Objective:To find a better way to protect the security and interests of vulnerable groups by exploring the problem of protecting the vulnerable groups in drug clinical trial from the perspectives of ethics committees,organization and researchers.Methods:According to the relevant literature and the actual situation of the hospital,this paper analyzed the security issues of vulnerable groups comprehensively.Results:Only the ethics committees,organization and researchers work together,can it protect the security and interests of vulnerable groups to the greatest extents.Conclusion:Further research on the security of vulnerable groups not only promotes the development of human health,but also plays a decisive role in improving the protection of subjects in drug clinical trial.

Objective:To study the expression of p73? and its clinical significance in breast carcinoma.Methods:The expression of p73? was detected by immunohistochemistry in 41 breast carcinoma tissues,13 benign breast tumor tissues and 8 normal breast tissues.Results:The positive expression of p73? was found in 20/41(48.8%) of breast carcinoma tissues,1/13(7.7%)of benign breast tumor tissues,0/8(0)of normal breast tissues.The positive expression rate of p73? in breast carcinoma tissues was significantly higher than that in benign breast tumor tissues or normal breast tissues(P

This article summarized some in vitro models of drug absorption,whose shortcomings and virtues were compared in order to be convenient for researchers to know and select optimal model.

Objective:To explore the etiology and drug resistance of the ICU ventilator-associated pneumonia.Methods: One hundred cases of patients with ventilator-associated pneumonia received in our ICU room were selected; the pathologic examination results were retrospectively analyzed.Results: One hundred and thirty five bacteria were isolated from 100 cases of patients with ventilator-associated pneumonia, including 95 cases of Gram-negative bacilli(70.5%), 34 cases of Gram-positive cocci(25.1%) and 6 cases of fungi(4.4%); The resistance rates of Pseudomonas aeruginosa and Klebsiella pneumoniae for cefazolin, gentamicin, levofloxacin, and trimethoprim-sulfamethoxazole were high; The resistance rate of Staphylococcus aureus for ceftazidime, ceftriaxone, cefepime, cefazolin, gentamicin, amikacin, ciprofloxacin, levofloxacin, cefoperazone/sulbactam and piperacillin were high.Conclusion: ICU ventilator-associated pneumonia pathogen was mainly Gram-negative bacteria, and showed multidrug resistance, imipenem and cefoperazone/sulbactam for most pathogens had better efficacy and worthy of clinical application.

Cytochrome P450 is one of the microsomal mixed function oxidase,and plays an important role in the metabolism of exogenous and endogenous material.Lots of domestic and overseas relative literatures were red,the article summarized the research progress of traditional Chinese medicine affecting the metabolism of CYP in recent years.Eight main drug-metabolizing enzymes were respectively introduced,including CYP1A1,CYP1A2,CYP2A6,CYP2C,CYP2D6,CYP2E1,CYP3A4 and CYP3A4.It is important to research CYP450 for drug screening,individual administration.

Objective Tanycytes(TAs) is a specialized eppendymal glia that locates mostly in the ventral lateral wall of the ventricle III and median eminence(ME).Due to its peculiar location and directly exposure to the cerebrospinal fluid,blood,neuroendocrine hormones and neurons,tanycytes play an important role in the brain barrier system,brain-CSF neurohumoral circuit and immune-neuroendocrine network.They maintain immature characters during the adulthood and have naturally conducted the neuroregeneration process in the adult hypothalamus of mammal animals.This research was designed to identify tanycytes(TAs) in postnatal 7 days of Wistar rats and then establish the cell model of TAs for further study.Methods Tanycytes of postnatal 7 days of Wistar rats were identified by the immunohistochemical technology using glial fibrillary acidic protein(GFAP) and vimentin(VIM),which are the intermediate filament markers of glia cells.The qualitative analysis was performed by the NADPH-d stain of nitric oxide sythenase(NOS).Primary TAs model was established by the cell culture technique and identified by the same methods as in vivo.Results Primary cultured TAs expressed VIM,GFAP and NOS which was identical with those in vivo.Conclusion Cell model of TAs from postnatal 7days of Wistar rats in vitro has been established and TAs could be used as a proper substrate for transplanting.

Type 1 diabetes mellitus (TlDM) is an autoimmune disease characterized by the destruction of pancreatic β cells, which sequentially leading to the reduction of insulin secretion. Numerous susceptibilities, including genetic and environmental factors, may be involved in triggering the T1DM. The ideal therapy for T1DM should focus on both preventing the immune system from attacking β cells, and also the stimulation of β cell neogenesis. In vitro and in vivo studies both demonstrated that GLP-1 analogues were able to promote the synthesis and secretion of insulin, stimulate neogenesis of β cells, increase β cells replication, and reduce β cell apoptosis. Hence, GLP-1 analogues have been shown to be potential treatment for T1DM. This work reviewed recent publications regarding to GLP-1 application in TlDM and pancreatic islet transplantations and prospected the future research directions.

Reviews research findings about aberrant crypt foci on concepts, relation to colon carcinogenesis, relative genes and biomarkers, methods, signification. It shows that aberrant crypt foci are possible precursors of adenoma and cancer, which can be early diagnosis marks of colon carcinogenesis.

OBJECTIVE: To evaluate the utilization and its trend of narcotic analgetics in this hospital.METHODS: The use of narcotic analgetics during the period 2005-2007 in our hospital was analyzed retrospectively.RESULTS: The consumption quantity of narcotic analgetics in our hospital increased year-on-year,much as in the consumption of morphine preparations,with oral morphine preparations dominating the 3 first places over the 3 years,however,the increase of pethidine was slowed down in consumption quantity.CONCLUSION: The use of narcotic analgetics in our hospital is rational on the whole,but measures should be taken to tighten the control on the use of narcotic analgetics.