There were significant increase in urine protein excretion,raised malondialdehyde(MDA) level and expressions of NF-?B,p22phox and p47phox in renal tissue,and significant decrease in reduced glutathione,superoxide dismutase,vitamin C and E levels in type 2 diabetic Goto-Kakisaki rats after 12 weeks. There was obvious histomorphologic change in the kidneys.All the above indices were improved by intraperitoneal injection of?-lipoic acid(35 mg/kg q.o.d).Besides,significant positive correlations were found of MDA level to p22phox,p47phox and NF-?B in the renal tissue,?-lipoic acid seems to protect the diabetic kidney in this diabetic rat model via antioxidative effects.

0.01),which were significantly different from group Ⅲ(P

Objective To study the effect of early rehabilitation with full range of music therapy on acute coronary syndrome (ACS) patients after emergency PCI. Methods From July to December 2009, 108 inpatients undergone emergency PCI were randomly divided into experimental group and control group, with 54 patients in each group. The experimental group received full range of music therapy, which included PCI treatment under the circumstance of music and music therapy 3 times per day (10:00 a. m. , 16:00 p. m. and 21:00 p. m. ) during hospitalization. The control group received conventional treatment. SBP, HR, the left ventricular ejection fraction (LVEF) , 6-MWT, length of stay, the scale of anxiety and depression were compared between the two groups. Results The scores of anxiety and depression, SBP, HR, LVEF and 6-MWT in the experimental group were significantly different from those of the control group ( P < 0. 05). Conclusions Full range of music therapy significantly contributes to the rehabilitation of ACS patients after emergency PCI.

OBJECTIVETo evaluate and analyze the clinical therapeutic effect and characteristics of acupuncture combined with medicine on depression of different syndrome types.

METHODSEighty cases of depression were dividede into liver qi stagnation and spleen deficiency group (group A, 35 cases), deficiency of both heart and spleen group (group B, 21 cases) and yang deficiency of both spleen and kidney group (group C, 24 cases) according to the syndrome differentiation. They were all treated with acupuncture at Baihui (GV 20), Yintang (EX-HN 3), Sishen-cong (EX-HN 1), Neiguan (PC 6) etc. and oral administration of 5-HT selective serotonin reuptake inhibitors (SSRI) tablets. The Hamilton depression scale (HAMD) and self-made syndrome differentiation symptoms evaluation scale were adopted to evaluate the therapeutic effect.

RESULTSAfter treatment of 4 weeks, the total effective rate of 91.4% in group A and of 90.5% in group B, both were superior to that of 87.5% in group C (P < 0.01,P<0. 05). There was a significant difference in comparison of HAMD scores between the group A and group C after treatment of 4 weeks (P < 0.05). The symptom scores of syndrome differentiation in all three groups were obviously decreased after 1 week, 2 weeks and 4 weeks treatment compared with those before treatment (all P < 0.001), the symptom scores of group C had significant differences compared with the group A and group B after treatment of 2 weeks (P < 0.01, P < 0.05). The reducing scores rate of the group A had singificant differences compared with the group B and the group C after treatment of 4 weeks (both P < 0.05).

CONCLUSIONAcupuncture combined with SSRI has better therapeutic effect on depression of liver qi stagnation and spleen deficiency type. The syndrome differentiation symptoms evaluation scale is more exact and sensitive to evaluate the clinical therapeutic effect of TCM treatment.

Acupuncture Therapy ; Adult ; Aged ; Aged, 80 and over ; Combined Modality Therapy ; Depression ; drug therapy ; therapy ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Serotonin Uptake Inhibitors ; therapeutic use ; Young Adult

OBJECTIVETo detect the expressions of miR-122 and miR-224 in hepatocellular carcinoma (HCC) by real-time fluorescence quantitative RT-PCR and investigate the significance of miRNAs in early diagnosis of HCC.

METHOD2(-Delta Delta CT) method was used for quantitative analysis of the expression pattern of miR-122 and miR-224 in 35 HCC and adjacent normal tissues. All the quantitative results were confirmed by Northern blotting.

RESULTSCompared with adjacent normal tissues, the HCC tissues showed significant miR-122 down-regulation (P<0.01) and miR-224 over-expression (P<0.001).

CONCLUSIONSHCC has obvious alteration in the expression patterns of miR-122 and miR-224, and real-time fluorescence quantitative RT-PCR provide a new means for early, efficient, and accurate diagnosis of HCC.

Adult ; Aged ; Base Sequence ; Blotting, Northern ; Carcinoma, Hepatocellular ; diagnosis ; genetics ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Liver Neoplasms ; diagnosis ; genetics ; Male ; MicroRNAs ; genetics ; Middle Aged ; Reverse Transcriptase Polymerase Chain Reaction ; Spectrometry, Fluorescence ; Time Factors

OBJECTIVETo describe the incidence and mortality rates of esophageal cancer from 1974-2002 in Cixian county of Hebei province. Basic information on comparative geographical, epidemiological, and clinical research was collected.

METHODSIn early 1970s, cancer registry system in Cixian was established, collecting information on all the esophageal cancer cases in Cixian. Data was checked manually, then computerized, coded and analyzed using the software--SPSS 11.5.

RESULTSFrom 1974 to 2002, there were 18 471 esophageal cancer cases in Cixian, with 11 068 males and 7403 females, respectively. The age standardized incidence rate (ASR) for males was 208.77 per 100,000, while 120.47 per 100,000 for females. The trend of incidence rate of esophageal cancer had decreased during the 29 years from 1974 to 2002 (trend chi(2) = 19.94, P < 0.001). From 25 years of age onward, the incidence rates of the lower age groups declined with the increase of age. As for geographic distribution, the incidence rate in mountainous areas and hilly areas showed a significant declining trend in mountainous areas, chi(2) = 195.00, P < 0.001; hilly areas, chi(2) = 46.08, P < 0.001. The esophageal cancer incidence in plain areas remained steady, but had a slight increase in recent years. From 1969 to 2002, there were 18,736 cases died of esophageal cancer with 11 598 males and 7138 females. The ASR for male was 127.17 per 100,000 and 101.57 per 100,000 for female. Compared with the year 1969, the mortality rate of esophageal cancer in 2002 had a 37.96% decline. The proportion of esophageal cancer among malignant tumors in different decades decreased significantly.

CONCLUSIONThe trend of the incidence rate of esophageal cancer had been decreasing for the last 29 years. The incidence rate in mountainous areas and hilly areas showed a declining trend while in the plain areas it remained steady but having slight increase in the recent years. The mortality rate of esophageal cancer had a significant decrease from 1969 to 2002.

Adult ; Age Factors ; China ; epidemiology ; Esophageal Neoplasms ; epidemiology ; mortality ; Female ; Humans ; Incidence ; Male ; Registries ; Software

Adenocarcinoma ; metabolism ; pathology ; surgery ; Adult ; Aged ; Aged, 80 and over ; Carcinoembryonic Antigen ; metabolism ; Carcinoma, Neuroendocrine ; metabolism ; pathology ; surgery ; Chromogranins ; metabolism ; Female ; Follow-Up Studies ; Gastrointestinal Neoplasms ; metabolism ; pathology ; surgery ; Humans ; Ki-67 Antigen ; metabolism ; Lymphatic Metastasis ; Male ; Middle Aged ; Prognosis ; Synapsins ; metabolism

Objective To investigate the effect of mitochondrial calcium uniporter(MCU)regulator 1(MCUR1)on proliferation,cell cycle and apoptosis of K562 cells and the possible molecular mechanism.Methods Recombinant plasmid vectors containing short hairpin RNAs(shRNAs)targeting MCUR1 were transfected into K562 cells,before the K562 cells stably expressing low MCUR1 were selected with G418.The expression of MCUR1 mRNA was detected by quantitative real-time polymerase chain reaction(qRT-PCR)assays.Western blotting(WB)assays were used to detect the expressions of MCUR1,P53,BAX and BCL2.The proliferation,cell cycle and apoptosis of K562 cells were detected by cell counting kit-8(CCK-8)assays and flow cytometry, respectively.Results The results of qRT-PCR and WB assays revealed that MCUR1 was stably down-regulated at mRNA and protein levels in the K562 cells transfected with shRNAs targeting MCUR1.Knockdown of MCUR1 significantly inhibited the cell proliferation, induced the cell apoptosis, but did not influence the cells cycle.Meanwhile, knockdown of MCUR1 increased the expression of P53 protein and the ratio of protein BAX/BCL2 in K562 cells.Conclusion MCUR1 promotes cell proliferation and inhibits cell apoptosis in K 562 cells.

Adult ; Aged ; Aged, 80 and over ; Aneurysm, Dissecting ; surgery ; Aortic Aneurysm, Thoracic ; surgery ; Endovascular Procedures ; methods ; Female ; Humans ; Male ; Middle Aged

OBJECTIVETo obtain the monoclonal antibody against hexon protein of human adenovirus.

METHODSBALB/c mice were immunized with purified recombinant hexon protein, and the spleen cells of the mice were isolated and fused with myloma cells. Four hybridoma cell strains were screened by indirect ELISA and cultured, and the sensitivity, specificity and virus neutralizing activity were analyzed with ELISA, Western blotting and neutralizing test.

RESULTSThe mouse ascites produced by these hybridoma cells contained specific monoclonal antibodies against hexon protein of human adenovirus as identified by ELISA and Western blot, and the antibody generated by 4C6 strain showed human adenovirus type 3-neutralizing activity.

CONCLUSIONThe monoclonal antibodies against hexon protein with high specificity have been successfully obtained, and these antibodies can be useful in developing assays for early diagnosis of HAdV3 infection and also in study of therapeutic drugs of the infection.

Adenoviruses, Human ; chemistry ; immunology ; Animals ; Antibodies, Monoclonal ; biosynthesis ; immunology ; Antibodies, Viral ; biosynthesis ; immunology ; Blotting, Western ; Capsid Proteins ; biosynthesis ; genetics ; immunology ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; genetics ; Humans ; Hybridomas ; secretion ; Mice ; Mice, Inbred BALB C ; Recombinant Proteins ; biosynthesis ; immunology