Objective To investigate the relationship between CRP and CK-MB among acute and stable patients with COPD in Nanjing city. Methods Using case-control design, 81 COPD patients and 71 normal controls were selected. Both fasting venous and arterial blood samples were collected for COPD patients at the acute and the stable stage separately, while fasting venous blood samples were collected for controls during medical examination. The concentrations of CRP, CK-MB or PaO2 of all blood samples were examined. Results The concentration of CRP and CK-MB were significantly higher among stable COPD patients (7.18?5.62, 10.92?5.33; respectively) than those among controls(3.00?0.91, 3.11?1.46; respectively), while acute patients (51.22?24.53,30.06?16.68; respectively) got much higher concentration of CRP and CK-MB than stable patients did. However, PaO2 was significantly higher among stable COPD patients than that among acute patients. For acute COPD patients, the concentration of CK-MB positively correlated with CRP, while PaO2 negatively correlated with CRP and CK-MB separately. Conclusions CRP and CK-MB were sensitive predictors of COPD status to the transition from stable to acute stage of COPD, and both negatively correlated with PaO2 among these sample COPD patients.

Objective To study the interaction of tetramethylpyrazine and human serum albumin(HSA).Methods The fluorescent quenching of HSA with te tramethylpyrazine has been detecte d by fluorimetry.Results It was found that fluo-rescence of HSA were quenched by tetr amethylpyrazine.The fluorescent q uenching data were analyzed accordi ng to Stern -volmer equation and the bindi ng constant was obtained.Conclusion The mechanism of fluorescent quench ing is considered to be the formation of HSA-tetramethylpyrazine complex.

Objective To explore the changes of intereferon-?(IFN-?)and interleukin-4(IL-4)in peripheral blood of children with mycoplasma pneumoniae(MP) encephalitis at the acute phase.Methods The peripheral blood concentrations of IFN-? and IL-4 were measured by enzyme-linked immunosorbent assay(ELISA) in 24 cases of children with MP encephalitis at the acute stage.The samples from 24 cases of healthy children were control group.Twenty-four children with MP encephalitis were intravenous drip with azithromycin,at the same time,10 cases received hexadecadrol and 15 cases received gamma globulin.Results The serum concentrations of IFN-? and IL-4 in the mycoplasmal encephalitis group were(98.56?12.93) and(45.55?17.58) ng/L,respectively,which were significantly higher than those in control group [(85.35?6.91) and(26.78?9.89) ng/L] respectively(Pa

ObjectiveTo compare the outcomes of total hip replacement (THR) and minimally invasive dynamic hip screw (MIDHS) in treating osteoporotic femoral intertrochanteric fractures so as to discuss the differences of the two treatment methods.Methods A retrospective study was done on 48 elderly patients with femoral intertrochanteric fractures.All the patients were randomly treated with THR (THR group) and MIDHS internal fixation ( MIDHS group).After a follow-up of over six months,the two groups were compared concerning the operation time,intraoperative blood loss,postoperative drainage,postoperative hip function,and postoperative complications.ResultsTHR group had longer operation time,larger volume of intraoperative bleeding and postoperative drainage as compared with the MIDHS group.The incidence of internal fixation loosening varied significantly with different degree of osteoporosis in the MIDHS group,but not in the THR group.The loosening rate of the implants in the MIDHS and THR groups was 33.3％ and 4.1％ respectively,with significant difference.Functional evaluation of hip showed no significant differences between the two groups six months postoperatively.ConclusionFor achieving earlier and better functional recovery and less postoperative complications in the management of osteoporotic femoral intertrochanteric fractures,MIDHS can be considerel for the elderly patients with low degree of osteoporosis and THR for younger patients with high degree of osteoporosis.

ObjectiveTo discuss the role of transient receptor potential vanilloid 1 (TRPV 1) in the regulation effect of electroacupuncture on gastric motility.MethodTRPV1 gene knock-out mice (KO mice) and wild-type C57BL/6 mice (WT mice) were selected to receive acupuncture at Zusanli (ST36),Quchi (LI11), Zhongwan (CV12), and Weishu (BL21), and the intragastric pressure was observed before and after acupuncture.ResultElectroacupuncture at Zusanli caused both excitation and inhibition in WT mice, predominated by mild excitation, while electroacupuncture at Weishu, Quchi and Zhongwan all caused inhibition effect; in the KO mice, electroacupuncture at Zusanli, Quchi, Zhongwan, and Weishu all inhibited gastric motility.Conclusion TRPV1 bears certain regulating effect on gastric motility, andacupuncture can inhibit the gastric motility in TRPV gene KO mice.

The clinical data of 42 patients with pelvic organ prolapse, who underwent laparoscopic sacral colpopexy using prolene meshes were retrospectively reviewed. During the operation prolene meshes were placed in the rectovaginal septum and vesicovaginal septum ,and the uterus was preserved in all cases.The mean operation time was (92 ± 12)min and the mean blood loss during the operation was (98 ± 11 )ml.Postoperatively, both prolapse and symptoms were significantly improved ( P ＜0. 01 ) according to the pelvic organ prolapse quantification (POP-Q) system. The operation is an effective and minimally invasive new technique which offers a chance for patients with pelvic organ prolaps who desire to preserve uterus.

Objective To investigate the protective effects of propofol against acute lung injury(ALI) induced by oleic acid.Methods Forty adult male SD rats weighing 250-290 g were anesthetized with intraperitoneal(i.p.)20% urethrane 6 ml?kg~(-1) and tracheostomized.Left common carotid artery and right internal jugular vein were cannulated for BP monitoring and fluid and drug administration.The animals were randomly divided into 5 groups (n=8 each):Ⅰ control group;Ⅱ ALI group in which ALI was induced by oleic acid 250 mg?kg~(-1) i.v.;Ⅲ,Ⅳ and Ⅴ ALI+propofol group in which propofol was continuously infused i.v.at 4, 8 and 16 mg?kg~(-1)?h~(-1) for 4 h immediately after i.v.oleic acid.The animals were killed at 4 h after oleic acid administration.The lungs were immediately removed for(1)examination of ultrastructure of the lung with transmission electron microseope and(2)determination of SOD and MPO activity,content of MDA,level of IL-10 and IL-18 and expression of NF-kB in lung tissue.Results In group Ⅱ intravenous oleic acid produced damage to mitochondria,rough endoplasmic reticulum and osmiophilic multi-lamellar body in type Ⅱ alveolar epithelial cells. Propofol infusion in group Ⅲ,Ⅳ and Ⅴ attenuated the damage to different degrees.In group Ⅱi.v.oleic acid produced significant decrease in MPO and SOD activity and significant increase in MDA content,IL-10,IL-18 and NF-kB expression in lung tissue.Intravenous propofol infusion attenuated the decrease in MPO and SOD activity, increase in IL-18 expression and MDA content and NF-kB expression in lung tissue produced by i.v.oleic acid, but increased IL-10 expression in lung tissue further.The best protective effect was seen in group Ⅳ.Conclusion Propofol i.v.infusion at 4-16 mg?kg~(-1),h~(-1) can inhibit the oxidative response and inflammatory response and down-regulate NF-kB expression in lung tissue.Propofol infusion at 8 mg?kg~(-1)?h~(-1) provides best protective effects.

Objective:To investigate the clinical incidence of the three mesial root canals of mandibular first molars. Methods: Incidence and morphology of the three mesial root canals of mandibular first molars were determined by probing the fissure or grooves between the mesiobuccal and mesiolingual root canal orifices and X-ray analysis. Results: Of the 222 mandibular first molars, 13 of the molars had three mesial root canals. The incidence rate was 5.85 %. Conclusion: Although the incidence rate of the three mesial root canals of mandibular first molars was very low, the dental operative microscope was helpful for the diagnosis.

Objective:To investigate the HLA-DRB1 allele polymorphism of the Tu race population in Qinghai region of China.Methods:Polymerase chain reaction and hybridizations of sequence-specific olignucleotide probe was used to detect HLA-DRB1 alleles in 50 unrelated healthy Tu individuals from Qinghai region,and the results were compared with those of minority populations in China.Results:Sixteen of alleles were detected and identified for HLA-DRB.HLA-DRB11*04,DRB1*08,DRB1*14,DRB1*15,DRB3*,DRB4* were the most common alleles.The frequency of DRB1*06,DRB1*07,DRB1*09,DRB1*13,DRB1*16,DRB1*23 were lower.Conclusion:The allelic polymorphism of HLA loci of Tu race population in Qinghai area has its own characteristics.

Objective: To investigate the hyponatremia incidence in patients with ruptured intracranial aneurysms and the correlation with Fisher grading and cerebral vasospasm. Methods: Totally 150 patients with ruptured intracranial aneurysms from March 1998 to May 2001 were divided into anterior communicating artery (ACoA) group,posterior communicating artery (PCoA) group and middle carotid artery(MCA) group according to the sites of aneurysms.The patients were treated with GDCs interventionally and the incidences of hyponatremia were analyzed. Results: The incidences of hyponatremia in 3 groups were 48%(32/67),15%(8/52) and 16%(5/31),respectively; The incidences of severe hyponatremia of 3 groups were 30%(20/67),6%(3/52) and 6%(2/31), respectively. In ACoA group, the incidences of hyponatremia in Fisher grade Ⅲ-Ⅳ and Ⅰ-Ⅱ were 67%(20/30) and 32%(12/37) and the incidences with and without vasospasm were 71%(24/34) and 24%(8/33), respectively. Conclusion: The ACoA group has a significantly higher incidence of common and severe hyponatremia than the other groups. In ACoA group,hyponatremia is significantly more often in Fisher grade Ⅲ-Ⅳ cases and those with vasospasm.