Objective　To study the change and clinical implications of tumor necrosis(TNF-α) and interleukin-6(IL-6) in the lung cancer patients. Methods　Serum TNF-α and IL-6 level in 55 of lung cancer cases and 40 in normal control group were assayed by ELISA. Results　Serum TNF-α and IL-6 level were significantly higher in the lung cancer patients than in normal control(P<0.01). The activity of TNF-α and IL-6 in serum of the no operational patients (with lymphanode matastasis) was higher than the recurrent patients of post-operation (P<0.01). Conclusion　The activity of serum TNF-α and IL-6 may be associated with development of the lung cancer. The determination of TNF-α and IL-6 levels in serum can help to observe the lung cancer state as a assistant monitor methods.

The number of chemicals that are synthesized each year has been steadily increasing. Chemicals are of immense benefits to mankind,many of which,however,have a significant negative impact on the environment as well as human health primarily due to their inherent chemistry and toxici?ty. Human exposure to numerous toxic chemicals takes place not only in the environment and but also in the workplace. Therefore,new approaches to toxicology need to developed. A milestone in this regard is the versions of the US National Research Council ″Toxicity Testing in the 21st Century:a Vision and a Strategy ″ and ″Science and Decisions:Advancing Risk Assessment ″. The versions require a radical paradigm shift in the approach to safety assessment and in the traditional procedure where animal experiments used to be the dominating technology. The future of toxicology suggests a new conceptual framework within which in vitro and in silico approaches based on human materials are used to under?stand disease pathways at multiple biological levels that are analogous to adverse outcome pathways in toxicology. This paper focuses on the many challenges and issues that need to be solved in this area.

The incidence of allergic diseases, including bronchial asthma, atopic dermatitis, allergic rhinitis and food allergies, is increasing year by year.Recently, more and more studies have been conducted to explore the relationship between intestinal microecology and allergic diseases, which indicated that the influence of intestinal flora on the immune system is one of the important mechanisms.This article was based on the immune status and microecology.This article reviewed and explored the important role and mechanism changes of intestinal flora in the early life in terms of the occurrence and development of allergic diseases, and the research progress in the application of probiotics in allergic diseases, aiming to further understand the relationship between intestinal microecology and children allergy and provide new ideas for the prevention and treatment of allergic diseases.

The traditional Chinese medicinal therapy of the eye acupuncture is one of superior microacupuncture therapies which was invented by famous acupuncture professor Peng Jing-shan. Through needling points around the eye, the treatment can treat the diseases of whole body, especially for curing apoplectic paralysis. This article expounded and analyzed the mechanism of eye acupuncture in treating apoplectic paralysis through medical theory, biological holographic theory, modern nerves anatomy and nerves physiology.

One hundred medical undergraduate interns were randomly selected to undergo a survey using self-designed questionnaire,95 students completed the questionnaire with a response rate of 95.0％.The results revealed that all interns receiving survey had psychological adaptability problems,57.9％ of the interns were not adaptive to life well,67.7％ had maladjustment of learning,63.2％ had social maladjustment,65.3％ had poor metal adaptability.The 47.4％ of the interns felt very heavy stress and 36.8 ％ felt stress,only 15.8 percent felt less stress.The stress sources included seeking employment and pursuing career for clinical practice or academic training.The results showed that psychological adaptability is a common problem for medical undergraduate interns and the social stress sources would intervene the clinical training and study for them.The medical educators should pay attention to this problem and psychological counseling and other measures should be taken for medical interns.

10 cm respectively from the tumor),the metastatic rate of lymph nodes was 34.7%,2.5% and 1.7%,respectively.The metastatic rate of LN in right colon was significantly higher than that in left colon(52.0% vs 33.8%,P

OBJECTIVE:To explore the effects of Compound Ligusticum chuanxiong drop pill on hemorheology and blood gas in model rats with diabetic nephropathy(DN). METHODS:60 rats were randomly divided into normal control group,model group, positive control group [Irbesartan tablet,0.013 g/(kg·d)] and Compound L. chuanxiong drop pill high-dose,medium-dose,low-dose groups [0.4,0.2,0.1 g/(kg·d)],10 in each group. Except for normal control group,rats in other groups were given high-sugar high-fat feed and intraperitoneally injected streptozotocin to reduce DN model. After 7 d modeling,rats in each group received relat-ed drugs intragastrically,once a day,10 mL/kg;normal control group and model group received equal volume of purified water in-tragastrically. After 64 d,hemorheology indicators [whole blood viscosity(high-shear,medium-shear and low-shear),plasma viscosi-ty,whole blood reduced viscosity] and blood gas indicators(carbon dioxide partial pressure,oxygen partial pressure,hemoglobin content,oxygen saturation,ratio of oxygenated hemoglobin to total protein) of rats were determined,hematoxylin-eosin staining was used to observe the pathological changes in kidney tissue. RESULTS:Compared with normal control group,hemorheology indi-cators and carbon dioxide partial pressure in model group were significantly increased(P<0.05 or P<0.01),oxygen partial pres-sure,hemoglobin content,oxygen saturation,ratio of oxygenated hemoglobin to total protein were significantly decreased(P<0.05 or P<0.01),with severe kidney damage. Compared with model group,except the improvement of carbon dioxide partial pressure, oxygen partial pressure,hemoglobin content in rats were not significant in Compound L. chuanxiong drop pill low-dose group,the above-mentioned indicators in other medicine groups were significantly improved(P<0.05 or P<0.01);the kidney tissue damage was improved to varying degree. CONCLUSIONS:Compound L. chuanxiong drop pill can significantly improve the hemorheology indicators of DN model rats,reduce blood viscosity,improve microcirculation and capacity of the blood to transport oxygen,and has certain improvement effect on kidney tissue morphology of DN rats.

Wilms' tumor is the most common kidney tumor in childhood.MicroRNAs (miRNAs) are about the size of 19-22 nucleotide sequences that exist widely in the non-coding RNA in eukaryotes,function primarily through combination with mRNA target base pairing causing target mRNA degradation or inhibition of translation,and then develop his inhibiting or promoting the role of tumor.Abnormal expressions of miRNAs can cause a lot of kidney diseases,such as chronic kidney disease,polycystic kidney disease,renal fibrosis and renal cancer.Wilms'tumor,abnormal expression of key genomes,such as miR-17-92,miR-185,miR-204,miR-48 anomalies and tumors are closely related.Paper of miRNAs in incidence of Wilms'tumor expression,and the possible role for future targeted gene targeted drug sites are reviewed.

It is of great importance that deep learning of computer for the automate identification of the two-dimensional image of Chinese herbal slices is valuable in the application to medicine,production and education.Traditional methods usually extract low-level image features for the identification,but they cannot give robust recognition results under complex backgrounds.Therefore,higher level image representation is necessary in the image identification.A public Chinese herbal medicine database was constructed with 50 common categories and 2,554 images in total,for training and evaluating our recognition model.Then,the sofimax loss function was adopted to train the convolutional neural network model.As a result,the convolutional neural network can achieve the average precision of 70％ under all the 50 medicine herbal classes.In conclusion,convolutional neural network can obtain good results in image identification with complex backgrounds and mutually occluded herbal slices,which has promising potential for future applications.