1.Analysis of the verification results of the laboratory ability to detect ammonia nitrogen in drinking water in the CDC in Hubei Province
Xinfu PENG ; Li CHEN ; Mingxing ZENG ; Dongmei SUN ; Zhaohui ZHENG
Journal of Public Health and Preventive Medicine 2020;31(5):61-63
Objective To understand the ability of the CDC to detect ammonia nitrogen in drinking water in Hubei Province. Methods The blind samples of two concentration levels were tested in the laboratories of the disease control institutions in the province, and the test results were evaluated by double-sample Z score. Results The satisfaction rate was 76.29% (74/97), the problem rate was 15.46% (15/97), and the dissatisfaction rate was 8.25% (8/97). Conclusion The detection ability of ammonia nitrogen in drinking water in laboratories of disease control institutions in Hubei Province is generally high,however,some laboratories need to be further improved.
2.Effects of bamboo leaf flavonoids on liver injury,antioxidant function and related gene expression in rats induced by diquat
Chao WU ; Shuwan LU ; Xueyan SHI ; Caimei YANG ; Xinfu ZENG ; Ruiqiang ZHANG ; Jinsong LIU
Chinese Journal of Veterinary Science 2024;44(7):1498-1506
Bamboo leaf flavonoids(BLF)are compounds extracted from bamboo leaves,possessing properties including antioxidant,antimicrobial and anti-inflammatory properties.This study aimed to investigate the effects of BLF on liver damage,antioxidant function,and related gene expression in rats induced by diquat(DQ).Thirty-two 5-week-old male Sprague-Dawley(SD)rats were randomly divided into four experimental groups:the control group(Con),1 000 mg/kg BLF group(BLF),DQ stress group(DQ),and 1 000 mg/kg BLF+DQ stress group(BLF-DQ).The results showed that compared to the Con,the DQ group exhibited significantly decreased serum AST lev-els(P<0.05),as well as decreased levels of T-AOC,GPX,SOD,and CAT in the liver(P<0.05),and increased MDA levels in rats(P<0.05).Additionally,the gene expression levels of HO-1,GPX,CAT,SOD1,and Nrf2 in the liver were significantly reduced(P<0.05).In contrast,1 000 mg/kg BLF significantly decreased serum AST and ALT levels(P<0.05),increased levels of T-AOC,GPX,CAT,and SOD in liver(P<0.05),and significantly increased gene expression of HO-1,GPX,CAT,SOD1,Nrf2,and NQO1(P<0.05).Compared to the DQ group,BLF-DQ significant-ly decreased liver index(P<0.05),reduced serum AST and ALT levels(P<0.05),increased lev-els of CAT,GPX,and T-AOC in liver(P<0.05),decreased MDA levels(P<0.05),and signifi-cantly upregulated gene expression levels of HO-1,GPX,CAT,SOD1,and Nrf2(P<0.05).These findings indicated that BLF alleviate liver damage caused by DQ stress in rats,improve liver an-tioxidant function inhibition,activate the Nrf2 signaling pathway and PINK/Parkin mitophagy-re-lated gene expression.