Objective To investigate the possibility of reserving uterus during severe postpartum hemorrhage. MethodsA retrospective analysis was conducted on the clinical data of 138 cases of severe postpartum hemorrhage (blood loss ≥2000 ml) from January 1, 2003 to December 31, 2009. Results Among 138 cases of severe postpartum hemorrhage, uterine atony (n= 60, 43. 38 ％ ) was the first cause and the second was placental factor (n= 55, 39.86 ％). The blood loss varied from 2000 ml to 10 000 ml and the mean level was about (3004± 1473) ml. The volume of blood transfusion for these patients varied from 800 ml to 7200 ml. Among these patients, the blood loss of 108 cases reserved uteri was from 2000 ml to 7500 ml, with the average of (2564±932) ml； while for 30 cases performed with hysterectomy, the blood loss was about 2500 to 10 000 ml averagly (4653± 1857) ml (t=8. 57, P=0.00). These patients were divided into two groups according to time series. Twelve cases of hysterectomy were performed during 2003to 2005, and the hysterectomy rate was 0. 47‰; 18 cases of hysterectomy were performed during 2006 to 2009, and the hysterectomy rate was 0. 36‰. The average blood loss of the above two groups was (3783±861) ml and (5233±2124) ml respectively (t=2. 234, P=0. 034). Among all the cases with blood loss ≥ 3000 ml, uteri were reserved in 24 cases with the average blood loss of (3818 ± 1284) ml； while hysterectomy were performed in 27 cases with average blood loss of (4900 ± 1789) rnl (t = 2. 453, P =0. 018). The time for blood loss to 3000 ml in the two groups was (160±129) min and (100±67) min,respectively, and the difference was significant (t=2. 113, P = 0.04).The uteri of six cases with postpartum hemorrhage over 4000 ml were successfully reserved, and average bleeding amount was 5570 ml.Two patients among the 138 women died of amniotic fluid embolism.The perinatal mortality rate was 3. 73％.ConclusionsThe postpartum hemorrhage volume and velocity is the key point to decide whether to reserve the uterus or not.For the population with high risk factors,prophylaxis treatment with prostaglandins should be initiated to reduce the bleeding volume.Uterine packing might be an effective treatment to stop postpartum hemorrhage, especially for those bleeding due to placenta previa.

The development of clinical microbiology laboratory is an essential part in promoting the development of clinical microbiology and the basis rational use of antimicrobial agents.Under the circumstances of national specific rectification of antimicrobials,clinical microbiology laboratory should focus on self-buildup and improvement.The development of clinical microbiology laboratory should focus on development of both software and hardware capabilities for lab examination and diagnosis in an all-round way,enhance the antimicrobial susceptibility surveillance,and emphasize and communication and cooperation between laboratory and other departments e.g.clinical departments,pharmacy department and nosocomial surveillance and control office,making concept translation from past ‘paramedical department' to a ‘platform department',contribute its due share to the rational uses of antimicrobial agents.

This study was aimed to reveal the effector mechanisms of Chinese medicine aconite, ginseng, astragalus and dried ginger on the intervention of adriamycin (ADR) cardiotoxicity model rats. The analysis was made on the interactive relationship between aconite and ginseng, astragalus as well as dried ginger. A total of 72 rats were randomly divided into nine groups. There were eight rats in each group. Except the normal group, rats in each group were intraperitoneally injected with 2.5 mg·kg-1 of ADR according to their body weights. The injection was given once a week and continued for four weeks. The total dosage was 10 mg·kg-1. In the aconite, dried ginger group, the intragastric administration dosage of herbal decoction was 1.75 g·kg-1. The decoction dosage in the gin-seng, astragalus group was 0.875 g·kg-1. The decoction dosage in the Shenfu, Qifu group was 2.625 g·kg-1. The decoction dosage in the Jiangfu group was 3.5 g·kg-1. The intragastric administration was given once a day and continued for four weeks. Indexes such as superoxide dismutase (SOD), cardiac troponins (cTn), cytochrome C (CytC), myocardial mitochondria of Bax, Bcl 2, caspase-3, caspase-9 were detected. The colligation score was calculated associating with the close index. One-way ANOVA was given on different indexes and colligation indi-cators among different drug groups and the factorial design variance analysis was given to reveal the drug interac-tions. The results showed that compared with the normal group there were statistical significances among different indexes in the model group (P < 0.05). Aconite, ginseng, astragalus, dried ginger had varying degrees of impact on different indicators. There were statistical significances on the interaction between aconite and ginseng, astra-galus, dried ginger (except Bax). It was concluded that herbal medicine aconite, ginseng, astragalus, dried ginger had certain protective effect to the heart of ADR model rats. The combination of aconite and ginseng, astragalus, dried ginger can enhance the effect compared with a single herb.

Objective To observe the clinical curative effect of BudesonideNasal Spray plus Gelomyrtol Forte for acute secretory otitis media.Methods 80 Patients (108ears) and 60 patients (74ears) were treated by BudesonideNasal Spray be bound to Gelomyrtol Forte and Cefaclor Dispersible Tablets res pectively. After 10 days, observe the two groups’s clinical curative effect for acute secretory otitis media.Results The of treatment’s group efficient rate is 99.07, Cefaclor Dispersible Tablets 87.84, and the difference is (?2 =10.68 P0.05), and mild adverse reactions will be disappeared after stoping drugs. Conclusion The of BudesonideNasal Spray puls Gelomyrtol Forte is better than that of Cefaclor Dispersible Tablets for acute secretory otitis media. The mild adverse reaction is the same between the two groups.

AIM: To investigate the clinical effects of phacoemulsification and surgical techeniques in small pupil cataract after filtering operation of glaucoma.
METHODS: Thirty-six cases ( 36 eyes ) of small pupil cataract after filtering operation of glaucoma were underwent phacoemulsification combined with foldable intraocular lens implantation. Postoperative visual acuity, intraocular pressure filtration bleb and complications were observed. The follow-up time was 3mo.
RESULTS: The preoperative uncorrected visual acuity (UCVA) or best-corrected visual acuity ( BCVA) ≤0. 3 were in all patients. Postoperativerly, the UCVA or BCVA≥0. 3 after 1wk and 3mo were achieved in 23 eyes (63.89%) and 34 eyes (94. 44%). BCVA was <0. 3 in 2 eyes ( 5. 56%) including optic nerve atrophy of one eye and diabetic retinopathy of one eye. All patients were kept the level of normal intraocular pressure and completed filtering bleb, there were no serious complications.
CONCLUSION: Phacoemulsification in small pupil cataract after filtering operation of glaucoma was complex, and reasonable application of operation skills can still achieve better clinical results. The main factor of affecting the visual acuity is pre-existing retinal optic neuropathy.

It had fertile countryside land,rich products,dense population and creeks and lakes in ancient Hangzhou and its neighbour area,where there is a good place for planting medicinal materials.It had long history of planting medicinal materials with abundant output,pure and good nature,and much varieties;among which,some famous and costly medicinal materials,such as "eight Zhe's" and "eighteen Hangs",were not only adopted broadly by court,royalty and doctors,but purchased by many businessmen and sold far to abroad as well;they seemed to be in the leading place in China.

Objective: To establsh the quality standard for Fufang Jiangya liniment (Folium Apocyni Veneti, Semen Cassiae, Semen Vaccariae, etc.). Methods: Semen Cassiae, Folium Apocyni Veneti and Semen Vaccariae were identified by TLC. Fructus Coriandri was identified by GC. Chrysophanol and quercetin were determined by RP HPLC. Results: The petroleum ether (boiling range: 30～60?C) n hexane ethyl acetate formic acid (1∶3∶1.5∶0.01) was used as the developer for the identification of Semen Cassiae on silica gel G coating plate. The toluene ethyl acetate formic acid (5∶4∶1) was used as the developer for the identification of Folium Apocyni Veneti and Semen Vaccariae, respectively, also on silica gel G coating plate. By GC, licareol as standard substance, Fructus Voriandri could be identified. By RP HPLC, kromasil C 18 as fixed phase, when methyl alcohol water perchloric acid (80∶20∶0.1) was used as the mobile phase. ( ? =257nm), the average recovery of chrysophanol was 0.0298mg?mL -1 , methyl alcohol 0.5% phosphoric acid solution (1∶1) was used as the mobile phase ( ? =370nm), the average recovery of quercetin was 1.1522mg?mL -1 . Conclusion: The methods established are simple, feasible and reproducible and can be used as the quality standards for Fufang Jingya Liniment.

OBJECTIVE To set up a kind of Helicobacter pylori(Hp) detection method which can determine the Hp infection and its clinical value for the digestive diseases.METHODS Gastric biopsy was used with gastric mucosa Hp rapid urease test,Hp-IgG was detected by ELISA,and Hp-DNA of feces by fluorescent gene quantitation method.RESULTS From the 96 patients positively determined by gastric biopsy,the positive rate of Hp-DNA of feces was 95.8%,but the positive rate of Hp-IgG was 47.9%.From the 59 Hp-IgG positive patients,the positive rate of gastric biopsy was 45.8%.CONCLUSIONS Gastric biopsy and HP-DNA quantitation of feces are both reliable methods which can determine the Hp infection,but Hp-DNA quantitation of feces is a simple,rapid,reliable and atraumatic test,it can be put into practice widely and more easily.

Cell adhesion molecules(CAMs) play important roles in specifying cell-cell interactions during development,regeneration,and modification of synaptic activity.The close homolog of L1(CHL1) ,a recently identified member of the immunoglobulin superfamily of cell adhesion molecules,is localizably expressed in the nervous system.CHL1 interacts with like molecules(homophilic interaction) and non-like molecules(heterophilic interaction) on neighboring cells or the extracellular matrix to regulate axon outgrowth and fasciculation,neuronal migration and survival,synaptic plasticity and regeneration after trauma.

Objective To study the impact of sodium arsenite(NaAsO2) exposure on melanoma cells A375 (hereinafter referred to as the A375) and G361 (hereinafter referred to as the G361) pigment production and tyrosinase (TYR) activity and the differences of pigment metabolism capacity between the cell lines.Methods A375 and G361 cells were exposed to sodium arsenite at concentrations of 0.0(control),0.1 and 1.0 μmol/L for 72hours.Cell viability was measured by Alamar Blue assay.Melanin levels and TYR activity were measured at the same time.Results After exposure for 72 hours,the cells of 0.1 μmol/L dose groups of both of the two cell lines [A375:(103.32 + 1.26)％; G361:(104.10 + 1.76)％] showed a slightincrease of proliferation without significant differences compared with those of the control[A375:(100.00 ± 1.08)％; G361:(100.00 + 1.79)％,all P ＜ 0.05] ;while cell viability of the 1.0 μmol/L dose group of both of the two cell lines[A375:(75.32 ± 1.59)％; G361:(78.26 ± 2.10)％] were significantly lower than those of the control (all P ＜ 0.05).Melanin levels of G361 cell line [(7.19 ± 0.35),(7.34 ± 0.83),(8.19 ± 0.86)pg/cell] were significantly higher than that of A375[(4.35 ± 0.72),(4.54 ± 0.01),(4.60 + 0.59)pg/cell,all P ＜ 0.05] in all the three groups.TYR activity of G361 cell line [(54.13 ± 1.21),(54.56 ± 0.21),(56.25 ± 0.85)Bq] were also markedly higher than that of A375 cell[(42.00 ±0.21),(42.90 ± 0.54),(42.91 ± 0.01)Bq,all P ＜ 0.05] in all the three groups.The melanin levels and TYR activities of both of the two cells lines showed an increase tendency along with increased doses of arsenic exposure,but without significant differences when compared with those of the three groups (all P ＞ 0.05).Conclusions Arsenic related pigment disorder may be associated with increased melanin levels and TYR activities induced by arsenic exposure; individual difference of pigment metabolism may be associated with different basal melanin levels and TYR activity between different individuals.