4.Medulloblastoma with extensive nodularities: report of a case.
Qiu-ping GUI ; Xin SONG ; Huai-yu TONG
Chinese Journal of Pathology 2007;36(9):644-645
Cerebellar Neoplasms
;
diagnosis
;
pathology
;
radiotherapy
;
surgery
;
Follow-Up Studies
;
Humans
;
Infant
;
Magnetic Resonance Imaging
;
Male
;
Medulloblastoma
;
diagnosis
;
pathology
;
radiotherapy
;
surgery
5.Successful treatment of a patient with craniocervical penetrating injury by a steel bar.
De-zhi YU ; Jian-xin QIU ; Hong-wei CHENG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(5):424-424
Adult
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Fluoroscopy
;
Foreign Bodies
;
therapy
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Humans
;
Male
;
Trauma, Nervous System
;
therapy
;
Wounds, Penetrating
;
therapy
9.Induction of silencing effect of Swedish mutant amyloid precursor protein by RNA interference.
Xin, QIU ; Guohua, CHEN ; Ji'an, PAN ; Yu, CHEN ; Suming, ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2006;26(4):399-401
Over-expression of APP and Swedish mutation could cause some familial early onset AD. In this study, a primary screening was conducted of effective small interference RNAs (siRNAs) targeted wild type APP (APPwt) and Swedish mutant APP (APPswe). One siRNA targeting APPwt and the other siRNA targeting APPswe were designed. All these siRNAs were endogenously expressed by siRNAs expressing plasmids. COS-7 cells were transiently co-transfected with APP-GFP recombinant plasmids and siRNA expression vector. The silencing effect of each siRNA was quantitatively assessed by the level of expression of green fluorescent protein (GFP). It was found that the siRNAs silenced APPwt and APPswe to different degrees. siRNA directed against APPswe was more effective in suppressing the expression of fusion gene of APPswe than that of APPwt. The silencing effect of siRNA directed against APPswe indicating allele-specific silencing property of the siRNAs. Therefore, siRNAs directed against APP play an important role both in the therapeutic study of Alzheimer disease and functional exploration ofAPP gene.
10.Development of Multiplex Real-time PCR for Detection of Toxigenic Vibrio cholerae and Virbio parahaemolyticus
Wei ZHANG ; Jin-Cao PAN ; Dong-Mei MENG ; Xin-Fen YU ; Hao-Qiu WANG ; Wei ZHENG ;
Microbiology 1992;0(05):-
A multiplex real-time PCR was developed to detect ctxA of Vibrio cholerae, gyrB and tdh of Vibrio parahaemolyticus simultaneously. The multiplex real-time PCR were evalidated by detection for the three genes in 47 toxigenic V. cholerae O1 and O139 strains (ctxA+; O1=3, O139=44), 25 non-toxigenic V. cholerae strains (ctxA-; O1=12, O139=6, non-O1 and non-O139=7), 116 V. parahaemolyticus strains with or without tdh (73 or 43) and 9 other bacteria strains. The specificity and sensitivity of the multiplex real-time PCR in detection for the ctxA and the tdh genes in the strains tested were both 100.0%, compared to the results by routine PCRs. In the detection for V. parahaemolyticus specific gyrB using the multiplex real-time PCR, all of 116 V. parahaemolyticus strains were positive, and 9 other strains and 72 V. cholerae strains were all negative. The multiplex real-time PCR is a sensitive, specific and quick assay not only for detecting virulence genes of V. cholerae and V. parahaemolyticus but also for identifying V. parahaemolyticus at species level. In addition, two real-time PCRs for detection of V. parahaemolyticus virulence genes trh1 and trh2 were also developed.