OBJECTIVE To understand the knowledge about nosocomial infection of the personal. METHODS A questionnaire investigation was carried out by the hospital local area network(LAN). RESULTS A total of 202 questionnaires were sent in during one month, and statistic result could be looked up at any moment. CONCLUSIONS Questionnaire investigation via LAN can find the shortcomings of the nosocomial infection management correctly and quickly.

Objective To compare the characteristics of remifentanil and sufentanil for tracheal intubation general anesthesia in gyneco-logical laparoscopic operation. Methods 80 patients received gynecological laparoscopic operation were randomly divided into the remifen-tanil group and the sufentanil group with 40 patients in each group, and they were performed total intravenous anesthesia with remifentanil or sufentanil respectively. Compare the changes of heart rate, mean arterial pressure in the two groups before and after induction, at the time of intubation, pneumoperitoneum, and after the operation. And differences in two groups were recorded in the recovery time, extubation time, postoperative pain in 24 hours, exhaust time and postoperative complications. Results Change trend and magnitude in heart rate, mean ar-terial pressure had no significant difference in the two groups (P>0. 05). The recovery time, extubation time in remifentanil group was shor-ter (P<0. 01), postoperative pain in 24 hours in sufentanil group was more mild (P<0. 01). Exhaust time and postoperative complications of the two groups had no significant difference (P>0. 05). Conclusion Remifentanil and sufentanil used in gynecological laparoscopic op-eration can achieve good effect. and effects of the two drugs on hemodynamics were similar. Remifentanil can recieve better effect in terms of recovery time, while sufentanil has better postoperative analgesia effect.

Objective:To investigate the optimal water extraction process of Jinyin Lidan oral liquids. Methods:An orthogonal de-sign was used in the extraction optimization using the yield and content of chlorogenic acid,narigin and neohesperidin as the evaluation indices and the volume of water,extraction times,extraction time and soaking time as the influencing factors. The contents of chloro-genic acid,narigin and neohesperidin were detected by HPLC to screen the best extraction technology. Results:The best water extrac-tion conditions were as follows:the amount of the added water was 8 times of the medicinal materials,the soaking time was 1 h,and the decoction was carried out twice with the duration of 1. 5 h for each. Conclusion:The process is stable and reasonable,which pro-vides research foundation for the production and quality control.

Objective To establish the quality standard of Jiangzhi Fugan Capules. Methods TLC was used to the qualitative identification of Paeoniae Radix Rubra and Salviae Miltiorrhizae Radix et Rhizoma. The contents of Paeoniflorin and Tanshinone ⅡA were determined by HPLC. The HPLC separation was performed on Phenomenex C18 column (4.6 mm×250 mm, 5 μm), with methanol-0.1% phsophonic acid (31∶69, 75∶25) as mobile phase, and the flow rate was 1.0 mL/min. Results The results of TLC showed that relevant spots were clear without interference against the negative sample. The calibration curves for Paeoniflorin and Tanshinone ⅡA were found to be liner within the range of 0.448-4.48μg, 0.057 6-0.576μg, respectively. The correlation coefficients were 0.999 4 and 0.999 5, respectively. The average recoveries were 99.93%and 99.75%, with RSD of 1.98%(n=9) and 1.70%(n=9), respectively. Conclusion The method is accurate, reliable, stable, rapid, and reproducible, and can be used for the quality control and evaluation of Jiangzhi Fugan Capules.

Objective To investigate the effect of salinity and temperature on motility of Vibrio parahaemolyticus. Methods V.parahaemolyticus was inoculated on swarming or swimming agar plates containing different amounts of salinity (0.5%, 1.0%, 2.0%, and 4.0% NaCl, respectively), followed by incubation at 26 or 37℃, before the diameters of bacterial lawns were measured .Results and Conclusion The swarming motility was not affected by salinity , while the swimming motility was positively correlated with salinity .Maximum swimming occurred in 2.0% NaCl, and displayed a slight decline in salinity of 4.0%.Both swimming and swarming were affected by temperature , and the motility was signifi-cantly enhanced in 37℃vs 26℃.These results indicate that both salinity and temperature can modulate the motility of V. parahaemolyticus.

Objective To establish the quality standard for Jinyin Lidan Oral Liquid. Methods Artemisiae Scopariae Herba, Taraxaci Herba, Aurantii Fructus and Schisandrae Chinensis Fructus in Jinyin Lidan Oral Liquid were identified by TLC. The contents of naringin and neohesperidin were determined by HPLC. The HPLC separation was performed on Phenomenex C18 column (4.6 mm × 250 mm, 5 μm) with the mobile phase of methanol-0.1% phsophonic acid solution and gradient elution. The UV detection wavelength was 254 nm;flow rate was 1.0 mL/min;column temperature was 25 ℃. Results The results of TLC showed that relevant spots were clear, with strong specificity, without interference of negative sample. The calibration curves for naringin and neohesperidin were in good linearity in the range of 0.56-5.60μg (r=0.999 9) and 0.38-3.80μg (r=0.999 8), respectively. The average recoveries were 99.86%and 98.95%with RSD of 2.04%and 2.45%, respectively. Conclusion The qualitative and quantitative determination method is simple, accurate and reliable, with good stability and reproducibility, and can be used for the quality control of Jinyin Lidan Oral Liquid.

In the last few years, high-throughput (or 'next-generation') sequencing technologies have delivered a step change in our ability to sequence genomes, whether human or bacterial. Further comparative genome analysis enables us to reveal detailed knowledge of genetics or physiology of industrial important strains obtained in laboratory, to analyze genotype-phenotype correlations of mutants with improved performance. Based on identified key mutations or mutation combinations, Inverse Metabolic Engineering (IME) can be performed by using accurate genetic modification system. Recently, IME has been successfully used for strain improvement and has become a research hotspot, including improving substrate utilization, engineering the robustness of industrial microbes and enhancing production of bio-based products. Here, we describe recent advances in research methods of IME, with an emphasis on characterization of genotype-phenotype and the latest advances and application of IME. Possible directions and challenges for further development of IME are also discussed.
Industrial Microbiology ; trends ; Metabolic Engineering ; trends ; Mutation

Objective To investigate the effect of oncolytic adenovirus vector SG600-IL24expressing human melanoma differentiation associated gene-7 (mda-7/IL-24) on hepatocellular carcinoma cell lines with different metastatic potential of HepG2, SMMC7721, MHCC97L and normal liver cell line LO2. Methods The oncolytic adenovirus SG600-IL24 which carrying mda-7/IL-24 gene was transfected into hepatocellular carcinoma cell lines and normal liver cell line. The mRNA and protein expression of mda7/IL-24 in HepG2, SMMC7721, MHCC97L and LO2 cell lines was confirmed by RT-PCR,ELISA assay and Western blot respectively. MTT assay and flow cytometry were used to study tumor cell proliferation and cell cycle in vitro. Hoechst33258 and flow cytometry were studied to indicate the apoptosis effects. Results It was confirmed by RT-PCR, ELISA assay and Western-blot that the exogenous mda-7/IL-24 gene was highly expressed in HepG2, SMMC7721, MHCC97L and LO2 cell lines. MTT and apoptosis detection indicated that MDA-7/IL-24 can induce the growth suppression (the inhibition rate was 75% ±2. 5% ,86% ±3. 5% ,and promotes apoptosis ( the apoptosis rate was 56. 5% ± 4. 0% , 34. 4% ± 2. 0% , 43. 3% ± 2. 5%cell lines at G2/M phase ( the blocking rate was 35. 4% ± 4. 2% , 40. 5% ± 5. 0% , 42. 0% ± 5. 0%metastatic potential hepatocellular carcinoma cell lines but not in normal liver cell line.Conclusions Oncolytic adenovirus vector SG600-IL24 can selectively induce growth suppression, promote apoptosis in hepatocellular carcinoma lines in vitro but not in normal liver cell LO2.