2.Intraoperative vessel thrombosis and its management in free flap transfers in head and neck region.
Chi MAO ; Guang-Yan YU ; Xin PENG
Chinese Journal of Stomatology 2009;44(5):304-305
Adolescent
;
Adult
;
Aged
;
Aged, 80 and over
;
Blood Vessels
;
injuries
;
Child
;
Child, Preschool
;
Female
;
Free Tissue Flaps
;
blood supply
;
Head
;
surgery
;
Humans
;
Male
;
Middle Aged
;
Neck
;
surgery
;
Tissue Transplantation
;
adverse effects
;
methods
;
Young Adult
5.Construction and expression of a novel bisbicistronic expression vector: pCMV-Myc-IRES-EGFP.
Fei YAN ; Xin-Yu ZHAO ; Hong-Xin DENG ; Yu-Quan WEI
Chinese Journal of Biotechnology 2007;23(3):423-428
It is often necessary to construct more than one recombinant plasmids when investigating the characteristics, physchemical features and functional mechanisms of genes or proteins. Repeated sub-cloning procedures including design of primers, enzyme digestion, ligation and verification of recombinant plasmids, have to be involved with. For this reason, it has become a tendency to developing new genetic vectors which can be used in multitude of experiments. Therefore, by using pIRES vector as a backbone, here we reported the construction of a mammalian expression vector: pCMV-Myc-IRES-EGFP which contains the N-terminal c-Myc epitope tag and the enhanced green fluorescent protein (EGFP) translated in an IRES-dependent manner. This novel vector can be used to testify the efficiency of cell transfection, to collect successfully transfected cell population via cytometry, to conduct transcription and translation in vitro, to purify target proteins or to trap their interactional proteins. The availability of this vector can facilitate function study of genes.
Apoptosis Regulatory Proteins
;
genetics
;
metabolism
;
Base Sequence
;
Blotting, Western
;
Cell Line
;
Cloning, Molecular
;
Gene Expression
;
Genes, myc
;
genetics
;
Genetic Vectors
;
genetics
;
Green Fluorescent Proteins
;
genetics
;
metabolism
;
Humans
;
Microscopy, Fluorescence
;
Molecular Sequence Data
;
Recombinant Fusion Proteins
;
genetics
;
metabolism
;
Transfection
6.Study on material base of Carthamus tinctorius with antioxidant effect based on selective knock-out.
Lin-Yan WANG ; Yu-Ping TANG ; Xin LIU ; Ya-Hui GE ; Shu-Jiao LI ; Er-Xin SHANG ; Jin-Ao DUAN
China Journal of Chinese Materia Medica 2014;39(7):1285-1289
OBJECTIVETo establish a method for studying efficacious materials of traditional Chinese medicines from an overall perspective.
METHODCarthamus tinctorius was taken the example. Its major components were depleted by preparing liquid chromatography. Afterwards, the samples with major components depleted were evaluated for their antioxidant effect, so as to compare and analyze the major efficacious materials of C. tinctorius with antioxidant activity and the contributions.
RESULTSeven major components were depleted from C. tinctorius samples, and six of them were identified with MS data and control comparison. After all of the samples including depleted materials are compared and evaluated for their antioxidant effect, the findings showed that hydroxysafflor yellow A, anhydrosafflor yellow B and 6-hydroxykaempferol-3, 6-di-O-glucoside-7-O-glucuronide were the major efficacious materials.
CONCLUSIONThis study explored a novel and effective method for studying efficacious materials of traditional Chinese medicines. Through this method, we could explain the direct and indirect contributions of different components to the efficacy of traditional Chinese medicines, and make the efficacious material expression of traditional Chinese medicines clearer.
Alkalies ; chemistry ; Antioxidants ; chemistry ; Carthamus tinctorius ; chemistry ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Glucosides ; chemistry ; Mass Spectrometry
7.Recent progress in the study of HIV-1 transcription factor NF-kappaB and its inhibitors.
Acta Pharmaceutica Sinica 2007;42(10):1007-1012
Human immunodeficiency virus type 1 (HIV-1) transcription is a crucial step in the viral replication cycle, which is considered to be a potential target for inhibition of HIV-1 replication. Among the factors involved in this step, the cellular protein nuclear factor NF-kappaB is the most powerful inducer of HIV-1 transcription. HIV-1 transcription is initiated by the binding of NF-kappaB to the enhancer region in the long terminal repeat (LTR) of HIV-1. Several compounds suppress HIV-1 transcription through the inhibition of NF-kappaB activation. The mechanisms of NF-kappaB in the transcription of HIV-1 and progress of the current inhibitors of NF-kappaB are reviewed.
Anti-HIV Agents
;
pharmacology
;
HIV Long Terminal Repeat
;
HIV-1
;
genetics
;
Humans
;
I-kappa B Kinase
;
metabolism
;
I-kappa B Proteins
;
metabolism
;
NF-KappaB Inhibitor alpha
;
NF-kappa B
;
antagonists & inhibitors
;
metabolism
;
Nicotinic Acids
;
pharmacology
;
Nitriles
;
pharmacology
;
Transcription, Genetic
;
drug effects
;
Virus Replication
8.Designation and evaluation of antisense oligodeoxynucleotides targeted to glial glutamate transporter-1a.
Li-zhe LIU ; Min ZHANG ; Yi-xian LIU ; Xin CUI ; Yu-yan HU ; Wen-bin LI
Chinese Journal of Applied Physiology 2015;31(3):238-243
OBJECTIVEThe present study was undertaken to design antisense oligodeoxynucleotides (AS-ODNs) of glial glutamate transporter-la (GLT-1a) and to evaluate the effectiveness of the designed AS-ODNs on the expression of GLT-1a.
METHODSFive sequences of GLT-1a AS-ODNs were designed according to the C terminus specific sequences of GLT-1a mRNA using antisense design software of IDT Com- pany. Western blot analysis was used to evaluate the inhibition effects of the five GLT-1a AS-ODNs on the expression of GLT-la.
RESULTSThe sequence of GLT-1a AS-ODNs with sequence of 5'-GGTTCTTCCTCAACACTGCA-3' could specifically inhibit the expression of GLT-1a in the hippocampal CA1 subfield of rats, while it had no effect on the expression of GLT-1b. This sequence showed similar inhibition on the expression of GLT-la in sham and ceftriaxone (Cef)-treated rats. It could also significantly inhibit the cerebral ischemic preconditioning (CIP)-induced up-regulation in the expression of GLT-1a. The magnitude of the inhibition in sham, Cef- or CIP-treated rats was similar by more than 60%.
CONCLUSIONFrom the designed five sequences of GLT-1a AS-ODNs, we obtained an effective sequence which can specifically inhibit the expression of GLT-1a.
Animals ; CA1 Region, Hippocampal ; metabolism ; Excitatory Amino Acid Transporter 2 ; antagonists & inhibitors ; metabolism ; Ischemic Preconditioning ; Oligonucleotides, Antisense ; genetics ; RNA, Messenger ; Rats ; Up-Regulation
9.Treatment of hypertension by acupuncture method of "activating blood and dispersing wind, harmonizing Gan-Pi": an analysis of its principle of the circular motion of ancient Chinese medicine.
Zhen-Jie LI ; Yan-Jun ZHANG ; Li-Li ZHANG ; Xin DU ; Shu WANG ; Yu-Zheng DU
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(3):359-361
Hypertension is one of main risk factors for the occurrence and death of stroke and coronary heart disease. Its prevalence rate is rising year by year. It severely threatens the health of the human beings. The acupuncture method of "activating blood and dispersing wind, harmonizing Gan-Pi" for treating hypertension launched by Academician SHI Xue-min has aroused great attention due to good cur- ative effect and less adverse reactions. In this paper principles of the circular motion covered by the acupuncture method of "activating blood and dispersing wind, harmonizing Gan-Pi" were clarified.
Acupuncture
;
Acupuncture Therapy
;
methods
;
Coronary Artery Disease
;
Humans
;
Hypertension
;
therapy
;
Medicine, Chinese Traditional
;
Risk Factors
;
Stroke
;
Wind
10.Huikangling Tablet Intervened Peripheral Blood Micrometastasis of Differentiated Thyroid Carcinoma.
Qin-jiang LIU ; Yu-jie WANG ; You-xin TIAN ; Jun WANG ; Feng DONG ; Yan DENG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(11):1302-1306
OBJECTIVETo observe the clinical effect of Huikangling Tablet (HT, extracted from Scabrous Patrinia root) on peripheral blood micrometastasis of differentiated thyroid carcinoma (DTC) patients.
METHODSTotally 87 DTC patients with positive micrometastasis were randomly assigned to the treatment group (45 cases) and the control group (42 cases). DTC endocrine inhibition treatment standards were executed in all patients. They all took levothyroxine sodium (50 microg/tablet, from low dose, 25 microg each time, once per day, 0.5 h before breakfast), and its dosage was gradually added one week later. The dosage was adjusted according to tested results of TSH combined recurrence risk stratification and endocrine suppression induced adverse reactions risk stratification. Patients in the treatment group took HT (0.4 g per tablet, 3 tablets each time, three times per day for a total of 12 weeks) combined TSH suppression therapy, while those in the control group only received TSH suppression therapy. Peripheral micrometastatic cytokeratin 19 (CK19) and polymorphic epithelial mucin1 (MUC1) were detected by FCM at week 4 and 12. Meanwhile, distant metastasis and adverse reactions were observed.
RESULTSAfter 4-week treatment positive micrometastasis was shown in 18 cases (40%) of the treatment group and 29 cases (69%) in the control group with statistical difference (chi2 = 5.68, P < 0.05). After 12-week treatment positive micrometastasis was shown in 7 cases (15.6%) of the treatment group and 17 cases (44.7%) in the control group with statistical difference (chi2 = 8.49, P < 0.01). Pulmonary metastasis occurred in 2 cases and bone metastasis in 1 case of the control group at follow-ups. Cervical lymph node metastasis without accompanied recurrence of thyroid cancer occurred in one case of the treatment group. No obvious liver or renal abnormalities occurred.
CONCLUSIONHT inhibited peripheral blood micrometastasis of DTC patients and its mechanism needed to be further studied.
Antineoplastic Agents ; pharmacology ; therapeutic use ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Neoplasm Micrometastasis ; drug therapy ; Neoplasm Recurrence, Local ; Tablets ; Thyroid Neoplasms ; drug therapy