OBJECTIVE:To investigate the epidemiological characteristics of adverse reaction(ADR) caused by Shenmai injection.METHODS:By retrieving from Chinese medical periodicals issued in 1990 to 2010,145 cases of ADR induced by Shenmai injection were collected to establish database using ACCESS.Based on established database,the epidemiological information was analyzed statistically.RESULTS:The incidence of ADR was the highest in the middle-aged and old sufferers(87.6%),and was 37.9% in the male(n=55) and 62.1% in the female(n=90).Of primary diseases cases,cardiovascular disease took up 62.1%.The incidence of allergic reaction took up 38.0% in ADR cases and ADRs usually occurred within 30 min after first injection(64.8%).CONCLUSION:The establishment of ADR database is beneficial to the storage and analysis of ADR information and provided reference for the prevention and therapy of ADR caused by Shenmai injection.

OBJECTIVE: To establish the quality standard of l of Zengshi oral liquid. METHODS: Thin-layer chromatography(TLC) was employed for the qualitative determination of the Lycium barbarum,Cassia obtusifolia and schisandra chinensis. High performance liquid chromatography (HPLC) was applied for the determination of chrysophanol in Zengshi Oral Liquid. RESULTS: The quality standard of Zengshi oral liquid could be established through qualitative determination of the Lycium barbarum,Cassia obtusifolia and Schisandra chinensis.The linear range of Chrysophanol was 0.083～0.416?g (r=0.999 9). The average recovery was 98.38% and the RSD was 1.72%. CONCLUSION: The established standard can be used for the quality control of Zengshi oral liquid.

AIM: To establish the method of determining five anthraquinone components in Shaotangshang Gel(Rhizoma Coptidis,Cortex Phellodendri Chinensis,Radix et Rhizoma Rhei,etc.). METHODS:HPLC was used to determine emodin,chrysophanol,rhein,physcion,alone-emodin in Shaotangshang Gel.The separation was performed on Yilited-C_(18) Column with MeOH-phosphate acid(0.1%)(80∶20) as the modile phase,the flow rate was 1.0 mL/min and detection wavelength was set at 430 nm. RESULTS: The resolution and the linearity of the method were good with the average recoveries and RSD values of emoldin,chrysophanol,rhein,physcion,aloe-(emodin) of(98.0%),1.3%;97.1%,1.8%;97.8%,1.5%;97.4%,1.7%;96.7%,1.2% respectively.CONCLUSION: This method is sensitive,accurate and can be used for the quality control of Shaotangshang Gel.

Objective To explore the maternal toxicity and offspring's neurobehavioral effects in Wistar rats induced by exposure to methylmercury during pregnancy. Methods Methylmercury were continuously orally perfued with different doses of 0.00, 0.01, 0.05 and 2.00 mg/(kg?d) to 52 pregnant Wistar rats during the 6th～9th day after conception. The toxicity to maternal rats was observed and general teratology test were carried out. The parameters of physical and neurobehavioral develpment at early stage of 205 offspring were measured and the operant behavior test of 32 offspring was carried out at the age of 10 weeks. The brain's morphology and the levels of monamine neurotransmitter were tested in 24 maternal rats after 5 weeks of the delivery and 24 offspring in 10 weeks old. Double blind randomized design was used in whole experiment. Results No maternal toxicity and offspring teratogenicity were observed. The increase of body weight and tail development of embryos in 3 exposure groups were slower than those of control group (P

0.05).But the female offspring in exposure group showed weaker capability of spatial location compared with that of male offspring in exposure group(P

OBJECTIVE:To establish the quality criteria of Kesuting oral liquid. METHODS: Qualitative identification of Herba Ephedrae,Folium Mori and Radix Et Rhizoma Glycyrrhizae was conducted using TLC method. The content of Baicalin was determined by HPLC in which the chromatographic separation was performed on Spherigel ODS C18(250mm?4.6mm,5?m) column with mobile phase consisted of methanol-0.4%phosphate solvent(52∶48) at a flow rate of 1.0 mL?min-1. The UV wavelength was set at 280 nm. RESULTS: The TLC spots of Herba Ephedrae,Folium Mori and Radix Et Rhizoma Glycyrrhizae were characteristic and obvious, and which can be used for qualitative identification. The linear range of Baicalin was 0.24～1.20 ?g (r=0.999 8) with a mean recovery rate of 98.72%(RSD=1.02%,n=6). CONCLUSION:The establish criteria are suitable for the quality control of Kesuting oral liquid.

OBJECTIVE:To study the correlation between the rate of inpatients using antibacterials and the cross-sectional rate of inpatients using antibacterials.METHODS:A cross sectional study was conducted on the use of antibacterials in 1040 inpatients between May 22 and May 23 in 2007.Besides,the discharge case histories of 100 cases were collected randomly for an analysis.RESULTS:Of the total 1 040 inpatients,510(49.04%)used antimicrobials.The rate of inpatients using antibacterials in comprehensive ICU took the lead at 100%,followed by pediatric department(86.05%)and the department of nephrology(84.62 %).The average rate of inpatients using antibacterials in surgery department was significantly higher than in the department of internal medicine(63.52% vs.33.94%).81% of the discharged cases used antibacterials.The irrational use of antibacterials manifested as improper timing of medication,prolonged use of antibacterials for prophylactic purpose etc.CONCLUSION:The proportion of the patients using antibacterials in descending order were inpatients,cross-sectional inpatients and outpatients.It is necessary to tighten control on the rational use of antimicrobials.

OBJECTIVE:To provide a method for a scientific and rational formation of hospital drug purchase plan.METHODS:Based on the major factors influencing the drug purchase plans,a moving-average method(mathematical principle)was used to establish computational formula for hospital drug purchase plan model,which was then put into practice.RESULTS:The predicted amount calculated using the computation model could be used to track the actual consumption quantity rapidly;this model could auto-adjust the inventory and dynamically forecast the drug purchase amount.CONCLUSION:The computation model contributes to the scientific and reasonable formation of hospital drug purchase plan thus meeting the needs of majority clinical drugs.

Objective To investigate the genetic effect of low power microwave radiation on the anomaly of sperm and micronucleus(MN) formation in the marrow polychromatic erythrocyte (PCE) in rat. Methods Rats were randomly divided into three groups, namely control, experimental group Ⅰ and experimental group Ⅱ. Abnormal sperms and MN formation in marrow PCE were observed and detected after exposure to 1 800 MHz (Continuous wave, CW) microwave radiation for 21 days (12 h/d) with the average power density of 0 mW/cm 2 (control), 0 5 mW/cm 2 (group Ⅰ) and 1 0 mW/cm 2 (group Ⅱ). Results No significant difference in the rate of sperm abnormality and MN formation in marrow PCE was found between the experimental groups and the control. Conclusion No obvious genetic damage caused by low power microwave radiation to the sperm anomaly and MN formation in marrow PCE of rats is found.

Objective To constructan eukaryotic expression recombinant plasmid named pEGFP-N2-PRNP .Methods Total RNA was extracted from alzheimer (AD) disease peripheral blood ,and the PRNP gene was amplified by reverse transcription-poly-merase chain reaction(RT-PCR) .By using gene recombination technique ,human PRNP cDNA was inserted into retroviral vector pEGFP-N2 .The recombinant plasmid was identified by a pair of specified primers containing the restriction sites of Xho Ⅰ and EcoRⅠ .Results The PRNP gene could be obtained by RT-PCR ,the recombinant plasmid was identified by restriction endonucle-ase analysis ,PCR and sequence analysis ,and the expression vector pEGFP-N2-PRNP ,which could be stably expressed in SH-SY5Y cells .Conclusion The recombinant plasmid pEGFP-N2-PRNP is constructed successfully ,Which offers a basic for the further re-search on PRNP biological fuction .