Objective:To explore the influencing factors of BCP region A1762T/G1764A and Pre-C region G1896A mutations in patients with chronic hepatitis B virus（HBV）infection.Methods:Clinical data of 464 patients with chronic HBV infection admitted to Ningbo No.2 Hospital from October 2010 to August 2022 was retrospectively analyzed. The mutations in the BCP region A1762T/G1764A and the pre-C region G1896A were examined in all patients. Logistic regression was used to analyze the influencing factors of these mutations.Results:The mutation rate of A1762T/G1764A and G1896A was 62.1%（288/464）and 32.8%（152/464）；and the co-mutation rate of A1762T/G1764A and G1896A was 20.9%（97/464）. The mutation rate of A1762T/G1764A and the co-mutation rate of A1762T/G1764A and G1896A in patients with end-stage liver disease were higher than those in patients with chronic hepatitis B（ χ2=9.285 and 6.748，both P<0.05）. Univariate analysis showed that A1762T/G1764A mutation was associated with higher age，serum AST levels，and proportion of genotype C（ P<0.05 or <0.01）；while G1896A mutation was associated with higher age，proportion of negative HBeAg，and serum GGT levels（ P<0.05 or <0.01）. Multivariate logistic regression analysis indicated that higher age was an independent influencing factor for A1762T/G1764A mutation（ OR=1.035，95% CI 1.017-1.054， P<0.001），while HBeAg status was an independent influencing factor for G1896A mutation（ OR=0.171，95% CI 0.112-0.261， P<0.001）. Conclusion:Chronic HBV infection patients with higher age is likely to have A1762T/G1764A mutation，while those with negative HBeAg is more likely to have G1896A mutation.