1.The impact of non-alcoholic fatty liver disease on islet function in type 2 diabetes
Lihui YAN ; Shaozhen WANG ; Yakun FU ; Xinli WANG ; Xin ZHENG ; Yadan ZHENG ; Xiuli LIU
Chinese Journal of Internal Medicine 2015;54(3):197-200
Objective To analyze the influence of hepatosteatosis on pancreatic β-cell function in type 2 diabetes mellitus (T2DM).Methods A total of 213 subjects with T2DM from Metabolic Disease Hospital,Tianjin Medical University from January 2013 to December 2013 were included in the study.Non-alcoholic fatty liver disease (NAFLD) was diagnosed with abdominal ultrasonography.Patients were divided into two groups:T2DM with NAFLD and T2DM without NAFLD.ALT,AST,γ-glutamyltransferase,serum lipid,glycosylated hemoglobin A1 c (HbA1c),fructosamine,fasting glucose,insulin and 2 hours plasma glucose,insulin after 75g glucoseload were detected.The insulin resistance and β-cell function were assessed by HOMA-IR and HOMA-β.Results Among the 213 T2DM subjects,51% (108 cases) were with NAFLD.The HOMA-IR [4.76 (2.83,7.21) vs 2.79 (1.76,4.37),P < 0.05] and HOMA-β [49.18 (37.78,85.09) vs 29.50 (18.09,45.54),P < 0.05] were significantly higher in T2DM with NAFLD than those in T2DM alone.Within subjects with T2DM and NAFLD,the HOMA-IR [6.28 (2.87,8.17) vs 2.95 (2.07,3.66) P < 0.05] and HOMA-β [59.18 (37.78,85.09) vs 30.59 (28.56,34.49),P < 0.05] levels were higher in subjects with normal liver function than those with abnormal liver function.Conclusions T2DM patients with NAFLD have severer insulin resistance than those without NAFLD.The β-cell function of those patients was compensatory increased,which was decreased in subjects with abnormal liver function.
2.Molecular basis of UVA-induced skin aging and arotinoid ethyl ester protection.
Zhung-yong LIU ; Guo-wei ZHANG ; Guo-fu YAN ; yong-xin LEU ; Yun-zhi HE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(5):384-385
Benzoates
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pharmacology
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Cells, Cultured
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Cellular Senescence
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drug effects
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radiation effects
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Gene Expression Regulation, Enzymologic
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drug effects
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radiation effects
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Humans
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Infant, Newborn
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Male
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Matrix Metalloproteinase 1
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genetics
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Matrix Metalloproteinase 3
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genetics
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Methoxsalen
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pharmacology
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RNA, Messenger
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genetics
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metabolism
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Retinoids
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pharmacology
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Skin
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cytology
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metabolism
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Time Factors
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Tissue Inhibitor of Metalloproteinases
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metabolism
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Ultraviolet Rays
3.Normal reference value of forced vital capacity of Chinese younger women and geographical factors
Miao GE ; Yaping ZHANG ; Jinwei HE ; Yanchun YAN ; Xin WANG ; Lixia CAO ; Haiyan FU
Journal of Pharmaceutical Analysis 2010;22(1):34-38
Objective To supply a scientific basis for standardizing the normal reference value of forced vital capacity (FVC) of Chinese younger women. Methods We studied the relationship between the normal reference value of 21767 samples of FVC of younger women and eight geographical factors in 157 areas in China. Results It was found that the correlation between geographical factors and the normal reference value of FVC of younger women was quite significant (F=5.884, P=0.000). By adopting the method of mathematical regression analysis, one regression equation was inferred: =3.146+0.00006919X1+0.01315X4-0.006966X6+0.09524X8±0.254. In the above equation, is the normal reference value of younger women' FVC (L); X1 is the altitude (m); X4 is the annual mean air temperature (℃); X6 is the annual mean relative humidity (%); X8 is the annual mean wind speed (m/s); 0.254 is the value of the residual standard deviation. Conclusion If geographical values are obtained in a certain area, the normal reference value of FVC of younger women in this area can be obtained by using the regression equation. Furthermore, according to the geographical factors, China can be divided into eight regions: Northeast China Region, North China Region, Shanxi-Shaanxi-Inner Mongolia Region, middle and lower reaches of the Yangzte River Region, Southeast China Region, Northwest China Region, Southwest China Region, and Qinghai-Tibet Plateau Region.
4.Mechanical property of different cross-sectional area screws in middle and upper thoracic vertebral pedicle-rib complex
Daqi XIN ; Hongjun HUO ; Xuejun YANG ; Wenhua XING ; Yan ZHAO ; Yu FU ; Yong ZHU ; Feng LI
Chinese Journal of Tissue Engineering Research 2014;(9):1356-1361
BACKGROUND:Due to the importance of pedicle adjacent structures, once the screw replacement appears a deviation, adjacent structures may be damaged, leading to extremely serious consequences. Although the security of screw placement in thoracic vertebral pedicle-rib complex is significantly greater than that of pedicle screws, the mechanics of the pedicle-rib complex at different cross-sectional areas of the screw are rarely reported.
OBJECTIVE:To observe mechanical property of different cross-sectional area screws in the middle and upper thoracic vertebral pedicle-rib complex.
METHODS:Five specimens of adult cadaveric thoracic spine (T 1-T 10 ) and adjacent rib segment (50-60 mm long) were used. The bone density of specimens was measured using difunctional bone density testing machine, and osteoporotic vertebral body was excluded. The position of the screws was detected with CT images. The maximal withdrawal force of the pedicle screw was measured with biomechanical force test machine.
RESULTS AND CONCLUSION:Thirty-eight specimens at normal bone density were implanted with 25 screws (5.5 mm), 25 screws (6.0 mm) and 26 screws (6.5 mm). Because the pedicle screws destroyed the pedicle-rib complex and perforated the vertebral body, we final y obtained the withdrawal force of 68 screws. The axial withdrawal force of pedicle screws at different diameters was (812.36±147.22) N, (868.64±160.48) N and (946.48±157.58) N, respectively. There were significant differences between the 5.5 mm screws and the 6.5 mm screws (P<0.05). Experimental findings indicate that, the pedicle screws (diameter>5.5 mm) are suitable in the middle and upper thoracic vertebral pedicle-rib complex due to strong internal fixation and clinical requirement.
5.EXPRESSION OF ANNEXINA5 IN HUMAN UTERINE CERVICAL SQUAMOUS CELL CARCINOMAS
Xin LI ; Fulu GAO ; Jiantuan LI ; Yong YAN ; Guangli FU ; Qian XU
Acta Anatomica Sinica 2002;0(06):-
Objective To observe whether the expression of Annexin A5(ANXA5) changes in human uterine cervical squamous cell carcinomas(UCSCC).Methods 25 UCSCC tissues and 15 normal human uterine cervical tissues were collected.Each sample was lysed in lysis buffer.Whole protein of the supernatant was estimated by BCA-100 Protein Quantitative Analysis Kit.The expressions of ANXA5 in UCSCCs and normal uterine cervical tissues were detected respectively with Western blotting.To further ensure the expression of ANXA5 in UCSCCs,another 45 UCSCC specimens and 20 normal cervical tissues were collected.ANXA5 expression was detected by in situ hybridization and immunohistochemistry respectively.Results The staining of ANXA5 band with Western blotting in UCSCCs was much heavier than that in normal uterine cervical tissues and the expression of ANXA5 was found much higher in UCSCCs by immunohistochemistry and in situ hybridization.Conclusion Expression of ANXA5 was up-regulated in human UCSCCs.There's some relationship between uterine cervical squamous cell carcinoma and ANXA5 protein.
6.ILC2 regulates chronic renal failure patients' immunity by secretion of IL-13 through activating of STAT6
Zhanpeng SUN ; Xin LI ; Yan YANG ; Bo LI ; Hongyan WEI ; Chunlin HU ; Qingling FU ; Xiaoxing LIAO
Chinese Journal of Pathophysiology 2017;33(7):1301-1305
AIM: To explore the effect and possible mechanism of type 2 innate lymphoid cell (ILC2) on the development of chronic renal failure (CRF).METHODS: The patients with chronic renal failure (n=36) in the Fist Affiliated Hospital of Sun Yat-sen University from March 2016 to December 2016 were selected, and 32 healthy persons in the same period were enrolled in the study for control.The proportion of ILC2 in the PBMC of CRF patients and healthy controls was detected by flow cytometry, IL-13 concentration in the plasma was measured by ELISA.The isolated PBMCs from the patients and healthy persons were divided into 3 groups (control group, cytokine group, intervention group) and cultured in vitro for 3 days, respespestively, then IL-13 concentration was measured by ELISA.The protein levels of phosphorylated signal transducers and activators of transcription 6 (p-STAT6) in the PBMC of healthy controls before stimulation and after stimulation for 15 min, 30 min, 1 h, 2 h were determined by Western blot.RESULTS: The proportion of ILC2 in the PBMC and the plasma IL-13 concentration of CRF patients was higher than that in the healthy controls (P<0.05).In the culture supernatant in vitro, IL-13 concentration in the 3 subgroups of CRF patients (control group, cytokine group, intervention group) were all higher than that in the healthy controls (P<0.05), both the 2 groups showed a trend that the active IL-13 concentration in cytokine group was higher than that in control group, and that in intervention group was lower than that in cytokine group.The protein levels of p-STAT6 in cytokine stimulated-PBMC with a time dependent manner.CONCLUSION: The percentage of ILC2 in the PBMC is elevated in CRF patients.Furthermore, the ILC2 secret large amount of IL-13 to mediate the polarization of Th2 cells to regulate immunity through activating p-STAT6.
7.Clinical safety studies based on 30 026 post-marketing cases of Shenqi Fuzheng injection by intensive hospital monitoring nested NCCS.
Lian-xin WANG ; Yan-ming XIE ; Qing-hua AI ; Wen-fu XU
China Journal of Chinese Materia Medica 2015;40(24):4739-4745
This study adopted a large sample, multicenter, registered hospital centralized monitoring nested prospective case-control study design. From the real world clinical application of Shenqi Fuzheng injection, monitored 30 026 cases of patients with the use of Shenqi Fuzheng injection. A total of 51 adverse drug reaction (ADR) cases was monitored, including 1 case of severe adverse reactions. ADR incidence rate was 1.7 per 1,000. Blood samples were collected from patients with allergic reactions and their matched controls. Related biological indicators of allergic reactions were unified detected and analysed in order to explore the mechanism of allergic reaction and promote the clinical safety.
Case-Control Studies
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Drug Hypersensitivity
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etiology
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Drugs, Chinese Herbal
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adverse effects
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Humans
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Injections
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Product Surveillance, Postmarketing
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Prospective Studies
8.Study on Foxp3 promoter region methylation of mononuclear cells in patients with rheumatoid arthritis
Yiqun HAO ; Xiumei LIU ; Xin YAN ; Jie YANG ; Zili FU ; Dongping LUO ; Kai WANG
Chinese Journal of Rheumatology 2013;17(4):264-267
Objective By detecting the expression levels of Foxp3 in CD4+CD25+ regulatory T cells of peripheral blood mononuclear cells (PBMCs) in rheumatoid arthritis (RA),and the Foxp3 gene promoter region methylation to explore its role in the pathogenesis of RA.Methods Twenty-five RA patients and 10 healthy controls were selected,and the PBMCs were extracted by density gradient centrifugation.Foxp3 expression levels of CD4+CD25+ regulatory T cells were detected by flow cytometry.The real-time fluorescence quantitative PCR assay was used to detect the Foxp3 mRNA expression in PBMCs; and bisulfate processing gene sequencing was used to determinethe differences in Foxp3 gene promoter sequence methylation level of PBMCs.The comparison between groups was analyzed using one-way ANOVA; two sets of qualitative data were compared using Fisher's exact test.Results The expression levels of Foxp3 mRNA in the CD4+CD25+regulatory T cells of active RA patients (2.31±0.25) was significantly lower than inactive RA group (3.68±0.26) and healthy controls (5.67±0.34),the difference was statistically significant (P<0.05).The Foxp3 mRNA expression level in inactive RA group was lower than that of the healthy controls (P<0.05).Foxp3 promoter region-67,-74 sites of methylation level in PBMCs of RA patients (46%) was significantly higher than that of the healthy controls (6%).Conclusion Reduction in the number of CD4+CD25+ regulatory T cells may be involved in the pathogenesis of RA and Foxp3 gene promoter methylation levels plays a key role in this process.
9.Clinical examples of professor LI Zhi-dao's "tonifying three qi" acupuncture method.
Rui-Chao LI ; Yan LI ; Yuan-Xin FU ; Xiang-Fei ZHAO ; Jing SUN ; Lan-Yuan LI
Chinese Acupuncture & Moxibustion 2014;34(8):810-812
Professor LI Zhi-dao, according to acupoint selection of syndrome differentiation in TCM basic theory, concluded a new therapy, namely "tonifying three qi" that is mainly based on three acupoints in the Conception Vessel. This method is consisted of Danzhong (CV 17), Zhongwan (CV 12) and Qihai (CV 6) in the Conception Vessel, which could successively nourish clear qi, stomach qi and original qi. In clinic, according to the severity of symptoms of three qi, the acupoints are selected flexibly, which could respectively treat deficiency of heart-lung qi, deficiency of stomach-spleen qi and deficiency of original qi. Some examples are also given in the article.
Acupuncture Points
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Acupuncture Therapy
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history
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methods
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China
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History, 20th Century
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History, 21st Century
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Humans
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Qi
10.The role of Huang-qi in balance of THl/TH2 in asthma on dendritic cells level
Yan CHEN ; Zhou FU ; Kunhua CHEN ; Xiqiang YANG ; Enmei LIU ; Lijia WANG ; Xin LI
Chinese Journal of Microbiology and Immunology 2008;28(12):1064-1069
Objective To investigate the role of Huang-qi in balance of TH1/TH2 in asthma on dendritic cells level. Methods DCs from human peripheral blood mononuclear cells(PBMC) were induced by rhGM-CSF and rhIL-4, and then were identified. The level of IL-12 and IL-10 produced by DCs were de-tected by ELISA assay. After autoreactive T cells, mRNA of T-bet and GATA-3 was measured by RT-PCR. Simultaneously, IL-4 and IFN-γ were determined by flow cytometer. Results After 7 days culture, IL-12 was significantly decreased in asthma group compared to control group (P < 0.01), whereas IL-10 on the opposite. At the 7th day of co-culture with T cells derived from floating cells, the IFN-γ/and T-bet mRNA level in asthma group were significantly decreased than that in control group, whereas IL-4, GATA-3 mRNA level, the ratio of IL-4/IFN-γ and GATA-3/T-bet were apparently increased in asthma group than that in control group(P<0.01). After Huang-qi treatment, the IFN-γ/and T-bet mRNA level were significantly in-creased, whereas the ratio of IL-4/IFN-γ and GATA-3/T-bet, and the IL-10 level were apparently de-creased, but the level of IL-12, IL-4 and GATA-3 mRNA were not changed significantly. Conclusion DCs in asthma regulated the balance of TH1/TH2 by means of secreting decreased IL-12 and increased IL-10, that made TH2 playing a dominance role which is the key factor in initiating asthma. Huang-qi regulated DCs through decreasing the level of IL-10, and thus decreased the ability of inhibiting the differentiation of TH1 from TH0, that is also inhibiting the differentiation of TH2 from TH0 directly.