Objective:To provide reference for the intensive study on polyethylene glycol modified chitosan. Methods:The relat-ed literatures at home and abroad were looked up and retrieved from 2004 to 2014, and the related contents were summarized. Results:The methods for the preparation of polyethylene glycol modified chitosan were various. Polyethylene glycol modified chitosan could be used as the carrier for nano-drug and gene therapy, the base of temperature-sensitive hydrogel, polymeric prodrug and the repair mate-rial of tissue engineering. Conclusion:Polyethylene glycol modified chitosan can be wildely used in biomedical field. However, the re-lated research is only in vitro. The in vivo studies should be further systematic and intensive.

OBJECTIVE To provide a reference for the safety of guselkumab in clinical use. METHODS The reporting odds ratio and the Bayesian confidence propagation neural network were used to mine the data of adverse drug events （ADE） related to guselkumab in FAERS database from the fourth quarter of 2017 to the second quarter of 2022. RESULTS & CONCLUSIONS A total of 29 951 ADE reports related to guselkumab were screened， involving 197 （3 871 cases） ADE signals and 21 system organs. The major ADE signals of guselkumab manifested as infectious and invasive diseases， systemic disease and various reactions at the site of administration， and skin and subcutaneous tissue　diseases， which were basically consistent with the instructions. The new ADE signals were found， such as neoplasm of orbit， gallbladder adenocarcinoma， sweat gland disorder， decreased blood uric acid， eyelid retraction， angioimmunoblastic T-cell lymphoma， nonalcoholic fatty liver disease， hyperplastic cholecystopathy， tracheomalacia， inner ear disorder， etc. And the severe ADE signals included severe infections in various parts of the body， liver and gallbladder diseases， tumor， etc.

Objective To explore the influencing factors of autoimmune thyroid disease in Xindu region and provide a basis for preventing the occurrence of autoimmune thyroiditis.Methods Using case-control study, 581 autoimmune thyroid patients treated in 2016-2020 in Chengdu Xindu District Hospital of 2020, and 450 healthy patients were selected as the control group. Relevant data were obtained and the influencing factors of adult autoimmune thyroid disease were analyzed by univariate and multivariate logistic regression model. Results Univariate results showed that the observed BMI of 30.0 kg/m², hyperlipidemia, metabolic syndrome and urinary iodine (<100 μg/L and> 200 μg/L) were higher than control group, with thyroid function [free triiodinated thyrigenine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH)), autothyroid antibody [thyroglobulin antibody (TGAb), antithyroid peroxidase antibody (TPOAb),] greater than control group (P <0.05).According to logistic regression analysis, body mass index 30.0, TGAb positive, TPOAb positive, hyperlipidemia, and metabolic syndrome were independent risk factors for adult autoimmune thyroiditis [OR (95% CI): 1.965 (1.340), 3.1262 (1.568-8.243), 3.089 (1.753-7.166), 2.507 (1.164 -4.956), 2.218 (1.207 -3.362), P <0.05]. Conclusion Body mass index of 30.0 kg/m2, hyperlipidemia and metabolic syndrome are the risk factors for adult autoimmune thyroiditis in Xindu region.Controlling weight, controlling autoimmune thyroiditis and metabolic syndrome are the key to reducing adult autoimmune thyroiditis in Xindu region.

OBJECTIVE: To analysis clinical phenotype and potential genetic cause of a family affected with hereditary coagulation factor Ⅻ deficiency. METHODS: The prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D-Dimer (D-D), coagulation factor Ⅻ activity (FⅫ:C) and coagulation factor Ⅻ antigen (FⅫ:Ag) were determined for phenotype diagnosis of the proband and his family members(3 generations and 5 people). Targeted capture and whole exome sequencing were performed in peripheral blood sample of the proband. Possible disease-causing mutations of F12 gene were obtained and further confirmed by Sanger sequencing. The corresponding mutation sites of the family members were analyzed afterwards. The online bioinformatics software AutoPVS1 and Mutation Taster was used to predict the effects of mutation sites on protein function. RESULTS: The APTT of the proband was significantly prolonged, reaching 180.9s. FⅫ:C and FⅫ:Ag of the proband was significantly reduced to 0.8% and 4.17%, respectively. The results of whole exome sequencing displayed that there were compound heterozygous mutations in F12 gene of the proband, including the c.1261G＞T heterozygous nonsense mutation in exon 11 (causing p.Glu421*) and the c.251dupG heterozygous frameshift mutation in exon 4 (causing p.Trp85Metfs*53). Both mutations are loss of function mutations with very strong pathogenicity, leading to premature termination of the protein. AutoPVS1 and Mutation Taster software predicted both mutations as pathogenic mutations. The results of Sanger sequencing revealed that c.1261G＞T heterozygous mutation of the proband was inherited from his mother, for which his brother and his daughter were c.1261G＞T heterozygous carriers. Genotype-phenotype cosegregation was observed in this family. CONCLUSION The c.1261G＞T heterozygous nonsense mutation in exon 11 and the c.251dupG heterozygous frameshift mutation in exon 4 of the F12 gene probably account for coagulation factor Ⅻ deficiency in this family. This study reports two novel pathogenic F12 mutations for the first time worldwide.
Blood Coagulation Disorders ; Codon, Nonsense ; Factor XII/genetics* ; Female ; Heterozygote ; Humans ; Male ; Mutation ; Pedigree

Objective:To observe the effect of Saposhnikoviae Radix-Mume Fructus containing-serum in regulating the phenotypic transformation of airway smooth muscle cells (ASMCs) proliferation model, in order to explore the mechanism of combined administration of "Saposhnikoviae Radix, Mume Fructus" in inhibiting airway remodeling, and reveal the compatibility mechanism of traditional Chinese medicine. Method:The proliferation model of ASMCs was established by platelet derived growth factor (PDGF) induction. The rats were given normal saline, Saposhnikoviae Radix-Mume Fructus, Saposhnikoviae Radix-Mume Fructus(15.425, 15.425, 30.85 g·kg^-1·d^-1) to prepare drug serum respectively. Four generations of logarithmic phase human bronchial smooth muscle cells (HBSMC) were collected and divided into blank control group, cell model group, normal rat serum group and normal rat serum cell model group, hormone intervention group, Saposhnikoviae Radix serum group, Mume Fructus serum group, Saposhnikoviae Radix-Mume Fructus serum group. The cells were given corresponding treatment. Immunofluorescence staining and Western blot were adopted to detect ASMCs deflating marks protein α-actin and osteopontin (OPN) expressions, and phenotypic transformation was observed; the levels of vascular endothelial growth factor(VEGF), transforming growth factor-β(TGF-β) and interleukin-6(IL-6) secreted by ASMCs were detected by enzyme linked immunosorbent assay (ELISA). Result:Compared with blank group and normal rat serum group, the fluorescence intensity and protein expression of α-actin in model group and normal rat serum cell model group were low, whereas the fluorescence intensity and protein expression of OPN were high, and the concentrations of VEGF, TGF-β and IL-6 increased significantly (P<0.05). Compared with model group and normal rat serum cell model group, Saposhnikoviae Radix-Mume Fructus serum group and hormone intervention group could significantly enhance the fluorescence intensity and protein expression of alpha-actin, decrease the fluorescence intensity, protein expression of OPN and concentrations of VEGF, TGF-β and IL-6 (P<0.05). Conclusion:The combined administration of "Saposhnikoviae Radix-Mume Fructus" has an inhibitory effect on airway remodeling, which may be related to the inhibition of the transformation of ASMCs from contractile phenotype to synthetic phenotype, so as to reduce the release of active substances, such as VEGF, TGF-β and IL-6.

ObjectiveTo investigate effect of conducting training of autism spectrum disorder （ASD） early screening skill on improving the ability to early identify ASD of medical staffs in primary care hospitals. MethodsIn September 2021， the training of ASD early screening skills was carried out for medical staffs from 20 primary care hospitals in Chengdu. After training， the training effect was evaluated. The numbers of referrals from primary care hospitals to superior hospitals， confirmed ASD as well as their average diagnostic age of children with ASD before and after training were used as evaluation indicators. ResultsAfter training， the number of children with suspected ASD referred by primary care hospitals was more than that before training ［（16.65±11.60） vs. （3.40±2.23）， t=5.431， P<0.01］， the number of children diagnosed with ASD was more than that before training［（6.85±4.93） vs. （2.45±1.67）， t=4.171， P<0.01］， and the differences were statistically significant. As for the diagnosed age of ASD children， after training， the average age was lower than that before training ［（34.95±11.67） vs. （42.2±14.64）， t=-2.553， P=0.019］. ConclusionTraining of ASD early screening skills for medical staffs in primary care hospitals may help to improve their ability to early screening ASD children.