OBJECTIVE:To investigate the effect of intravitreal injection of ranibizumab on visual acuity and GAS6,SDF-1 and VEGF levels in serum of patients with proliferative diabetic retinopathy lesions. METHODS:56 patients diagnosed as prolifera-tive diabetic retinopathy in our hospital during Mar. 2012 to Mar. 2014 were analyzed retrospectively and divided into simple lateral eyes group(29 cases)and bilateral eyes group(27 cases). All patients received single intravitreal injection of ranibizumab. The se-rum levels of GAS6,SDF-1 and VEGF were detected before treatment and 1,4 and 8 weeks after treatment. The visual acuity of patients were detected before treatment and 1 week after treatment. RESULTS:1,4 and 8 weeks after treatment,the serum levels of GAS6,SDF-1 and VEGF in 2 groups were decreased significantly,with statistical significance(P<0.05);but there was no sta-tistical significance between 2 groups(P>0.05). 1 week after treatment,the number of letter which had been seen by patient’s BC-VA were increased,compared to before treatment,with statistical significance(P<0.05);but there was no statistical significance between 2 groups(P>0.05). CONCLUSIONS:Intravitreal injection of ranibizumab can improve visual acuity of patients with pro-liferative diabetic retinopathy.

Objective To study the value of serum HMGB1(high mobility group box-1) and carbohydrate antigen 72-4(CA72-4) in patients with early gastric cancer .Methods 50 cases of early gastric cancer patients were enrolled as a group ,45 cases of gastric lesions as benign gastric benign group ,100 cases of healthy people as healthy group.Serum HMGB1 and CA72-4 in all people were detected .Results Early gastric cancer serum HMGB1 and CA72-4 was higher than benign lesions in the stomach and the healthy group (χ2 =33.69,82.95,51.41,104.74, P<0.01),while benign lesions of the stomach and the positive rate healthy group ,the difference was not statistically significant(χ2 =3.80,1.90,all P>0.05).HMGB1 diagnosis of early gastric cancer sensitivity was 70.0%,specific-ity 95.2%and accuracy was 88.7%.CA72-4 diagnosis of early gastric cancer with a sensitivity of 80.0%(40/50), specificity was 97.2%(141/145) an accuracy of 92.8%(181/195).Joint detection,diagnosis of early gastric cancer with a sensitivity of 94.0%(47/50),while the specificity was 93.1%(135/145),the accuracy was 93.3%(182/195),higher than those of the single detection sensitivity and accuracy .Early gastric cancer with lymph node metasta-sis in patients with serum HMGB1 and CA72-4 were significantly higher than those in patients without lymph node metastasis,the two groups were significantly different (t=2.927,4.096,all P<0.05).Conclusion Joint detection of serum HMGB1 and CA72-4 allows early diagnosis of gastric cancer ,and to determine the prognosis of patients .

BACKGROUND:Bone tissue engineering is the most promising way to treat bone defects at present. The key problem is to construct vascular networks which can provide oxygen and nutrients for new bone, and thereby provide a way for the body metabolism. OBJECTIVE:To review the characteristics of bone tissue engineering scaffold materials and to introduce the development of composite scaffold materials. METHODS:With the key words of“bone tissue engineering, scaffold, vascularization, composite scaffold”in Chinese and in English, respectively, a computer-based search was performed for articles published in CNKI and PubMed databases from January 2001 to January 2014. After the initial screening, the reserved articles were further detailed, summarized and concluded. RESULTS AND CONCLUSION:According to the different sources, the bone tissue engineering scaffold materials can be divided into artificial materials, natural derivatives and composite scaffold materials. Single scaffold is difficult to be the most ideal material for repair of bone defects, while composite scaffold can make up for the defects of the single scaffold to different degrees. Therefore, in recent years, the bone tissue engineering scaffolds have developed from single to composite scaffolds and there is the trend of organic combination of artificial materials and natural derivatives. However, composite scaffolds have many problems to be solved in the clinical application. The main aspect is to control the proportion of the composite scaffold so that the degradation of materials can be matched with growth of tissues and cells. The other one is to keep the porous and high mechanical strength of the composite scaffold.

ObjectiveTo analyze the therapeutic experience and value of hemoperfusion in children with acute tetramine poisoning.MethodsAccording to the choice of children's legal guardian,67 cases with acute tetramine poisoning were divided into two groups:the perfusion group (48 cases),and the control group (19 cases).The patients in perfusion group received blood perfusion based on conventional treatment,while the patients in control group received conventional treatment only.Survival outcomes of patients with different serum tetramine concentrations in two groups were compared.ResultsWhen the serum concentration of tetramine was ＞0.5 mg/dl,patients in both groups died in a short time.When the serum concentration of tetramine was 0.20～0.49 mg/dl,the drop value of serum concentration of tetramine significantly greater in perfusion group [(0.28±0.02) mg/dl] than that in control group [(0.13±0.03) mg/dl] (P＜0.05).When the serum concentration of tetramine was ＜0.2 mg/dl,the drop value of serum concentration of tetramine in two groups showed no significant difference [ (0.12±0.02) mg/dl vs (0.11±0.03) mg/dl] (P＞0.05).Conclusion The treatment method for tetramine poisoning should be selected based on the serum concentration of tetramine.

Objective To explore the effects of lamotrigine (LTG) on cognitive function and the quality of life (QOL) in patients with partial epilepsy. Methods Twenty six patients with newly diagnosed partial epilepsy were randomly divided into 2 different groups using oral administration of LTG and carbamazepine (CBZ) respectively. By neuropsychological test scores and the Quality of Life in Epilepsy Inventory (QOLIE-31) The cognitive function and QOL were assessed before and 16 weeks after the treatment. A battery of neuropsychological tests comprised WAIS digital span test (WDST), verbal fluency test (VFT), trail making test (TMT, parts A and B), stroop color word test (SCWT), Wisconsin card sorting test (WCST), delayed logical memory test, delayed optical memory test, arithmetic ability and digital symbol conversion test. Results The repeated assessments for the patients taking LTG were associated with significant improvements in many domains. When comparing the results at the end of 16 weeks with the baseline results, the verbal fluency were improved, the time of trail making test parts A and B were shortened, the WCST correct number and classification were improved, and the persistent error number and operation time of WCST were declined as well, digital symbol conversion was increased, delayed logic memory and optical memory were improved (t=3. 043, -3. 287,-2. 543,3. 167,3. 028,-2. 191, -3. 216,3. 061,3. 036, 3. 021 ,all P<0. 01 or P<0. 05). When comparing the efficacy of LTG with the CBZ group, the time of stroop color word test was shortened, digital symbol conversion was increased and arithmetic ability was improved (t= 3. 167,2. 142,2. 101, P<0.01 or P<0. 05). Compared with the baseline, both LTG group and CBZ group showed that the overall QOL, overall health, cognitive function and social function scores were improved (LTG group: t= 3. 321,2. 462,3. 294,3. 512;CBZ group: t=3. 314, 3. 149,3. 294,3. 202,all P<0.05). When comparing LTG group with CBZ group after therapy, cognitive function and social function scores were obviously improved (t = 2. 257,2. 140, both P< 0. 05), and the worry about seizure declined (t=2. 147,P<0. 05). Conclusions LTG may improve the cognitive function and QOL in patients with newly diagnosed partial epilepsy.

The effects of cardiomyocyte grafting on left ventricular (LV) remodeling and function in rats with chronic myocardial infarction were evaluated using high-frequency ultrasound. Chronic myocardial infarction was induced in 50 Wister rats by ligating the left anterior descending artery. They were randomized into two groups: a trial group that received neonatal rat cardiomyocyte transplantation (n=25) and a control group which were given intramyocardial injection of culture medium (n=25). The left ventricular (LV) geometry and function were evaluated by high-frequency ultrasound before and 4 weeks after the cell transplantation. After the final evaluation, all rats were sacrificed for histological study. The results showed that 4 weeks after the cell transplantation, as compared with the control group, the LV end-systolic dimension, end-diastolic dimension, end-systolic volume and end-diastolic volume were significantly decreased and the LV anterior wall end-diastolic thickness, LV ejection fraction and fractional shortening were significantly increased in the trial group (P<0.01). Histological study showed that transplanted neonatal rat cardiomyocytes were found in all host hearts and identified by Brdu staining. It was suggested that transplantation of neonatal rat cardiomyocytes can reverse cardiac remodeling and improve heart function in chronic myocardial infarction rats. High-frequency ultrasound can be used as a reliable technique for the non-invasive evaluation of the effect of cardiomyocyte transplantation.
Animals, Newborn ; Echocardiography/*methods ; Myocardial Infarction/physiopathology ; Myocardial Infarction/*therapy ; Myocardial Infarction/ultrasonography ; Myocytes, Cardiac/*transplantation ; Random Allocation ; Rats, Wistar ; Ventricular Function, Left/*physiology ; Ventricular Remodeling

Objective To quantitatively describe the texture features of myocardial image in the acute myocardiac infarction (AMI) using image texture analysis. Methods Anterior descending branch of left coronary artery was ligated to set up AMI model in 11 dogs. Image texture was analyzed using grey tone co-occurrence matrix method, and 4 kinds of texture parameters including angular second moment(ASM), contrast(CON), entropy(ENT), and inverse difference moment(IDM) in normal myocardium and infarct myocardium were observed. Results A significant difference in 4 kinds of texture parameters was found between pre- and post-infarct myocardium (P

Objective To investigate the effect of deproteinised calf serum enteric-coated capsules (DCSECC) on cognitive function of patients with epilepsy.Methods Sixty-six patients were randomly divided into two groups including experimental group who received DCSECC 5 mg/d,3 times a day and antiepileptics,and control group who received antiepileptics.To detect the cognitive function by means of Wechlors memory scale (WMS),Montreal cognitive assessment (MoCA),event related potentials (P300) latency period and malondialdehyde (MDA) before treatment and after treatment for 1,2,3 months,and compared with 33 healthy subjects controls (healthy group).Results Compared with healthy group,the scores of memory quotient (MQ) and MoCA were signifiantly decreased in experimental group and control group [(80.4 ± 14.2),(79.6 ± 13.9) scores vs.(103.2 ± 30.1) scores; (13.5 ± 4.5),(12.9 ± 3.9) scores vs.(28.8 ±8.7) scores],in the mean time,P300 latency period and MDA were signifiantly prolonged [(380.8 ± 49.7),(389.5 ± 50.4) ms vs.(309.8 ± 44.7) ms ; (5.0 ± 1.8),(4.8 ± 1.7) μ mol/L vs.(2.2 ± 0.6) μ mol/L],there was significant difference (P ＜ 0.05).Compared with the control group after treatment for 3 months,the scores of MQ and MoCA in experimental group [(96.5 ± 18.3) scores,(26.8 ± 7.7) scores] were increased and P300 latency period [(321.3 ±36.1) ms] was shortened,there were significant differences (P ＜ 0.05).The level of MDA decreased significantly in experimental group after 2,3 months treatment[(3.02 ± 1.04),(2.99 ± 1.01) μ mol/L],there was significant difference(P＜ 0.05).The scores of MQ and MoCA in control group after treatment had no significant difference compared with before treatment (P ＜ 0.05).Conclusions The cognitive function in patients with epilepsy is decreased.DCSECC medical treatment can improve the cognitive function by means of antioxidant activity.

Objective To evaluate left atrial(LA)function in patients with prophase essential hypertension (EH)by left atrial volume tracking(LAVT)method.Methods Thirty prophase EH patients and 35 healthy subjects(control group)were enrolled in this study.Ultrasound LAVT(Hitachi EUB-6500)was applied to display and analyze the LA volume loop imaging on the standard LV apical two and four chamber views.The maximal LA volume at end-systole(LAVmax),LA volume at the onset of ECG-P wave(LAVP),the minimal LA volume at end-diastole(LAVmin)from the LA volume loop were recorded,and body surface area was used to revise these volume indexs(LAVImax,LAVIp,LAVImin).LA pass,act and total empting volume(LAVIpass,LAVIact,LAVItotal)and empting rate(%LAVIpass,%LAVIact,%LAVItotal),effective pass and act erupting rate(%eLAVIpass,%eLAVIaet),and the proportionality of pass empting volume and act empting volume were caculated.Results The LAVIp,LAVIact,LAVItotal,%LAVIact,%LAVItotal,%eLAVIact in the prophase EH group were significantly higher than those in the control group,wheras the LAVIpass,%LAVIpass,%eLAVIpass,LAVIpass/act were lower(all P＜0.05,or P＜0.01).Conclusions The LA volume change in prophase EH is the act empting volume increased at end-diastole in main,LAVT is a potientially useful tool to evaluate the function of LA.

The TLD(thermoluminescent dosimetry)must be calibrated before used in individual dosimetry.In this paper,some related issues that affect the accuracy of calibration,including principle and performance of the instruments,conditions of annealing and measuring,method of curve fitting are discussed and an appropriate calibration method is proposed.The results show that the method we proposed is valid and efficient,which ensures the accuracy of individual dosimetry.