Acute Disease ; Adult ; Aged ; Asthma ; chemically induced ; Chlorine ; poisoning ; Humans ; Male

Objective To quantitatively assess cardiac intervals with tissue Doppler imaging(TDI) and normal reference values. Methods Apical 4 chamber, 2 chamber and long axis views of two-dimensional TDI were acquired in 29 healthy individuals(using GE Vivid 7 commercially ultrasound machine). The basal and middle segments in left ventricle(anterior septal, anterior, lateral, posterior, inferior and septal wall) and in the free wall of right ventricle were analyzed with tissue velocity imaging(TVI) model.Cardiac intervals of each segment, which included the isovolumic contraction time, contraction to peak time, insovolumetric relaxation time, rapid filling time and artrial contraction time were measured using TVI in the left ventricle and free wall of the right ventricle. Results The successful rate of sample was 100% in basal segment of each wall. There were significant differences between the left and right ventricle in the isovolumetric contraction time, the systolic accelaration time, contraction to peak time, isovolumetric relaxation time, early diastolic time, diastasis and artrial contraction( P

The development of the endocardial cushion and the ventricular septum of chicken heart was studied with SEM, TEM, light microscopy and histochemical methods. The results are as follows:1. The development of endocardial cushion begins from the proliferation of the endothelial cells which form cushion cells when these cells enter the cardiac jelly. Cushion cells also proceed mitosis and migrate toward the myocardium, and the endothelial cells cease proliferation when the reserve layers of cushion cells were formed. The fused endocardial cushion participates in the formation of atrioventricular orifice, the right tubercle prolongs toward the ventricular septum and eventually fuses with it.2. The cardiac jelly of endocardial cushion contains a great deal of hyaluronic acid which arranges in "trails", pioneering cushion cells migrate along the "trails" and change the component and arrangement of the matrix.3. Muscular ventricular septum is formed by aggregation of trabeculae inside the interventricular sulcus. The dorsal part of the ventricular septum fuses with the lower part of right tubercle of endocardial cushion and the ventral part fuses with the conus septum.

The morphogenesis of the heart and the formation of the epicardium were studied with SEM, TEM and light microscopy. The results are as follows:1. There are two stages in the development of chicken heart: First is the formation of cardiac loop. In certain places, the sulcus appears outside, and the cresta appears inside during this stage. The formation of the cardiac loop indicates the establishment of the anlagen of the heart; Second is the differentiation of the anlagen, including the septation of the heart and the shift of each part of the heart. The shift of the atrium and the conus results from the shift of the right and left ventriculum. 2. The epicardium is not differcntiatcd from the myocardium, but formed from the mesenchymal cells which proliferate in front of the sinus venosus. These cells migrate and cover the entire surface of the myocardium.

Objective Treatment remnant electrode infection of permanent cardiac pace makers Methods Remnant electrodes were wrapped in silicone adhesive and covered with skin Results In 16 patients of permanent pace makers with infections of remnant electrodes, by using the method of wrapped and covered electrodes, infection did not occur in the observation of 3 to 24 months Conclusion It is an effective method to treat the infection caused by remnant electrodes of pace makers by means of silicone adhesive wrapping and covering electrodes

Objective To explore the reparative effect of bactericidal/permeability-increasing protein(BPI) on the damaged mucosa of rats with otitis media with effusion (OME),and state the pathogenesis of OME.Methods Wistar rats(40 ears) were randomly divided into six groups:normal control group (n=4),BPI control group(n=4),eustachian tube obstruction (ETO) group (n=8),lipopolysaccharide (LPS) injection group (n=8),ETO+LPS group (n=8),ETO+LPS+BPI group (n=8).The experimental OME model was made through eustachian tube obstruction and LPS injection.The rats were killed after 1,2 and 4 weeks and the changes of mucosa of middle ear were observed under light and scanning electron microscope.Results The rats in normal control group and BPI control group had the normal mucosa in the tympanic orifice of the eustachian tube.It consisted of pseudostratified ciliated cubical or columnar epithelium which contained an abundant number of ciliated cells and a few goblet cells,these were the mucociliary clearance system of the middle ear.The hypotympanum consisted of thin,squamous epithelium with few microvillus.Middle ear mucosa was obviouly thickened in LPS injection,ETO and ETO+LPS groups.An increase in goblet cells and a decrease in ciliated cells were observed in the tympanic orifice of the eustachian tube.The epithelial layer in the hypotympanum had become more pseudostratified ciliated cubical epithelium.In ETO+LPS+BPI group,there was thin squamous epithelium in the hypotympanum near normal,which was not thickened and contained few microvillus. Conclusion LPS and ETO can result in the occurrence and protracted courses of OME by mimosa's inflammatory reaction which can reduce the activity of ciliary cells and weaken the function of mucociliary clearance system.BPI could bind avidly to LPS,reduce inflammatory reaction,and break the inflammatory cycle and reestablish an effective mucocillary clearance system.The results suggest that BPI treatment is a potential effective drug for prevention and therapy of chronic OME.

Objective To study the mechanism of activity change of gastric alcohol dehydrogenases(ADH).We investigated ADH before and after ingestion of alcohol on rats.Methods Thirty-nine rats were divided into two groups at random:model ones(24 cases) and control ones(15 cases).Model group of rats were infused into stomaches by alcohol,and control ones by distill water.Structures of cell and sub-cell in gastric mucous membrane were observed by enzyme electron microscope and hematoxylin eosin(HE) stain.Results It was shown that there were dropsical degeneration of chief cells and parietal cells of stomaches by HE stain,and the mitochondria of parietal cells and the rough-surfaced endoplasmic reticulum(rER) of chief cells were destroyed by enzyme electron microscope in model groups after two months of ethanol perfusion.There were not change in control group.Conclusions The mechanism of activity alterations of gastric ADH may result from dropsical degeneration of chief cells and parietal cells of stomach,which result in degeneration,solution and breakage of construction of rER in the chief cells and mitochondria in parietal cells.

Heart Septal Defects, Atrial ; complications ; Humans ; Infant, Newborn ; Male ; Nasal Polyps ; complications ; congenital ; Nasopharyngeal Diseases ; complications ; congenital

Eukaryotic elongation factor 2 kinase (eEF2K) is well known as a Ca2+/calmodulin (CaM)-dependent kinase. eEF2K catalyzes the phosphorylation of eEF2 and subsequently inactivates eEF2 by impairing its ability to bind to the ribosome, thereby negatively modulates protein synthesis. The high expression of eEF2K has been found recently in several types of malignancies. As participating in the progress of tumor, eEF2K emerges a potential target for future cancer therapy. The relationship between eEF2K and tumor, and the latest progress of eEF2K inhibitors were summarized in this article.
Elongation Factor 2 Kinase ; antagonists & inhibitors ; metabolism ; Humans ; Neoplasms ; metabolism ; Peptide Elongation Factor 2 ; metabolism ; Phosphorylation

Analysis and summarize the causes and principles of the cases of tracheal tube rupture. Report one case of adult tracheal tube rupture. Review the past literature content and combine with our experience. Comprehend the clinical manifestations and treatment of tracheal tube rupture is very important for prevention.
Adult ; Humans ; Intubation, Intratracheal ; Rupture ; etiology ; prevention & control ; Trachea ; injuries