AIM:To establish the method for quality control of Jueming Jiangzhi Tablet(Semen Cassiae,Radix Polygoni multiflori,Herba Taxilli,Herba Artemisiae scopariae,etc.).METHODS:Radix Polygoni multiflori、 Herba Taxilli、 Herba Artemisiae scopariae、Semen Cassiae were identified by TLC.HPLC method for determining emodin and 2,3,5,4'-tetrahydroxydiphenylethylenic-2-O-?-D-glucoside in Jueming Jiangzhi Tablet were established.A Dikma Diamonsil C_ 18 column was used,the mobile phases were methanol-0.1% phosphate solution(85∶15)and acetonitrile-water solution(5∶15),respectively.The flow rate was 1.0 mL/min.The detection wavelength was at 254 nm and 320 nm,respectively.RESULTS:The spots on the TLC plate were clear.The negative control sample didn't disturb.A good linearity was obtained for emodin in the range of 0.131 2-1.312 0 ?g,r= 0.999 8.The average recovery was 99.80%,RSD=1.17%(n=6).A good linearity was obtained for 2,3,5,4'-tetrahydroxydiphenylethylenic-2-O-?-D-glucoside in the range of 0.580 0-5.800 0 ?g,r=0.999 8.The average recovery was 98.68%,RSD=1.36%(n=6).CONCLUSION:The methods are specific,accurate and reproducible,and can be used for the quality control of the product.

Humans ; Lymphoma, Large B-Cell, Diffuse ; diagnosis ; therapy

Objective] To summarize professor Shen Hong's clinical experience in treating chronic atrophic gastritis. [Method] By following the teacher clinic and sorting out the related medical materials, the author summarizes professor Shen Hong's academic experience of treatment of chronic atrophic gastritis, and for proven cases. [Result] Professor Shen Hong thinks that the essence of pathogenesis is the weakness of the spleen and stomach. Qi stagnation, damp stagnation and blood stasis are common pathological factors. The characteristic of the disease is deficiency in origin and excess in superficiality. The location of disease is in stomach, relating to liver and spleen. The principle of treatment should be strengthening the spleen and stomach. It is also very important to treat accompanying symptoms and balance property of Chinese medicines. Prof. Shen emphasizes the combination of disease and syndrome differentiation and the integration of TCM and western medicine. He attaches much weight to diet and psychotherapy. The medication is concise and the prescription changes flexibly with the permitted addition and subtraction. The clinical curative effect is satisfactory. [Conclusion] Professor Shen Hong 's experience is effective and worthy of inheritance and promotion.

With the development of the Multi-Rigid-Body system dynamics theory and computer technology, computer aided design and manufacturing of orthosis have already matured, mathematical mechanical model of human body and orthosis can be established using this new technology, and simulation calculation can be used to determine the orthosis prescription, predict the efficacy and effectiveness of rehabilitation. Early application of ankle-foot orthosis plays an important role in restoration of brain function in stroke patients, resumption of hemiplegic gait and abnormal gait correction. In clinical orthosis prescription, establishment of corresponding mathematical model and speculation of ankle-foot function changes, as well as the effect and mechanism of resumption of brain function in post-stroke patients, have a positive impact on the analysts of hemiplegic gait, orthosis design and gait resumption.

0.05). Conclusion The results suggest that there is autoimmunological mechani sm in the pathogenesis of psoriasis.

OBJECTIVE: To evaluate the utilization and its trend of narcotic analgetics in this hospital.METHODS: The use of narcotic analgetics during the period 2005-2007 in our hospital was analyzed retrospectively.RESULTS: The consumption quantity of narcotic analgetics in our hospital increased year-on-year,much as in the consumption of morphine preparations,with oral morphine preparations dominating the 3 first places over the 3 years,however,the increase of pethidine was slowed down in consumption quantity.CONCLUSION: The use of narcotic analgetics in our hospital is rational on the whole,but measures should be taken to tighten the control on the use of narcotic analgetics.

Objective To determine the level of anticyclophilin A auto-antibodies (ACA) in sera of patients with psoriasis. Methods Cyclophilin A (CyPA) was extracted and purified from bovine thymus. The level of anticyclophilin A auto-antibodies (ACA) was detected by enzyme-linked immunosorbent assay (ELISA) and immunoblotting test (IBT) in sera of patients with psoriasis. Results It was shown that the level of ACA was significantly higher in the sera of patients with psoriasis than those in control group (IBT:P<0.01,ELISA:P< 0.01). ACA was found to be positive in 41.3％ of the patients by ELISA. In psoriatic group, the serum level of ACA was higher during the progressive stage than that in the stationary stage, however, no significant difference was observed (IBT:P>0.05,ELISA:P >0.05). Conclusion The results suggest that there is autoimmunological mechanism in the pathogenesis of psoriasis.

Objective: To investigate the association of polycystic ovary syndrome (PCOS) with plasminogen activator (tissue-type plasminogen activator [t-PA], urokinase-type plasminogen activator [u-PA]) and plasminogen activator inhibitor-1 (PAI-1). Methods: The ovary tissues of 30 PCOS patients who were treated in our hospital from Dec. 2007 to Oct. 2008 and 20 healthy controls were collected. The expression of t-PA, u-PA, and PAI-1 was examined by immunohistochemistry method. Results: In PCOS group, the positive rates of u-PA, t-PA in the granulosa cells were 20.00% and 26.67%, and in the theca cells were 33.33% and 20.00%, respectively; in the control group, the positive rates of u-PA, t-PA in the granulosa cells were 65.00% and 75.00%, in the theca cells were 75.00% and 50.00%, respectively; and there were significant differences between the two groups (P<0.05). In PCOS group, the positive rate of PAI-1 was 56.67% in the theca cells and 63.33% in interstitial cells; in the control group, the positive rates of PAI-1 were 10.00% both in the theca cells and the interstitial cells; and there were significant differences between the two groups (P<0.05). Conclusion: The disruption of plasminogen activator system may be involved in ovulation disorder of PCOS.

To study and observe the changes of serum, aqueous humor and tear lL - 2, slL - 2, NO, TAC, and SOD of patients with cataract.
●METHODS: Totally 60 patients with cataract in our hospital from November 2011 to September 2013 were included as the observation group, and 60 patients with eye traumas were as the control group. The serum, aqueous humor and tear lL - 2, slL - 2, NO, TAC, and SOD of two groups were detected and compared, and the detection levels of patients with different stages were compared.
●RESULTS: The serum, aqueous humor and tear lL-2, TAC, and SOD of observation group were all lower than those of control group, the slL-2 and NO were all higher than those of control group. The serum, aqueous humor and tear detection levels of patients at different stages had obvious differences, and the aqueous humor lL - 2 were lower than those of serum and tear, the slL-2 were higher than those of serum and tear, with statistically significant differences.
●CONCLUSlON: The change of serum, aqueous humor and tear lL-2, slL-2, NO, TAC, and SOD of patients are all great, and the influence of the stages for those indexes are great too, the inflammatory response state of aqueous humor are more obvious.

BACKGROUND:Preadipocyte is a kind of specific precursor cells with the potential of proliferation and differentiation into adipocytes.If factor or drug with strong promotion of preadipocyte proliferation and differentiation is obtained and used in adipose tissue transplantation,which could elevate transplantation outcome of adipose tissues.OBJECTIVE:To find an optimal culture environment and to accumulate culture experience of in vitro culture 3T3-L1 preadipocytes,and to observe the effect of insulin-like growth factor(IGF)-1 on the proliferation and differentiation of 3T3-L1 preadipocytes,and to explore the optimal concentration of IGF-1.DESIGN,TIME AND SETTING:The cell control observation was performed at the Science Experiment Center,Liaoning University of Traditional Chinese Medicine from October 2007 to February 2008.MATERIALS:3T3-L1 preadipocytes were purchased from Shanghai Institutes For Biological Sciences.IGF-1 was bought from Biological,USA.METHODS:According to in vitro culture direction of 3T3-L1 preadipocytes,3T3-L1 preadipocytes were divided into 6 groups.3T3-L1 preadipocytes in the blank control group were inoculated in high-glucose DMEM supplemented with 0.1 volume fraction of fetal bovine serum.3T3-L1 preadipocytes in each experimental groups were respectively treated with high-glucose DMEM containing 5,10,20,50,100 ?g/L IGF-1 and 0.1 volume fraction of fetal bovine serum.MAIN OUTCOME MEASURES:Growth,proliferation and differentiation of 3T3-L1 preadipocytes in each experimental groups under the inverted microscope,and photographed.When cells were confluence,cell cycles were detected by flow cytometry.Growth rate of 3T3-L1 preadipocytes was measured by MTT essay.Fat growth rate in 3T3-L1 preadipocytes were examined by using oil red O stained-extracted assay.RESULTS:Inverted microscope showed that 3T3-L1 preadipocytes before differentiation were fusiform shape,with little fat in their endochylemas.3T3-L1 preadipocytes after differentiation were spherical shape,with much fat in their endochylemas.Lipid droplet gathered in the perinuclear space,and was stained orange by oil red O stained-extracted assay,forming ring-shape.Results from MTT essay and oil red O stained-extracted assay demonstrated that growth rate of 3T3-L1 preadipocytes and fat growth rate in 3T3-L1 preadipocytes were higher in each experimental groups treated with different doses of IGF-1 compared with the blank control group(P