Objective To explore the clinical characteristics and treatment of infants with cytomegalovirus(CMV)pneumonia.Methods The clinical and auxiliary examination data of 24 patients with CMV pneumonia,which were diagnosed by detection of serum CMV-IgM used enzyme immunodot technique.All patients were treated with ganciclovir (GCV).Results The most common clinical manifestations of CMV pneumonia were cough,breathlessness,fever,crackles and wheezing rale.Chest X-ray findings predominately revealed increased and thickened bilateral lung markings and patchy areas of increased opacity in both lungs.Sixteen cases were cured,8 cases improved.Conclusions Clinical manifestations of infant CMV pneumonia lack specificity.The presence of serum CMV-IgM antibody is a laboratory diagnostic evidence.GCV is the best choice of drug in treatment of infants with CMV pneumonia.

OBJECTIVE: To observe rule of medicine concentration of blood and the last concentration that through hemoperfusion after poisoned by acephate. METHODS: Utilizeng the patient annual bonus venous blood in hospital emergency room, the content of acephate in plasma was analyzed by gas chromatography. RESULTS: After hemoperfusion, the concentration of acephate showed a rapid drop and the characteristic that the concentration drops quicker if medicine concentration of blood before hemoperfusion is higher. CONCLUSION Hemoperfusion is able to rapidly reduce the concentration of acephate in blood, its speed is determined by initial concentration and the beginning time of hemoperfusion etc.
Acute Disease ; Adult ; Charcoal/therapeutic use* ; Chromatography, Gas ; Coma/therapy* ; Emergency Service, Hospital ; Female ; Hemoperfusion ; Humans ; Male ; Middle Aged ; Organothiophosphorus Compounds/poisoning* ; Phosphoramides ; Poisoning/therapy* ; Time Factors

Objective To explore psychological stress factors of patients in the ophthalmology department and psychological nursing interventions as well as effect, to promote the rehabilitation of patients. Methods A total of 289 patients from our ophthalmology department was investigated with the self-designed questionnaire. Results Major psychological stress factors of Ophthalmology inpatient were knowing nothing a variety of points for attention(92％), fearing and worrying the disease and surgery(85％), worrying about the level of health care technology of medical staff (84％). Psychological stress of patients released through psychological nursing intervention measures such as cognitive intervention and environmental intervention. 243 patients of fearing and worrying the disease and surgery reduced to 35 before and after intervene, The difference was statistically significant (X2 = 299.84, P ＜ 0. 01). Conclusions We should properly guide and inspire positive mental patients based on patients' mental state, give mental nursing intervention, promote the rehabilitation of eye disease.

Adolescent ; Adult ; Buttocks ; injuries ; surgery ; Child ; Child, Preschool ; Contracture ; surgery ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Objective To investigate the association of cytochrome P450 2C9 (CYP2C9 * 3) (1075A ＞ C) gene polymorphisms with the susceptibility to epilepsy and the serum concentration of valproate acid (VPA).Methods DNA was extracted from peripheral blood of 245 healthy subjects and 191 patients with epilepsy.The CYP2C9 * 3 genotype was detected by sequenom mass array method.The steady-state serum concentration of VPA was determined by fluorescence polarization immunoassay.Results The frequencies of CYP2C9 * 3 alleles in patients with epilepsy and healthy subjects were 4.45％ and 2.04％,respectively,and the difference was statistically significant (P ＜ 0.05).The C allele frequency odds ratio was 2.23,C allele increased the risk of epilepsy (P ＜0.05).The steady-state VPA concentration in patients with CYP2C9 * 1/* 1 genotype and patients with CYP2C9 * 1/* 3 genotype were (55.90±21.11) and (67.75 ±21.36)μg· mL-1,respectively,and the difference was statistically significant (P ＜ 0.05).Conclusion CYP2C9 * 3 significantly increases the risk to develop epilepsy and C allele is a susceptible allele for epilepsy.CYP2C9 * 3 polymorphisms are associated with serum concentrations of VPA in epilepsy patients.

Objective: The current study was designed to explore the relationship between the epithelial cadherin (E-cd) expression and clinical appearances of salivary tumor. Method: The paraffin embedded tissue specimens were studied immunohistochemically using the ployclone antibody against E-cd and ABC method. The E-cd expression level was determined semiquantatively. The semi-quantitative parameters of E-cd expression were analyzed retrospectively with the clinical information. Result: The E-cd positive expressions were normal (＋ ＋ ) in two cases of normal salivary tissue, 5 cases of benign mixed tumor and paracancerous salivary tissue, 39 cases of of 47 malignant salivary malignancies it was decreased even to no E-cd expression. There was a significant correlation between decreased E-cd expression and preoperative clinical appearances, postoperative survival, recurrence or metastasis. Conclusion: The E-cd expression level may be a useful prognostic parameter for salivary malignancies.

AIMTo construct the RNAi eukaryotic vector of inhibitory member of the prohibitin (PHB-1) gene and observe the interfering effect in HEK293 cell line after the vector transfection.

METHODSThe specific Mi RNA sequence was designed according to the PHB-1 sequence in GenBank, complementary single-strand DNA oligonucleotides were designed and synthesized, and annealed the single-stranded oligonucleotides to generate a double strands oligonucleotides , cloned the oligonucleotides into pcDNATM6.2-GW/EmGFP-MiR-PHB to obtain an entry clone and then sequence analysis was performed. The recombinant plasmid was transfected into HEK293 cell by liposome. PHB-1 expression was detected by Western blotting.

RESULTSThe DNA sequence of interest clone to the vector was constructed to generate an entry clone and an expression clone successfully, which were proved by sequence determination. Western blotting analysis demonstrated that PHB-1 MiR RNA expression construction could suppress the expression of PHB-1.

CONCLUSIONA RNAi eukaryotic vector containing prohibitin gene was successfully constructed.

Genetic Vectors ; genetics ; HEK293 Cells ; Humans ; MicroRNAs ; biosynthesis ; genetics ; RNA Interference ; RNA, Small Interfering ; genetics ; Recombinant Proteins ; biosynthesis ; genetics ; Repressor Proteins ; biosynthesis ; genetics ; Transfection

OBJECTIVESTo estimate the clinical safety, efficacy of Epristeride in the treatment of benign prostatic hyperplasia (BPH).

METHODSOne hundred and fourty-one BPH patients, aged from 50 to 83 years old [(66.18 +/- 6.41) yr], were given Epristeride tablets 5 mg p.o., twice daily for 120 days. To synthetically estimate the efficacy by the changing rate of international prostate symptom score (IPSS), maximum flow rate (Qmax), the volume of prostate (V) and the residual urine (Ru).

RESULTSThe total efficacy was 81.29%. The adverse reactions include 1 case (0.72%) of exanthem, 1 case (0.72%) of nausea and vomiting, 1 case (0.72%) of insomnia, 1 case (0.72%) of tinnitus and 3 cases (2.16%) of erectile dysfunction. Two patients discontinued the treatment due to the side effects (1.44%). There was no clinical significantly abnormal laboratory index during the treatment.

CONCLUSIONSEpristeride tablets was safe and effective in the treatment of BPH.

Aged ; Aged, 80 and over ; Androstadienes ; adverse effects ; therapeutic use ; Humans ; Male ; Middle Aged ; Prostatic Hyperplasia ; drug therapy ; Treatment Outcome

Animals ; Electrocoagulation ; Microwaves ; therapeutic use ; Pneumococcal Infections ; metabolism ; Proliferating Cell Nuclear Antigen ; metabolism ; Rabbits ; Spleen ; metabolism ; Splenectomy ; methods

OBJECTIVETo research on the main pattern of hepatic cells death during hepatic ischemia/ reperfusion (I/R) injury in cirrhotic rat.

METHODSCirrhotic rat model was established by carbon tetrachloride replication. These rats were randomly divided into sham operation group and I/R group. In the I/R group, 70% i/R injury model was established and then the liver samples were taken 0, 1, 6, 24, and 48 hours after reperfusion. Serum alanine aminotransferase (ALT)/aspartate aminotransferase (AST) levels, Na+ - K+ ATPase, and Ca2+ ATPase were compared. the percentage of apoptotic/oncotic hepatic cells was measured with flow cytometry, and the changes in hepatic cellular structures were observed under transmission electron microscope.

RESULTSCompared with the sham operation group, the levels of serum AST and ALT significantly increased in the I/R group (P < 0.05), reaching their peak levels at the 6th hour. The activities of Na+ - K+ ATPase and Ca2+ ATPase dramatically decreased one hour after reperfusion and then gradually recovered (P < 0.05). Hepatic cells mainly suffered oncosis at the early stage after reperfusion (within 6 hours); at the late stage (around 24 hours after reperfusion), apoptosis became the main death pattern.

CONCLUSIONOncosis is the main pattern of hepatic cells death during I/R injury in cirrhotic rat, and the severity of hepatic injury correlates with the oncosis.

Alanine Transaminase ; blood ; Animals ; Apoptosis ; Aspartate Aminotransferases ; blood ; Disease Models, Animal ; Humans ; Liver Cirrhosis ; blood ; physiopathology ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Reperfusion Injury ; blood ; physiopathology