Objective:To test the microtensile bonding strength between root canal wall and Bis-Core, and that between root canal wall and Chemil Superior glass-ionomer.Methods:10 human maxillary incisors extracted within one week were kept for another one week after root canal treatment and removeal of the crowns,each root was splited into two parts along with the long axis by Isomet saw, the bonding interface on one third of upside of root dentin smear layer was made with Diamond bur at a high-speed. Then Bis-Core and Chemil Superior glass-ionomer were put on root canal inner surface of each half part of the root respectively, samples with the size of 1.00 mm?1.00 mm were prepared by Isomet saw. Two samples in which bonding interface was perpendiculur to load were selected and fixed in Microtensile testing machine for bonding strength test. Results:Microtensile bonding strength(MPa) between Bis-Core and root canal inner wall and that between root canal inner wall and Chemil Superior glass-ionomer was 20.65?1.72 and 12.89?1.63 respectively(P

Objective:To observe the microstructure of interface between material and dentin after root canal rehabilitation. Methods:Three human maxillary incisors extracted within one week were used. After crowns being removed and received root canal treatment, they were used for the study. The root was split into two parts along the long axis, then the bonding interface was made with high speed diamond bur. Bis-Core and Chemil Superior glass-ionomer were stacked on root canal inner wall respectively and the teeth were split along with the long axis with Isomet saw, in order to expose bonding interface. The microstructure of bonding interface of the samples were analyzed by optical microscope and atomic force microscope(AFM). Results: AFM observation of the adhesive interface specimens showed that Chemil Superior glass-ionomer and teeth knitted together completely and no filling invaded into dentin tube. While the bonding interface between Bis-Core and dentin, dentin primer infiltrated into dentin tube and matrix around through the smear layer and colophony protruded. Conclusion:The observations reveal the extent of resin penetration into the dentinal tubules provides a very strong bonding. This is a fundamental cause of resin better bond to tooth.

Objective:To investigate the infiltration of CD45RO+ T cells and CD68+ cells in the lesions of non-neoplastic epithelial disorder and in vulva cancer.Methods:The infiltration of CD45RO+ T cells and CD68+ cells was detected with S-P immunohistochemistry in the lesions of 20 non-neoplastic epithelial disorders,including vulvar squamous cell hyperplasia(SH) and vulvar lichen sclerosus(LS)) and vulvar cancer,respectively.The vulvar skins from 10 healthy individuals were selected as the control.Results:The infiltration of CD45RO+ T cells and CD68+ cells in healthy vulvar skin was very low and significantly lower than that in SH,LS and vulvar cancer(P0.05).The number of both cells were significantly higher than that in SH,poorly vulvar cancer and early stage of LS(P0.05).The infiltration of CD68+ cells was shown in a positive correlation with CD45RO+ T cells in the different lesion of vulva,the correlation coefficient was 0.742(P

OBJECTIVE:To investigate the distribution of magnetic nanoparticle embedding Paclitaxel in vivo and its curative effect on lung cancer in mice model.METHODS:The magnetic lipsomes containing Paclitaxel and the Paclitaxel liposomes were injected into caudal vein of mice,and which were exposed to magnetic field of 0.5T.The concentrations of the two drugs in different organs were determined.The lung cancer model was established in mice and the therapeutic efficacy of the different drugs on tumor was evaluated by comparing the weight of mice before and after administration.RESULTS: In the mice treated with magnetic liposome containing Paclitaxel,the drug concentration in lung which was exposed to magnetic field was significantly higher than that in the others sites,and a steady state concentration was reached quickly in lung after administration,but gradually in other organs.In the mice treated with liposomes containing paclitaxel,a steady state concentration was reached gradually in lung.The tumor control rate was 27.53% in mice treated with magnetic liposome containing Paclitaxel plus magnetic field intervention,significantly higher than in the control group.CONCLUSION: The result shows that magnetic targeting drug delivery is an effective drug delivery system,by which,the drugs can aggregate to the target site in large quantity which may lead to a reduced distribution of drugs in non-target sites.

Objective For arising recognition to HIE of neonate.We made a summary and a retrospective studied to CT material of 130 cases for neonate suffering HIE,combined with clinical diagnosis and documents.Methods 130 cases for neonate suffering HIE diagnosed by CT and clinic,all underwent unenhanced CT scan.Results Appearance of HIE:Normal involved 1～4 brain′s lobe,often involved frontal lobe;can be accompanied by kinds of intracranial hemorrhage;can be accompanied by infarction.Conclusion Among HIE cases,hypoxia and ischiemia were cause and result each other;difference among clinical symptoms and CT apearances are possible;the neonate with moderate and sereve asphyxia can be accompanied by intracranial hemorrhage more easier;to the injury of parasagittal region,CT and USG can not make a diagnosis.

Reviews research findings about aberrant crypt foci on concepts, relation to colon carcinogenesis, relative genes and biomarkers, methods, signification. It shows that aberrant crypt foci are possible precursors of adenoma and cancer, which can be early diagnosis marks of colon carcinogenesis.

Objective To observe the therapeutic effect of hyper-laser irradiation combined with acupuncture for the treatment of temporomandibular joint disturbance syndrome(TMJDS).Methods Sixty TMJDS patients were equally randomized into two groups: the treatment group was given hyper-laser irradiation combined with acupuncture,and the control group given acupuncture alone.The treatment lasted 10 days.After treatment,the improvement of symptoms and signs in both groups was evaluated.Results The total effective rate was 93.3% in the treatment group and 80.0% in the control group,the therapeutic effect in the treatment group being superior to that in the control group(P

[Objective] To explore the treatment of urinary incontinence after stroke.[Methods]To use the Acupucture and Moxibustion method to treat the 35 patients who met the inclusion criteria,compared before and after treatment of urinary incontinence in patients with the degree of improvement.[Results] After two courses of treatment,the total effective rate 94.286%.[Conclusion]Acupuncture and Moxibustion treatment of urinary incontinence after stroke have a positive effect,and this method is simple and few side effects.

Objective To investigate the inter-arm invasive blood pressure differences during hip replacement in patients with lateral position and its relationship with position.Methods Sixty-six patients having underwent hip replacement in orthopedic surgery with lateral position were enrolled.Invasive blood pressure measurements were obtained from bilateral radial artery in all patients every 5 minutes.Simultaneously bilateral invasive blood pressure measurements were obtained and compared.Results At supine position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (128.16 ±20.09),(69.47 ± 8.64) and (89.03 ± 10.42) mmHg (1 mmHg =0.133 kPa);systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (127.90 ± 20.44),(69.86 ± 8.81) and (89.21 ± 10.71) mmHg.There was statistical difference in inter-arm diastolic pressure (P ＜ 0.05),but inter-arm systolic pressure and mean arterial pressure had no statistical differences (P ＞ 0.05).At left-lateral position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (129.12 ± 21.93),(69.23 ± 8.92) and (89.19 ± 11.35) mmHg;systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (129.01 ± 21.72),(69.51 ± 9.02) and (89.34 ± 11.35) mmHg.There was statistical difference in inter-arm diastolic pressure (P ＜ 0.05),but inter-arm systolic pressure and mean arterial pressure had no statistical differences (P ＞ 0.05).At right-lateral position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (128.78 ± 20.34),(69.54 ± 9.06) and (89.29 ± 10.82) mmHg;systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (128.27 ± 20.56),(70.00 ± 9.09) and (89.43 ± 10.89) mmHg.There were statistical differences in inter-arm systolic pressure and diastolic pressure (P ＜ 0.05),but inter-arm mean arterial pressure had no statistical difference (P ＞ 0.05).The bilateral radial artery blood pressure difference under different positions was ＜ 1 mmHg,and had no clinical practical significance.There was no correlation between blood pressure and age,sex,body mass index.Conclusion Bilateral radial artery in the same patients with invasive blood pressure has no clinical difference,and patients with lateral position operation for radial artery puncture pressure need not consider the differences of blood pressure between arms.

BACKGROUND:The increase of serum cholesterol levels is positively correlated with the increasing incidence of osteoarthritis. Little is known about the role of high-fat diet in the degeneration of articular cartilage and induced arthritis.
OBJECTIVE:To observe the morphology change of the articular cartilage of New Zealand rabbits after high-fat diet, and explore the role of food in the articular cartilage degeneration.
METHODS:Forty New Zealand white rabbits were randomly divided into two groups. In control group, rabbits were fed with basal diet. In high-fat diet group, rabbits were fed with high-fat diet (20%lard and 80%basal diet). Fasting blood samples was taken every 4 weeks, to detect the triglyceride and total cholesterol levels. After rabbit were fed for 28 weeks, the knee joint was grossly observed and the femoral condyle cartilage was scanned by scanning electron microscope.
RESULTS AND CONCLUSION:Compared with the control group, the triglyceride and total cholesterol levels were significantly increased in the high fat group (P<0.05). Under scanning electron microscope, the high-fat group showed rough surface of condylar cartilage, shal ow pouch, messy arrangement, irregular shape and uneven size. The surface of cartilage exhibited focal denudation, irregular arrangement, uneven porosity and fractured cavity. Under higher magnification, smal mountain-like structure of cartilage surface became shal ow and flattened, the pores disappeared. Long-term high-fat diet may induce and aggravate cartilage damage, suggesting it may be involved in the pathogenesis of osteoarthritis.