Significant progress has been made toward understanding the tumorigenic role and clinical significance of the T1799A B-type Raf kinase(BRAF)mutation in papillary thyroid cancer(PTC). With these advances, it has become clearer that BRAF mutation will likely have significant impact on the clinical management.

Many researches proved that inflammation plays a decisive role in occurrence, development and prognosis of acute coronary syndrome (ACS).Atorvastatin possesses many other effects besides lipid regulation, and anti-inflammatory effect is the most significant one.Some studies indicated that atorvastatin can reduce levels of high sensitive C reactive protein (hsCRP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α in ACS patients, then inhibit postoperative inflammation, improve postoperative blood flow perfusion and reduce occurrence of acute coronary events.The present article made a review on influence of atorvastatin on levels of hsCRP, IL-6 and TNF-α in ACS patients.

Objective To investigate the effects and mechanism of Shufeng Decoction resisting type I hypersensitivity. Methods Type I hypersensitivity was induced by OVA. The content of IL-4, IFN-? and IgE in serum of the rats treated by Shufeng Decoction were determined by ELISA. Results Shufeng Decoction can increase the content of IFN-? in model rats (P0.05). IFN-?/IL-4 of Shufeng Decoction was significant higher than model group (P0.05). Conclusion The effects and mechanism of Shufeng Decoction on prophylaxis and treatment of type I hypersensitivity of skin (acute urticaria) may be related with regulating cell factors and influencing the balance of Th1/Th2.

Objective To investigate the effects and mechanism of Xiaomin granules on type Ⅰ hypersensitivity.Methods The experimental animal model was made for type Ⅰ hypersensitivity.The mice were divided into five groups named vacant control group,Xiaomin granules(low-,middle-and high-dose) group,chlorpheniramine group randomly and were given homologous medication respectively.Results Xiaomin granules can inhibit capillary penetrance caused by histamine and 5-HT in mice(P 0.05).Xiaomin granules could inhibit the mice hind paw edema degree caused by histamine.The further study found that Xiaomin granules could decrease anaphylactic shock of the mice and decrease the death rate.Conclusions Xiaomin granules could restrain type Ⅰ hypersensitivity by inhibiting inflammatory mediators.

BACKGROUND:The thickness of ceramic materials plays a crucial role in the reappearance of veneer restorations. Due to the limited space for the construction of porcelain veneer, it is difficult to cover the abutment tooth color, and the final color after restorations is achieved through the overlapping of prosthetic restorations color and abutment tooth color. In recent years, there is little evidence on the effect of veneer materials on the color. OBJECTIVE:To evaluate the color matching of porcelain veneer restorations with different ceramic materials to shade tab. METHODS:Fifteen veneer restorations were fabricated by three ceramic materials (VITA Mark II, Ivoclar E.max LTCAD and Ivoclar E.max CAD Multi, A2 shade). Veneer restorations were controled at the thickness of 0.6 mm. The color parameters of veneer restorations were measured by Olympus Crystaleye against the natural color shade resin material substrate. The color differences were calculated between veneer restoration and shade tab (A2 shade) at the cervical, central and incisal sections, respectively. The color differences (?E values) were statisticaly analyzed using a one-way analysis of variance. RESULTS AND CONCLUSION:The color value of E.max CAD LT block was the closest one among the three blocks to the standard shade guide. The results of one-way analysis of variance for the ?E values showed that, there were significant differences between the cervical sections of the veneer restorations and the shade guide (P< 0.05). At the cervical sections, the ?E values between three kinds of blocks with the standard shade guide showed significant differences (P < 0.05); the differences were also significant in L values at the central and incisal sections, a values at the incisal sections and b value at the shoulder sections (P < 0.05). Our findings indicate that, there are significant differences in the color of porcelain veneer restorations with different ceramic materials to shade tab. The difference is also found among the three sections of the veneer restorations. In the clinical application, the final prosthesis color effects should be noted, which can change by surface staining and bonding technology.

Objective To systematically assess the relationship between oral administration of aspirin and survival benefit for patients with colorec?tal cancer(CRC)by meta?analysis. Methods Relevant studies were identified through searching PubMed and EMBASE. Random?effects model was derived to composite the pooled hazard ratio for overall mortality and CRC?specific mortality. The subgroup analysis was conducted for included data,and the bias analysis was reported. Results Thirteen studies on aspirin therapy were finally included in this meta?analysis. The overall surviv?al benefit associated with oral administration of aspirin represented an HR of 0.83(95%CI:0.71?0.96). Oral administration of aspirin was also asso?ciated with CRC?specific survival(HR=0.77,95%CI:0.64?0.93). No evidence was observed of an association between prediagnostic aspirin use and CRC overall survival(HR=1.00,95%CI:0.85?1.18)or CRC?specific survival(HR=0.97,95%CI:0.83?1.13). Conclusion These findings provide further indication that post?diagnostic aspirin therapy can improve CRC prognosis.

Objective To investigate the effect of liver phosphatidylinosital 3-kinase/protein kinase B (PI3-K/AKT) pathway on the decrease of insulin sensitivity in fetal growth restriction (FGR) rats.Methods Twenty pregnant female rats were randomly divided into two groups one day after conception:normal-protein group and low protein group (n=10,respectively).Rats in low-protein group was given low protein diet (8.00％ protein) during pregnancy to build FGR model,while normal-protein group was given normal protein diet (20.00％ protein).On day 3,7,14,30,60 and 90 after birth,fasting blood samples of 8 male FGR offsprings from low-protein group and 8 normal offsprings from normal-protein group were collected to measure fasting plasma glucose and insulin level.Then insulin resistance index and insulin sensitivity index were calculated to determine insulin sensitivity.On day 7,14,30,60 and 90 after birth,liver tissue of 8 male FGR and normal offsprings were collected,insulin receptor substrate 1,2 (IRS1/IRS2)and glucose transporter 4 (GLUT4) mRNA expression were measured by real-time fluorescence polymerase chain reaction and the protein expressions of IRS1,PI3-K (subunit p110β),and AKT and phosphorylated AKT (pAKT) were measured by Western blot.The relationships between the expression changes of key molecules of PI3-K/AKT pathway and insulin sensitivity were analyzed by correlation and multiple linear regression method.Results (1) Mean birth weight of baby rats in low-protein group was significantly lower than that of normal-protein group [(4.92±0.36) g vs (6.43±0.59) g,t=14.73,P＜0.05].The incidence of FGR in low-protein group was 88.2％ (97/110); and for male offsprings,it was 94.1 ％ (48/51).(2) Compared to normal offsprings,fasting plasma glucose levels of male FGR offsprings were significantly higher from the age of 60 days to 90 days.Insulin levels and insulin resistance index were significantly higher and insulin sensitivity index was lower from the age of 30 days to 90 days,P＜0.05 respectively.(3) Compared to normal offsprings,IRS1 (0.45 ± 0.02 vs 0.68± 0.03,t=16.633,P＜0.05) and IRS2 mRNA (0.34±0.10 vs 0.70±0.19,t=4.864,P＜0.05) expressions in FGR offsprings were lower from day 7 after birth to day 90 (0.48±0.03 vs 0.59±0.05,t=5.237,P＜0.05; 0.49±0.20 vs 0.70±0.11,t=2.253,P＜0.05).There were no differences in expressions of GIUT4 mRNA and AKT protein between two groups (P＞ 0.05).IRS1,PI3-K and pAKT protein expressions of FGR offsprings decreased significantly from day 14 (0.22±0.05 vs 0.52±0.11,t=7.024,P＜0.05; 0.46±0.03 vs 0.97±0.08,t=17.508,P＜0.05; 0.62±0.10 vs 0.89±0.08,t=6.100,P＜0.05) to day 90 (1.11±0.08 vs 1.32±0.14,t=3.714,P＜0.05; 0.63±0.07 vs 1.00±0.19,t=5.206,P＜0.05;0.28±0.03 vs 0.45±0.10,t=4.880,P＜0.05).(4) The pAKT protein expression level of FGR rats was positively correlated with insulin sensitivity index (r=0.704,P＜0.05) ; while negatively correlated to the level of fasting plasma glucose (r=-0.609,P＜0.05),fasting insulin (r=-0.561,P＜0.05) and insulin resistance index (r =0.577,P＜ 0.05).Conclusions The changes of some key molecules' expressions of PI3-K/AKT pathway in liver might be involved in the insulin resistance in FGR rats.

Objective To evaluate the change of renal function in patients with acute stroke by estimate glomerular filtration rate (e-GFR). Methods One hundred patients with acute stroke admitted into hospital from emergency department in 72 h were enrolled. There were 100 patients, with 50 male patients and 50 female patients. Fifty-eight patients were older than 50 years old, and 42 patients were less than 50 years. Blood samples were collected from the next day in hospital to detect the level of creatinine (Cr), then e-GFR was calculated with a simplified method of modification of diet in renal disease. According to the National Kidney Foundation published kidney disease outcome quality initiative (K/DOQI), the renal function of these 100 patients was estimated. The outcome of e-GFR and Cr were compared. The differences of e-GFR in patients with acute stroke were analyzed from the aspects of age and gender. Results 56.0% (56/100) patients were determined as decreased renal function according to the standard of GFR<90 ml/(min · 1.73 m2). 4.0% (4/100) patients were determined as decreased renal function according to the standard of Cr> 124μmol/L. The abnormal rate of e-GFR in patients older than 50 years old was 93.1%(54/58), in patients less than 50 years old was 4.8%(2/42), and there was significantly difference (χ2=77.16, P<0.01). The abnormal rate of e-GFR in male and female had no significantly difference: 54.0%(27/50) vs. 58.0% (29/50), P > 0.05. Conclusions The decreased renal function in patients with acute stroke is common. The detection of e-GFR can more accurately assess the patient′s renal function and provide a basis for individual choice of drugs.

Objective: To select the prescription of Harmine HCl ointment. Methods: The orthogonal design was used for selecting the prescription with transdermal absorption rate constant (K) and flow energy of activition (E ?) as selecting standard. Results: The optimum prescription is as follows: Azone (2.0%), Span-80 ( 0.2%), Tweens-80 (0.4%), Glycerylmonostearate (2.5%), Vaselin (4.0%), Liquid (11%). Conclusion: The prescription design is available, and the ointment has a good stability.

Objective To explore the molecular effect and interaction among nephrin, podocin, CD2AP and ?-actinin-4. Methods Firstly, the recombinant RNA interference (RNAi) plasmid-psiRNA-hH1GFPzeo, specifically targeting to the mRNA of nephrin, podocin, CD2AP or ?-actinin-4, was respectively tansfected into the mouse podocyte clone (MPC5) to each knockdown (KD) the expression of nephrin, podocin, CD2AP or ?-actinin-4. Molecular distributions were revealed by confocal microscopy, and the mRNA and protein expressions were detected with semi-quantitative RT-PCR and Western blotting. Results (1)In podocin KD group (siPod966 and siPod54), the mRNAs of podocin and nephrin were not detected, their protein decreased 92% and 79%, 82% and 67%, respectively. The mRNA and protein level of CD2AP increased 62% and 42%, 71% and 46%, respectively, whereas ?-actinin-4 did not change. In nephrin KD group (siNep492), the mRNA expression and protein level of nephrin were not detected, CD2AP increased 35% and 48%, respectively; and whereas podocin and ?-actinin-4 did not change. In CD2AP KD group (siCda744 and siCda21), the mRNA of expression CD2AP was not detected, and its protein level decreased 92% and 83%, the mRNA and protein of nephrin decreased 60% and 48%, 76% and 72%, respectively; podocin increased 38% and 22%, 56% and 44%, respectively; whereas ?-actinin-4 did not change. In ?-actinin-4 KD group (siAct1790 and siAct319), the mRNAs expression of ?-actinin-4 and nephrin decreased 69% and 58%, 64% and 49%, respectively; their protein level decreased 81% and 55%, 71% and 64%, respectively. However, the mRNAs of podocin and CD2AP increased 50% and 34%, 45% and 28%, respectively; and their protein level increased 64% and 46%, 65% and 42%, respectively. (2) With their expression change, the distributions of nephrin, podocin and CD2AP shifted evidently from the cell membrane surface to the nucleus circumference, whereas ?-actinin-4 showed no change, which was still localized in the cytoplasm and further extended to foot processes. Conclusion (1) Nephrin might more independently play a crucial role in the slit diaphragm complex. (2) Alpha-actinin-4 might interact direcdy or indirectly with nephrin, podocin and CD2AP. (3) The relationship among these podocyte molecules might not be spontaneous, either a single-directional or bi-directional reaction. (4) The normal localization of these podocyte molecules might depend on their normal expression quantity.