The tissue engineering heart valve(TEHV) is a kind of procthetic valve that possesses the characteristics of living, self-repair and proliferation. Owing to the specific properties that are the better hemodyna-mics, low or even no immunological reaction, no need for long-time anticoagulation therapy and longer durability, the ideal TEHV can overcome the drawback of current available valve substitutes which are mechanical prostheses and biological heart valves. In this paper we reviewed the recent development of the TEHV in such aspects, as biomaterial, cultivating environment, seed cells and so on. In addition, we briefly introduced the perspective of using human embryonic stem cells as the source for seed cells in TEHV.

Objective To recombine the tissue plasminogen activator (TPA) gene and establish the expressing model in vitro to provide both theoretical basic and technical guidance for gene therapy of ischemic heart disease and prevention of postoperative vessel re-stenosis. Methods Expression vector pcDNA3.1 TPA was constructed, and transfected into Chinese hamster ovary(CHO) cells. Then the exogenous TPA expression was observed. Results The expression quantity of TPA in transfected CHO cells was higher than that in non-transfected cells. The exogenous TPA activity was 12 296 IU/10 6cell/24hr in the transfected cells, when measured by chromogenic substrate assay, while that of non-transfected cells was 3 176IU/10 6cell/24hr. The TPA quantity of non-transfected cells was 9 608 ng/10 6cell/24hr, when measured by enzyme-linked immunosorbent assay (ELISA), while that of transfected cells was 586 172 ng/10 6 cell/24hr, and the latter was the 60 times of the former. Conclusions When pcDNA3 1(+)TPA was transfected into CHO cells, exogenous TPA can efficiently be expressed, which provides theoretical basic for TPA clinical gene therapy.

Objective To explore the special technique and the brain protection method of cardiopulmonary bypass(CPB) in large artery operation involving in aortic arch. Methods The procedures of cardiopulmonary bypass and the clinical outcome of 52 patients, who received large artery operation involving in aortic arch, were analysed retrospectively. Results The conventional hypothermal cardiopulmonary bypass was performed as basic method in all patients, deep hypothermal circulatory arrest were used in 13 patients, deep hypothermal circulatory arrest plus retrograde cerebral perfusion in 12 cases, selective antegrade cerebral perfusion in 7 cases, and separate perfusion of upper and low body in 20 cases. The total mortality was 17 3%(9/52), and there were 3 patients with the various severitits of cerebral complications. The mean cardiopulmonary bypass time was (180 6?51 8)min and the mean aortic block time was(62 4?61 9)min. Conclusion During the large artery operations involving in aortic arch, in order to prolong the period of deep hypothermal circulatory arrest, improve the effects of brain and spinal protection and minimize operative complications, different perfusion techniques should be employed according to different artery lesions and operative approaches.

Objective To explore the application of left posterior lateral thoracotomy in the reoperation of mitral vavle. Methods Between April 1995 and April 2003, 11 cases of postoperative recurred mitral valve diseases received reoperation through a left posterior lateral thoracotomy under cardiopulmonary bypass via descending aorta for artery line and main pulmonary artery cannulation for venous line. Results There were no operative and post-operative deaths. The mean CPB time was 138 min. Blood loss of postoperation was less than 300ml in each patient. Conclusion Left posterior lateral thoracotomy in the reoperation of postoperative recurred mitral valve diseases had a minimal adhesiotomy. Extracorporeal circulation via descending aorta and main pulmonary artery cannulation proved easy to do and reliable. This operative approach was simple and safe.

Objective To sum up the diagnosis and treatment of airway complication after heart-lung transplantation. Methods One patient who occurred dyspnea, cough and wheeze at the 200th day after heart-lung transplantation, was diagnosed suffering from anastomosis stenosis. Comprehensive multimodality approach such as rigid bronchoscopic debridement and laser photoresection were performed. Eventually, this case was surgically managed with sleeve resection of stenotic bronchial segment. Results Surgical operation by sleeve resection of stenotic segment was successful, and the patient's symptoms disappeared. Till now the patient has been living more than 14 months after transplant, and CT scanning and fibrous bronchoscopy examination showed that the anastomasis healed well. Conclusion Airway complication's early recognition and proper management could improve the patients symptoms and survival rate.

Objective To analyze the characteristics of cardiovascular two-dimensional (2-D) and Color Doppler echocardiography for patients with Marfan's syndrome. Method 23 cases of Marfan's syndrome were examined by transthoracic 2-D and Color Doppler echocardiography.Results Aneurysms of the ascending aorta and aortic sinusal were found in all of these cases. Aortic regurgitation and left ventricular enlargement coexisted with all of these cases. Fourteen cases complicated with type A and three with type B aortic dissection. Conclusions 2-D and Color Doppler echocardiography is a rapid non-invasive,reproducible, comprehensive medical imaging technique and can be regarded as an initial imaging model to screen and diagnosis of Marfan's syndrome.

Objective To evaluate the recent curative efficacy and security of High Intersity Focused Ultrasound(HIFU)in treating patients with advanced pancretic carcinoma.Methods 17patients with cancer of pancreas were treated by FEP-BY02,received 135(average 8)times.Results The overall response rate was 70%(CR+PR),including11.76%(2/17)complete response(CR)and 58.82%(10/17)partial response(PR).The clinical benefit response(CBR)was 82.36%.The inefficiency was 17.64%(3/17).The cancer pain was relieved in 80% patients(8/10).Nocomplications occurred.such as pancreatic juice leakage,bleeding,perforation of gastrointestinal tracts,scalding of skin in the group.Conclusions The preliminary results show that HIFU has a high efficacy and quite safety for advanced pancreatic carcinoma,it is worthy of futher clinical trail.

The designed experiment of nutrition survey is set up in the medical teaching in order to make the medical students realize the seriousness and the creativity of the scientific research and to improve their interest and ability of the scientific research.

Objective To compare the differences in behavior characteristics among SHR, WKY and SD rat models of attention deficit/hyperactivity disorder (ADHD), and explore an ideal control model of SHR rats.Methods Using open field test to analyze the rat movement distance, speed, wearing numbers and the number of grooming to evaluate the spontaneous movement in SHR, WKY and SD rats.Using the Morris water maze to test the learning and memory ability among the three rat groups.Results The result of open field test showed that the SHR rats had significantly increased (P< 0.01) total amount of exercise, average speed and wearing numbers than WKY and SD rats.Compared with the WKY rats, SD rats had a significantly higher movement distance (P< 0.01), slightly higher movement speed and wearing number (P< 0.05).In the Morris water maze hidden platform period test, the SD rats had a significantly longer latency than the SHR rats (P< 0.05).SD rats showed longer latency distance on the first, third and fourth days of training, as compared with the SHR rats (P< 0.05 or P< 0.01).Compared with the WKY group, SD rats showed a shorter latency distance in each training time (P< 0.05 or P< 0.01).In the probe trial period, the SD rats showed shorter time and distance ratio to the target quadrant than SHR rats (P< 0.05), while significantly longer than the WKY rats (P< 0.05 or P< 0.01).Conclusions There are significant behavioral differences between SHR and WKY rats, showing certain disadvantages in comparison of the two types of rats.To add SD rats as a control group for SHR rats can improve the comparability of behavior characteristics of SHR rats, and to get more objective evaluation of the behavior characteristics of SHR rats.

Objective To discuss the causes of misdiagnosis and therapeutic errors of tracheobronchial foreign bodies.Methods 139 cases of tracheobronchial foreign bodies hospitalized in our department during 5 years were reviewed. Retrospective analysis was performed, on statistics of case history, clinical manifestation, location of foreign bodies, the time of initial diagnosis and the time for definite diagnosis.Results 89 cases (64 03%) were misdiagnosed, and were given inappropriate treatments. The main causes include ignored or vague history of foreign body inhalation, the atypical or complex clinical manifestations, and underestimation of the importance of bronchoscopy.Conclusion We should pay more attention to history of foreign body ,bronchoscopy examination action in diagnosis and treatment and more aware of the various manifestations of tracheobronchial foreign bodies,these could contribute to decrease the misdiagnosis and therapeutic errors.