1.Egr-1 induces osteogenic differentiation of BMSCs by promoting NDRG1
Suqin SHI ; Yan PAN ; Xin YUE ; Yan CHEN ; Lu ZHAO
Chongqing Medicine 2017;46(4):442-445
Objective To explore the effects of early growth response gene-1 (Egr-1) on bone marrow mesenchymal stem cells (BMSC) proliferation and osteogenic differentiation,which is aimed at providing new molecular targets for the treatment of osteoporosis.Methods Bone marrow was collected from adult men and the BMSCs were cultured primarily and observed by microscope.Meanwhile,flow cytometry was used for BMSCs phenotypic identification;After transfection of pcDNA3.1/Egr-1 into BM SCs,the level of BMSCs proliferation was determined by MTT respectively on the 2 d,4 d and 6 d;On the 7 d after transfection,the ALP activity assay was used for testing the ALP activity in BMSCs.And then,alizarin red S-calcium kit was used for measuring the calcified knots respectively on the 7 d,14 d and 21 d;On the 21 d after transfection,real-time qPCR and Western blotting were used respectively for measuring the expression of mRNA and protein of Egr-1,Runx2 and NDRG1;Further,BMSCs were transfected with Egr-1 siRNA,and the content of calcium nodules,ALP activity,the expression of Egr-1,Runx2 and NDRG1 were detected as above methods.Results The cells cultured in vitro showed high level of CD90 and CD29 and very low level of CD34 and CD45,which is accorded with the characteristic of BMSCs.The pcDNA3.1/Egr-1 transfection for BMSCs had no effect on cells prolifera tion.However,the calcified knots,ALP activity and the expression of Egr 1,Runx2 and NDRG1 were increased after transfection of pcDNA3.1/Egr-1 for BMSCs.In addition,Egr-1 siRNA showed the opposite effect with pcDNA3.1/Egr-1 transfection for BMSCs.Conclusion Egr-1 induces osteogenic differentiation of BMSCs by promoting NDRG1 but has no effects on proliferation of BMSCs.
2.Development progress and application of monoclonal antibody drugs against respiratory virus
ZHOU Jian⁃hua ; YUE Xin ; PAN Yong⁃bing
Chinese Journal of Biologicals 2023;36(1):89-97
Abstract:In recent years,the outbreak and prevalence of respiratory infectious diseases in the world seriously endanger
human health,among which the respiratory infectious diseases caused by viral infection account for a large proportion. The
use of vaccines and common antiviral drugs is an effective way to fight viral infection,but there are also problems such as lag
and drug resistance. Monoclonal antibodies against respiratory viral infections provide a new strategy for clinical
treatment. This paper reviews the development of monoclonal antibody against respiratory virus and its application in
respiratory viral infectious diseases.
Keywords:Respiratory viral infectious diseases;Respiratory syncytial virus(RSV);Influenza virus(IFV);Coronavirus
(CoV);Monoclonal antibody
3.Advancement of insulin effecting signaling pathway of leukemia cell proliferation.
Journal of Experimental Hematology 2012;20(2):514-517
Many reports have documented a role of insulin and insulin-like growth factor 1 (IGF-1) as growth factors in many cancers. The sequence and structure of insulin receptor (IR) and IGF receptor (IGF-1R) are highly similar. Both receptors are overexpressed in leukemia cells.Studies indicate that insulin can enhance the signal of the phosphoinositide 3-kinase/Akt pathways by activating IR or IGF-1R or hybrid IR/IGF-IR receptors, resulting in the proliferation of leukemia cells. High concentration of insulin may inhibit the growth of leukemia cells, the mechanism of which remains to be unclear. Inhibiting IR and IGF-IR can diminish the proliferation of leukemia cells. Therefore, the assumption of IR/IGF-1R as a potential therapeutic target in leukemia appears reasonable. This article summarizes the recent advancement associated with the signaling pathway of insulin effecting the proliferation of leukemia cells.
Cell Line, Tumor
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Cell Proliferation
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Humans
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Insulin
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Insulin-Like Growth Factor I
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metabolism
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Leukemia
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metabolism
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pathology
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Receptor, Insulin
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metabolism
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Signal Transduction
4.The effect of proteasome inhibitor on NF-κB signal path in a rat model of knee osteoarthritis
Renfu QUAN ; Zhongming HUANG ; Zhenshuang YUE ; Dawei XIN ; Disheng YANG ; Jie PAN ; Liang ZHANG
Chinese Journal of Orthopaedics 2012;32(6):582-589
Objective To observe the effect of MG-132 on NF-κB signal path of cartilage and synovium in a rat model of knee osteoarthritis.Methods The rat models of knee osteoarthritis were established by performing anterior cruciate ligament amputation and partial medial meniscectomy.Totally 144 adult SD rats were randomly divided into 4 groups:MG-132 group,100 ml 0.007 g/L MG-132 solution was injected in to the knee joints of rat model 24 h after surgery; DMSO group,100 ml 0.1% DMSO solution was injected 24 h after surgery; sham surgery group,merely the knee capsulotomy was performed and no solution was injected;control group,100 ml 0.007 g/L MG-132 solution was injected into the knee joints.The cartilage and synovium specimens were obtained at 2,4,12 weeks postoperatively.Pathomorphological observation was taken.The levels of NF-κB p65,I-κB,TNF-α and IL-1β at mRNA were detected by real-time PCR,and the activityof 20S proteasome was measured by fluorospectrophotometry.Resnlts The Mankin score of MG-132 groupwas lower than that of DMSO group.The Mankin scores of sham surgery and control groups were lower thanthose of MG-132 and DMSO groups with significant difference.The mRNA levels of NF-κB p65,IL-1 β,TNF-α of cartilage and synovium in MG-132 group were lower than those of DMSO group with significant differenceexcept for NF-κB p65 of synovium at 2 weeks and IL-1β of cartilage at 12 weeks.The mRNA levels of I-κB of cartilage at 2 weeks and I-κB of synovium at 4 weeks in MG-132 group were higher than those in DMSO group with statistical significance.Conclusion MG-132,the proteasome inhibitor,could postpone the progress of osteoarthritis through alleviating synovial inflammation and defending the articular cartilage.
5.Exploration on the Research-oriented Teaching Reform of Microbiology
Yue-Lan YIN ; Xin-An JIAO ; Zhi-Ming PAN ; Lin SUN ; Jin-Lin HUANG ; Xiang CHEN ;
Microbiology 2008;0(12):-
This paper is discussed about course system construction of Microbiology, teaching method, in- struction means and experimental teaching mode. Teaching practice indicated that reform the pattern of Mi- crobiology educational mode can stimulate students’ interest in studying the course, cultivate their inde- pendent ability to solve questions, develop their creative thinking. It is an important way to train high-caliber talents.
6.Pharmacokinetics of loganin, ferulic acid and stilbene glucoside in Bushen Tongluo formula in vivo.
Xiang-dan LIU ; Pan HUANG ; Yue-hua LU ; Ming MA ; Ri-bao ZHOU ; Lin-xiang YUAN ; Xin-jun PENG
China Journal of Chinese Materia Medica 2015;40(12):2428-2434
To study the pharmacokinetics characteristic of loganin, ferulic acid and stilbene glucoside in rat plasma after oral administration of Bushen Tongluo formula. The plasma samples were treated by using liquid-liquid extraction technique, the concentrations were determined by HPLC-UV. Johnson spherigel C18 column (4.6 mm x 250 mm, 5 μm) was adopted and eluted with the of mobile phase of methanol-water containing 0.01% glacial acetic acid in a gradient mode, with the flow rate at 1.0 mL x min(-1), column temperature at 30 degrees C and injection volume of 10 μL. According to the findings, loganin was determined at 235 nm, ferulic acid and stilbene glucoside were determined at 320 nm, with the sample size of 10 μL. The pharmacokinetic parameters of loganin, ferulic acid and stilbene glucoside were calculated by DAS 2. 0 software as follows: C(max) was (0.369 ± 0.042), (0.387 ± 0.071), (0.233 ± 0.044) mg x L(-1); t(max) was (0.226 ± 0.022), (0.282 ± 0.031), (0.233 ± 0.044) h; t(½β) was (6.89 ± 0.20), (10.73 ± 0.11), (6.93 ± 0.09) h; AUC(0-∞) was (1.91 ± 0.36), (3.22 ± 0.52), (1.52 ± 0.33) mg x h x L(-1); AUCO(0-t) was (1.62 ± 0.33), (2.58 ± 0.43), (1.30 ± 0.30) mg x h x L(-1); CL was (20.2 ± 4.0), (1.39 ± 0.23), (31.7 ± 6.9) L x h(-1) x kg(-1), respectively. The results showed that after the oral administration with Bushen Tongluo formula, loganin, ferulic acid and stilbene glucoside showed concentration-time curves in conformity with the two compartment model, with a rapid absorption, loganin and stilbene glucoside was excreted at a moderate speed, and ferulic acid was excreted slowly (but with the highest bioavailability). Bushen Tongluo formula can main maintain plasma concentration with three administrations everyday and so is suitable to be made into common oral preparation.
Administration, Oral
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Animals
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Biological Availability
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Coumaric Acids
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administration & dosage
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blood
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pharmacokinetics
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Drugs, Chinese Herbal
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administration & dosage
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analysis
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pharmacokinetics
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Glucosides
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administration & dosage
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blood
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pharmacokinetics
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Iridoids
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administration & dosage
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blood
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
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Stilbenes
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administration & dosage
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blood
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pharmacokinetics
8.Differences in Variation of Human Immunodeficiency Virus Type 1 Sequences from Henan and Shanghai Regions of China
Chun-yu, YIN ; Hong-zhou, LU ; Wei-ming, JIANG ; Maria Pia De PASQUALE ; Yue-kai, HU ; Xiao-zhang, PAN ; Xin-hua, WENG ; Richard T. D'AQUILA ; Yi-Wei, TANG
Virologica Sinica 2007;22(3):212-217
Illegally paid blood donation was a risk factor for HIV acquisition exclusively in Henan and Hubei Provinces of China, and not in Shanghai. Nucleotide sequences in the gag and env genes of HIV-1 were compared between isolates from Henan and Shanghai regions of China to test whether an expected higher degree of a common source of infections from this unique blood donation transmission risk would be evident as decreased variation among Henan isolates in an exploratory cross-sectional analysis. Among 38 isolates studied, 23 of 23 (100%) from Henan and 8 of 15 (54%) from Shanghai were subtype B. In addition, fewer sequence differences were found in gp41 of subtype B isolates from Henan than from Shanghai isolates. Further studies with additional controls are therefore warranted to confirm the role of the degree of a common source of infections in differences in HIV variation across populations.
9.Chemical constituents from Polygonum lapathifolium
Xin TAO ; Duo PAN ; Nan XU ; Xiao-Yue LIU ; Hui-Ru ZHANG
Chinese Traditional Patent Medicine 2018;40(4):866-870
AIM To study the chemical constituents from Polygonum lapathifolium L..METHODS The ethyl acetate fraction of 80% ethanol extract of P.lapathifolium L.was isolated and purified by silica column,Sephadex LH-20 and ODS,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Fifteen compounds were isolated and identified as 2',4'-di-hydroxy-6'-mehtoxy-chaltone (1),2',6'-di-hydroxy-4'-methoxy-chaltone (2),2',3'-di-hydroxy-4',6'-di-methoxy-chaltone (3),kaempferol (4),quercetin (5),quercetin-3-O-α-L-rhamnoside (6),quercetin-3-O-α-L-rhamnoside (7),kaempferol-3-O-α-L-rhamnoside (8),kaempferol-3-O-β-D-glucoside (9),quercetin-3-O-β-D-galactoside (10),quercetin-3-O-β-D-glucoside (11),kaempferol-3-O-β-D-galactoside (12),quercetin-3-O-(2"-O-galloyl)-rhamnoside (13),quercetin-3-O-(2"-O-galloyl)-glucoside (14),quercetin-3-O-[(6"-O-transferuloyl)-β-D-galactoside] (15).CONCLUSION Compound 3-8 are isolated from this plant for the first time.
10.Clinical study of susceptibility-weighted magnetic resonance imaging in lacunar cerebral infarction.
De-qiang ZHAO ; Su-yue PAN ; Jian-hui CHNE ; Jing-xin WANG
Journal of Southern Medical University 2010;30(4):881-883
OBJECTIVETo evaluate the clinical application of susceptibility-weighted magnetic resonance imaging (SWAN) in lacunar cerebral infarction imaging.
METHODSForty-two cases of lacunar cerebral infarction, including 18 complicated by high blood pressure, 4 by type 2 diabetes and 12 by both high blood pressure and type 2 diabetes, underwent examinations with SWAN and conventional MRI sequences (including GRE sequence T(1) and T(2), T(2) gradient echo, T(2) FLAIR, DWI). The imaging data were analyzed in comparison with the clinical data of the patients.
RESULTSIn 23 patients with lacunar cerebral infarction, intracerebral micro-hemorrhage displayed point-like, round and oval low signal on SWAN. A total of 123 lesions were identified, distributing from the cortical, subcortical, basal ganglia, thalamus, brain stem to the cerebellum. The conventional sequences were more sensitive in detecting the majority of lacunar cerebral infarction than SWAN, while the latter showed better performance in displaying cerebral micro-hemorrhage, tiny blood vessels and small vascular malformations as well as other small vascular diseases. SWAN was superior to other sequences in showing lacunar cerebral infarction complicated by cerebral micro-hemorrhages.
CONCLUSIONMRI SWAN can better display lacunar cerebral infarction associated with cerebral micro-hemorrhages and small veins in the infract region. Identification of the micro-hemorrhages in lacunar cerebral infarction can be critical in determining the proper treatments. Patients with lacunar cerebral infarction are likely to have cerebral micro-hemorrhages in close relation to the number of lacunar infarction sites. The cerebral micro-hemorrhages and lacunar cerebral infraction are both signs of micro-vessel damage of the brain.
Aged ; Cerebral Infarction ; classification ; complications ; diagnosis ; Diabetes Mellitus, Type 2 ; complications ; Female ; Humans ; Hypertension ; complications ; Magnetic Resonance Imaging ; methods ; Male ; Sensitivity and Specificity