometry.Conclusion The method was simple,sensitive,rapid and accurate.
3.Determination of Sulfate in Water by Barium Chromate Spectrophotometry
Darong XING ; Shiping SUN ; Lan ZHANG
Journal of Environment and Health 2007;0(12):-
Objective To establish a rapid determination method for sulfate in water. Methods In the weak acid solution, sulfate connected with barium chromate into sulfate barium and chromate. Surplus barium chromate precipitated when adding the mixture of CaCl2 and NH3?H2O. The chromate replaced by sulfate was detected at wave length of 420 nm. Results The RSDs were 1.8%-6.8% and the recovery rates were 98.9%-101.0%. Conclusion The method was rapid, simple and its sensitivity and accuracy can meet the requirement of standard examination methods for drinking water.
4.Expression of ?-amyloid protein after reperfusion of transiently Incomplete forebrain Ischemia in rat hippocampus
Ang XING ; Naidong WANG ; Lan TAN
Chinese Journal of Neurology 2001;0(01):-
Objective To elucidate the delayed neuron death and the expression of ?-amyloid protein (A?) in hippocampus after reperfusion of transiently ischemic lesion.Methods Immunohistochemical and HE technique was used to examine the delayed neuron death and expression of ?-amyloid protein at 6 h,2 d,3 d,7 d,14 d,35 d after reperfusion of transiently incomplete forebrain ischemia in rat hippocampus.Results Delayed neuron death was seen at 3 days after ischemia/reperfusin in CA 1 area of the hippocampus. A? immunoreactivity began enhanced at 2 d (A:0.082?0.011)after reperfusion, up to peak at 7 d(A:0.175?0.024), disapeared at 35 d.Conclusion Delayed neuron death was occurred after reperfusion of transiently incomplete forebrain ischemia in rat hippocampus,and at the same time, A? was up expression, which is suggested to be an important role of A? in neuron death after reperfusion of ischemic lesion.
6.Clinical Observation of Shenxiong Glucose Injection in the Treatment of Chronic Renal Failure
Xing FAN ; Lihua ZHONG ; Tianbiao LAN ;
Chinese Journal of Pharmacoepidemiology 2007;0(03):-
Objective:To evaluate the clinical efficacy of shenxiong glucose injection in the treatment of chronic re- nal failure(CRF).Method:78 patients with CRF were divided into a treatment group(n=40)and a controlled gronp(n= 38).The treatment group received shenxiong glucose injection 200ml daily,14 days for one course of treatment.The con- trolled group received ligustrazine injection 80mg with 5% glucose.The changes of serum BUN and SCr were observed.Re- sult:The serum BUN and SCr were significantly decreased in the two groups after the treatment(P
7.Comparison of two criteria for evaluating severity of community-acquired pneumonia in China
Chinese Journal of General Practitioners 2005;0(08):-
Objectives To understand current status of the admission and treatment for the patients with community-acquired pneumonia (CAP) in central hospitals of Shanghai area,and to evaluate the severity of patients admitted to the hospital with CAP by the criteria set in the Guidelines for Diagnosis and Treatment for CAP developed by the Chinese Medical Association in 2006 and provide evidence for its popularization and application throughout the country.Methods Medical records of 137 patients with CAP admitted to the hospital from January 1,2005 to September 30,2006 were retrospectively studied and analyzed with SPSS 10.0 software.Chi-square test and ANOVA were used to evaluate the severity of the patients with CAP by the criteria set in the Guidelines and to correlate it with pneumonia severity index (PSI).Statistical analysis was performed for the difference between length of hospitalization,cost,length of intravenous use of antibiotics,the number of risk factors,and fatality during hospitalization between three groups of patients categorized based on the severity criteria in the Guidelines.Results There existed a good relationship between the criteria for severity of CAP by the Guidelines and PSI,with a Pearson's coefficient of correlation of 0.577,P
8.Therapeutic Effect of Benazepril Combination with Valsartan in Treatment of Chronic Kidney Patients with Proteinuria and Hypertension
Xing FAN ; Tianbiao LAN ; Xu WANG
Chinese Journal of Pharmacoepidemiology 2007;0(05):-
Objective:To evaluate the clinical efficacy of benazepril in combination with valsartan in treating chronic kidney patients with proteinuria and hypertension.Method:75 chronic kidney patients were divided into benazepril group(n=28)and valsartan group(n=26)and combination treatment group(n=21).The changes of 24 hours urinary protein excretion and blood pressure and serum BUN and SCr were observed before the treatment and 4 and 8 weeks after it. Result:The 24 hours urinary protein excretion and blood pressure in all the groups showed significant decreases after the treatment(P0.05),but the levels of SUA were significantly lower in the valsartan group and combination treatment group than those in the benazepril group(P
10.Effects of Shen-wu Capsule on learning and memory ability and brain β-amyloid content in APP transgenic mice
Ying XING ; Lan ZHANG ; Lin LI
Chinese Journal of Rehabilitation Theory and Practice 2005;11(5):324-326
ObjectiveTo investigate the effects of Shen-wu capsule on learning and memory ability and its mechanism in APP transgenic mice. MethodsThe APP 695V717I transgenic mice were randomly divided into model group, Shen-wu low dose group (0.4g/kg·d) and high dose group (1.2g/kg·d). Normal control adopted the same age and background C57BL/6J mice. The animals were administered intragastrically by the drug or water from 4 month old to 10 month old. Morris water maze and object recognition test were performed to measure the learning and memory ability. The content of β-amyloid (Aβ) in the brain cortex homogenate was detected with RIA,and amyloid plaques were measured with Congo red staining. ResultsIn the Morris water maze test, swimming time and swimming distance of model group were prolonged distinctly(P<0.01). Shen-wu high dose group obviously shortened swimming time(P<0.05). In the object recognition test, the relative time to the new objection in model group was obviously shorter than that in the control group(P<0.05). The relative time to the new objection for Shen-wu high dose group was obviously longer than the model group(P<0.05). The content of soluble Aβ in model group was higher than that of the control group(P<0.05). Shen-wu group decreased the soluble Aβ distinctly(P<0.01). The amyloid plaques were increased in the brain of model mice(P<0.01). Each group of Shen-wu decreased amyloid plaques significantly(P<0.01).ConclusionShen-wu Capsule ameliorated the learning and memory function disorder and decreased Aβ formation in the brain of APP transgenic model mice.