Significant progress has been made toward understanding the tumorigenic role and clinical significance of the T1799A B-type Raf kinase(BRAF)mutation in papillary thyroid cancer(PTC). With these advances, it has become clearer that BRAF mutation will likely have significant impact on the clinical management.

Many researches proved that inflammation plays a decisive role in occurrence, development and prognosis of acute coronary syndrome (ACS).Atorvastatin possesses many other effects besides lipid regulation, and anti-inflammatory effect is the most significant one.Some studies indicated that atorvastatin can reduce levels of high sensitive C reactive protein (hsCRP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α in ACS patients, then inhibit postoperative inflammation, improve postoperative blood flow perfusion and reduce occurrence of acute coronary events.The present article made a review on influence of atorvastatin on levels of hsCRP, IL-6 and TNF-α in ACS patients.

Objective To investigate the effects and mechanism of Shufeng Decoction resisting type I hypersensitivity. Methods Type I hypersensitivity was induced by OVA. The content of IL-4, IFN-? and IgE in serum of the rats treated by Shufeng Decoction were determined by ELISA. Results Shufeng Decoction can increase the content of IFN-? in model rats (P0.05). IFN-?/IL-4 of Shufeng Decoction was significant higher than model group (P0.05). Conclusion The effects and mechanism of Shufeng Decoction on prophylaxis and treatment of type I hypersensitivity of skin (acute urticaria) may be related with regulating cell factors and influencing the balance of Th1/Th2.

BACKGROUND:The thickness of ceramic materials plays a crucial role in the reappearance of veneer restorations. Due to the limited space for the construction of porcelain veneer, it is difficult to cover the abutment tooth color, and the final color after restorations is achieved through the overlapping of prosthetic restorations color and abutment tooth color. In recent years, there is little evidence on the effect of veneer materials on the color. OBJECTIVE:To evaluate the color matching of porcelain veneer restorations with different ceramic materials to shade tab. METHODS:Fifteen veneer restorations were fabricated by three ceramic materials (VITA Mark II, Ivoclar E.max LTCAD and Ivoclar E.max CAD Multi, A2 shade). Veneer restorations were controled at the thickness of 0.6 mm. The color parameters of veneer restorations were measured by Olympus Crystaleye against the natural color shade resin material substrate. The color differences were calculated between veneer restoration and shade tab (A2 shade) at the cervical, central and incisal sections, respectively. The color differences (?E values) were statisticaly analyzed using a one-way analysis of variance. RESULTS AND CONCLUSION:The color value of E.max CAD LT block was the closest one among the three blocks to the standard shade guide. The results of one-way analysis of variance for the ?E values showed that, there were significant differences between the cervical sections of the veneer restorations and the shade guide (P< 0.05). At the cervical sections, the ?E values between three kinds of blocks with the standard shade guide showed significant differences (P < 0.05); the differences were also significant in L values at the central and incisal sections, a values at the incisal sections and b value at the shoulder sections (P < 0.05). Our findings indicate that, there are significant differences in the color of porcelain veneer restorations with different ceramic materials to shade tab. The difference is also found among the three sections of the veneer restorations. In the clinical application, the final prosthesis color effects should be noted, which can change by surface staining and bonding technology.

Objective To systematically assess the relationship between oral administration of aspirin and survival benefit for patients with colorec?tal cancer(CRC)by meta?analysis. Methods Relevant studies were identified through searching PubMed and EMBASE. Random?effects model was derived to composite the pooled hazard ratio for overall mortality and CRC?specific mortality. The subgroup analysis was conducted for included data,and the bias analysis was reported. Results Thirteen studies on aspirin therapy were finally included in this meta?analysis. The overall surviv?al benefit associated with oral administration of aspirin represented an HR of 0.83(95%CI:0.71?0.96). Oral administration of aspirin was also asso?ciated with CRC?specific survival(HR=0.77,95%CI:0.64?0.93). No evidence was observed of an association between prediagnostic aspirin use and CRC overall survival(HR=1.00,95%CI:0.85?1.18)or CRC?specific survival(HR=0.97,95%CI:0.83?1.13). Conclusion These findings provide further indication that post?diagnostic aspirin therapy can improve CRC prognosis.

Objective To investigate the effects and mechanism of Xiaomin granules on type Ⅰ hypersensitivity.Methods The experimental animal model was made for type Ⅰ hypersensitivity.The mice were divided into five groups named vacant control group,Xiaomin granules(low-,middle-and high-dose) group,chlorpheniramine group randomly and were given homologous medication respectively.Results Xiaomin granules can inhibit capillary penetrance caused by histamine and 5-HT in mice(P 0.05).Xiaomin granules could inhibit the mice hind paw edema degree caused by histamine.The further study found that Xiaomin granules could decrease anaphylactic shock of the mice and decrease the death rate.Conclusions Xiaomin granules could restrain type Ⅰ hypersensitivity by inhibiting inflammatory mediators.

Objective:To explore the hepatocyte insulin sensitivity of intrauterine growth retardation ( IUGR) rats and establish an insulin resistance cell model in vitro.Methods: An IUGR animal model was established by protein malnutrition during the mother pregnancy .On 60 d and 90 d after birth , the offspring rats were fasted for 12 hours and then their angular vein blood was collected to measure the fasting plasma glucose and fasting serum insulin level , then the insulin resistance index ( HOMA-IR) and insulin sensitivity index ( ISI) were calculated .The insulin sensitivity was evaluated by HOMA-IR and ISI.Primary hepatocytes from each group were respectively isolated by two-step perfusion with collage-nase and were defined as normal hepatocytes group and IUGR hepatocytes group .The normal hepatocyte group was divided into two groups: control group and insulin induction group .Insulin induction group was established by primary cultures of normal hepatocyte incubated with varying dilutions of insulin . CCK-8 was used to detect the viability of the cultured hepatocytes .Glucose oxidase-peroxidase method kit was used to measure glucose consumption of the hepatocytes .Results:HOMA-IR was significantly higher in IUGR rats than in the normal rats at the age of 60 days ( t=-17 .02 , P<0 .05 ) and 90 days ( t=-12.52, P<0.05).ISI was significantly lower than in the normal rats aged 60 days (t=5.61, P<0.05) and 90 days (t=12.42, P<0.05).There were no significant differences in hepatocyte viability among the control group , IUGR group and insulin induction group after incubation of 48 h on day 60 (F=1.34, P=0.29) and day 90 (F=0.22, P=0.81).The glucose consumption of the IUGR group and insulin induction group were significantly decreased compared with the control group on day 60 ( F=9.28, P=0.002) and day 90 (F=56.60, P<0.001), while there was no significant difference be-tween the IUGR group and insulin induction group (P=0.08, P=0.10).Conclusion:The insulin sen-sitivity of hepatocytes of IUGR rats decreased from adolescence to adulthood .High-dilution insulin may induce insulin resistance cell model in vitro.

The LEAFY (LFY) homologous gene of Dendrobium moniliforme (L.) Sw. was cloned by new primers which were designed based on the conservative region of known sequences of orchid LEAFY gene. Partial LFY homologous gene was cloned by common PCR, then we got the complete LFY homologous gene Den LFY by Tail-PCR. The complete sequence of DenLFY gene was 3 575 bp which contained three exons and two introns. Using BLAST method, comparison analysis among the exon of LFY homologous gene indicted that the DenLFY gene had high identity with orchids LFY homologous, including the related fragment of PhalLFY (84%) in Phalaenopsis hybrid cultivar, LFY homologous gene in Oncidium (90%) and in other orchid (over 80%). Using MP analysis, Dendrobium is found to be the sister to Oncidium and Phalaenopsis. Homologous analysis demonstrated that the C-terminal amino acids were highly conserved. When the exons and introns were separately considered, exons and the sequence of amino acid were good markers for the function research of DenLFY gene. The second intron can be used in authentication research of Dendrobium based on the length polymorphism between Dendrobium moniliforme and Dendrobium officinale.

Adult ; Aged ; Cardiovascular Agents ; therapeutic use ; Diastole ; drug effects ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Heart Failure ; drug therapy ; physiopathology ; Humans ; Injections ; Male ; Middle Aged ; Phytotherapy ; Systole ; drug effects ; Ventricular Dysfunction, Left ; complications ; drug therapy

Liposome is an artificially prepared spherical vesicle that has a phospholipid bilayer. Given that the basic structure of its biological membrane is also a lipid bilayer membrane, liposome shares similar structures with body cells Therefore, liposome has good biocompatibility and advantages such as biodegradability, low immunogenicity, and subtle toxicity. Liposome has been widely ap-plied as an effective drug carrier. Studies on liposome-encapsulated fluorescent dye on tumor tracing have been reported in recent years. Liposome can become a more advantageous transport carrier with continuous development of surface modification materials and prepa-ration methods. The long cycle, targeted liposome-encapsulated drugs, and fluorescent dye have become the focus of interest for several researchers. This article mainly discusses the application and progress of long cycle and targeted liposome in cancer research.